##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938541_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1176603 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.8251304815643 31.0 31.0 34.0 30.0 34.0 2 32.0657944948296 33.0 31.0 34.0 30.0 34.0 3 32.13085552221097 33.0 31.0 34.0 30.0 34.0 4 35.77935718334901 37.0 35.0 37.0 35.0 37.0 5 35.66594594778358 37.0 35.0 37.0 33.0 37.0 6 35.717637129941025 37.0 35.0 37.0 33.0 37.0 7 35.66237379982883 37.0 35.0 37.0 33.0 37.0 8 35.65239507293454 37.0 35.0 37.0 33.0 37.0 9 37.2190127001206 39.0 37.0 39.0 33.0 39.0 10-11 37.141561767223095 39.0 37.0 39.0 33.0 39.0 12-13 37.056587906031176 39.0 37.0 39.0 33.0 39.0 14-15 38.10473753679023 40.0 37.0 41.0 33.0 41.0 16-17 38.04667547167566 40.0 37.0 41.0 33.0 41.0 18-19 38.08052631176361 40.0 37.0 41.0 33.0 41.0 20-21 38.102659520670954 40.0 37.0 41.0 33.0 41.0 22-23 38.10491899136752 40.0 37.0 41.0 33.0 41.0 24-25 38.04495823995009 40.0 37.0 41.0 33.0 41.0 26-27 37.88826350094297 40.0 37.0 41.0 33.0 41.0 28-29 37.766860614837796 40.0 37.0 41.0 32.5 41.0 30-31 37.504968115838565 39.0 36.5 41.0 32.0 41.0 32-33 37.27796971450863 39.0 36.0 41.0 31.5 41.0 34-35 37.09328635062124 39.0 36.0 41.0 31.0 41.0 36-37 37.049699431329 39.0 36.0 41.0 31.0 41.0 38-39 36.89886648257739 39.0 36.0 41.0 31.0 41.0 40-41 36.70793844652784 39.0 35.0 41.0 30.0 41.0 42-43 36.52940584037266 39.0 35.0 41.0 30.0 41.0 44-45 36.258622916990696 39.0 35.0 40.5 30.0 41.0 46-47 35.86070662746908 39.0 35.0 40.0 28.5 41.0 48-49 35.4766603518774 38.0 35.0 40.0 27.0 41.0 50-51 35.177707773990036 38.0 34.5 40.0 26.5 41.0 52-53 34.7287980737768 38.0 34.0 40.0 24.5 41.0 54-55 34.473260734504336 38.0 33.5 40.0 24.0 41.0 56-57 34.128766457335225 38.0 33.0 40.0 23.0 41.0 58-59 33.71770087276677 37.5 33.0 40.0 22.5 41.0 60-61 33.385934763042414 37.0 32.0 40.0 22.0 41.0 62-63 32.95593670932337 36.5 31.5 39.0 20.0 41.0 64-65 32.502592633199136 36.0 31.0 39.0 18.5 40.0 66-67 32.02121828688181 35.5 31.0 39.0 16.5 40.0 68-69 31.45962019474708 35.0 30.0 38.0 13.0 40.0 70-71 30.937710935634193 35.0 29.0 38.0 8.0 40.0 72-73 30.37982480071868 34.0 29.0 37.0 7.5 39.0 74-75 29.78597793818306 34.0 28.0 36.5 6.5 39.0 76-77 28.4469523705107 32.5 27.0 35.0 2.0 37.5 78-79 29.104896468902425 34.0 28.0 36.0 2.0 38.0 80-81 28.774507204214167 34.0 28.0 35.5 2.0 37.5 82-83 28.301588131255826 33.5 26.5 35.0 2.0 37.0 84-85 27.706861192772756 33.0 26.0 35.0 2.0 36.5 86-87 27.043008559386642 32.5 25.5 35.0 2.0 36.0 88-89 26.266726754903736 32.0 24.5 35.0 2.0 35.5 90-91 25.704464462524744 32.0 22.0 34.0 2.0 35.0 92-93 24.977425690738507 31.0 19.5 34.0 2.0 35.0 94-95 24.16981046283241 31.0 12.0 34.0 2.0 35.0 96-97 23.064394702376248 30.5 2.0 34.0 2.0 35.0 98-99 21.86403570278165 30.0 2.0 34.0 2.0 35.0 100-101 18.825205698098678 24.