##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938539_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3232609 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.081226340704983 31.0 31.0 34.0 28.0 34.0 2 31.12845259046176 31.0 31.0 34.0 27.0 34.0 3 31.182892518086785 31.0 31.0 34.0 27.0 34.0 4 34.82639471708456 37.0 35.0 37.0 32.0 37.0 5 34.79357385938108 37.0 35.0 37.0 32.0 37.0 6 34.80367344148333 37.0 35.0 37.0 32.0 37.0 7 34.70130009537188 37.0 35.0 37.0 30.0 37.0 8 34.67002814135579 37.0 35.0 37.0 30.0 37.0 9 36.16063495461406 39.0 35.0 39.0 31.0 39.0 10-11 35.98737227422184 39.0 35.0 39.0 30.0 39.0 12-13 35.84128733168781 38.0 35.0 39.0 30.0 39.0 14-15 36.83250510655634 39.0 36.0 41.0 30.5 41.0 16-17 36.645702898185334 39.0 36.0 41.0 30.0 41.0 18-19 36.76177013675331 39.0 36.0 41.0 30.0 41.0 20-21 36.726533737918814 39.0 36.0 41.0 30.0 41.0 22-23 36.63316333648765 39.0 36.0 41.0 30.0 41.0 24-25 36.502495352824916 39.0 36.0 41.0 29.5 41.0 26-27 36.2981276114742 39.0 35.5 41.0 29.0 41.0 28-29 36.08549007937552 39.0 35.0 40.0 27.5 41.0 30-31 35.866096858605545 38.5 35.0 40.0 27.0 41.0 32-33 35.63362333644434 38.0 34.5 40.0 27.0 41.0 34-35 35.39262821454744 38.0 34.0 40.0 26.0 41.0 36-37 35.141548668583184 38.0 34.0 40.0 25.0 41.0 38-39 35.06335316148659 38.0 34.0 40.0 25.0 41.0 40-41 34.98349738554833 38.0 34.0 40.0 25.0 41.0 42-43 34.72025382593441 38.0 33.5 40.0 24.0 41.0 44-45 34.42553491622401 38.0 33.0 40.0 24.0 41.0 46-47 34.098562183054 38.0 33.0 40.0 23.0 41.0 48-49 33.78311991954486 38.0 33.0 40.0 22.0 41.0 50-51 33.10379356117613 37.5 31.5 39.5 20.5 40.5 52-53 33.136747129021785 37.0 32.0 39.5 20.5 40.0 54-55 33.24638132851824 37.0 32.0 40.0 19.5 41.0 56-57 32.93121670452566 37.0 31.5 40.0 18.0 41.0 58-59 32.55840359907431 36.5 31.0 39.5 15.0 41.0 60-61 32.077404195805926 36.0 31.0 39.0 11.5 40.5 62-63 31.50059765966128 35.5 30.0 39.0 8.0 40.0 64-65 30.97366662655459 35.0 29.0 38.5 7.0 40.0 66-67 30.42939263610291 35.0 29.0 38.0 2.0 40.0 68-69 29.84088796387067 34.0 28.0 37.5 2.0 40.0 70-71 29.202335945980476 34.0 27.5 37.0 2.0 39.0 72-73 28.54993907398018 34.0 26.0 36.0 2.0 39.0 74-75 27.85434102918107 33.0 26.0 36.0 2.0 38.5 76-77 27.101403386552473 32.5 24.5 35.0 2.0 37.5 78-79 26.316604018611592 32.0 22.5 35.0 2.0 37.0 80-81 25.60979088408156 31.5 20.5 35.0 2.0 36.0 82-83 24.880944153777953 31.0 18.0 34.0 2.0 36.0 84-85 24.172735397321482 31.0 12.5 34.0 2.0 35.0 86-87 23.40055339201246 30.0 6.5 34.0 2.0 35.0 88-89 22.590068579280697 29.5 2.0 34.0 2.0 35.0 90-91 21.672857589643534 29.0 2.0 34.0 2.0 35.0 92-93 20.72379430979744 28.0 2.0 34.0 2.0 35.0 94-95 19.715215016724883 26.5 2.0 33.0 2.0 35.0 96-97 18.453354705131368 25.0 2.0 33.0 2.0 34.5 98-99 16.93036708120283 17.0 2.0 32.0 2.0 34.0 100-101 13.417315858490774 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4375425521364704 1101 2 0.472289430055973 1101 3 0.4258992197243181 1101 4 0.39477227832671247 1101 5 0.34893536899067357 1101 6 0.4846348644453684 1101 7 0.3919896036856372 1101 8 0.516176104983451 1101 9 0.6362528045903275 1101 10-11 0.6065163365111346 