##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938541_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1176603 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.03750287905096 31.0 31.0 34.0 28.0 34.0 2 31.19085451932385 31.0 31.0 34.0 28.0 34.0 3 31.23046431124177 31.0 31.0 34.0 27.0 34.0 4 34.890257801484445 37.0 35.0 37.0 32.0 37.0 5 34.91557390215731 37.0 35.0 37.0 32.0 37.0 6 34.93648324881035 37.0 35.0 37.0 32.0 37.0 7 34.86220245911323 37.0 35.0 37.0 32.0 37.0 8 34.820093948426106 37.0 35.0 37.0 32.0 37.0 9 36.3012723917923 39.0 35.0 39.0 32.0 39.0 10-11 36.120155651481426 39.0 35.0 39.0 30.5 39.0 12-13 35.96667100117882 38.0 35.0 39.0 30.0 39.0 14-15 36.9185791639151 39.0 36.0 41.0 31.0 41.0 16-17 36.76268758451236 39.0 36.0 41.0 30.0 41.0 18-19 36.675427480637055 39.0 36.0 40.0 30.0 41.0 20-21 36.70059909757157 39.0 36.0 40.5 30.0 41.0 22-23 36.76652830223958 39.0 36.0 41.0 30.0 41.0 24-25 36.70083409612248 39.0 36.0 41.0 30.0 41.0 26-27 36.52586938840034 39.0 36.0 41.0 30.0 41.0 28-29 36.31866993369896 39.0 35.0 40.5 29.5 41.0 30-31 36.02138146851571 38.5 35.0 40.0 28.5 41.0 32-33 35.71595686905439 38.0 35.0 40.0 27.0 41.0 34-35 35.47065110321833 38.0 34.5 40.0 27.0 41.0 36-37 35.14193827484716 38.0 34.0 40.0 25.5 41.0 38-39 34.7958389533258 38.0 34.0 40.0 25.0 41.0 40-41 34.614984408504824 38.0 33.0 40.0 24.0 41.0 42-43 34.434514445399174 38.0 33.0 40.0 24.0 41.0 44-45 34.12238452562164 38.0 33.0 40.0 23.5 41.0 46-47 33.63800874211607 38.0 33.0 40.0 20.0 41.0 48-49 33.24792304626115 38.0 32.0 40.0 17.0 41.0 50-51 32.52160711811885 37.0 31.0 39.5 15.0 40.5 52-53 32.55847851824277 37.0 31.0 39.5 14.5 40.0 54-55 32.64999791773436 37.0 31.0 40.0 11.0 41.0 56-57 32.30827390377213 37.0 31.0 40.0 8.5 41.0 58-59 31.8725020248971 36.5 30.5 40.0 7.5 41.0 60-61 31.445372398336566 36.0 29.5 39.0 2.0 41.0 62-63 30.88216713708872 36.0 28.5 39.0 2.0 40.0 64-65 30.382160337853975 35.0 28.0 39.0 2.0 40.0 66-67 29.791440698349398 35.0 28.0 38.0 2.0 40.0 68-69 29.152233591109322 34.0 26.5 38.0 2.0 40.0 70-71 28.5362739173706 34.0 26.0 37.5 2.0 40.0 72-73 27.90474569587193 34.0 25.5 37.0 2.0 39.0 74-75 27.211004051494005 33.0 23.0 36.0 2.0 39.0 76-77 26.454632956060795 32.5 21.0 36.0 2.0 38.5 78-79 25.642766506629677 32.0 19.0 35.0 2.0 38.0 80-81 24.858919278635188 31.0 12.5 35.0 2.0 37.0 82-83 24.145544418975646 31.0 7.5 35.0 2.0 36.5 84-85 23.378899679841034 30.0 2.0 34.0 2.0 36.0 86-87 22.594946638755808 29.5 2.0 34.0 2.0 35.5 88-89 21.74224271058292 29.0 2.0 34.0 2.0 35.0 90-91 20.905269661899553 29.0 2.0 34.0 2.0 35.0 92-93 20.09801436848283 27.0 2.0 34.0 2.0 35.0 94-95 19.156725335563483 26.0 2.0 34.0 2.0 35.0 96-97 17.92219125737398 24.0 2.0 33.0 2.0 35.0 98-99 16.655612810778145 10.5 2.0 32.0 2.0 34.5 100-101 14.010859227793912 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.8280189041436863 1101 2 0.8226061227391419 1101 3 0.8532549458883061 1101 4 0.6896833137748857 1101 5 0.8634518441028973 1101 6 0.8080462707116709 1101 7 0.7702773641665175 1101 8 0.5245542439413597 1101 9 0.8226816117932501 1101 10-11 1.0458182460190812 1101 12-13 1.0048644827421 1101 14-15 1.3262038529184679 1101 16-17 1.3451151946445634 1101 18-19 1.4910478202602135 1101 20-21 1.4375386571792745 1101 22-23 1.3226380643678297 1101 24-25 1.3951444642324589 1101 26-27 1.3943465227033585 1101 28-29 1.4594248624108346 1101 30-31 1.4150768816427615 1101 32-33 1.6117491706030975 1101 34-35 1.6105217789990576 1101 36-37 1.5569950087567719 1101 38-39 1.5549522085445346 1101 40-41 1.7900311520200916 1101 42-43 1.703579897064074 1101 44-45 1.9837788015845206 1101 46-47 2.223636612110276 1101 48-49 2.230848307767509 1101 50-51 2.244077786283654 1101 52-53 2.5510249110984446 1101 54-55 2.762970779679698 1101 56-57 2.8512631345590904 1101 58-59 3.0019847050943724 1101 60-61 2.8227029083553994 1101 62-63 2.8048820803606915 1101 64-65 3.0767068908080404 1101 66-67 3.210058198324244 1101 68-69 3.4374942923752627 1101 70-71 3.730686575850715 1101 72-73 3.7101346984426726 1101 74-75 3.955896935960002 1101 76-77 4.245749399820916 1101 78-79 4.463543637247039 1101 80-81 4.933914111403865 1101 82-83 5.217053784612617 1101 84-85 4.980415452383927 1101 86-87 5.256994225106901 1101 88-89 5.752267959450581 1101 90-91 5.850193111052558 1101 92-93 5.715815251485292 1101 94-95 6.18129571338546 1101 96-97 6.595179272193917 1101 