##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938540_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1174989 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.001743846112603 31.0 31.0 34.0 28.0 34.0 2 31.188323465155843 31.0 31.0 34.0 28.0 34.0 3 31.22356549720891 31.0 31.0 34.0 27.0 34.0 4 34.883844018965284 37.0 35.0 37.0 32.0 37.0 5 34.89345261955644 37.0 35.0 37.0 32.0 37.0 6 34.87978610863591 37.0 35.0 37.0 32.0 37.0 7 34.8080492668442 37.0 35.0 37.0 32.0 37.0 8 34.75918838389126 37.0 35.0 37.0 32.0 37.0 9 36.22587956142568 39.0 35.0 39.0 32.0 39.0 10-11 36.0216499899148 38.5 35.0 39.0 30.0 39.0 12-13 35.853907994032284 38.0 35.0 39.0 30.0 39.0 14-15 36.974802317298284 39.0 36.0 41.0 31.0 41.0 16-17 36.937567926167816 39.0 36.0 41.0 31.0 41.0 18-19 36.966485643695385 39.0 36.0 41.0 31.0 41.0 20-21 36.928391244513776 39.0 36.0 41.0 30.5 41.0 22-23 36.737815843382364 39.0 36.0 41.0 30.0 41.0 24-25 36.60500481281102 39.0 36.0 41.0 30.0 41.0 26-27 36.406753169604144 39.0 35.5 41.0 29.5 41.0 28-29 36.160471289518455 39.0 35.0 40.0 28.5 41.0 30-31 35.85395863280422 38.5 35.0 40.0 27.0 41.0 32-33 35.53655055494137 38.0 34.5 40.0 27.0 41.0 34-35 35.249695954600426 38.0 34.0 40.0 26.5 41.0 36-37 34.93973773371495 38.0 34.0 40.0 25.0 41.0 38-39 34.659013829065636 38.0 33.5 40.0 24.5 41.0 40-41 34.34442960742611 38.0 33.0 40.0 24.0 41.0 42-43 33.981862383392524 38.0 33.0 40.0 23.0 41.0 44-45 33.52423214174771 38.0 32.5 40.0 20.0 41.0 46-47 32.95331786084806 37.5 31.5 40.0 16.0 41.0 48-49 32.447191846051325 37.0 31.0 40.0 11.5 41.0 50-51 31.762373520092527 36.5 30.0 39.0 9.0 40.5 52-53 31.800299407058276 36.5 30.0 39.0 8.5 40.0 54-55 31.79722491019065 37.0 30.5 39.0 7.5 40.5 56-57 31.348509220086314 36.0 29.5 39.0 2.0 40.5 58-59 30.886121061558875 36.0 29.0 39.0 2.0 40.0 60-61 30.39847351762442 35.5 28.0 39.0 2.0 40.0 62-63 29.693923092045967 35.0 27.5 38.5 2.0 40.0 64-65 29.024909594898336 34.0 26.0 38.0 2.0 40.0 66-67 28.28223498262537 34.0 25.5 38.0 2.0 40.0 68-69 27.420395424978445 33.5 22.5 37.0 2.0 39.5 70-71 26.59535876506078 32.5 21.0 36.5 2.0 39.0 72-73 25.704263614382775 31.5 16.0 36.0 2.0 39.0 74-75 24.765328441372645 31.0 9.5 35.5 2.0 38.0 76-77 23.796539371857946 30.5 4.5 35.0 2.0 37.5 78-79 22.81872851575632 29.5 2.0 34.0 2.0 36.5 80-81 21.83628357371856 29.0 2.0 34.0 2.0 36.0 82-83 20.838618914730265 27.5 2.0 34.0 2.0 35.5 84-85 19.834781857532285 26.0 2.0 33.0 2.0 35.0 86-87 18.787231199611227 25.5 2.0 32.5 2.0 35.0 88-89 17.636045103400967 21.5 2.0 32.0 2.0 34.5 90-91 16.465205631712294 15.5 2.0 31.0 2.0 34.0 92-93 15.321233220055678 4.5 2.0 31.0 2.0 34.0 94-95 14.037494393564536 2.0 2.0 30.5 2.0 34.0 96-97 12.562523564050387 2.0 2.0 29.0 2.0 34.0 98-99 10.878634608494206 2.0 2.0 27.5 2.0 33.5 100-101 7.735294543182958 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5983586470345088 1101 2 0.62903378349327 1101 3 0.6850880584577226 1101 4 0.550714365143314 1101 5 0.6097193689950942 1101 6 0.4282251781103952 1101 7 0.527485236434579 1101 8 0.5036721186211821 1101 9 0.7852248999713254 1101 10-11 0.8254306518729138 1101 12-13 0.7750187718423334 1101 14-15 0.8348740222669448 1101 16-17 0.8953807261169899 1101 18-19 0.8513067693915062 1101 20-21 0.9109717012145495 1101 