1101 12-13 0.7366452091949256 1101 14-15 0.8633550161367296 1101 16-17 0.6993535814435106 1101 18-19 0.7481180535249408 1101 20-21 0.8048884459979604 1101 22-23 0.8140945338112715 1101 24-25 0.7102925570684064 1101 26-27 0.7196421339090051 1101 28-29 0.7894148441922653 1101 30-31 0.7070764243398031 1101 32-33 0.670136406935022 1101 34-35 0.7903059225953442 1101 36-37 0.8040204895569758 1101 38-39 0.832153569213169 1101 40-41 0.8390664449537439 1101 42-43 1.0605796868271895 1101 44-45 0.9661706318882892 1101 46-47 0.8286161124994678 1101 48-49 0.6595413832280315 1101 50-51 0.8333074472410615 1101 52-53 0.783113910813519 1101 54-55 0.7827936928199719 1101 56-57 0.5724178726902593 1101 58-59 0.7372809283213755 1101 60-61 0.7510107190574047 1101 62-63 0.7133722796178006 1101 64-65 0.7029327334575477 1101 66-67 0.6147399453973073 1101 68-69 0.5482769274756514 1101 70-71 0.8102880243072903 1101 72-73 0.7969691054997838 1101 74-75 0.8336563748914223 1101 76-77 0.8863745919837172 1101 78-79 1.1323941364268038 1101 80-81 1.1591132001872815 1101 82-83 1.1981323322712925 1101 84-85 1.1207287789041978 1101 86-87 1.1010651141866674 1101 88-89 1.203095047555113 1101 90-91 1.237768866931102 1101 92-93 1.1451952774521708 1101 94-95 1.0101221436600234 1101 96-97 0.7583301967086271 1101 98-99 0.8542960513215228 1101 100-101 0.9169229795935596 1102 1 0.45377725115636025 1102 2 0.4135434392777704 1102 3 0.3538333107083389 1102 4 0.2375319926927162 1102 5 0.29219830643346256 1102 6 0.27062395889109325 1102 7 0.31279864227307286 1102 8 0.4641117580529084 1102 9 0.6682605435854754 1102 10-11 0.682276253791251 1102 12-13 0.5165051297719003 1102 14-15 0.6948024649136713 1102 16-17 0.7449111360497795 1102 18-19 0.7002683936513776 1102 20-21 0.5278233533482322 1102 22-23 0.5520701554663319 1102 24-25 0.6683072426339507 1102 26-27 0.5403420031334107 1102 28-29 0.47511250962025287 1102 30-31 0.6655286311975885 1102 32-33 0.6420039055888012 1102 34-35 0.4809979441065977 1102 36-37 0.5347972718650666 1102 38-39 0.5029614303492664 1102 40-41 0.6416423454292826 1102 42-43 0.646217930211094 1102 44-45 0.786791857175551 1102 46-47 0.7716295283799894 1102 48-49 0.769430953992071 1102 50-51 0.8709885392565297 1102 52-53 0.7457247776688902 1102 54-55 0.6323442596450448 1102 56-57 0.6710593489630412 1102 58-59 0.655241270582934 1102 60-61 0.6211368554941856 1102 62-63 0.8991626202780196 1102 64-65 0.841061512095127 1102 66-67 0.5768424966988626 1102 68-69 0.7200812068047853 1102 70-71 1.0122173470058833 1102 72-73 1.0519647862157981 1102 74-75 1.0365111743400597 1102 76-77 1.013908957863375 1102 78-79 1.074234908897676 1102 80-81 1.032355755947453 1102 82-83 1.0422317900157019 1102 84-85 1.008905587744188 1102 86-87 1.1900570823986243 1102 88-89 1.137076332358614 1102 90-91 1.0040217673862273 1102 92-93 1.1883489179747073 1102 94-95 1.1030501845065963 1102 96-97 1.1770498479004416 1102 98-99 1.3571059408137884 1102 100-101 1.0686907657809552 1103 1 0.4077985051803594 1103 2 0.3143701878876648 1103 3 0.3299682609570951 1103 4 0.3063606435560473 1103 5 0.24444077058330294 1103 6 