##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938540_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1174989 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.836343148744373 31.0 31.0 34.0 30.0 34.0 2 32.09720005889417 33.0 31.0 34.0 30.0 34.0 3 32.152821005132814 33.0 31.0 34.0 30.0 34.0 4 35.79155124005416 37.0 35.0 37.0 35.0 37.0 5 35.700535068838946 37.0 35.0 37.0 33.0 37.0 6 35.74235077945411 37.0 35.0 37.0 33.0 37.0 7 35.676075265385464 37.0 35.0 37.0 33.0 37.0 8 35.65299334717176 37.0 35.0 37.0 33.0 37.0 9 37.19541799965787 39.0 37.0 39.0 33.0 39.0 10-11 37.1146661798536 39.0 37.0 39.0 33.0 39.0 12-13 37.017956338314654 39.0 37.0 39.0 33.0 39.0 14-15 38.03168199872509 40.0 37.0 41.0 33.0 41.0 16-17 38.13691872860086 40.0 37.0 41.0 33.0 41.0 18-19 38.29609341023618 40.0 38.0 41.0 33.5 41.0 20-21 38.279532829669044 40.0 38.0 41.0 33.0 41.0 22-23 38.135017434205764 40.0 37.0 41.0 33.0 41.0 24-25 38.03033049671103 40.0 37.0 41.0 33.0 41.0 26-27 37.829961812408456 40.0 37.0 41.0 33.0 41.0 28-29 37.697154611660196 40.0 37.0 41.0 32.0 41.0 30-31 37.418813708043224 39.0 36.0 41.0 32.0 41.0 32-33 37.264265452697856 39.0 36.0 41.0 31.5 41.0 34-35 37.113463189868156 39.0 36.0 41.0 31.0 41.0 36-37 36.973761882026125 39.0 36.0 41.0 31.0 41.0 38-39 36.7627548002577 39.0 35.0 41.0 30.0 41.0 40-41 36.52820877472044 39.0 35.0 40.5 30.0 41.0 42-43 36.315810616099384 39.0 35.0 40.0 30.0 41.0 44-45 36.03571012154156 39.0 35.0 40.0 29.5 41.0 46-47 35.568687877078 38.0 35.0 40.0 27.5 41.0 48-49 35.17253693438832 38.0 34.0 40.0 26.5 41.0 50-51 34.834388236826044 38.0 34.0 40.0 25.5 41.0 52-53 34.29734576238586 38.0 33.0 40.0 24.0 41.0 54-55 33.93815686785153 38.0 33.0 40.0 23.0 41.0 56-57 33.57038236102636 37.5 33.0 40.0 22.0 41.0 58-59 33.08551952401257 37.0 31.5 39.5 20.0 41.0 60-61 32.666677305064134 36.5 31.0 39.0 18.5 40.0 62-63 32.210050902604195 36.0 31.0 39.0 15.5 40.0 64-65 31.593154489105856 35.5 30.0 39.0 10.5 40.0 66-67 30.963900938647086 35.0 29.0 38.0 8.0 40.0 68-69 30.245590384250406 34.0 28.0 38.0 6.5 40.0 70-71 29.507942202012103 34.0 27.5 37.0 2.0 39.0 72-73 28.97563338890832 34.0 26.0 36.5 2.0 39.0 74-75 28.294109987412646 33.0 26.0 36.0 2.0 39.0 76-77 27.063696340987022 31.5 25.5 34.5 2.0 37.0 78-79 27.597966449047608 32.5 26.0 35.0 2.0 38.0 80-81 27.208960679631893 32.0 25.5 35.0 2.0 37.0 82-83 26.542404226762976 32.0 24.0 35.0 2.0 36.5 84-85 25.772921703947866 31.5 21.5 35.0 2.0 36.0 86-87 24.858963360508056 31.0 19.0 34.0 2.0 35.5 88-89 23.85227351064563 30.0 10.5 34.0 2.0 35.0 90-91 22.985233053245604 29.5 4.5 34.0 2.0 35.0 92-93 21.947074823679202 29.0 2.0 34.0 2.0 35.0 94-95 20.75217980764075 28.5 2.0 33.5 2.0 35.0 96-97 19.25186874089885 26.0 2.0 33.0 2.0 34.5 98-99 17.451382523581074 22.5 2.0 32.0 2.0 34.0 100-101 13.279009420513724 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3156238606453279 1101 2 0.2548714397521863 1101 3 0.3189445302437335 1101 4 0.09465610094740384 1101 5 0.22723846525177294 1101 6 0.22992302290358424 1101 7 0.3773920496013048 1101 8 0.3196607201705248 1101 9 0.40494934387552206 1101 10-11 0.46141828421496456 1101 12-13 0.4103254979237292 1101 14-15 0.48857688303309743 1101 16-17 0.5520718604102015 