22-23 0.896581114829651 1101 24-25 1.0186072455995685 1101 26-27 0.9461950759557709 1101 28-29 1.0605266354781335 1101 30-31 1.1080829347333179 1101 32-33 1.3453577212214896 1101 34-35 1.4466182317482321 1101 36-37 1.6291932336001764 1101 38-39 1.5491469819332693 1101 40-41 1.5882973870485628 1101 42-43 1.6131656921696162 1101 44-45 1.6262950106392537 1101 46-47 1.722332039227787 1101 48-49 1.593349839593806 1101 50-51 1.5766563662102833 1101 52-53 1.5608867974838319 1101 54-55 1.7082671847588564 1101 56-57 1.9836258446677846 1101 58-59 1.9245684854583196 1101 60-61 1.934132527921843 1101 62-63 2.0880836503431937 1101 64-65 2.0643449978475523 1101 66-67 2.207909869903613 1101 68-69 2.3854793976634134 1101 70-71 2.836561028808955 1101 72-73 2.8860184189873586 1101 74-75 3.315150671315525 1101 76-77 3.4228328155221597 1101 78-79 3.5401044175480223 1101 80-81 4.036752919943179 1101 82-83 4.221037605683911 1101 84-85 4.10350848595759 1101 86-87 4.074160365334173 1101 88-89 4.104372085625684 1101 90-91 4.076456018871607 1101 92-93 4.2138358055715415 1101 94-95 4.026886559996749 1101 96-97 3.7009536112788712 1101 98-99 3.2443697326072716 1101 100-101 2.4434737780525824 1102 1 0.6416774270727856 1102 2 0.5152815091315652 1102 3 0.6125238359848701 1102 4 0.4461074809500829 1102 5 0.5641087397190034 1102 6 0.576590192041472 1102 7 0.5253560801982076 1102 8 0.624516078741749 1102 9 0.7796964817481324 1102 10-11 0.7010781818080289 1102 12-13 0.7257172907072515 1102 14-15 0.9463399490865356 1102 16-17 0.9210873992808999 1102 18-19 0.7977854876535915 1102 20-21 0.9144373765280562 1102 22-23 0.7860579428663002 1102 24-25 1.0251699826145781 1102 26-27 0.9240275650408023 1102 28-29 0.9746993965827215 1102 30-31 0.9240877605064028 1102 32-33 0.9560573404255521 1102 34-35 1.0778262076350842 1102 36-37 1.1960474818917035 1102 38-39 1.3423795032425119 1102 40-41 1.3124258595027456 1102 42-43 1.576342449863155 1102 44-45 1.3048101029694905 1102 46-47 1.5387530289828462 1102 48-49 1.6016314635371813 1102 50-51 1.8215509890886956 1102 52-53 1.7757154393116465 1102 54-55 1.7555133895712665 1102 56-57 1.9116903874179272 1102 58-59 1.9952526999144204 1102 60-61 2.139495895273992 1102 62-63 2.2312566354453836 1102 64-65 2.3831073521017387 1102 66-67 2.2470622943830136 1102 68-69 2.411687904070927 1102 70-71 2.44482103894779 1102 72-73 2.777704177848033 1102 74-75 2.684848903306314 1102 76-77 2.8712563832146323 1102 78-79 3.4298433620869986 1102 80-81 3.579353728692446 1102 82-83 3.669303589092369 1102 84-85 3.7728636479235824 1102 86-87 3.6997943375191724 1102 88-89 3.7235093411440836 1102 90-91 3.75377252749551 1102 92-93 3.799798621968165 1102 94-95 3.821888269452197 1102 96-97 3.5108484109974984 1102 98-99 3.3272472766503096 1102 100-101 2.5093463666397087 1103 1 0.6416251822613965 1103 2 0.6592939418371309 1103 3 0.597556867182039 1103 4 0.41812063728526994 1103 5 0.49222221856842907 1103 6 0.4004495396519374 1103 7 0.4488439438065015 1103 8 0.5122139072290466 1103 9 0.6655805575296228 1103 10-11 0.7795760109191505 1103 12-13 0.7368945612511553 1103 14-15 0.7933168459630764 1103 16-17 0.6661190736725118 1103 18-19 0.9539526367400128 1103 20-21 0.8407628351907093 1103 22-23 0.728721991574055 1103 24-25 0.9474776593268075 