0.42464704200493486 1103 7 0.4157535369174994 1103 8 0.37032517625601 1103 9 0.48799459392202493 1103 10-11 0.6261872136976052 1103 12-13 0.671230126113727 1103 14-15 0.8229713630699464 1103 16-17 0.7519599910395343 1103 18-19 0.7279722457145965 1103 20-21 0.6453926758533655 1103 22-23 0.7414015316735245 1103 24-25 0.6976572755575674 1103 26-27 0.801673214722527 1103 28-29 0.9066176676536131 1103 30-31 0.9632678761493025 1103 32-33 0.9583609987992219 1103 34-35 0.8668581650606413 1103 36-37 1.08525042887414 1103 38-39 0.8650183333343406 1103 40-41 1.0511869935123954 1103 42-43 1.0509497856398369 1103 44-45 1.0611050339387944 1103 46-47 0.7923302526995641 1103 48-49 0.9502593487828221 1103 50-51 1.1543717152881285 1103 52-53 1.0458601303174149 1103 54-55 1.0380912599377012 1103 56-57 1.1768872864813318 1103 58-59 1.1397191568867058 1103 60-61 1.226304948850931 1103 62-63 1.2209772603131341 1103 64-65 1.2073138899119265 1103 66-67 1.2134640763008555 1103 68-69 1.475113490240549 1103 70-71 1.4433544007079604 1103 72-73 1.2328869218611018 1103 74-75 1.3551363389581432 1103 76-77 1.4315176058001633 1103 78-79 1.3617152461818485 1103 80-81 1.5276732859445268 1103 82-83 1.6182327594333223 1103 84-85 1.7366958643025718 1103 86-87 1.9147699152929754 1103 88-89 1.638795676812812 1103 90-91 1.7120830236051319 1103 92-93 1.4919229595316281 1103 94-95 1.5410714751123926 1103 96-97 1.7496098420473523 1103 98-99 1.8905554213522713 1103 100-101 1.637782348309491 1104 1 0.36662389139545226 1104 2 0.28867202983656526 1104 3 0.3446834273266717 1104 4 0.23535648431659695 1104 5 0.24581480504557618 1104 6 0.4152423331517028 1104 7 0.2887760413738292 1104 8 0.48130631113420463 1104 9 0.4694822647479313 1104 10-11 0.5570027217189946 1104 12-13 0.6314620408493141 1104 14-15 0.7733668621786833 1104 16-17 0.8186888606176765 1104 18-19 0.7962701141043809 1104 20-21 0.7577199931439154 1104 22-23 0.7821444386848171 1104 24-25 0.6898447755575674 1104 26-27 0.8013575581568659 1104 28-29 0.9617554424605075 1104 30-31 0.8929135980744363 1104 32-33 0.6517238553054554 1104 34-35 0.6864975738544672 1104 36-37 0.8622251392128248 1104 38-39 1.0279296152713542 1104 40-41 1.0036760383727596 1104 42-43 1.0673639270539752 1104 44-45 0.8637175562204291 1104 46-47 0.9650779502634421 1104 48-49 0.7348423107554893 1104 50-51 0.8019736537908102 1104 52-53 0.9059128635319169 1104 54-55 0.9770767025996108 1104 56-57 0.9794765987213836 1104 58-59 0.9053673669282958 1104 60-61 1.0521646487915106 1104 62-63 1.037905736248966 1104 64-65 1.1863227282957673 1104 66-67 1.1808168317969923 1104 68-69 1.2380971875073357 1104 70-71 1.1134050914387998 1104 72-73 1.0936405983316888 1104 74-75 1.1031634854227903 1104 76-77 1.066562911801352 1104 78-79 1.1383650159382306 1104 80-81 1.1169112538589623 1104 82-83 1.1100860348343922 1104 84-85 1.3699493550928281 1104 86-87 1.646554674651263 1104 88-89 1.3226934783576745 1104 90-91 1.483218087479166 1104 92-93 1.367275752163831 1104 94-95 1.4069360027416273 1104 96-97 1.466261654287404 1104 98-99 1.5992879674009934 1104 100-101 1.6004559594206 1105 1 0.33526926200160645 1105 