1101 18-19 0.4853180916210107 1101 20-21 0.4812484333938585 1101 22-23 0.5146324990979991 1101 24-25 0.5280271015022819 1101 26-27 0.6580861163412166 1101 28-29 0.6438206291958224 1101 30-31 0.6050274501676753 1101 32-33 0.6152552168657408 1101 34-35 0.5281978474365587 1101 36-37 0.7500258817153735 1101 38-39 0.9334422839512513 1101 40-41 0.8264510468207291 1101 42-43 0.812544491530744 1101 44-45 0.975045603950413 1101 46-47 1.1002891987622903 1101 48-49 1.3341665096869733 1101 50-51 1.357719401142944 1101 52-53 1.6958125092714056 1101 54-55 1.5676014360888786 1101 56-57 1.4607001158351736 1101 58-59 1.4736995593692228 1101 60-61 1.5704134533260188 1101 62-63 1.9214032833130474 1101 64-65 1.7579943554273072 1101 66-67 1.8329008749656843 1101 68-69 1.9472397319919956 1101 70-71 2.1007234310140888 1101 72-73 1.9495358315322555 1101 74-75 2.2564862803922736 1101 76-77 2.2203407496831495 1101 78-79 2.336450732782197 1101 80-81 2.53729306317328 1101 82-83 2.7232282304493047 1101 84-85 3.1906992709569693 1101 86-87 2.9393794814103806 1101 88-89 2.666608099387126 1101 90-91 3.0582188518057016 1101 92-93 3.190712738705674 1101 94-95 2.9123210221467666 1101 96-97 2.88811846633045 1101 98-99 2.7851769781786295 1101 100-101 2.070642159828658 1102 1 0.2712063133798672 1102 2 0.26634326124573704 1102 3 0.29268415070532683 1102 4 0.1770243451598219 1102 5 0.1084631427148608 1102 6 0.29197114702183313 1102 7 0.2763353310484078 1102 8 0.3701780760555664 1102 9 0.22246674298818192 1102 10-11 0.25097370790448537 1102 12-13 0.37331676987647455 1102 14-15 0.42256989587955474 1102 16-17 0.4057774489541046 1102 18-19 0.426251886572814 1102 20-21 0.29428195051003314 1102 22-23 0.3863011241063532 1102 24-25 0.4643434018477066 1102 26-27 0.4445425134379022 1102 28-29 0.40423828649520743 1102 30-31 0.35177575151913487 1102 32-33 0.4250443054312498 1102 34-35 0.5594242933018378 1102 36-37 0.36478575234693267 1102 38-39 0.6038514136222872 1102 40-41 0.6945490713189955 1102 42-43 0.7259639133025289 1102 44-45 0.6423124794195374 1102 46-47 0.6270549483486789 1102 48-49 0.6092901995956836 1102 50-51 0.8833722362846288 1102 52-53 0.7816157764926857 1102 54-55 0.8587987229669451 1102 56-57 1.2914781735007992 1102 58-59 1.1883607924564217 1102 60-61 1.105305993419627 1102 62-63 1.2771582535056396 1102 64-65 1.360539282667382 1102 66-67 1.437203201004035 1102 68-69 1.5082896913187476 1102 70-71 1.7536344175074277 1102 72-73 1.5859181522260997 1102 74-75 1.8109518888994636 1102 76-77 1.6582576504993654 1102 78-79 1.6638330652653863 1102 80-81 1.9938280952206568 1102 82-83 2.2846023968441607 1102 84-85 2.0718807208109773 1102 86-87 1.9545696632414256 1102 88-89 1.8310730398312707 1102 90-91 2.190371154461843 1102 92-93 2.320876178365829 1102 94-95 2.4569291638704485 1102 96-97 2.631749664088897 1102 98-99 2.214152051567318 1102 100-101 1.8182522625138766 1103 1 0.25756495919435096 1103 2 0.31070690384756006 1103 3 0.28641087447219604 1103 4 0.1401722587983656 1103 5 0.183302665368835 1103 6 0.18908863702514367 1103 7 0.1706963224209943 1103 8 0.2875260046657644 1103 9 0.3774809972441844 1103 10-11 0.3135146460029645 1103 12-13 0.33264574375719036 1103 14-15 0.454045445307834 1103 