1103 26-27 1.118844177955097 1103 28-29 1.0052013990405655 1103 30-31 0.9078973273891222 1103 32-33 1.2397852650433023 1103 34-35 1.0719143563284987 1103 36-37 1.130848296598856 1103 38-39 1.229003194003134 1103 40-41 1.394507916607104 1103 42-43 1.5639654980905746 1103 44-45 1.6224838988435621 1103 46-47 1.623189221692897 1103 48-49 1.5892568510845209 1103 50-51 1.438623033903827 1103 52-53 1.7356241746441157 1103 54-55 1.810560706215373 1103 56-57 1.6953364358387262 1103 58-59 1.9794427961696321 1103 60-61 2.221538183939831 1103 62-63 2.4239682503247053 1103 64-65 2.3680729329080847 1103 66-67 2.8846700590536685 1103 68-69 2.8127759997397526 1103 70-71 2.722972414779811 1103 72-73 2.9355178182664936 1103 74-75 2.97749548509206 1103 76-77 3.583038523910691 1103 78-79 3.7434900187309808 1103 80-81 3.438207742653507 1103 82-83 3.5217334406859138 1103 84-85 3.9401016284978674 1103 86-87 3.89121698712648 1103 88-89 4.312009712316176 1103 90-91 4.05254578813318 1103 92-93 4.075800162800213 1103 94-95 3.7616347963419035 1103 96-97 3.558750274967606 1103 98-99 3.0671624685057335 1103 100-101 2.499568399290376 1104 1 0.3366085435233366 1104 2 0.19572966953026594 1104 3 0.13082694173069243 1104 4 0.18335973772759218 1104 5 0.07917368792887203 1104 6 0.17573997741062186 1104 7 0.10990885264871508 1104 8 0.1307774985911152 1104 9 0.11600259527173051 1104 10-11 0.2470079756521315 1104 12-13 0.2820515634361058 1104 14-15 0.37044605671844977 1104 16-17 0.411312121522208 1104 18-19 0.29063653920503185 1104 20-21 0.43348827232766496 1104 22-23 0.4209090382276557 1104 24-25 0.5144089816031752 1104 26-27 0.45273250596916625 1104 28-29 0.41136535574431576 1104 30-31 0.41925356217888066 1104 32-33 0.4772473689850898 1104 34-35 0.7446785629093853 1104 36-37 0.9902428182499392 1104 38-39 0.7888452888261739 1104 40-41 0.8314080190448934 1104 42-43 0.8671772630402685 1104 44-45 0.8524163816867087 1104 46-47 0.9520063613694774 1104 48-49 1.1442116866807552 1104 50-51 0.9455183401217404 1104 52-53 0.9132604672253635 1104 54-55 1.2172609069460556 1104 56-57 1.4062957242218062 1104 58-59 1.660028615938355 1104 60-61 1.711151555327124 1104 62-63 1.6791167851091444 1104 64-65 1.560758475561876 1104 66-67 1.9358079030048074 1104 68-69 2.2092686899454 1104 70-71 2.2937077759470093 1104 72-73 2.422932182056808 1104 74-75 2.4650926677569807 1104 76-77 2.3484763566114353 1104 78-79 2.6819770186066307 1104 80-81 3.0067085686691506 1104 82-83 3.3564988552036574 1104 84-85 3.46924162482669 1104 86-87 3.583266364506084 1104 88-89 3.7008743404907136 1104 90-91 3.2989367807138414 1104 92-93 3.3700134643166493 1104 94-95 3.44027363210394 1104 96-97 3.5556145981972964 1104 98-99 3.166671448095153 1104 100-101 2.373751635613748 1105 1 0.5445008958633188 1105 2 0.4590198836749231 1105 3 0.3303720486616619 1105 4 0.3855970256715082 1105 5 0.4912576155587516 1105 6 0.27594862320611213 1105 7 0.37159252243989016 1105 8 0.2502519543238577 1105 9 0.5213585441506794 1105 10-11 0.7104857731102143 1105 12-13 0.5660883224148705 1105 14-15 0.8542480891772897 1105 16-17 0.7998654399940079 1105 18-19 0.8467058559748679 1105 20-21 0.9182900658898916 1105 22-23 0.742811616473368 1105 24-25 0.8657644017996944 1105 26-27 0.8001800582684382 1105 28-29 