98-99 6.74605505843412 1101 100-101 6.2735337472058585 1102 1 0.5331407750264248 1102 2 0.5263017749130547 1102 3 0.5403596889713107 1102 4 0.6096009685838482 1102 5 0.5027008559606045 1102 6 0.6032603682083746 1102 7 0.5061633984221174 1102 8 0.6002162992773279 1102 9 0.5884095432820544 1102 10-11 0.6089852420665167 1102 12-13 0.7726246935457866 1102 14-15 1.0191221664889483 1102 16-17 1.139963679493043 1102 18-19 1.0492082286923576 1102 20-21 0.7434922144915319 1102 22-23 0.647353150006829 1102 24-25 0.6953914998055879 1102 26-27 0.858644941675692 1102 28-29 0.8843754552962011 1102 30-31 1.0392023757139057 1102 32-33 0.9599787489957166 1102 34-35 1.071052082029368 1102 36-37 1.292869514685627 1102 38-39 1.2278211150003955 1102 40-41 1.4545041427974326 1102 42-43 1.7041266691325845 1102 44-45 1.6498031757610647 1102 46-47 1.9810114474198954 1102 48-49 1.745507398676601 1102 50-51 2.023050118299466 1102 52-53 1.914789733232837 1102 54-55 1.8444645214451754 1102 56-57 2.105316164862117 1102 58-59 2.287419487703069 1102 60-61 2.171029654073454 1102 62-63 2.468238470347515 1102 64-65 2.282121910570879 1102 66-67 2.5688477240159493 1102 68-69 2.7484066770919924 1102 70-71 2.887009039618828 1102 72-73 3.0680513651093335 1102 74-75 3.189976316724163 1102 76-77 3.37911237742302 1102 78-79 4.017611489684455 1102 80-81 4.369475705606764 1102 82-83 4.48437921280761 1102 84-85 4.073740551198156 1102 86-87 4.343183289665532 1102 88-89 4.8347761939696845 1102 90-91 5.066690146625682 1102 92-93 4.928020455701368 1102 94-95 5.231216662002062 1102 96-97 5.345589351245298 1102 98-99 5.795052094007243 1102 100-101 5.353234010710471 1103 1 0.7684950946198761 1103 2 0.6999654300984481 1103 3 0.5839908766242381 1103 4 0.6098997639913364 1103 5 0.4591661298171772 1103 6 0.5574835001488978 1103 7 0.746467840356992 1103 8 0.6620434214305391 1103 9 0.8228980620097062 1103 10-11 0.7904069906078348 1103 12-13 0.9100376429152632 1103 14-15 1.0032601299747412 1103 16-17 1.1670199565493249 1103 18-19 1.2885586427710294 1103 20-21 1.1691403887810097 1103 22-23 0.9442181509479184 1103 24-25 1.088434507522507 1103 26-27 1.1525066958635293 1103 28-29 1.5355071134930753 1103 30-31 1.515921037486919 1103 32-33 1.491511075365004 1103 34-35 1.6487035971808837 1103 36-37 1.462146523908288 1103 38-39 1.6703418189341406 1103 40-41 1.7147714117603527 1103 42-43 1.732302840787014 1103 44-45 1.7714411392468534 1103 46-47 2.1595240579977215 1103 48-49 1.9526231995424013 1103 50-51 2.127779084984958 1103 52-53 2.074466469539999 1103 54-55 2.0981006498095667 1103 56-57 2.1386224419183932 1103 58-59 2.6185647916744585 1103 60-61 2.576902042554533 1103 62-63 2.8136483141269224 1103 64-65 2.940299964401106 1103 66-67 2.984560362826411 1103 68-69 3.3376241625051364 1103 70-71 3.3800155801797125 1103 72-73 3.4185113218192917 1103 74-75 3.656935896998963 1103 76-77 3.9622645513360624 1103 78-79 4.231400780104181 1103 80-81 4.416121903611657 1103 82-83 4.724196641755473 1103 84-85 4.875155712124187 1103 86-87 4.924180372293048 1103 88-89 5.310146747329373 1103 90-91 5.4926606435200895 1103 92-93 5.604365035035077 1103 94-95 5.691295713385465 1103 96-97 6.083036415051058 1103 98-99 6.580925188304249 1103 100-101 6.451650630322744 1104 1 0.6264378764757019 1104 2 0.49494725986288657 1104 3 0.5786121973325145 1104 4 0.5510028637900888 1104 5 0.4374834646562533 1104 6 0.4794448721102711 1104 7 0.7176321832604629 1104 8 0.5536025869027483 1104 9 0.7212830103946501 1104 10-11 0.7386884193240348 1104 12-13 0.7302612598780129 1104 14-15 0.8835313080720013 1104 16-17 1.0238822971073205 1104 18-19 1.1361299120814365 1104 20-21 1.202626829876145 1104 22-23 1.0911588913669235 1104 24-25 1.125784476394216 1104 26-27 1.0582686874950937 1104 28-29 1.0877617429216215 1104 30-31 1.0717065563159096 1104 32-33 1.075955616331278 1104 34-35 1.3515007999780835 1104 36-37 1.6077733608394738 1104 38-39 1.5640111930383043 1104 40-41 1.6574026935220658 1104 42-43 1.6621812956654693 1104 44-45 1.7262385157833648 1104 46-47 1.8916162411768553 1104 48-49 2.0936470307836217 1104 50-51 2.004237409269365 1104 52-53 2.116085719854908 1104 54-55 2.4659458480895466 1104 56-57 2.6241637122422716 1104 58-59 2.8384228321846763 1104 60-61 2.7815369004502664 1104 62-63 2.8247741448179724 1104 64-65 2.8602991680959633 1104 66-67 3.1000977240159493 1104 68-69 3.3985599658322414 1104 70-71 