2 0.3125460014229553 1105 3 0.2796210387348701 1105 4 0.2010592637448667 1105 5 0.10082931365485592 1105 6 0.21075739664061643 1105 7 0.22025705128621098 1105 8 0.1887699924540982 1105 9 0.4440898406626985 1105 10-11 0.4615673534078084 1105 12-13 0.43749623904037804 1105 14-15 0.5203331009749874 1105 16-17 0.45103001256666886 1105 18-19 0.45800966963478373 1105 20-21 0.4309367545959688 1105 22-23 0.48384947535717515 1105 24-25 0.537970445578658 1105 26-27 0.5130983712048689 1105 28-29 0.4759913276114318 1105 30-31 0.5568773878552236 1105 32-33 0.5261630810518696 1105 34-35 0.765366719717079 1105 36-37 0.6756354640610596 1105 38-39 0.5647583759563233 1105 40-41 0.6988647760451627 1105 42-43 0.8120721358144749 1105 44-45 0.6995124802506538 1105 46-47 0.6404780766439657 1105 48-49 0.41948754932230514 1105 50-51 0.5043050914163629 1105 52-53 0.49787040973782126 1105 54-55 0.6284165875996237 1105 56-57 0.7693909932109406 1105 58-59 0.7084236062590534 1105 60-61 0.7227068275036288 1105 62-63 0.97318130551146 1105 64-65 0.9579001583699274 1105 66-67 0.8509613205689313 1105 68-69 0.8392275386376866 1105 70-71 0.7470854996290122 1105 72-73 0.934512749661355 1105 74-75 1.0335999697808447 1105 76-77 1.0328064237964973 1105 78-79 1.065266303950633 1105 80-81 1.161221458299888 1105 82-83 1.2252337774331146 1105 84-85 1.2926442467419683 1105 86-87 1.3163557039294176 1105 88-89 1.4330775871077925 1105 90-91 1.3067286364799102 1105 92-93 1.3129203399652845 1105 94-95 1.4040586452891048 1105 96-97 1.6042584916306453 1105 98-99 1.7658563905053661 1105 100-101 1.7155699062338936 1106 1 0.2722442983432316 1106 2 0.1557085046870661 1106 3 0.06973716436168687 1106 4 0.12665895290354 1106 5 0.15315776094239197 1106 6 -0.05014526506040795 1106 7 0.23386211942413837 1106 8 0.13620851890423324 1106 9 0.057295918965103 1106 10-11 0.11992877207867281 1106 12-13 0.1979889665263812 1106 14-15 0.394897594964128 1106 16-17 0.2220806625266576 1106 18-19 0.06554858414782672 1106 20-21 0.20251551014146685 1106 22-23 0.22222923240941128 1106 24-25 0.1639115400305755 1106 26-27 0.11811894384553057 1106 28-29 0.36538981962604566 1106 30-31 -0.06254455689992255 1106 32-33 0.21391609346178342 1106 34-35 -0.0921905227042501 1106 36-37 0.07245082417477988 1106 38-39 0.11414615399942107 1106 40-41 0.3087702598949491 1106 42-43 -0.1397405641342786 1106 44-45 0.05239488420193794 1106 46-47 0.1500329388809334 1106 48-49 0.37855871187722556 1106 50-51 0.0809940343463964 1106 52-53 0.03712887653323804 1106 54-55 0.16326300321933473 1106 56-57 -0.5257970755651513 1106 58-59 -0.1738669738244809 1106 60-61 0.41360045790965927 1106 62-63 0.4256697816209467 1106 64-65 0.2873455314283895 1106 66-67 -0.10010604444340387 1106 68-69 -0.03345293365680391 1106 70-71 -0.053479628331206897 1106 72-73 0.25139407977177086 1106 74-75 0.6520524554641298 1106 76-77 0.6796065979290802 1106 78-79 0.8879661340072857 1106 80-81 0.8629481916312649 1106 82-83 0.5565219780304993 1106 84-85 0.6204031031363506 1106 86-87 0.47003486569118635 1106 88-89 0.5298725945157621 1106 90-91 0.6335312124412376 1106 92-93 0.6221349314938074 