0.6794377428303306 1105 30-31 0.8025016796311988 1105 32-33 0.8074410024611467 1105 34-35 1.1238898224316785 1105 36-37 0.9541128867666089 1105 38-39 0.9475514445839366 1105 40-41 0.9627831305417516 1105 42-43 1.1181780843357103 1105 44-45 1.2523222366326507 1105 46-47 1.5216344742700372 1105 48-49 1.2579770082228734 1105 50-51 1.287949417036554 1105 52-53 1.439264857834047 1105 54-55 1.622846917499917 1105 56-57 1.6613206811426693 1105 58-59 1.4737617076421365 1105 60-61 1.5956888663125532 1105 62-63 1.8850484889508579 1105 64-65 2.2398485904725547 1105 66-67 2.2692584464592542 1105 68-69 2.628209939756328 1105 70-71 2.552656503645867 1105 72-73 2.6221546372071494 1105 74-75 2.8068512295145815 1105 76-77 2.8000676341993973 1105 78-79 3.0861911406474576 1105 80-81 3.269098679763143 1105 82-83 3.34330158456207 1105 84-85 3.4865952017026274 1105 86-87 3.542014645227976 1105 88-89 3.4935725151227217 1105 90-91 3.303106162650149 1105 92-93 3.5991534789331645 1105 94-95 3.712499144848154 1105 96-97 3.4010668690579884 1105 98-99 2.7088306917682807 1105 100-101 2.5112581911345915 1106 1 0.42888021118309183 1106 2 0.3602097955307606 1106 3 0.21239902091308238 1106 4 0.25707211790341944 1106 5 0.34119269253890394 1106 6 0.0507193315883967 1106 7 0.38068586731509413 1106 8 0.06350243351757712 1106 9 0.41495967635318465 1106 10-11 0.3609938215036337 1106 12-13 0.6615182914304469 1106 14-15 0.6627832334850154 1106 16-17 0.05161973556206334 1106 18-19 0.5143748791066969 1106 20-21 0.5533113977392716 1106 22-23 0.49480087154181973 1106 24-25 0.6681930036532648 1106 26-27 0.6181517119848507 1106 28-29 0.6502965816357502 1106 30-31 0.5342844176650985 1106 32-33 0.7703699581280006 1106 34-35 0.4764400261319537 1106 36-37 0.7395701303206863 1106 38-39 0.9195119136785195 1106 40-41 0.9098234652559896 1106 42-43 0.3576282653644469 1106 44-45 0.7920661170368888 1106 46-47 0.9983676440935412 1106 48-49 0.7516863454584595 1106 50-51 0.7014564967372898 1106 52-53 0.8484921708454465 1106 54-55 0.811733401832516 1106 56-57 1.2651762154007287 1106 58-59 1.5301557663270522 1106 60-61 1.9464201949329514 1106 62-63 1.767522021656255 1106 64-65 2.010634949081215 1106 66-67 2.2822667524836966 1106 68-69 2.281518265704239 1106 70-71 2.1446881476066544 1106 72-73 2.1816457643944354 1106 74-75 2.465383625757976 1106 76-77 2.4900505962005397 1106 78-79 3.0934972131826157 1106 80-81 2.913035075814175 1106 82-83 3.151872797214157 1106 84-85 3.129389090007553 1106 86-87 2.666526348546949 1106 88-89 3.0751993005057656 1106 90-91 2.9281465248450296 1106 92-93 3.0617990404657576 1106 94-95 2.5458311041493236 1106 96-97 2.5339715043555007 1106 98-99 2.7242957673056996 1106 100-101 2.0194024232910666 1107 1 0.40265293403717806 1107 2 0.430816710407143 1107 3 0.17324928500044123 1107 4 0.2394479320377627 1107 5 0.4252696829151077 1107 6 0.2765480474433559 1107 7 0.4837965895859213 1107 8 0.31436274264559927 1107 9 0.46703835170395536 1107 10-11 0.4503395977260496 1107 12-13 0.45280502873642803 1107 14-15 0.5473770574051784 1107 16-17 0.6248059576546794 1107 18-19 0.6614666778170388 1107 20-21 0.610354267380572 1107 22-23 0.4812562683209336 1107 24-25 0.6890137806857624 1107 26-27 0.6211230998206361 1107 28-29 0.4775494250303254 1107 30-31 0.7441577205392775 