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4376692300563825 1101 2 0.2985761414891783 1101 3 0.35998181253489037 1101 4 0.379934046721651 1101 5 0.2781560249190491 1101 6 0.3333713589027951 1101 7 0.42079620376952676 1101 8 0.3701283855523485 1101 9 0.5410703339023328 1101 10-11 0.5406525396396944 1101 12-13 0.5461828399987851 1101 14-15 0.6826497499139847 1101 16-17 0.6432950157007937 1101 18-19 0.7176367027893562 1101 20-21 0.767255259050863 1101 22-23 0.7607656692501195 1101 24-25 0.8120612262902895 1101 26-27 0.7276078194849518 1101 28-29 0.7046011720433611 1101 30-31 0.7905888280809563 1101 32-33 0.7170706077683136 1101 34-35 0.60546653471593 1101 36-37 0.7314890598547308 1101 38-39 0.83882363632123 1101 40-41 0.858030739539025 1101 42-43 0.9472383824238477 1101 44-45 0.9151647219771561 1101 46-47 1.0081764813395395 1101 48-49 1.1903846256650255 1101 50-51 1.1472695411232863 1101 52-53 1.3141002372069295 1101 54-55 1.439662274917687 1101 56-57 1.3975015372136212 1101 58-59 1.620805176580653 1101 60-61 1.308676048140832 1101 62-63 1.5638654110621175 1101 64-65 1.6473174613701147 1101 66-67 1.6536243350501252 1101 68-69 1.5319162874935195 1101 70-71 1.7934355829475948 1101 72-73 1.9132241749549443 1101 74-75 1.9268299088207428 1101 76-77 1.7981373163497345 1101 78-79 2.139740350398018 1101 80-81 2.503709236415638 1101 82-83 2.441876289861323 1101 84-85 2.5853263620416094 1101 86-87 2.641680080202505 1101 88-89 2.8567125601679244 1101 90-91 3.279136220468512 1101 92-93 3.438819763960826 1101 94-95 3.679959217103729 1101 96-97 4.132132102191331 1101 98-99 4.3090505354016315 1101 100-101 4.667911112827593 1102 1 0.3089834593053986 1102 2 0.2935794352968131 1102 3 0.3047117203082763 1102 4 0.21175881615511827 1102 5 0.219457078937495 1102 6 0.15924751173547236 1102 7 0.29183704698428414 1102 8 0.2576942617051827 1102 9 0.402368094113136 1102 10-11 0.37510247376354755 1102 12-13 0.3954318518564932 1102 14-15 0.5216714890444152 1102 16-17 0.41743203809868135 1102 18-19 0.48506917973270447 1102 20-21 0.4972865502234569 1102 22-23 0.534719885323895 1102 24-25 0.528540145921383 1102 26-27 0.5649777140831063 1102 28-29 0.5434055198694452 1102 30-31 0.4674086568029594 1102 32-33 0.3225465629725264 1102 34-35 0.485578524175736 1102 36-37 0.655171536798079 1102 38-39 0.7300390513409951 1102 40-41 0.6461664444138648 1102 42-43 0.8467146670088326 1102 44-45 0.8166238787623996 1102 46-47 0.6806665999166128 1102 48-49 0.8867284991828086 1102 50-51 0.9177669060771692 1102 52-53 0.9438334387879621 1102 54-55 0.8968625384223046 1102 56-57 1.0893493633005775 1102 58-59 0.9374454927861819 1102 60-61 1.0080963379959016 1102 62-63 1.2185673214705446 1102 64-65 1.1263079093279558 1102 66-67 1.2992122797141548 1102 68-69 1.2605625325528145 1102 70-71 1.3938786000753893 1102 72-73 1.5976872843093624 1102 74-75 1.5844122540117809 1102 76-77 1.5457180146369538 1102 78-79 1.9655852120580946 1102 80-81 2.1428446119097124 1102 82-83 2.0249477654871484 1102 84-85 2.2792558745580784 1102 86-87 2.190691937910014 1102 88-89 2.2365149317094293 1102 90-91 2.3487426104553357 1102 92-93 2.3705177218000877 1102 94-95 2.790357767828368 1102 96-97 3.0098280837460045 1102 98-99 3.1057303772988654 1102 100-101 3.6754654606536796 1103 1 0.3643358967230519 1103 2 0.3887493016623367 1103 3 0.453704756257828 1103 4 0.2802370770246867 1103 5 0.2761646829277069 1103 6 0.255276120807558 1103 7 0.3670299700032942 1103 8 0.2828989483229094 1103 9 0.4891222819542804 1103 10-11 0.42156163054878704 1103 12-13 0.4930226668386055 1103 14-15 0.6295978018620332 1103 16-17 0.6214551888609634 1103 18-19 0.6265111616638208 1103 20-21 0.7098959516915571 