3.4898170106333204 1104 72-73 3.5461380429242766 1104 74-75 3.6771906422537057 1104 76-77 3.852844261480989 1104 78-79 4.155083619004412 1104 80-81 4.606690031136306 1104 82-83 4.768102735661564 1104 84-85 4.993995567920564 1104 86-87 4.736647615195075 1104 88-89 4.999457074683175 1104 90-91 5.500950180070497 1104 92-93 5.595070344218648 1104 94-95 5.7491932506308245 1104 96-97 5.9984948696399485 1104 98-99 6.051009451866772 1104 100-101 5.376157645047149 1105 1 0.601970097937464 1105 2 0.5655970092345832 1105 3 0.6555144924726228 1105 4 0.619681807410494 1105 5 0.3900067887440031 1105 6 0.44144538195667593 1105 7 0.5589570357790805 1105 8 0.6204132482344988 1105 9 0.7000447209293554 1105 10-11 0.6254145403626836 1105 12-13 0.8630828302603746 1105 14-15 1.040175382631496 1105 16-17 1.0480691752124613 1105 18-19 1.2726080371766813 1105 20-21 1.185712190356952 1105 22-23 1.0270588672600525 1105 24-25 0.8909153462088142 1105 26-27 1.1887010455624392 1105 28-29 1.1912110339857307 1105 30-31 1.1759916214183121 1105 32-33 1.4053828981017773 1105 34-35 1.6640685139542484 1105 36-37 1.7767306418064024 1105 38-39 1.6786386587968494 1105 40-41 1.9516644276094581 1105 42-43 1.8941534453053919 1105 44-45 1.633763737940619 1105 46-47 2.006398531218501 1105 48-49 2.0258253357105716 1105 50-51 1.9027239412885564 1105 52-53 2.123375969319426 1105 54-55 2.35922030894082 1105 56-57 2.688105794798595 1105 58-59 2.500501689352866 1105 60-61 2.914074453132084 1105 62-63 3.0125174648601956 1105 64-65 3.3410535052540666 1105 66-67 3.497048482273936 1105 68-69 3.6498924244834257 1105 70-71 3.8947420853784642 1105 72-73 3.796708849229745 1105 74-75 4.0169634419478015 1105 76-77 4.245004126039184 1105 78-79 4.3959022272899695 1105 80-81 4.584808891851257 1105 82-83 4.915400549695089 1105 84-85 5.084663023371348 1105 86-87 5.53709816424978 1105 88-89 5.95484308937451 1105 90-91 5.856585368339594 1105 92-93 6.236398591095146 1105 94-95 6.2881850709210525 1105 96-97 6.381310551850465 1105 98-99 6.573296528645766 1105 100-101 6.327869440510069 1106 1 0.11523697819495382 1106 2 0.12791518285768433 1106 3 -0.07294011222885288 1106 4 0.06240057957428746 1106 5 0.15456184333313416 1106 6 0.015121938481662767 1106 7 0.27723602785675894 1106 8 0.16151521692265192 1106 9 0.19195649440428753 1106 10-11 -0.04432877884428166 1106 12-13 0.042266915195341426 1106 14-15 0.5272045457057786 1106 16-17 0.28807262671291767 1106 18-19 0.4673256279732456 1106 20-21 0.40432335427726684 1106 22-23 0.07376461351724828 1106 24-25 0.3225980859705899 1106 26-27 0.39919872807630696 1106 28-29 0.4082567460225661 1106 30-31 0.5215117211470286 1106 32-33 0.7916987510818885 1106 34-35 0.46984900554051023 1106 36-37 0.51494204901541 1106 38-39 0.44490332855069425 1106 40-41 0.49628278776791035 1106 42-43 -0.10730186723429114 1106 44-45 0.3958371496716566 1106 46-47 1.0668811660322177 1106 48-49 0.9112832242481517 1106 50-51 0.17111458098768395 1106 52-53 0.6955645924162752 1106 54-55 0.6405102300684291 1106 56-57 0.7701746887119612 1106 58-59 -0.11661817382179862 1106 60-61 1.351231889958047 1106 62-63 1.7156529986887676 1106 64-65 1.7782379507726205 1106 66-67 1.825192522086855 1106 68-69 1.5342405012891263 1106 70-71 2.076787337916759 1106 72-73 2.3808247915840752 1106 74-75 2.446755222466045 1106 76-77 2.7854087793913855 1106 78-79 3.0377315365619495 1106 80-81 3.396479733757804 1106 82-83 3.6375256497500175 1106 84-85 3.6011825218935876 1106 86-87 3.4907729950245354 1106 88-89 3.536077519915519 1106 90-91 4.066012524492635 1106 92-93 4.411462622742317 1106 94-95 4.42031580421758 1106 96-97 4.781815789783014 1106 98-99 4.829355420223049 1106 100-101 4.433909776764784 1107 1 0.4087605309379434 1107 2 0.14622454379177086 1107 3 0.2984403525868693 1107 4 0.21689025157392905 1107 5 0.3200278010406947 1107 6 0.24190691664468034 1107 7 0.36166492397512684 1107 8 0.3431367798265299 1107 9 0.28859070270234355 1107 10-11 0.23319862879419873 1107 12-13 0.27016950666554607 1107 14-15 0.6193557667940652 1107 16-17 0.6373595128263787 1107 18-19 0.5161823896381961 1107 20-21 0.7203586332558345 1107 22-23 0.5406299902051543 1107 24-25 0.7061716771511257 1107 26-27 0.745068711698579 1107 28-29 0.6569727093007813 1107 30-31 0.5400783768580695 1107 32-33 0.6861062280193622 1107 34-35 0.7880202837837516 1107 36-37 0.8569606188046208 1107 38-39 0.8806684166785104 1107 