16-17 0.4204200320623457 1103 18-19 0.5098509926406933 1103 20-21 0.4295067279627247 1103 22-23 0.4603889452949801 1103 24-25 0.5237504310361842 1103 26-27 0.48172756300029107 1103 28-29 0.5346889018922951 1103 30-31 0.3794440577891294 1103 32-33 0.4591636762477762 1103 34-35 0.5881039655475249 1103 36-37 0.6265985381338481 1103 38-39 0.539101767639373 1103 40-41 0.47419449280206294 1103 42-43 0.45071953234896256 1103 44-45 0.5969826976936758 1103 46-47 0.44575375623124813 1103 48-49 0.5767849595190739 1103 50-51 0.6079184861988551 1103 52-53 0.8743109381552898 1103 54-55 0.964929768549375 1103 56-57 1.0177855414228034 1103 58-59 0.9562971380020429 1103 60-61 0.8169150244421317 1103 62-63 1.0469832099942877 1103 64-65 1.1619606534464708 1103 66-67 1.1404179724056966 1103 68-69 1.3354182385075113 1103 70-71 1.5580961244000022 1103 72-73 1.3414588314398408 1103 74-75 1.5049875588494963 1103 76-77 1.6024496649969109 1103 78-79 1.758468692795752 1103 80-81 1.8152974376534239 1103 82-83 1.8980718889934032 1103 84-85 2.160901590663695 1103 86-87 2.0408246771837675 1103 88-89 2.231931102375327 1103 90-91 2.5670047795343613 1103 92-93 3.171686830692984 1103 94-95 2.8333655062969783 1103 96-97 2.854121516144069 1103 98-99 2.849679381062085 1103 100-101 2.6534257308831535 1104 1 0.17908311081411554 1104 2 0.10079160745893745 1104 3 0.11017125888033519 1104 4 0.1035226256203643 1104 5 0.0229786540710748 1104 6 0.04656911167327138 1104 7 0.09936439439982081 1104 8 0.05820082095870305 1104 9 0.13338153193387825 1104 10-11 0.28945657029374416 1104 12-13 0.12941295833770283 1104 14-15 0.15028707412778175 1104 16-17 0.08574898761145278 1104 18-19 0.13608668539840352 1104 20-21 0.16820341442613795 1104 22-23 0.2708642447626559 1104 24-25 0.20880088016368603 1104 26-27 0.1955597722812712 1104 28-29 0.07476916455934912 1104 30-31 0.12548088506007815 1104 32-33 0.33948503778209016 1104 34-35 0.36524738732472883 1104 36-37 0.05934561747280043 1104 38-39 -0.007970391784610342 1104 40-41 0.08408121911554645 1104 42-43 0.1827884794898793 1104 44-45 0.12871176456246047 1104 46-47 0.4630445729505155 1104 48-49 0.42543128567387356 1104 50-51 0.3902696553514602 1104 52-53 0.4265775221217041 1104 54-55 0.4606775643606724 1104 56-57 0.6364046445888931 1104 58-59 0.7200270575321355 1104 60-61 0.5833402270972172 1104 62-63 0.7530918769412267 1104 64-65 0.9268082106310231 1104 66-67 0.8297283973744563 1104 68-69 0.7298632833018353 1104 70-71 1.0999971742392383 1104 72-73 1.1493247060394118 1104 74-75 1.0621247485015033 1104 76-77 1.5924453276746213 1104 78-79 1.6582846896693297 1104 80-81 1.813772713436066 1104 82-83 1.659249745852886 1104 84-85 1.773437710344183 1104 86-87 1.781504985444517 1104 88-89 1.6274282808953693 1104 90-91 1.96449890268471 1104 92-93 2.1070059569519906 1104 94-95 1.9270020771774057 1104 96-97 1.8885366141705155 1104 98-99 2.0026362358076817 1104 100-101 1.9417403481033588 1105 1 0.16041878316670832 1105 2 0.15898696268688894 1105 3 0.24838566929339834 1105 4 0.19273071870239988 1105 5 0.10633872780389453 1105 6 0.07382806287475319 1105 7 0.18605700514395096 1105 8 0.1040223213707634 1105 9 0.24888380145166167 1105 10-11 0.22651650224354825 1105 12-13 