1103 22-23 0.631869565354009 1103 24-25 0.5724724817015456 1103 26-27 0.7189714558485818 1103 28-29 0.6890600465022345 1103 30-31 0.7371256246177538 1103 32-33 0.7904472311449311 1103 34-35 0.722111556360943 1103 36-37 0.7390648174304886 1103 38-39 0.953888571386166 1103 40-41 0.7411692676775559 1103 42-43 0.7063725815580497 1103 44-45 0.7278487046611417 1103 46-47 0.8669643601274188 1103 48-49 0.7898435001239008 1103 50-51 0.8997803636341075 1103 52-53 1.1116976398043406 1103 54-55 1.2023679026233154 1103 56-57 1.250964740676828 1103 58-59 1.4596363454118162 1103 60-61 1.4579834074481965 1103 62-63 1.492545064741769 1103 64-65 1.4057157297683816 1103 66-67 1.528429529855316 1103 68-69 1.642717153294388 1103 70-71 1.6732840677960752 1103 72-73 1.5135704953012628 1103 74-75 1.682977094967928 1103 76-77 1.4833321215445423 1103 78-79 1.853658099315762 1103 80-81 2.087973305679707 1103 82-83 2.2178719608569963 1103 84-85 2.506300388015635 1103 86-87 2.4700350585574817 1103 88-89 2.6856303090856777 1103 90-91 2.7340063503386425 1103 92-93 2.9667851319261906 1103 94-95 3.2251756673201797 1103 96-97 3.75760829266752 1103 98-99 3.9633578947089916 1103 100-101 4.399339684256166 1104 1 0.3443095462619148 1104 2 0.3668096471139819 1104 3 0.3127942175212084 1104 4 0.23949014279948955 1104 5 0.1717285393611263 1104 6 0.14726311931629965 1104 7 0.22689836248037665 1104 8 0.1861362906906905 1104 9 0.3595866002446897 1104 10-11 0.38227917387503396 1104 12-13 0.46859238697354755 1104 14-15 0.5544892147969236 1104 16-17 0.5304476456795157 1104 18-19 0.4729802722633636 1104 20-21 0.5155070073026096 1104 22-23 0.4126811450786363 1104 24-25 0.4912631113617394 1104 26-27 0.40231745767284366 1104 28-29 0.4675275934480396 1104 30-31 0.5978782777617155 1104 32-33 0.48980548159014603 1104 34-35 0.5514926824812036 1104 36-37 0.7472841343454562 1104 38-39 0.6570875463242771 1104 40-41 0.7070638803113027 1104 42-43 0.6817899178449522 1104 44-45 0.7939789512261655 1104 46-47 0.7534090748330087 1104 48-49 0.952782010888491 1104 50-51 0.8567997934796239 1104 52-53 1.0502297598581976 1104 54-55 1.1511824938292108 1104 56-57 1.3458292964109475 1104 58-59 1.5866929844249782 1104 60-61 1.5656492922879863 1104 62-63 1.6570776336221655 1104 64-65 1.6271091913792404 1104 66-67 1.6164154569716729 1104 68-69 1.9271595225193643 1104 70-71 1.8154574740999152 1104 72-73 2.0244570724921864 1104 74-75 1.8246575159961758 1104 76-77 1.752483622217781 1104 78-79 2.1958318675764907 1104 80-81 2.5388521369933237 1104 82-83 2.7303895157658538 1104 84-85 2.7946544252827152 1104 86-87 2.783501302458511 1104 88-89 2.9355115872278255 1104 90-91 3.1117373150261294 1104 92-93 3.2768373985001986 1104 94-95 3.394942494695698 1104 96-97 3.8168584627872555 1104 98-99 3.874125026128297 1104 100-101 4.315202082727257 1105 1 0.3568090959899557 1105 2 0.3312499382806209 1105 3 0.28045707049978574 1105 4 0.264937436041528 1105 5 0.1930298669014192 1105 6 0.21305200965221616 1105 7 0.22699188050372499 1105 8 0.24468965692389588 1105 9 0.3077194263177887 1105 10-11 0.3591906129996403 1105 12-13 0.523831405186705 1105 14-15 0.5450068335925167 1105 16-17 0.4731952190599884 1105 18-19 0.5502306622681559 1105 20-21 0.4931534288181254 1105 22-23 