40-41 1.1064516065655425 1107 42-43 1.1513639879731628 1107 44-45 1.2074106795749486 1107 46-47 1.1447819470385028 1107 48-49 1.3359679249923886 1107 50-51 1.2223493173841362 1107 52-53 1.260559899136723 1107 54-55 1.4151941648471649 1107 56-57 1.643008050827028 1107 58-59 1.7346985208838461 1107 60-61 1.80874656864248 1107 62-63 2.03721611146117 1107 64-65 1.9990483979850637 1107 66-67 2.074783078707025 1107 68-69 2.2032598426145 1107 70-71 2.338679996903341 1107 72-73 2.6941478563374055 1107 74-75 2.807283000553305 1107 76-77 2.9967766127395734 1107 78-79 2.818425515237468 1107 80-81 3.3269568745617626 1107 82-83 3.493115387483428 1107 84-85 3.559688777742778 1107 86-87 3.8700115696045074 1107 88-89 4.111711739354888 1107 90-91 4.648194008181747 1107 92-93 4.60024108880587 1107 94-95 4.559395294725178 1107 96-97 4.534018985112578 1107 98-99 5.136424376615938 1107 100-101 4.882648579741746 1108 1 0.30859075165101757 1108 2 0.35954160661010803 1108 3 0.34980919808771915 1108 4 0.4627624926605165 1108 5 0.34779202005597654 1108 6 0.2984275023832197 1108 7 0.20950023806680917 1108 8 0.4451847424750852 1108 9 0.35471973496040476 1108 10-11 0.3613607680132205 1108 12-13 0.4381342774635115 1108 14-15 0.28859388224397975 1108 16-17 0.45353894831025343 1108 18-19 0.6529979668877743 1108 20-21 0.5795107979417438 1108 22-23 0.728679120086305 1108 24-25 0.5278424114465423 1108 26-27 0.685468223583122 1108 28-29 0.8215949503873716 1108 30-31 0.8652455033436368 1108 32-33 0.9714999037409271 1108 34-35 1.0338208992336604 1108 36-37 1.1136005806042775 1108 38-39 1.1147542613304537 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0.6564218148041618 2311 52-53 0.8274687613658216 2311 54-55 0.7474003696975231 2311 56-57 1.0623504785876108 2311 58-59 1.0115925482316328 2311 60-61 1.0651984520274134 2311 62-63 1.1785986579007997 2311 64-65 1.2196837179025053 2311 66-67 1.2741045441353016 2311 68-69 1.292057572232661 2311 70-71 1.3043140268311042 2311 72-73 1.340624894521099 2311 74-75 1.692296222947526 2311 76-77 1.7497155317282207 2311 78-79 1.9173493413468599 2311 80-81 2.0419533270901375 2311 82-83 2.2492641232935426 2311 84-85 2.3963780192288446 2311 86-87 2.824195651131859 2311 88-89 2.8958151965272307 2311 90-91 3.0779827723716338 2311 92-93 3.1083731837491086 2311 94-95 3.3337912570574773 2311 96-97 3.160321874689462 2311 98-99 3.2057074648512334 2311 100-101 2.7922770627138807 2312 1 -0.22462289849811512 2312 2 -0.280431950298933 2312 3 -0.27992298728962695 2312 4 -0.2735723194807491 2312 5 -0.15584108519003337 2312 6 -0.007928865263465923 2312 7 -0.0484483545592056 2312 8 -0.13331677392965702 2312 9 -0.014730975619336562 2312 10-11 -0.23444593424509463 2312 12-13 -0.1489700160923988 2312 14-15 -0.07830275158814004 2312 16-17 -0.047099257569890085 2312 18-19 0.014377268589655046 2312 20-21 -0.18173096209034156 2312 22-23 -0.28968128295151274 2312 24-25 -0.30427278518478573 2312 26-27 -0.2012284578716148 2312 28-29 -0.2366318586458931 2312 30-31 -0.1462168246509421 2312 32-33 0.1978602817142061 2312 34-35 0.20503615351343285 2312 36-37 0.1831255448873108 2312 38-39 0.37370512229744435 2312 40-41 0.2551865521754877 2312 42-43 0.4612294697136434 2312 44-45 0.2089924767981941 2312 46-47 0.14292954140319836 2312 48-49 0.8553316022508071 2312 50-51 0.6696372268430935 2312 52-53 0.7427537378272717 2312 54-55 0.7276599793688945 2312 56-57 0.6861970894930423 2312 58-59 1.0067321798418476 2312 60-61 1.038196303848796 2312 62-63 1.1149248154034268 2312 64-65 1.2551295792307222 2312 66-67 1.288900467166517 2312 68-69 1.5006722255531955 2312 70-71 1.8686935688577044 2312 72-73 1.8335185368265066 2312 74-75 1.8507531908162562 2312 76-77 1.7269110159825303 2312 78-79 1.8708280195314195 2312 80-81 2.017309604799358 2312 82-83 2.124551842110673 2312 84-85 2.2123074492759223 2312 86-87 2.5096282577409355 2312 88-89 2.7015997387823596 2312 90-91 3.0727805236399703 2312 92-93 3.169066999737044 2312 94-95 3.2996096216993713 2312 96-97 3.4513680833827287 2312 98-99 3.2917698991489637 2312 100-101 3.3169408956130066 2313 1 -0.43373414031963975 2313 2 -0.5243720704072778 2313 3 -0.5042783308038494 2313 4 -0.5641343016315119 2313 5 -0.35257477725082964 2313 6 -0.5641089261164254 2313 7 -0.5613142529391197 2313 8 -0.43773405965535517 2313 9 -0.37768655586463495 2313 10-11 -0.41066716887755206 2313 12-13 -0.5645011360627663 