1107 32-33 0.8365991794753285 1107 34-35 0.7281784662343398 1107 36-37 0.7295088879759817 1107 38-39 0.9600387922631413 1107 40-41 0.9359924163593192 1107 42-43 1.0133425973692383 1107 44-45 0.8740125866911441 1107 46-47 0.9319456227721332 1107 48-49 0.8152115066651575 1107 50-51 0.9017335454395372 1107 52-53 1.243952460531446 1107 54-55 1.1845324038091185 1107 56-57 1.3276839111980152 1107 58-59 1.3191694081403362 1107 60-61 1.526164856479891 1107 62-63 1.7009023398572225 1107 64-65 1.6544799314213847 1107 66-67 1.9021656540098952 1107 68-69 1.8688728556397578 1107 70-71 2.041930621926994 1107 72-73 2.195396128932206 1107 74-75 2.4740774464378 1107 76-77 2.627265851701008 1107 78-79 2.6886059264453124 1107 80-81 3.0713708393992825 1107 82-83 3.2477624313802593 1107 84-85 2.8318063804388416 1107 86-87 2.9203742471892475 1107 88-89 2.5729245848452216 1107 90-91 2.6726768381854917 1107 92-93 2.804236096944809 1107 94-95 2.5830123361249537 1107 96-97 2.4885841222227114 1107 98-99 2.377144022384927 1107 100-101 1.6868467705254409 1108 1 0.4086682067508072 1108 2 0.2940752645652367 1108 3 0.14093267326634518 1108 4 0.16723401398006388 1108 5 0.07290216213558409 1108 6 0.31365974088834747 1108 7 0.3028149331031429 1108 8 0.4792880364358254 1108 9 0.4824036272319603 1108 10-11 0.28512035735904817 1108 12-13 0.3509919412341631 1108 14-15 0.37934187137452113 1108 16-17 0.4813712857178203 1108 18-19 0.40416460705721846 1108 20-21 0.5524818151781545 1108 22-23 0.4524169820482413 1108 24-25 0.5313017482640774 1108 26-27 0.5171341822319917 1108 28-29 0.701360035876327 1108 30-31 0.40055230942058984 1108 32-33 0.510869472733944 1108 34-35 0.4347928896299891 1108 36-37 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-0.061990874768897974 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4074.0 3 1542.0 4 1664.0 5 1879.0 6 2348.0 7 2924.0 8 3712.0 9 4863.0 10 6435.0 11 8238.0 12 10616.0 13 13052.0 14 13973.0 15 15797.0 16 18547.0 17 21551.0 18 21129.0 19 19808.0 20 21296.0 21 23393.0 22 26120.0 23 29086.0 24 32030.0 25 35460.0 26 38780.0 27 42601.0 28 47257.0 29 52178.0 30 57862.0 31 64284.0 32 71299.0 33 80121.0 34 90317.0 35 97748.0 36 98820.0 37 74102.0 38 19775.0 39 308.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.43275851167274 20.01702078210152 14.817650345969613 22.732570360256133 2 14.175226575815 20.68586538084998 43.979970408509 21.15893763482602 3 18.66526248038666 26.732551041518565 30.3931693205163 24.209017157578476 4 11.834493844946111 17.807339746373295 38.371469294548575 31.986697114132017 5 11.752993646086777 37.96269407283198 34.63542709010191 15.648885190979334 6 28.4018992731261 36.6582534589524 20.39342816074336 14.546419107178135 7 26.789287009588158 32.96165247996295 22.77259670314514 17.476463807303755 8 24.564859811811022 37.764651394049196 21.00490054181807 16.66558825232171 9 26.13347000385663 18.120195709010975 22.545953902644385 33.20038038448801 10-11 23.633944158592357 26.63937130419643 30.306749301971763 19.419935235239453 12-13 26.023722010220084 24.7686220759845 29.563635047961196 19.644020865834218 14-15 20.96389255065978 29.06405136061096 27.063412034567875 22.908644054161385 16-17 19.621232918983683 30.32877697688623 30.361422706871565 19.688567397258524 