0.3318117488706065 1105 14-15 0.39756874171092704 1105 16-17 0.3026830111533698 1105 18-19 0.35298042164241394 1105 20-21 0.3294557980912671 1105 22-23 0.271825838952239 1105 24-25 0.33291226252689654 1105 26-27 0.3481701771474093 1105 28-29 0.35506697957421096 1105 30-31 0.42767201931008003 1105 32-33 0.49214915904543943 1105 34-35 0.4486721327726215 1105 36-37 0.5652127202789146 1105 38-39 0.42519616155930606 1105 40-41 0.4421685054338411 1105 42-43 0.21279968925382065 1105 44-45 0.24547318883859504 1105 46-47 0.4149638032793561 1105 48-49 0.389582658481217 1105 50-51 0.3870134521677784 1105 52-53 0.19115092204452822 1105 54-55 0.5039998387129359 1105 56-57 0.5985466000636563 1105 58-59 0.9784085824841071 1105 60-61 0.665559180786623 1105 62-63 0.6502380519803452 1105 64-65 0.8823366880962951 1105 66-67 1.0917463810588082 1105 68-69 1.0941791979153805 1105 70-71 1.2615252078374297 1105 72-73 1.614827470313486 1105 74-75 1.1408754605971652 1105 76-77 1.431048757890661 1105 78-79 1.6110916541415854 1105 80-81 1.639763038830214 1105 82-83 1.8498125523367364 1105 84-85 2.161801254438984 1105 86-87 2.0628128100859264 1105 88-89 1.977214616857509 1105 90-91 2.259686054853365 1105 92-93 2.072299156597463 1105 94-95 1.5585849863161236 1105 96-97 1.8315679011447124 1105 98-99 2.2246888665682327 1105 100-101 2.267162057161217 1106 1 0.050095679502852875 1106 2 0.2073233532417902 1106 3 0.0930204171928466 1106 4 0.09919554004386555 1106 5 0.15855950729234536 1106 6 0.09538594054466643 1106 7 0.1246446374804222 1106 8 0.15514593617458416 1106 9 0.04592757124630964 1106 10-11 0.24023483938982082 1106 12-13 0.20385534691123297 1106 14-15 0.2185890292958632 1106 16-17 0.08473986860440164 1106 18-19 0.19754955950593 1106 20-21 0.21582539900362718 1106 22-23 0.36247245848960574 1106 24-25 0.3037563304865287 1106 26-27 0.08046062006668109 1106 28-29 0.37649226314010065 1106 30-31 0.47984299013250364 1106 32-33 0.14054119790306885 1106 34-35 0.03675824336846745 1106 36-37 -0.008096704894725804 1106 38-39 0.04294900065327312 1106 40-41 0.27624347262882054 1106 42-43 0.2753044124893975 1106 44-45 0.30999602181811525 1106 46-47 -0.05646285431868847 1106 48-49 0.018934733613292565 1106 50-51 0.3595685336822356 1106 52-53 0.1400901692123 1106 54-55 -0.06664739913890827 1106 56-57 0.1687438423810974 1106 58-59 0.2670082376020275 1106 60-61 0.4405390855661935 1106 62-63 0.25890441635994677 1106 64-65 0.5658586073444241 1106 66-67 0.7283251783684541 1106 68-69 0.3561191939306845 1106 70-71 0.6148294116888451 1106 72-73 0.7224478527066402 1106 74-75 0.8429368523543452 1106 76-77 0.9396007340924228 1106 78-79 0.9734496994434281 1106 80-81 1.098606309851185 1106 82-83 1.00170940374203 1106 84-85 1.2100789051187846 1106 86-87 1.4667013211159698 1106 88-89 1.356307343418237 1106 90-91 1.2873535665498146 1106 92-93 1.3867117234956545 1106 94-95 1.43257627495219 1106 96-97 1.546563744697412 1106 98-99 1.7985523702128567 1106 100-101 1.9810081792989358 1107 1 0.03507996387471479 1107 2 0.041593143592066895 1107 3 0.08014332312590255 1107 4 0.020685446398168494 1107 5 0.03988545266028609 1107 6 0.0222710058374318 1107 7 -0.06741005938617661 1107 8 0.15554125712983335 1107 9 0.016479400415811085 