2313 14-15 -0.40030421844941344 2313 16-17 -0.5045067254913818 2313 18-19 -0.3733461191337355 2313 20-21 -0.46351628476351436 2313 22-23 -0.5762475204523341 2313 24-25 -0.356222004190073 2313 26-27 -0.25562940479593266 2313 28-29 -0.22422707754952143 2313 30-31 -0.34225248680809983 2313 32-33 -0.35360058867189537 2313 34-35 -0.1452641173475797 2313 36-37 0.0781405765845804 2313 38-39 0.09698845889854368 2313 40-41 0.05217785267146269 2313 42-43 0.25947341164917503 2313 44-45 0.25860887805010435 2313 46-47 0.34008804230001743 2313 48-49 0.4944365264024597 2313 50-51 0.48732615784978606 2313 52-53 0.6825924557033716 2313 54-55 0.6968322919991508 2313 56-57 0.6899150745194405 2313 58-59 0.868043895125524 2313 60-61 1.1045763153222659 2313 62-63 1.5637535048863214 2313 64-65 1.4908785135777904 2313 66-67 1.4147056069903492 2313 68-69 1.464265745221482 2313 70-71 1.6116678842619265 2313 72-73 1.8008200566981216 2313 74-75 1.7031611670560096 2313 76-77 1.8658471058531987 2313 78-79 1.813451595332566 2313 80-81 2.141125949410096 2313 82-83 1.9778439290476229 2313 84-85 2.233469827914938 2313 86-87 2.521032741108037 2313 88-89 2.597360001365054 2313 90-91 2.6263500071159953 2313 92-93 2.573392425090507 2313 94-95 2.8963934194177483 2313 96-97 3.3119436448928674 2313 98-99 3.6831776865847843 2313 100-101 3.5278158202729824 2314 1 -0.6698891939984222 2314 2 -0.595069384281139 2314 3 -0.5311869772431663 2314 4 -0.4138239901536309 2314 5 -0.42183960391866293 2314 6 -0.5798675003846299 2314 7 -0.3821974620992634 2314 8 -0.2881162992440025 2314 9 -0.6585621834131032 2314 10-11 -0.7152936530023339 2314 12-13 -0.6430443245192734 2314 14-15 -0.652990587586423 2314 16-17 -0.6725688218070047 2314 18-19 -0.5927948970848504 2314 20-21 -0.6128442672286027 2314 22-23 -0.7322853507505869 2314 24-25 -0.5619467285061575 2314 26-27 -0.4485644119158465 2314 28-29 -0.5640788959213197 2314 30-31 -0.5591260682934163 2314 32-33 -0.5424878170582303 2314 34-35 -0.5405248810491656 2314 36-37 -0.43192996642405745 2314 38-39 -0.5345166611193477 2314 40-41 -0.37178064772462704 2314 42-43 -0.3535843349597556 2314 44-45 -0.2843675606336049 2314 46-47 -0.1886467519515591 2314 48-49 -0.007368966378002995 2314 50-51 -0.13806871775832263 2314 52-53 0.11411738025175566 2314 54-55 0.02084413097738036 2314 56-57 -0.008844651570534268 2314 58-59 0.4925931294313415 2314 60-61 0.6379518092205281 2314 62-63 0.7795062471455445 2314 64-65 0.8738264483176081 2314 66-67 1.1265679132583024 2314 68-69 1.3436962495444575 2314 70-71 1.3854369658663117 2314 72-73 1.435852327147817 2314 74-75 1.478537696135863 2314 76-77 1.6184787999060077 2314 78-79 2.120475278149037 2314 80-81 2.08358649829934 2314 82-83 2.2586223564463985 2314 84-85 2.4928229123186902 2314 86-87 2.6695910198877826 2314 88-89 2.8554689238527935 2314 90-91 3.0423374877367166 2314 92-93 2.7552004450748555 2314 94-95 3.0858425861694627 2314 96-97 3.0500147565223834 2314 98-99 2.848020027941992 2314 100-101 2.574823635165016 2315 1 -0.6720347400877706 2315 2 -0.4929957655378061 2315 3 -0.5296361205590152 2315 4 -0.4362540297627717 2315 5 -0.23942277144678314 2315 6 -0.19102961799443108 2315 7 -0.28666419938402043 2315 8 -0.37773028506228457 2315 9 -0.4158742854959243 2315 10-11 -0.5518571706177795 2315 12-13 -0.5316532913799179 2315 14-15 -0.6423463223193551 2315 16-17 -0.5465692342231847 2315 18-19 -0.41557008976052856 2315 20-21 -0.41304427680255884 2315 22-23 -0.6171706045399716 2315 24-25 -0.3959970850129366 2315 26-27 -0.43519464030357824 2315 28-29 -0.3497071739241946 2315 30-31 -0.4547289262393619 2315 32-33 -0.5927801906729186 2315 34-35 -0.8399595885712117 2315 36-37 -0.6017711739197225 2315 38-39 -0.30040112812665853 2315 40-41 -0.5413281928050324 2315 42-43 -0.40682100415902056 2315 44-45 -0.45077165975587263 2315 46-47 -0.2731669069513103 2315 48-49 -0.4469837500252112 2315 50-51 -0.18766636649510815 2315 52-53 -0.045851921746134394 2315 54-55 0.11126238972190095 2315 56-57 0.2514691284078836 2315 58-59 0.4698289222462222 2315 60-61 0.6170609657189665 2315 62-63 0.7798177072829446 2315 64-65 0.6963113540080919 2315 66-67 0.9555778321129615 2315 68-69 1.0994304915026518 2315 70-71 1.24272040747935 2315 72-73 1.566338474996563 2315 74-75 1.4409366326912796 2315 76-77 1.4285378269720468 2315 78-79 1.8690600523818652 2315 80-81 1.9826080531819947 2315 82-83 2.086401470758098 2315 