1106 94-95 0.6478259346159057 1106 96-97 0.6090015797400561 1106 98-99 0.9435767932026771 1106 100-101 0.454542091102093 1107 1 0.3485617846899096 1107 2 0.21432002037920483 1107 3 0.28068107535431963 1107 4 0.17512584261051956 1107 5 0.14832853991480022 1107 6 0.38726241664569017 1107 7 0.3819592896001893 1107 8 0.30829230803499996 1107 9 0.5429636218627749 1107 10-11 0.5720286248500983 1107 12-13 0.5275056615881084 1107 14-15 0.6610283605870819 1107 16-17 0.7963549145553444 1107 18-19 0.7249315048085165 1107 20-21 0.7624368211350614 1107 22-23 0.7901119769314207 1107 24-25 0.7725403863257227 1107 26-27 0.704402986162755 1107 28-29 0.6451139497310621 1107 30-31 0.6109064957775558 1107 32-33 0.7974453939406274 1107 34-35 0.6156244376147697 1107 36-37 0.5660022346918723 1107 38-39 0.8150919637188494 1107 40-41 0.7428105341905891 1107 42-43 0.7747797773069252 1107 44-45 0.6324754891026387 1107 46-47 0.6508746190584205 1107 48-49 0.7009345375486973 1107 50-51 0.6632601063991288 1107 52-53 0.8055954967102892 1107 54-55 0.8737979840844261 1107 56-57 0.8697857408544039 1107 58-59 0.8012770015119841 1107 60-61 0.7756208319691424 1107 62-63 0.9907830631633274 1107 64-65 0.947274808147391 1107 66-67 1.0404891377347916 1107 68-69 1.1158775934750764 1107 70-71 1.2284962355208116 1107 72-73 1.3796591688127862 1107 74-75 1.3500121052880942 1107 76-77 1.5216070782760553 1107 78-79 1.3872173729866013 1107 80-81 1.5013682930019918 1107 82-83 1.3357223273550254 1107 84-85 1.4392793315576462 1107 86-87 1.3600931768612625 1107 88-89 1.4523511076581777 1107 90-91 1.533981061332799 1107 92-93 1.8317902919806528 1107 94-95 1.8742687609651547 1107 96-97 2.087838758939352 1107 98-99 2.1076752430450405 1107 100-101 1.77113744392536 1108 1 0.333688042029582 1108 2 0.18390330058828042 1108 3 0.203597646922006 1108 4 0.30758835787226246 1108 5 0.270033721012112 1108 6 0.28359167475822034 1108 7 0.28892809031417954 1108 8 0.43009062054625247 1108 9 0.3848810481407341 1108 10-11 0.473503590339007 1108 12-13 0.3870940522195596 1108 14-15 0.5233773762011538 1108 16-17 0.5718020896303315 1108 18-19 0.5388906182814068 1108 20-21 0.5338424863583242 1108 22-23 0.48052085794305555 1108 24-25 0.629740152946276 1108 26-27 0.6265026247079319 1108 28-29 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2316 70-71 -1.903409084064858 2316 72-73 -1.8563629061979157 2316 74-75 -1.8157257698487363 2316 76-77 -2.1794275877374822 2316 78-79 -2.386867198972265 2316 80-81 -2.3446424634115175 2316 82-83 -2.4728550301284393 2316 84-85 -2.434824849657719 2316 86-87 -2.387528070478634 2316 88-89 -2.8011542084138448 2316 90-91 -2.837721954798205 2316 92-93 -2.904960089934807 2316 94-95 -3.0810526202809037 2316 96-97 -3.11703185484588 2316 98-99 -3.1470008153962254 2316 100-101 -2.6357011486415693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11779.0 3 4336.0 4 4569.0 5 5405.0 6 6233.0 7 7734.0 8 9450.0 9 11894.0 10 14783.0 11 17818.0 12 20735.0 13 23934.0 14 26604.0 15 29774.0 16 32864.0 17 37185.0 18 38308.0 19 38783.0 20 42578.0 21 47098.0 22 51420.0 23 57115.0 24 64144.0 25 71840.0 26 79423.0 27 90305.0 28 102418.0 29 116841.0 30 133863.0 31 155159.0 32 181105.0 33 216495.0 34 260899.0 35 315755.0 36 374355.0 37 363816.0 38 159234.0 39 6558.