1107 10-11 0.10864156632673172 1107 12-13 0.07057385894908208 1107 14-15 0.11569540951685298 1107 16-17 0.17046132934773794 1107 18-19 0.2557783920129779 1107 20-21 0.1671636576472153 1107 22-23 0.2580818036177561 1107 24-25 0.22496594780287893 1107 26-27 0.08579259245902193 1107 28-29 0.2094108335039877 1107 30-31 0.08060932952526656 1107 32-33 0.2927899908780276 1107 34-35 0.2541734275815557 1107 36-37 0.17796823143267204 1107 38-39 0.18392790606787202 1107 40-41 0.4148585531511628 1107 42-43 0.2074922871532081 1107 44-45 0.20570743752077902 1107 46-47 0.3114481012563104 1107 48-49 0.3462394223790497 1107 50-51 0.39623617244961196 1107 52-53 0.20922435151358343 1107 54-55 0.2704787471298502 1107 56-57 0.21942917338139267 1107 58-59 0.36139597617174957 1107 60-61 0.3499149969106057 1107 62-63 0.43671945799825806 1107 64-65 0.5674917365366099 1107 66-67 0.6084862230526831 1107 68-69 0.785147741288192 1107 70-71 0.6015229731418117 1107 72-73 0.80063715311816 1107 74-75 0.7078893814363596 1107 76-77 0.527812392820266 1107 78-79 0.9398587594220409 1107 80-81 0.9420608317396564 1107 82-83 1.1191472703050032 1107 84-85 1.1329605356557089 1107 86-87 1.3061507880807461 1107 88-89 1.843728360235577 1107 90-91 1.7210271351315214 1107 92-93 1.838362674534185 1107 94-95 1.6809220835779612 1107 96-97 1.8173407771979804 1107 98-99 1.8382173195015987 1107 100-101 1.790379230139802 1108 1 0.06021802495305906 1108 2 0.0673853921583003 1108 3 0.07897504183779347 1108 4 0.0031143207078585533 1108 5 -0.023627021306680263 1108 6 -0.029065825668638468 1108 7 0.0355838177691723 1108 8 -0.01053980958352696 1108 9 -0.04439756105216475 1108 10-11 0.1407918168067397 1108 12-13 0.0876408975311378 1108 14-15 0.12951840364417677 1108 16-17 0.14088999101522148 1108 18-19 0.07702572616769032 1108 20-21 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16.0 5 44.0 6 123.0 7 271.0 8 540.0 9 974.0 10 1653.0 11 2875.0 12 4377.0 13 6154.0 14 6952.0 15 9089.0 16 11792.0 17 15184.0 18 15033.0 19 12705.0 20 12738.0 21 13962.0 22 15661.0 23 17194.0 24 19530.0 25 22182.0 26 26245.0 27 30320.0 28 35895.0 29 42188.0 30 49358.0 31 57832.0 32 68443.0 33 81765.0 34 100390.0 35 124402.0 36 149036.0 37 154306.0 38 63959.0 39 1790.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.877747963714626 20.918320439129367 15.453294832329947 22.750636764826066 2 14.537818773923206 21.300962758745136 43.12420362377251 21.037014843559152 3 18.71421179129985 26.81017695801077 30.89760742420219 23.57800382648719 4 12.195092890231313 18.415066013383953 38.126910124264995 31.26293097211974 5 11.577319578854915 38.201273716219845 34.61451062862823 15.606896076297014 6 28.487926269948062 37.02936793450832 20.04716639900459 14.435539396539031 7 26.75020787428648 33.1165653465692 22.750425748666583 17.382801030477733 8 24.57812335104699 38.10549554548642 20.831872325504598 16.484508777961988 9 25.618347793080087 18.20140835720596 22.58265204862015 33.5975918010938 10-11 23.5374118396002 26.869528140263444 30.188240060119714 19.40481996001665 12-13 25.84632707199812 24.886828727758303 29.594234499216586 19.67260970102699 14-15 20.887940227525533 29.197549934510025 26.984805815203377 22.92970402276106 16-17 