84-85 2.3307863128374002 2315 86-87 2.8175993320377373 2315 88-89 2.966220466424332 2315 90-91 3.110947706575054 2315 92-93 3.029534227238102 2315 94-95 3.335756052417004 2315 96-97 3.6149307205881662 2315 98-99 3.566247104782736 2315 100-101 3.3116207939121036 2316 1 -0.9074475054662798 2316 2 -0.7049468952053957 2316 3 -0.6677446092058652 2316 4 -0.5419183472068738 2316 5 -0.6683396433655915 2316 6 -0.5158807647324934 2316 7 -0.5905116021689452 2316 8 -0.5237951554357707 2316 9 -0.6055773233987978 2316 10-11 -0.4913388057382946 2316 12-13 -0.5963760630090462 2316 14-15 -1.2709190893920734 2316 16-17 -0.9165887946777005 2316 18-19 -0.6271525356644574 2316 20-21 -0.8919141086519531 2316 22-23 -1.0315284787138808 2316 24-25 -1.012774859510671 2316 26-27 -0.6430174102641573 2316 28-29 -0.7858183527752871 2316 30-31 -0.9572002946882492 2316 32-33 -0.8127762631800906 2316 34-35 -0.8752842420598128 2316 36-37 -0.6603607676038976 2316 38-39 -0.745260604743315 2316 40-41 -0.838873638132668 2316 42-43 -0.8133331979974727 2316 44-45 -0.4447174167159389 2316 46-47 -0.3412351067092416 2316 48-49 -0.12026543982258175 2316 50-51 -0.0468750536985425 2316 52-53 0.029846652163250553 2316 54-55 0.00932181037819646 2316 56-57 0.24375757323623048 2316 58-59 0.35702548812862744 2316 60-61 0.43723056666772564 2316 62-63 0.4354953088939837 2316 64-65 0.4694578918461474 2316 66-67 0.6766279743883139 2316 68-69 0.9577137792505397 2316 70-71 0.9580683050513628 2316 72-73 1.0412195271539275 2316 74-75 1.402140447750007 2316 76-77 1.6283602555231198 2316 78-79 1.8130653634816518 2316 80-81 2.0634736546338814 2316 82-83 2.1852504329887097 2316 84-85 2.189610914344481 2316 86-87 2.54517604328872 2316 88-89 2.510470539904386 2316 90-91 2.7092069031332358 2316 92-93 3.0556587929356844 2316 94-95 3.1719571400501536 2316 96-97 2.988540535726468 2316 98-99 3.088160204511535 2316 100-101 3.3464590030267143 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3883.0 3 1346.0 4 1342.0 5 1642.0 6 1846.0 7 2398.0 8 3143.0 9 4002.0 10 5236.0 11 7136.0 12 9041.0 13 11048.0 14 11680.0 15 13553.0 16 15636.0 17 18323.0 18 17062.0 19 15636.0 20 16652.0 21 18109.0 22 20005.0 23 22287.0 24 24479.0 25 27638.0 26 30776.0 27 34022.0 28 38423.0 29 43001.0 30 48850.0 31 54873.0 32 62429.0 33 72346.0 34 84795.0 35 100685.0 36 114922.0 37 127980.0 38 83087.0 39 7289.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.433565288998345 19.989702225529786 14.787791681478243 22.788940803993626 2 14.192229212718926 20.644533242645807 43.96930794082639 21.193929603808876 3 18.684497380320348 26.842065882264915 30.366772156742037 24.1066645806727 4 11.837515921725482 17.713121542498463 38.55472396957313 31.894638566202914 5 11.63300536596566 38.02037536247906 34.64398391060692 15.702635360948355 6 28.336987191370888 36.65596304529367 20.450776664518006 14.556273098817444 7 26.732458620018313 32.96404708930968 22.871743948690437 17.431750341981562 8 24.6334141308682 37.66641642386313 21.057464306392415 16.642705138876256 9 26.04983071771946 18.006924333966477 22.647520452462373 33.295724495851694 10-11 23.561145956653608 26.639305063455254 30.339752895391815 19.45979608449932 12-13 25.96750538813717 24.822584690463742 29.580681774705724 19.62922814669336 14-15 20.966341939910105 29.085729393991688 27.063873886204792 22.884054779893408 16-17 19.600196539644138 30.342252708158178 30.37625610679787 19.68129464539981 18-19 20.415531575477523 27.533355634890917 32.94056238180635 19.11055040782521 20-21 20.863505391647983 27.813175125045593 31.337237527536388 19.98608195577004 22-23 20.01977480022223 29.351805683257183 31.53319739220885 19.09522212431174 24-25 20.54711176416567 27.947333870742813 32.671817974646515 18.833736390444997 26-27 19.0575813397058 29.39117074504199 32.109175951225296 19.442071964026912 28-29 19.106088682062982 29.66789818486036 30.720661321444105 20.505351811632554 30-31 19.768479904912756 28.040662039835638 32.05789405220106 20.132964003050546 32-33 19.06536947083881 28.89329845918675 32.77073054195631 19.27060152801813 34-35 19.608923348976397 29.198760457049584 32.097403591103856 19.094912602870163 36-37 19.24692061767455 28.890402171986324 31.62680908530963 20.23586812502949 38-39 20.086289447500167 29.500518323225428 30.805103241740937 19.608088987533463 40-41 21.33458802407884 27.499063869794384 32.12828617829784 19.03806192782893 