0.47817735863777955 1105 24-25 0.33375174372645944 1105 26-27 0.45623891084594703 1105 28-29 0.4689507992181774 1105 30-31 0.5606536017497135 1105 32-33 0.4768393808345195 1105 34-35 0.48706742347091847 1105 36-37 0.5077449911645147 1105 38-39 0.513739656506516 1105 40-41 0.4188001151509937 1105 42-43 0.5069597050117878 1105 44-45 0.43785885468786034 1105 46-47 0.4758468888111125 1105 48-49 0.5630459949502509 1105 50-51 0.48846936035955935 1105 52-53 0.5528375272573953 1105 54-55 0.42902696573639076 1105 56-57 0.6238064253660625 1105 58-59 0.8095142222988088 1105 60-61 0.8567539271797671 1105 62-63 0.9540119049989997 1105 64-65 1.112869167327787 1105 66-67 0.8693477665482021 1105 68-69 1.2365607854975913 1105 70-71 1.214134159433243 1105 72-73 1.2929379657209346 1105 74-75 1.4910153422770946 1105 76-77 1.398947740391307 1105 78-79 1.5503455322915123 1105 80-81 1.7296379100617933 1105 82-83 1.9352622204179255 1105 84-85 2.0720714851868998 1105 86-87 2.0651831305903947 1105 88-89 2.202465365771605 1105 90-91 2.5081054906349642 1105 92-93 2.720948256843254 1105 94-95 2.5989028790755597 1105 96-97 2.781359713850591 1105 98-99 3.3154085228988066 1105 100-101 3.709932653838365 1106 1 0.10519751887171935 1106 2 0.1133887040324737 1106 3 0.16110951632982307 1106 4 0.09163984304218076 1106 5 0.0037922346026775244 1106 6 0.08551976934079164 1106 7 0.06557951529288886 1106 8 0.02501638493653502 1106 9 0.09371447215188766 1106 10-11 0.2044651791593708 1106 12-13 0.10783475286153532 1106 14-15 0.10245923340254848 1106 16-17 0.04103614417435608 1106 18-19 0.15971583449591975 1106 20-21 0.15777561930025996 1106 22-23 0.021578924580730074 1106 24-25 -0.016916142657443345 1106 26-27 -0.10093463298214544 1106 28-29 0.13754782742015692 1106 30-31 0.18378026830880856 1106 32-33 0.08954770729875605 1106 34-35 0.06707418616693417 1106 36-37 0.04317022373742674 1106 38-39 0.132017385840129 1106 40-41 0.03452254155580903 1106 42-43 0.28461502779371983 1106 44-45 -0.08058387320411953 1106 46-47 -0.1385728066290497 1106 48-49 0.05455789946996248 1106 50-51 -0.15354409908916722 1106 52-53 0.1307305966865755 1106 54-55 0.2038825043072663 1106 56-57 0.15105784542699752 1106 58-59 0.49553922322832733 1106 60-61 0.291309409698826 1106 62-63 0.46840894119836207 1106 64-65 0.3513271603925219 1106 66-67 0.5019639547868664 1106 68-69 0.09583707881058601 1106 70-71 0.39250763282827705 1106 72-73 0.8375317725224924 1106 74-75 1.0573641248164307 1106 76-77 1.1192965804556536 1106 78-79 1.4418891457178518 1106 80-81 1.352786674642104 1106 82-83 1.33763912567073 1106 84-85 1.2145500553677557 1106 86-87 1.4672169903710817 1106 88-89 1.8294998744624387 1106 90-91 1.805275470718687 1106 92-93 1.5835399548622213 1106 94-95 1.9629355083823086 1106 96-97 2.5697031128910446 1106 98-99 2.9614210226620443 1106 100-101 3.484388674216248 1107 1 0.14161360804681422 1107 2 0.060932600819320726 1107 3 0.03635741062101516 1107 4 0.09597172614748928 1107 5 -0.032950434411098684 1107 6 -0.083363090857965 1107 7 0.14468077314751326 1107 8 0.0726852037341672 1107 9 0.10546925734730905 1107 10-11 0.07334093676888642 1107 12-13 0.11777524430500108 1107 14-15 0.30075830254555314 1107 16-17 0.28126451330845725 1107 18-19 0.31395099704773344 1107 