FastQCFastQC Report
Thu 26 May 2016
SRR938541_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938541_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1176603
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47580.4043844865260415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40950.34803582856749476No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28300.2405229291443248No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18620.15825218871616No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16580.14091414011353023No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13370.11363221069468632No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12360.10504817682769804No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12320.10470821509039159No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11780.10011873163675428No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG12450.043.1655542
GTACCTG14150.038.315691
GGTATCA34000.037.067661
ACCTGGG14100.036.4200443
GTATCAA53850.034.708441
TAGGACC14450.032.905464
GTACATG52400.031.3123781
TACATGG53700.030.0229682
ACATGGG50850.029.3612483
TAAGACG1303.0100273E-729.260554
AGGACCG2002.3646862E-1128.5290345
ATCAACG65500.027.8031063
TCAACGC65550.027.7818974
TATCAAC66500.027.7484252
CAACGCA66100.027.5507345
CATGGGG25750.027.5134374
GCGAGCG700.0083952227.140499
AACGCAG67650.026.8897486
ACAGCGC950.001227195824.9978188
CCGACCT950.001227195824.9978189