18-19 20.457491162392763 27.57903832361072 32.85627635054175 19.107194163454764 20-21 20.874963231741553 27.80612581534012 31.308592428058724 20.0103185248596 22-23 20.08035906599932 29.29606854401019 31.554874285184013 19.068698104806476 24-25 20.548240871450197 27.939075454958783 32.6880243711803 18.824659302410723 26-27 19.102038105827575 29.336966432850804 32.09129120334148 19.469704257980148 28-29 19.117941269866947 29.614081746801325 30.756188985950434 20.511787997381294 30-31 19.79105076105182 28.05600925938933 31.994314619167604 20.158625360391248 32-33 19.083092482473578 28.85021559521732 32.770260204059916 19.29643171824919 34-35 19.60471993226835 29.167516220989402 32.10370365956077 19.12406018718147 36-37 19.267951832461623 28.80249036168221 31.715333236563502 20.214224569292664 38-39 20.03963998011617 29.521656009751247 30.809253130681704 19.62945087945088 40-41 21.36848166509838 27.45283544828508 32.148373046930686 19.03030983968585 42-43 19.43394772983691 29.39518921111285 31.908598500985452 19.26226455806479 44-45 19.220761423566543 29.612921457341006 30.9147450061795 20.251572112912946 46-47 20.75425522542235 28.384910469061165 30.60659546639892 20.25423883911756 48-49 20.087428428667433 28.941652951443643 30.363226773817775 20.60769184607115 50-51 19.17631337033128 30.124314800313236 30.45695584079534 20.242415988560143 52-53 19.262154152047977 29.198095782838212 30.99315155941188 20.546598505701926 54-55 19.66615498603482 28.513179783749326 30.964310169535526 20.85635506068033 56-57 20.186070937789527 29.22814309102802 29.163540446234897 21.42224552494756 58-59 20.120619541182737 29.755373493319624 28.939923433997144 21.18408353150049 60-61 19.59912060002111 27.99793512935657 31.83628793114601 20.566656339476307 62-63 20.160531814835146 28.04174167656159 31.09461248079534 20.703114027807924 64-65 20.61624544957013 29.018315772809178 29.401458350533538 20.963980427087154 66-67 20.384887247577858 30.121074113051588 28.92075903353486 20.573279605835694 68-69 20.836592450989823 28.022638349012563 30.92831019253406 20.212459007463558 70-71 21.38267161995895 27.990073554602894 30.145811158356256 20.4814436670819 72-73 20.47435035365615 29.394826661673207 29.342693234145056 20.78812975052559 74-75 19.969570907233823 29.57605677260105 30.495143048654626 19.959229271510495 76-77 20.298538966842052 28.38256497027037 30.882092598487386 20.436803464400192 78-79 20.00413661203814 28.184499706138826 30.508662840158674 21.302700841664365 80-81 20.157143598879514 28.967084280618188 30.182900100353322 20.69287202014897 82-83 20.037775726414537 28.64813885601175 30.75270135172921 20.561384065844504 84-85 19.910867758377744 28.57836542942343 30.563413308638687 20.94735350356014 86-87 19.120539525208965 29.89572402100839 29.186890776614334 21.796845677168307 88-89 19.94290996594346 28.40201530288702 30.177832859923853 21.477241871245674 90-91 20.16888667932813 28.378521026344757 30.742330123700405 20.71026217062671 92-93 19.651110335611598 29.0181466885698 30.405534229318988 20.925208746499614 94-95 19.76635949584317 29.088570495852963 29.72461882428952 21.42045118401435 96-97 20.979011752448233 28.489291367336683 29.083829988087096 21.447866892127994 98-99 20.85764455441415 27.608269517707935 