20-21 0.26123701742406524 1107 22-23 0.09992535824532922 1107 24-25 0.1375456760510545 1107 26-27 0.26069543910217163 1107 28-29 0.08302222467493436 1107 30-31 0.13069050272211058 1107 32-33 0.19521055992142067 1107 34-35 0.07394141509870167 1107 36-37 0.3701553277973133 1107 38-39 0.36608739230209153 1107 40-41 0.26547541657252083 1107 42-43 0.421031743667875 1107 44-45 0.45761238944127314 1107 46-47 0.16972552440174127 1107 48-49 0.41583319025832566 1107 50-51 0.15240411050127278 1107 52-53 0.4818947946232015 1107 54-55 0.5060468442956747 1107 56-57 0.376695616160994 1107 58-59 0.4428087650973964 1107 60-61 0.4124096007724063 1107 62-63 0.7056716311578128 1107 64-65 0.586345571417958 1107 66-67 0.9288246939017952 1107 68-69 0.8035759764887338 1107 70-71 0.8931754154834763 1107 72-73 0.9878713041415494 1107 74-75 0.7657742868111725 1107 76-77 0.7414297804645642 1107 78-79 0.9043366422640453 1107 80-81 1.1949577412003265 1107 82-83 1.1031561941675427 1107 84-85 1.411681027113378 1107 86-87 1.736798202212075 1107 88-89 1.9243546056224723 1107 90-91 2.025450514726888 1107 92-93 2.341979153912977 1107 94-95 2.3929690855247827 1107 96-97 2.8400388007559236 1107 98-99 2.8752526885116794 1107 100-101 3.3038979549328573 1108 1 0.09378359955784887 1108 2 0.10013039368858756 1108 3 0.1352750676668535 1108 4 0.045828474874149094 1108 5 -0.030817582119077258 1108 6 -0.02642336250482913 1108 7 0.03693989878419046 1108 8 0.10766504244384123 1108 9 0.08263924885834228 1108 10-11 0.1335117771763521 1108 12-13 0.2614907578873442 1108 14-15 0.14014974991398077 1108 16-17 0.24341964913188008 1108 18-19 0.15521001657234734 1108 20-21 0.26274646139690105 1108 22-23 0.14995188625891842 1108 24-25 0.27274304447211506 1108 26-27 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-0.07351361118642075 2316 56-57 0.11177559920383828 2316 58-59 0.13343457002570602 2316 60-61 0.2764900775046186 2316 62-63 0.6388357506735645 2316 64-65 0.530369515352028 2316 66-67 0.629738824149932 2316 68-69 0.7518606742649929 2316 70-71 0.38030863648459245 2316 72-73 0.6256133192527358 2316 74-75 0.8106664800798242 2316 76-77 0.8736808429699323 2316 78-79 0.9424164589550372 2316 80-81 0.9519733621756856 2316 82-83 1.3165856180535265 2316 84-85 1.6190479992950557 2316 86-87 1.5824927748488058 2316 88-89 1.8893449196518368 2316 90-91 1.930548796473257 2316 92-93 1.7308084930131749 2316 94-95 2.374469820692635 2316 96-97 2.5988983783295474 2316 98-99 2.8592855939809 2316 100-101 3.3905538534474964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 6.0 4 12.0 5 43.0 6 97.0 7 228.0 8 534.0 9 903.0 10 1520.0 11 2622.0 12 4038.0 13 5751.0 14 6452.0 15 7978.0 16 10313.0 17 13442.0 18 12740.0 19 9824.0 20 9607.0 21 10357.0 22 11424.0 23 12888.0 24 14972.0 25 17680.0 26 20497.0 27 24491.0 28 29575.0 29 35547.0 30 41751.0 31 49881.0 32 59615.0 33 72955.0 34 91266.0 35 117201.0 36 148975.0 37 188610.0 38 132335.0 39 10468.