42-43 19.490511104027203 29.433598979917118 31.910402720221022 19.165487195834665 44-45 19.202567640907667 29.745849619439547 30.893502146429817 20.15808059322297 46-47 20.718389961870784 28.48844499017974 30.58404302298251 20.209122024966963 48-49 20.06652417153988 29.00950302805686 30.38406106011104 20.53991174029222 50-51 19.03958269407794 30.22917996530384 30.560204471062026 20.1710328695562 52-53 19.292376434250055 29.285836011761713 30.974276463106108 20.447511090882124 54-55 19.68119105417406 28.619962193073718 30.90257236694478 20.796274385807443 56-57 20.190069178653296 29.307304004440216 29.139095926202636 21.363530890703846 58-59 20.102260986936248 29.79529528061848 28.913909440675496 21.18853429176978 60-61 19.606568189718992 28.04072862401712 31.85524415670295 20.497459029560932 62-63 20.052654505275015 28.084612687653387 31.17554689077059 20.68718591630101 64-65 20.587554053910477 29.05831978665307 29.4585790329264 20.895547126510056 66-67 20.35996494863411 30.207154932306835 28.925451252338384 20.507428866720666 68-69 20.775676833476695 28.09279664906656 31.06281470249246 20.068711814964285 70-71 21.378061986906282 28.063601945527278 30.21419670497993 20.34413936258651 72-73 20.45462562705041 29.515984871313083 29.417807360423243 20.611582141213262 74-75 19.95126105848673 29.760291787649855 30.512030885562936 19.77641626830048 76-77 20.26981024544547 28.563226231169146 30.954017170515602 20.21294635286978 78-79 19.969265369227802 28.376325834684064 30.61750599010814 21.03690280597999 80-81 20.143424439102837 29.17782964501729 30.31913085163892 20.35961506424096 82-83 19.924365933559663 28.92097778578678 30.969124164650726 20.185532116002836 84-85 19.89970634357377 28.784109405080127 30.828400103693443 20.48778414765266 86-87 19.039170407036686 30.18072765318377 29.37478805042084 21.405313889358705 88-89 19.88035379573864 28.70318815693842 30.363826468375578 21.052631578947366 90-91 20.084750373636165 28.644504105322042 31.042959232426348 20.227786288615444 92-93 19.59691290416768 29.42414246983817 30.68909694499235 20.289847681001795 94-95 19.64250572425234 29.55826666196287 30.115241621467 20.68398599231779 96-97 20.884580826743306 28.96036973849859 29.57091939044491 20.584130044313195 98-99 20.817941032968566 28.088646049909304 30.765163788809424 20.32824912831271 100-101 20.23703631859907 28.15122136108924 31.228116296768004 20.383626023543684 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 994.0 1 658.0 2 971.0 3 1333.0 4 774.0 5 587.5 6 921.5 7 1508.0 8 2030.0 9 2173.0 10 1856.0 11 1503.5 12 1529.5 13 1631.0 14 1727.0 15 1889.5 16 2021.5 17 2212.0 18 2423.0 19 2685.5 20 3025.5 21 3463.5 22 3987.0 23 4730.5 24 5609.0 25 6613.0 26 7978.0 27 9747.5 28 11620.0 29 14012.0 30 17312.5 31 21652.5 32 27868.5 33 36352.0 34 46954.0 35 57416.5 36 64474.0 37 65994.0 38 61647.5 39 53939.5 40 46991.0 41 42729.5 42 39871.0 43 37285.5 44 35465.5 45 34362.0 46 33066.5 47 32222.0 48 31892.5 49 30920.0 50 29416.5 51 27356.0 52 25257.0 53 23669.5 54 22197.0 55 20413.0 56 18042.0 57 15775.0 58 14011.0 59 12084.0 60 9914.5 61 8057.0 62 6566.0 63 5275.5 64 4236.0 65 3376.5 66 2605.5 67 2060.5 68 1587.0 69 1126.5 70 835.5 71 650.0 72 535.0 73 417.0 74 306.0 75 246.5 76 165.5 77 95.0 78 64.5 79 51.5 80 32.0 81 15.5 82 9.0 83 5.0 84 3.5 85 3.0 86 2.5 87 1.5 88 1.5 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3003561949102629 2 0.034251145033626466 3 0.042920169334941354 4 0.045639863233393085 5 0.05719856230181293 6 0.0575385240391194 7 0.031446460700848125 8 0.035016058942566014 9 0.039180590224570225 10-11 0.032338860261277594 12-13 0.03467609720525955 14-15 0.02821682419643669 16-17 0.021247608581654132 18-19 0.0257096063838015 20-21 0.03671586762909835 22-23 0.030469070706092026 24-25 0.05022934668703037 26-27 0.04262270281479819 28-29 0.03505855415972932 30-31 0.035695982417178945 32-33 0.03174392722099128 34-35 0.030851527660561807 36-37 0.02995912810013233 38-39 0.01814545772873263 40-41 0.018485419466039096 42-43 0.01937781902646857 44-45 0.009731404730397594 46-47 0.017720505557099548 48-49 0.01602069687056722 50-51 0.00883900516996812 52-53 0.006246796923006316 54-55 0.008414052998335036 56-57 0.00713919648343579 58-59 0.008796509952804812 60-61 0.00713919648343579 62-63 0.005566873448393383 64-65 0.00981639516472421 66-67 0.003739579110371128 68-69 0.00807409126102857 