FastQCFastQC Report
Thu 26 May 2016
SRR938540_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938540_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174989
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46510.39583349290929537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38250.32553496245496766No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24510.20859769751035967No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18320.15591635325947734No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA15110.12859694856717807No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC14010.11923515879723129No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12100.1029796874694146No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG13700.041.2873952
GGTATCA32050.039.1432761
GTACCTG15550.037.7315141
GTATCAA51250.037.5094451
ACCTGGG15800.033.693963
TCGACGT600.003937760431.6883643
TATCAAC62250.030.4666462
ATCAACG62250.030.390293
GTACATG51650.030.1999951
TCAACGC62750.030.1481324
CAACGCA64000.029.4850335
TTCGACG650.00582459329.25082
GACCGTC650.005840373729.234617
TACATGG53500.028.6083742
AACGCAG66550.028.4896056
TAGGACC13550.027.7127044
CGACGTC700.00836365127.1614574
ACATGGG52600.026.2965243
ACGCAGA74050.025.6617077
CGCAGAG74450.025.4600268
Produced by FastQC (version 0.11.5)