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.869639861227505 20.890156569511188 15.47065192817962 22.76955164108169 2 14.484182994976516 21.287739907965133 43.10027583251422 21.12780126454413 3 18.699573941504287 26.85587868084199 30.849847739119273 23.594699638534454 4 12.190177995466609 18.41377252990176 38.202605296773854 31.19344417785778 5 11.554144820070256 38.226573912037374 34.61955657116411 15.599724696728265 6 28.358078298287527 37.109798292202214 20.10236247910298 14.429760930407282 7 26.677477449913013 33.08125170512059 22.781855902118217 17.459414942848184 8 24.682921937135976 37.988684373573754 20.825376103919506 16.503017585370767 9 25.434237376583262 18.22509376569667 22.674937935735333 33.66573092198473 10-11 23.52050776685084 26.90444440478224 30.191194481061157 19.38385334730576 12-13 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28.999345127275216 31.599882539376296 20.19192317973218 38-39 19.977126449678188 29.48711737165822 30.963230619107364 19.57252555955623 40-41 21.31271203980218 27.53484114359443 32.14505339270058 19.007393423902812 42-43 19.412758901027637 29.525543428141354 31.862616980108122 19.199080690722887 44-45 19.20339834417414 29.825688673800943 30.816107457845792 20.15480552417912 46-47 20.619996719369237 28.58695753792911 30.613129492275643 20.179916250426015 48-49 20.037004835105808 29.127454205029224 30.400908377762082 20.434632582102886 50-51 19.123017704357377 30.179720772427064 30.518535138870117 20.178726384345442 52-53 19.260447236663513 29.289318487204262 30.908046299389003 20.542187976743218 54-55 19.682339752660837 28.678237264395896 30.77308148967834 20.866341493264933 56-57 20.216419641969296 29.33483086478617 29.0382992394206 21.41045025382393 58-59 20.03343523686409 29.91093852386914 28.825823153604063 21.229803085662706 60-61 19.567347695016927 28.20730526779211 31.743077316648012 20.482269720542952 62-63 20.016904597387565 28.255112387100834 31.027925307006697 20.700057708504907 64-65 20.61425986505219 29.208407593725326 29.30210954757042 20.875222993652066 66-67 20.22610903191015 30.32388648483491 28.802426991999408 20.64757749125553 68-69 20.66517933027367 28.200917899031108 30.874808771035184 20.25909399966004 70-71 21.259549737677023 28.226130648995458 30.021766050231047 20.492553563096475 72-73 20.416699600459967 29.798283703169208 28.97277161455478 20.81224508181604 74-75 19.97997625367265 29.964185030974765 29.97451136874545 20.08132734660714 76-77 20.125182410719674 28.787364982071267 30.79794119171887 20.289511415490185 78-79 19.803663597662084 28.652442667577766 30.391176973031687 21.152716761728467 80-81 19.932806562621376 29.42058621302173 30.08614630423346 20.56046092012344 82-83 19.685414133756364 29.19523251213566 30.715764862065903 20.403588492042076 84-85 19.597899616419916 29.088808181908586 30.51884030132243 20.794451900349074 86-87 18.71387052948598 30.535970854622 29.04083812084895 21.709320495043066 88-89 19.39583699854581 29.12664679590312 29.967244686610524 21.510271518940545 90-91 19.73163192369834 29.09357781322853 30.658307503104304 20.516482759968827 92-93 19.127314140987043 29.97286667700722 30.168983020956613 20.730836161049126 94-95 19.090296387141628 30.116487889288063 29.615044326761026 21.17817139680929 96-97 20.245656351377654 29.519260107274924 29.218223988890053 21.01685955245737 98-99 20.115960948595234 28.76361015567698 30.33249107812916 20.78793781759863 100-101 19.212362123213275 29.12104770209605 30.57605257208519 21.090537602605487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 