FastQCFastQC Report
Thu 26 May 2016
SRR938539_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938539_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232609
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45300.14013448579769466No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34730.10743643911156592No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA64800.039.631311
GGTATCA44700.033.407331
ATCAACG78000.031.6445643
TATCAAC81750.030.6002062
TCAACGC81200.030.4560594
CAACGCA83400.029.7667085
AACGCAG87250.028.541016
GTACATG90550.025.7351841
TACATGG92250.024.6942312
ACGCAGA102050.024.07477
ACATGGG95850.023.2209593
CGCAGAG104900.023.1933988
GCAGAGT116700.020.114349
CATGGGG71600.019.5281314
GAGTACT56500.017.99636512-13
GTGTAGC17100.017.7993241
AGTACTT59650.017.48411212-13
AGAGTAC104000.017.47582610-11
GTGTAGG16400.016.8191411
GTACTTT60000.016.66709114-15
Produced by FastQC (version 0.11.5)