19.562651224819977 30.639223005491967 30.130239517135905 19.66788625255215 18-19 20.332573326218373 27.740727785536716 32.89728669800313 19.029412190241782 20-21 20.79287550777071 27.97796404902514 31.29501637887674 19.934144064327413 22-23 20.04252805770948 29.34401939081983 31.50463536254382 19.108817188926874 24-25 20.546781081178842 28.122565092225955 32.49796270960686 18.832691116988343 26-27 19.08526016050143 29.56585797449187 31.96929389840073 19.379587966605964 28-29 19.087410791528242 29.677575018947028 30.83005634532822 20.40495784419651 30-31 19.712950504217485 28.138433636400002 32.08123650519282 20.0673793541897 32-33 19.07395571185043 28.9912143708824 32.663254997714866 19.2715749195523 34-35 19.632937331230966 29.390761085824575 31.859933141396795 19.116368441547664 36-37 19.192197297395055 29.027577123817267 31.534476192097294 20.245749386690385 38-39 19.977494941894562 29.546400510023858 30.89712958368196 19.57897496439962 40-41 21.309751446240455 27.59132711101313 32.10013308008254 18.998788362663866 42-43 19.461161681284498 29.497078561792776 31.833122683382488 19.20863707354024 44-45 19.210439976391157 29.818078296418633 30.77051720300753 20.20096452418268 46-47 20.742577164552177 28.5576290501443 30.5672223314431 20.132571453860418 48-49 20.02980453434032 29.08405951034435 30.424965680529777 20.46117027478555 50-51 19.151583546739587 30.167090925957606 30.5109664856437 20.170359041659115 52-53 19.3304788385253 29.257422835447823 30.784415854105866 20.627682471921013 54-55 19.75486579023293 28.650098000917456 30.693223511028613 20.901812697821 56-57 20.248487432648304 29.32163620255168 28.997803383691252 21.43207298110876 58-59 20.05937927929538 29.877045657448708 28.82529113038505 21.23828393287086 60-61 19.55392773889798 28.175880795479785 31.78370180486796 20.48648966075427 62-63 20.07593262575224 28.185753228328096 30.974034650537153 20.76427949538251 64-65 20.690619231328974 29.125464153281435 29.241592900018638 20.942323715370954 66-67 20.268182831736997 30.356720358269317 28.722950649456756 20.652146160536926 68-69 20.625493893276925 28.236588762914632 30.828855580392954 20.309061763415492 70-71 21.242496738267334 28.25128575671772 29.954365530230497 20.551851974784444 72-73 20.37735704534855 29.732686631693262 29.004040892332515 20.885915430625673 74-75 19.942569675120364 29.961769854866727 29.94164200686134 20.154018463151573 76-77 20.12669905845927 28.774950233576657 30.7887988738618 20.309551834102276 78-79 19.80439816883392 28.570863216591814 30.447987172645874 21.176751441928392 80-81 19.93179499007223 29.410878588443957 30.00160852843478 20.65571789304903 82-83 19.69245316178933 29.158139893129686 30.611829778578365 20.537577166502622 84-85 19.625772494801804 29.08312601548862 30.47474438837477 20.816357101334805 86-87 18.675714481468464 30.50645861118369 28.98961907088259 21.828207836465257 88-89 19.398947564615497 29.155294219775673 29.83917296247029 21.60658525313854 90-91 19.66388192419577 28.99919487580151 30.638933287034437 20.697989912968286 92-93 19.122183417894806 29.885909036169444 30.074592536831368 20.917315009104385 94-95 19.123881159738517 29.97406784233725 29.546361710620268 21.355689287303967 96-97 