FastQCFastQC Report
Thu 26 May 2016
SRR938540_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938540_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1174989
Sequences flagged as poor quality0
Sequence length101
%GC40

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47850.40723785499268506No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41440.35268415278781334No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27460.23370431552976242No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18990.16161853430117218No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA16380.13940556039248028No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13330.11344787057580964No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA12890.10970315466783093No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC12380.10536268850176471No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAACGC752.386514E-950.6651233
TACCTGG12350.049.237652
TAACGCA1155.4569682E-1141.301334
GTACCTG15900.039.150691
ACCTGGG15800.036.6760983
TATAACG1302.055458E-1036.5435682
GGTATCA34100.034.1410521
GTATCAA53550.034.075091
TAGGACC16100.028.6159214
GTACGAC1004.9542316E-528.499133
ATCAACG66250.027.5312373
TATCAAC67050.027.3490892
GTACATG50650.027.3010161
TCAACGC66900.027.2625834
ACTAACG700.00839463327.1408778
TACATGG50850.026.9064162
CAACGCA68400.026.595285
AACGCAG70500.025.9378226
AGTACCG1109.4815085E-525.90725
ACATGGG51300.025.5547773
Produced by FastQC (version 0.11.5)