988.0 1 644.0 2 964.5 3 1311.5 4 737.5 5 568.0 6 879.0 7 1540.0 8 2078.0 9 2100.5 10 1762.5 11 1432.0 12 1380.5 13 1477.0 14 1673.0 15 1822.5 16 1954.0 17 2149.0 18 2340.5 19 2610.0 20 2954.5 21 3391.0 22 3997.5 23 4683.0 24 5438.0 25 6436.5 26 7808.0 27 9526.5 28 11427.0 29 13750.0 30 16908.5 31 21252.0 32 27709.5 33 36365.5 34 47173.5 35 58039.5 36 65687.5 37 67375.0 38 62458.0 39 54109.0 40 46915.5 41 42837.0 42 39745.0 43 37300.0 44 35814.5 45 34830.0 46 33639.0 47 32872.5 48 33016.0 49 31504.5 50 29282.0 51 27487.5 52 25474.5 53 23919.0 54 22603.0 55 20587.0 56 17867.0 57 15539.5 58 13653.0 59 11657.5 60 9529.5 61 7656.0 62 6196.0 63 4836.5 64 3789.5 65 2954.5 66 2131.0 67 1693.5 68 1318.0 69 909.5 70 660.5 71 500.5 72 400.0 73 330.0 74 250.5 75 204.0 76 134.5 77 64.5 78 36.0 79 26.5 80 15.5 81 7.0 82 4.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.499043432661654E-5 2 0.044789958890126914 3 3.3996173730646616E-4 4 0.0 5 0.09960878903079458 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 7.224186917762406E-4 26-27 0.011686184719909775 28-29 0.0 30-31 0.0 32-33 4.67447388796391E-4 34-35 0.015170792527301052 36-37 0.003272131721574737 38-39 0.011473708634093232 40-41 0.007181691700599097 42-43 0.005736854317046616 44-45 0.0025497130297984965 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.1247608581654134E-4 68-69 2.5497130297984965E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 7.224186917762406E-4 84-85 0.0047594643222905264 86-87 0.001572323035042406 88-89 0.0 90-91 8.499043432661653E-4 92-93 4.67447388796391E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.649139089395489E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1176603.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.45238803386074 #Duplication Level Percentage of deduplicated Percentage of total 1 87.87143737152915 63.66495477535123 2 7.9757283070160625 11.5572112430515 3 1.8020459400012674 3.9168759509944544 4 0.7520549322214098 2.179527030883377 5 0.3908467890551014 1.415889161120436 6 0.2465366272599152 1.07173004296768 7 0.17173340484970792 0.8709746700582999 8 0.12509500273330637 0.7250745343304307 9 0.09342204269864479 0.6091785079666308 >10 0.5105848006995631 6.767316112168031 >50 0.03715405128990513 1.8909674358201214 >100 0.021130316807525723 2.964493972972503 >500 0.001173902020250204 0.6489467957457934 >1k 0.0010565118182251836 1.716859766569408 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4758 0.4043844865260415 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4095 0.34803582856749476 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2830 0.2405229291443248 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1862 0.15825218871616 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1658 0.14091414011353023 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1337 0.11363221069468632 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1236 0.10504817682769804 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1232 0.10470821509039159 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1178 0.10011873163675428 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.499043432661654E-5 0.0 10-11 0.0 0.0 0.0 8.499043432661654E-5 0.0 12-13 0.0 0.0 