20.219253116412155 29.463297103206926 29.1029533042437 21.214496476137224 98-99 20.031591785114582 28.68877921410328 30.24713422849065 21.032494772291486 100-101 18.99737742114072 29.27581844418052 30.56063952966348 21.166164605015283 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 867.0 1 594.0 2 943.5 3 1265.0 4 727.0 5 544.0 6 846.5 7 1505.0 8 2001.5 9 2013.0 10 1649.0 11 1309.0 12 1379.0 13 1519.0 14 1623.5 15 1767.5 16 1940.5 17 2118.0 18 2338.5 19 2626.0 20 2915.5 21 3325.0 22 3895.5 23 4624.0 24 5453.5 25 6520.5 26 7903.0 27 9535.0 28 11382.5 29 13704.0 30 16906.5 31 21401.5 32 27728.0 33 36412.0 34 47048.0 35 57518.0 36 65135.5 37 66953.0 38 61949.5 39 53696.5 40 46788.5 41 42615.5 42 39627.5 43 37475.0 44 36179.5 45 34912.5 46 33620.0 47 33040.5 48 32955.0 49 31642.0 50 29573.0 51 27425.0 52 25291.5 53 23710.0 54 22618.5 55 20725.0 56 17912.5 57 15686.5 58 13948.5 59 11911.0 60 9642.5 61 7637.0 62 6108.0 63 4875.5 64 3823.5 65 2968.5 66 2231.5 67 1782.0 68 1368.5 69 942.0 70 707.5 71 537.0 72 422.5 73 322.0 74 255.5 75 205.5 76 146.5 77 87.5 78 50.5 79 29.0 80 14.5 81 11.5 82 6.5 83 2.0 84 1.0 85 1.0 86 1.5 87 1.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02561726109776347 2 0.011574576442843295 3 0.0017872507742625675 4 0.0 5 1.7021435945357784E-4 6 0.0 7 0.0 8 8.510717972678892E-5 9 2.553215391803668E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.001829804364125962 26-27 0.009234129000356599 28-29 2.1276794931697235E-4 30-31 0.0 32-33 1.7021435945357784E-4 34-35 0.010340522336804854 36-37 9.787325668580728E-4 38-39 0.012085219521204029 40-41 0.011276701313799533 42-43 0.004595787705246602 44-45 4.680894884973392E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 5.957502580875225E-4 68-69 0.0011489469263116505 70-71 0.0 72-73 8.510717972678892E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.7021435945357784E-4 82-83 0.0011489469263116505 84-85 0.005276645143060914 86-87 0.003914930267432291 88-89 0.0 90-91 0.0012766076959018341 92-93 4.680894884973392E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 4.255358986339446E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1174989.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 72.4262162146562 #Duplication Level Percentage of deduplicated Percentage of total 1 87.83657999655696 63.61671134386581 2 8.003937572038607 11.593898263221572 3 1.7917041264512237 3.8929905136514407 4 0.735146651387014 2.129755612913454 5 0.4092784144400784 1.4821243468114393 6 0.2615472633695712 1.1365727188293717 7 0.17691954965334683 0.8969529489055029 8 0.1237373928606711 0.7169464935331856 9 0.0911706020649947 0.5942827563821718 >10 0.50877628744724 6.717259091519656 >50 0.03662844051645174 1.8316607821958462 >100 0.022339740365813505 3.032093394164539 >500 0.0011757699200282774 0.6373117432867813 >1k 0.0010581929280254496 1.721439990719172 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4785 0.40723785499268506 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4144 0.35268415278781334 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2746 0.23370431552976242 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1899 0.16161853430117218 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 