30.200218066865325 21.33386786101259 100-101 20.49679040624177 27.452529285998533 30.744738234685087 21.305942073074604 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 788.0 1 566.5 2 861.0 3 1176.5 4 734.0 5 579.0 6 849.0 7 1362.0 8 1839.5 9 1930.5 10 1626.5 11 1314.0 12 1323.5 13 1439.0 14 1537.5 15 1693.5 16 1936.0 17 2118.0 18 2338.5 19 2631.0 20 2923.0 21 3346.0 22 3907.5 23 4642.5 24 5407.0 25 6418.0 26 7797.5 27 9540.0 28 11609.0 29 13871.0 30 16916.0 31 21409.5 32 27555.5 33 35847.5 34 45870.5 35 55713.5 36 62965.0 37 64847.0 38 61256.5 39 54571.0 40 48126.5 41 43818.0 42 40578.0 43 37749.5 44 35782.5 45 34503.0 46 33187.5 47 32415.5 48 32119.0 49 31003.5 50 29380.0 51 27398.5 52 25541.0 53 24102.0 54 22543.5 55 20566.5 56 18196.5 57 16158.0 58 14352.0 59 12310.5 60 10189.0 61 8394.5 62 6965.5 63 5636.5 64 4379.5 65 3482.5 66 2761.0 67 2154.0 68 1658.5 69 1235.0 70 917.0 71 673.5 72 545.5 73 434.5 74 313.0 75 244.0 76 183.0 77 121.5 78 83.0 79 43.5 80 25.0 81 19.0 82 10.0 83 5.0 84 2.0 85 2.5 86 2.5 87 3.5 88 5.0 89 3.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3961739216282025 2 0.02783004777065998 3 0.034553514969076306 4 0.04314934012148199 5 0.06400059915454527 6 0.054894130923778865 7 0.03531947958661741 8 0.036170551383885294 9 0.0330215857339941 10-11 0.03663864087238263 12-13 0.029064101876698417 14-15 0.026766208024075117 16-17 0.02182999159992136 18-19 0.027361958282162643 20-21 0.03425563984003255 22-23 0.03285137137454053 24-25 0.04144719652694621 26-27 0.03557480112579778 28-29 0.03080879906109759 30-31 0.0338301039413986 32-33 0.028425798028747506 34-35 0.03008538803341989 36-37 0.03238328188604319 38-39 0.014042684654920172 40-41 0.011872451571887056 42-43 0.012808630548881735 44-45 0.011659683622570083 46-47 0.019361883387844482 48-49 0.01697888235549439 50-51 0.012680969779291552 52-53 0.01046818310639504 54-55 0.008723485921995865 56-57 0.009744772078717334 58-59 0.004680894884973391 60-61 0.008255396433498525 62-63 0.012255433880657605 64-65 0.008510717972678893 66-67 0.004042591037022474 68-69 0.006808574378143115 70-71 0.012553309009701367 72-73 0.010127754387487883 74-75 0.0107660582354388 76-77 0.005702181041694858 78-79 0.009744772078717334 80-81 0.011915005161750451 82-83 0.013659702346149626 84-85 0.008978807461176233 86-87 0.0025532153918036682 88-89 0.01514907799136843 90-91 0.025191725199129524 92-93 0.010127754387487883 94-95 0.020042740825658795 96-97 0.018085275691942647 98-99 0.010212861567214673 100-101 0.12561819727674048 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1174989.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.38004616279966 #Duplication Level Percentage of deduplicated Percentage of total 1 88.90996024716607 66.13126947516895 2 7.287947497832377 10.84155742641665 3 1.6679155494019315 3.7217890668050106 4 0.6760497989626584 2.011384610207758 5 0.3649834963614094 1.3573744654010826 6 0.23143039723034325 1.0328282177680796 7 0.15218791206492624 0.7923820747366516 8 0.11183203081823484 0.6654457291791953 9 0.08643985683548562 0.5786460487556292 >10 0.45702746762374946 6.223176195399961 >50 0.032536281952705165 1.6478251222468776 >100 0.019509051255999717 2.733153762376712 >500 0.0010328269708650111 0.5499581417770582 >1k 