0.0 8.499043432661654E-5 0.0 14-15 0.0 0.0 0.0 1.6998086865323308E-4 0.0 16-17 0.0 0.0 0.0 1.6998086865323308E-4 0.0 18-19 0.0 0.0 0.0 1.6998086865323308E-4 0.0 20-21 0.0 0.0 0.0 2.9746652014315785E-4 0.0 22-23 0.0 0.0 0.0 6.374282574496241E-4 0.0 24-25 0.0 0.0 0.0 9.348947775927819E-4 0.0 26-27 0.0 0.0 0.0 0.0020822656410021054 0.0 28-29 8.499043432661654E-5 0.0 0.0 0.005099426059596993 0.0 30-31 8.499043432661654E-5 0.0 0.0 0.013470983840768721 0.0 32-33 8.499043432661654E-5 0.0 0.0 0.03089402287772511 0.0 34-35 8.499043432661654E-5 0.0 0.0 0.05813345707940571 0.0 36-37 8.499043432661654E-5 0.0 0.0 0.10538813856500451 0.0 38-39 8.499043432661654E-5 8.499043432661654E-5 0.0 0.17648263687921925 0.0 40-41 8.499043432661654E-5 8.499043432661654E-5 0.0 0.2532714942933173 0.0 42-43 8.499043432661654E-5 8.499043432661654E-5 0.0 0.33630714863042166 0.0 44-45 8.499043432661654E-5 8.499043432661654E-5 0.0 0.43583094722688964 0.0 46-47 8.499043432661654E-5 8.499043432661654E-5 0.0 0.553202737031947 0.0 48-49 8.499043432661654E-5 8.499043432661654E-5 0.0 0.6823032067740775 0.0 50-51 8.499043432661654E-5 8.499043432661654E-5 0.0 0.8230048708017912 0.0 52-53 8.499043432661654E-5 8.499043432661654E-5 0.0 0.9609868409310531 0.0 54-55 8.499043432661654E-5 8.499043432661654E-5 0.0 1.114522060542086 0.0 56-57 8.499043432661654E-5 8.499043432661654E-5 0.0 1.2998437025912732 0.0 58-59 8.499043432661654E-5 8.499043432661654E-5 0.0 1.5034807832378467 0.0 60-61 8.499043432661654E-5 8.499043432661654E-5 0.0 1.7318925754906287 0.0 62-63 8.499043432661654E-5 8.499043432661654E-5 0.0 1.9564797981987128 0.0 64-65 8.499043432661654E-5 8.499043432661654E-5 0.0 2.189651054773785 0.0 66-67 8.499043432661654E-5 8.499043432661654E-5 0.0 2.4624278537450612 0.0 68-69 8.499043432661654E-5 8.499043432661654E-5 0.0 2.7830117720250587 0.0 70-71 8.499043432661654E-5 8.499043432661654E-5 0.0 3.117321645448805 0.0 72-73 8.499043432661654E-5 8.499043432661654E-5 0.0 3.433528556360981 0.0 74-75 8.499043432661654E-5 8.499043432661654E-5 0.0 3.7583619963573103 0.0 76-77 8.499043432661654E-5 8.499043432661654E-5 0.0 4.132362402611586 0.0 78-79 1.2748565148992482E-4 8.499043432661654E-5 0.0 4.543758599969573 0.0 80-81 2.549713029798496E-4 8.499043432661654E-5 0.0 4.9577895007916855 0.0 82-83 2.549713029798496E-4 8.499043432661654E-5 0.0 5.371395449442165 0.0 84-85 2.549713029798496E-4 8.499043432661654E-5 0.0 5.796347621075248 0.0 86-87 2.549713029798496E-4 8.499043432661654E-5 0.0 6.239062793482594 0.0 88-89 2.549713029798496E-4 8.499043432661654E-5 0.0 6.704215440552165 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1245 0.0 43.165554 2 GTACCTG 1415 0.0 38.31569 1 GGTATCA 3400 0.0 37.06766 1 ACCTGGG 1410 0.0 36.420044 3 GTATCAA 5385 0.0 34.70844 1 TAGGACC 1445 0.0 32.90546 4 GTACATG 5240 0.0 31.312378 1 TACATGG 5370 0.0 30.022968 2 ACATGGG 5085 0.0 29.361248 3 TAAGACG 130 3.0100273E-7 29.26055 4 AGGACCG 200 2.3646862E-11 28.529034 5 ATCAACG 6550 0.0 27.803106 3 TCAACGC 6555 0.0 27.781897 4 TATCAAC 6650 0.0 27.748425 2 CAACGCA 6610 0.0 27.550734 5 CATGGGG 2575 0.0 27.513437 4 GCGAGCG 70 0.00839522 27.14049 9 AACGCAG 6765 0.0 26.889748 6 ACAGCGC 95 0.0012271958 24.997818 8 CCGACCT 95 0.0012271958 24.997818 9 >>END_MODULE