70-71 0.013683459926585264 72-73 0.013810945578075186 74-75 0.011133746896786766 76-77 0.00930645255876451 78-79 0.006799234746129323 80-81 0.00807409126102857 82-83 0.012663574714665863 84-85 0.004929445190943759 86-87 0.00220975129249203 88-89 0.018527914683202404 90-91 0.027621891156150373 92-93 0.013385993406442106 94-95 0.02112012293016421 96-97 0.017338048602629774 98-99 0.012663574714665863 100-101 0.10313589205534918 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1176603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.97694907702501 #Duplication Level Percentage of deduplicated Percentage of total 1 88.64626760980843 65.5778042483913 2 7.528721766566665 11.139037334807838 3 1.6929832548496337 3.757252080968022 4 0.6638397175856267 1.9643534793255424 5 0.36257436660363884 1.3411072727435993 6 0.22934696966567739 1.017983345755669 7 0.1515216409857504 0.7846376103489088 8 0.11858482168831405 0.7018034652275599 9 0.08940115647538044 0.5952262320005727 >10 0.4604498553622905 6.217232311491264 >50 0.03510086054595649 1.7957755287046109 >100 0.019133292879054013 2.76996065261997 >500 0.0010373434918112764 0.608714820123054 >1k 9.220831038322458E-4 1.30081990415987 >5k 1.1526038797903073E-4 0.4282917133321334 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5023 0.42690695162259484 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4056 0.34472120162875663 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2652 0.2253946318341871 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1950 0.16573134693690225 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1571 0.1335199723271146 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1418 0.12051643587514226 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1281 0.1088727463723958 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1267 0.10768288029182316 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.499043432661654E-5 0.0 10-11 0.0 0.0 0.0 8.499043432661654E-5 0.0 12-13 0.0 0.0 0.0 8.499043432661654E-5 0.0 14-15 0.0 0.0 0.0 1.6998086865323308E-4 0.0 16-17 0.0 0.0 0.0 1.6998086865323308E-4 0.0 18-19 0.0 0.0 0.0 1.6998086865323308E-4 0.0 20-21 0.0 0.0 0.0 2.9746652014315785E-4 0.0 22-23 0.0 0.0 0.0 6.374282574496241E-4 0.0 24-25 0.0 0.0 0.0 9.348947775927819E-4 0.0 26-27 0.0 0.0 0.0 0.0021247608581654136 0.0 28-29 0.0 0.0 0.0 0.0052269117110869175 0.0 30-31 0.0 0.0 0.0 0.013301002972115488 0.0 32-33 0.0 0.0 0.0 0.030511565923255338 0.0 34-35 0.0 0.0 0.0 0.057156067084649625 0.0 36-37 0.0 0.0 0.0 0.10360333944414556 0.0 38-39 0.0 0.0 0.0 0.17384793341509414 0.0 40-41 0.0 0.0 0.0 0.24982938170308933 0.0 42-43 0.0 0.0 0.0 0.33154768430813114 0.0 44-45 0.0 0.0 0.0 0.4302215785613329 0.0 46-47 0.0 0.0 0.0 0.5462760166343279 0.0 48-49 0.0 0.0 0.0 0.6749515342048252 0.0 50-51 0.0 0.0 0.0 0.8159081695355188 0.0 52-53 0.0 0.0 0.0 0.9530402353215146 0.0 54-55 0.0 0.0 0.0 1.105130617548995 0.0 56-57 0.0 0.0 0.0 1.290324773946692 0.0 58-59 0.0 0.0 0.0 1.4927294932955295 0.0 60-61 0.0 0.0 0.0 1.7205038572908617 0.0 62-63 0.0 0.0 0.0 1.9438587187012102 0.0 64-65 0.0 0.0 0.0 2.1758401091957102 0.0 66-67 0.0 0.0 0.0 2.44776700382372 0.0 68-69 0.0 0.0 0.0 2.765418752119449 0.0 70-71 0.0 0.0 0.0 3.0952666277410477 0.0 72-73 0.0 0.0 0.0 3.4063741125936273 0.0 74-75 0.0 4.249521716330827E-5 0.0 3.7254706982729093 0.0 76-77 0.0 8.499043432661654E-5 0.0 4.089102271539338 0.0 78-79 0.0 8.499043432661654E-5 0.0 4.489364722000539 0.0 80-81 0.0 8.499043432661654E-5 0.0 4.890689552890822 0.0 82-83 0.0 8.499043432661654E-5 0.0 5.29014459422592 0.0 84-85 0.0 8.499043432661654E-5 0.0 5.699033573771272 0.0 86-87 0.0 8.499043432661654E-5 0.0 6.122965860192435 0.0 88-89 0.0 8.499043432661654E-5 0.0 6.564873623473678 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1245 0.0 46.949642 2 GTACGAC 80 2.2543827E-7 41.581924 3 GTACCTG 1535 0.0 39.1316 1 ACCTGGG 1495 0.0 36.873413 3 GGTATCA 3490 0.0 34.69555 1 GTATCAA 5310 0.0 33.756607 1 TAGGACC 1425 0.0 33.34891 4 GTACATG 5175 0.0 29.202118 1 TACATGG 5085 0.0 28.877798 2 GTGTAGC 490 0.0 28.214174 1 ATCAACG 6420 0.0 28.05438 3 TCAACGC 6460 0.0 27.88067 4 TATCAAC 6445 0.0 27.724354 2 ACATGGG 5075 0.0 27.623743 3 CAACGCA 6530 0.0 27.436247 5 AACGCAG 6780 0.0 26.631464 6 GTAGCCC 395 0.0 24.061874 3 TAGTACG 120 1.7054376E-4 23.7611 4 ACGCAGA 7595 0.0 23.580006 7 CGCAGAG 7625 0.0 23.487234 8 >>END_MODULE