0.0011475855231833456 1.7132096637602567 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4651 0.39583349290929537 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3825 0.32553496245496766 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2451 0.20859769751035967 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1832 0.15591635325947734 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1511 0.12859694856717807 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1401 0.11923515879723129 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1210 0.1029796874694146 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.255358986339447E-5 0.0 12-13 0.0 0.0 0.0 8.510717972678894E-5 0.0 14-15 0.0 0.0 0.0 8.510717972678894E-5 0.0 16-17 0.0 0.0 0.0 8.510717972678894E-5 0.0 18-19 0.0 0.0 0.0 1.276607695901834E-4 0.0 20-21 0.0 0.0 0.0 3.8298230877055017E-4 0.0 22-23 0.0 0.0 0.0 7.659646175411003E-4 0.0 24-25 0.0 0.0 0.0 0.0014468220553554118 0.0 26-27 0.0 0.0 0.0 0.0028936441107108237 0.0 28-29 0.0 0.0 0.0 0.005744734631558253 0.0 30-31 0.0 0.0 0.0 0.014595881323144301 0.0 32-33 0.0 0.0 0.0 0.03246838906576997 0.0 34-35 0.0 0.0 0.0 0.06161759812219518 0.0 36-37 0.0 0.0 0.0 0.10719249286589067 0.0 38-39 0.0 0.0 0.0 0.17587398690540934 0.0 40-41 0.0 0.0 0.0 0.2560449502080445 0.0 42-43 0.0 0.0 0.0 0.3346414306857341 0.0 44-45 0.0 0.0 0.0 0.43485513481402804 0.0 46-47 0.0 0.0 0.0 0.5497072738553297 0.0 48-49 0.0 0.0 0.0 0.6786020975515515 0.0 50-51 0.0 0.0 0.0 0.8194119264095239 0.0 52-53 0.0 0.0 0.0 0.9542216990967576 4.255358986339447E-5 54-55 0.0 0.0 0.0 1.1132019108263993 8.510717972678894E-5 56-57 0.0 0.0 0.0 1.3027356000779582 8.510717972678894E-5 58-59 0.0 0.0 0.0 1.5032906691041363 8.510717972678894E-5 60-61 0.0 0.0 0.0 1.7299736423064387 8.510717972678894E-5 62-63 0.0 0.0 0.0 1.9555076685824293 8.510717972678894E-5 64-65 0.0 0.0 0.0 2.186956643849432 8.510717972678894E-5 66-67 0.0 0.0 0.0 2.4577251361502106 8.510717972678894E-5 68-69 0.0 0.0 0.0 2.7783238821810246 8.510717972678894E-5 70-71 0.0 0.0 0.0 3.106199292078479 8.510717972678894E-5 72-73 0.0 0.0 0.0 3.4155638903853567 8.510717972678894E-5 74-75 0.0 0.0 0.0 3.7335668674345035 8.510717972678894E-5 76-77 0.0 0.0 0.0 4.083102054572426 8.510717972678894E-5 78-79 0.0 0.0 0.0 4.466595006421336 8.510717972678894E-5 80-81 0.0 0.0 0.0 4.855407157003173 1.276607695901834E-4 82-83 0.0 0.0 0.0 5.243283128608012 1.7021435945357787E-4 84-85 0.0 0.0 0.0 5.625925008659656 1.7021435945357787E-4 86-87 0.0 0.0 0.0 6.018013785660973 1.7021435945357787E-4 88-89 0.0 0.0 0.0 6.43167723272303 1.7021435945357787E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 1370 0.0 41.287395 2 GGTATCA 3205 0.0 39.143276 1 GTACCTG 1555 0.0 37.731514 1 GTATCAA 5125 0.0 37.509445 1 ACCTGGG 1580 0.0 33.69396 3 TCGACGT 60 0.0039377604 31.688364 3 TATCAAC 6225 0.0 30.466646 2 ATCAACG 6225 0.0 30.39029 3 GTACATG 5165 0.0 30.199995 1 TCAACGC 6275 0.0 30.148132 4 CAACGCA 6400 0.0 29.485033 5 TTCGACG 65 0.005824593 29.2508 2 GACCGTC 65 0.0058403737 29.23461 7 TACATGG 5350 0.0 28.608374 2 AACGCAG 6655 0.0 28.489605 6 TAGGACC 1355 0.0 27.712704 4 CGACGTC 70 0.008363651 27.161457 4 ACATGGG 5260 0.0 26.296524 3 ACGCAGA 7405 0.0 25.661707 7 CGCAGAG 7445 0.0 25.460026 8 >>END_MODULE