1638 0.13940556039248028 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1333 0.11344787057580964 No Hit CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA 1289 0.10970315466783093 No Hit GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC 1238 0.10536268850176471 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.510717972678894E-5 0.0 10-11 0.0 0.0 0.0 1.276607695901834E-4 0.0 12-13 0.0 0.0 0.0 1.7021435945357787E-4 0.0 14-15 0.0 0.0 0.0 1.7021435945357787E-4 0.0 16-17 0.0 0.0 0.0 1.7021435945357787E-4 0.0 18-19 0.0 0.0 0.0 1.7021435945357787E-4 0.0 20-21 0.0 0.0 0.0 4.2553589863394465E-4 0.0 22-23 0.0 0.0 0.0 8.510717972678893E-4 0.0 24-25 0.0 0.0 0.0 0.0015744828249455952 0.0 26-27 0.0 0.0 0.0 0.0031489656498911904 0.0 28-29 0.0 0.0 0.0 0.0060426097606020145 0.0 30-31 0.0 0.0 0.0 0.01506397081164164 0.0 32-33 0.0 0.0 0.0 0.03297903214413071 0.0 34-35 0.0 0.0 0.0 0.06259633068905326 0.0 36-37 0.0 0.0 0.0 0.10791590389356837 0.0 38-39 0.0 0.0 0.0 0.17715059460131116 0.0 40-41 0.0 0.0 0.0 0.2578322009823071 0.0 42-43 0.0 0.0 0.0 0.33706698530794754 0.0 44-45 0.0 0.0 0.0 0.43855729713214336 0.0 46-47 8.510717972678894E-5 0.0 0.0 0.5548562582288005 0.0 48-49 8.510717972678894E-5 0.0 0.0 0.6834957603858418 0.0 50-51 8.510717972678894E-5 0.0 0.0 0.8241353748843606 0.0 52-53 8.510717972678894E-5 0.0 0.0 0.9589025939817308 0.0 54-55 8.510717972678894E-5 0.0 0.0 1.1197126100754984 0.0 56-57 8.510717972678894E-5 0.0 0.0 1.3086931026588333 0.0 58-59 8.510717972678894E-5 0.0 0.0 1.5101843506620063 0.0 60-61 8.510717972678894E-5 0.0 0.0 1.7377609492514399 0.0 62-63 8.510717972678894E-5 0.0 0.0 1.9652098870712833 0.0 64-65 8.510717972678894E-5 0.0 0.0 2.1975099341355535 0.0 66-67 8.510717972678894E-5 0.0 0.0 2.4705337666990923 0.0 68-69 8.510717972678894E-5 0.0 0.0 2.794749567868295 0.0 70-71 8.510717972678894E-5 0.0 0.0 3.129731427272936 0.0 72-73 8.510717972678894E-5 0.0 0.0 3.446117367907274 0.0 74-75 8.510717972678894E-5 0.0 0.0 3.7742480993439087 0.0 76-77 8.510717972678894E-5 4.255358986339447E-5 0.0 4.138038739086069 0.0 78-79 8.510717972678894E-5 8.510717972678894E-5 0.0 4.540553145603916 0.0 80-81 8.510717972678894E-5 8.510717972678894E-5 0.0 4.954088931896384 0.0 82-83 8.510717972678894E-5 8.510717972678894E-5 0.0 5.367411950239534 0.0 84-85 8.510717972678894E-5 8.510717972678894E-5 0.0 5.779203039347602 0.0 86-87 8.510717972678894E-5 8.510717972678894E-5 0.0 6.204228294903187 0.0 88-89 1.7021435945357787E-4 8.510717972678894E-5 0.0 6.657253812588884 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATAACGC 75 2.386514E-9 50.665123 3 TACCTGG 1235 0.0 49.23765 2 TAACGCA 115 5.4569682E-11 41.30133 4 GTACCTG 1590 0.0 39.15069 1 ACCTGGG 1580 0.0 36.676098 3 TATAACG 130 2.055458E-10 36.543568 2 GGTATCA 3410 0.0 34.141052 1 GTATCAA 5355 0.0 34.07509 1 TAGGACC 1610 0.0 28.615921 4 GTACGAC 100 4.9542316E-5 28.49913 3 ATCAACG 6625 0.0 27.531237 3 TATCAAC 6705 0.0 27.349089 2 GTACATG 5065 0.0 27.301016 1 TCAACGC 6690 0.0 27.262583 4 ACTAACG 70 0.008394633 27.140877 8 TACATGG 5085 0.0 26.906416 2 CAACGCA 6840 0.0 26.59528 5 AACGCAG 7050 0.0 25.937822 6 AGTACCG 110 9.4815085E-5 25.9072 5 ACATGGG 5130 0.0 25.554777 3 >>END_MODULE