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63113306661684 18.030433152373657 13.887314549331208 24.451119231678298 2 15.794770823662319 19.164944593795706 39.80981378696754 25.23047079557444 3 19.5918578455451 22.70388679972225 28.647440277054258 29.056815077678387 4 13.25483005754567 15.54940936712154 35.72028097647999 35.47547959885281 5 14.770319685352623 36.37707491217623 33.667783531826316 15.18482187064483 6 34.27089542417885 34.4592851779314 16.98446341897677 14.28535597891298 7 30.591843977885645 30.97628001201827 19.76061244893242 18.671263561163666 8 26.82824488305564 35.234747413485 19.056059337833595 18.88094836562577 9 26.515193005613813 16.723182302143446 19.38945786142855 37.37216683081419 10-11 25.745874548131265 25.858243018909505 26.969810316883418 21.426072116075808 12-13 26.18768018016624 22.822284552961282 27.38206149943327 23.607973767439212 14-15 22.967793481461175 24.624031911471718 25.080356550232764 27.327818056834342 16-17 23.464096348557515 27.893599945660895 25.58780848312045 23.05449522266114 18-19 23.36309859983072 26.762311633211045 27.16746173092426 22.70712803603397 20-21 23.720901366982254 26.103919499624382 27.319076742977433 22.856102390415924 22-23 23.561954638823064 25.771558998803677 27.17618411337633 23.490302248996926 24-25 23.454178668821612 25.957647390898902 27.274105954874273 23.314067985405217 26-27 23.289625923089734 26.37606841310591 27.086744589296647 23.24756107450771 28-29 23.15934995761932 26.48181871221856 27.28424272916402 23.0745886009981 30-31 23.337354046636662 26.115732463501306 27.229448895020997 23.31746459484103 32-33 23.195528730884625 26.13246085233103 27.208417242105586 23.46359317467876 34-35 23.628665207979065 26.18296783855402 26.965919983086362 23.222446970380553 36-37 23.59386483528099 26.00298280592061 26.946109081803694 23.45704327699471 38-39 23.179775446055917 26.25102602724571 27.106521935901633 23.46267659079674 40-41 23.557342074332745 26.02958133443421 27.080190953535997 23.332885637697046 42-43 23.378000447161106 25.939552792474696 27.086141951216113 23.59630480914808 44-45 23.31276424207962 26.229272871383678 26.961400933931206 23.496561952605497 46-47 23.337908690151092 26.441287673407082 26.633171115199524 23.587632521242305 48-49 23.595847670362893 26.147586274319323 26.815886243443536 23.440679811874247 50-51 23.477056460270408 26.313837156276936 26.68504043218413 23.52406595126853 52-53 23.48791849207114 26.358559583970976 26.61500438777709 23.53851753618079 54-55 23.30614720604958 26.209225256475843 26.75287561244585 23.73175192502872 56-57 23.28088066037152 26.428102916970904 26.930590448374442 23.36042597428313 58-59 23.550107775875066 26.131422364627298 26.69218489151325 23.626284967984386 60-61 23.45023731584776 26.16197197262552 26.949017705163953 23.43877300636277 62-63 23.34594018723568 26.262960646598728 26.843209028794767 23.54789013737083 64-65 23.25790656890542 26.12235604004769 26.856555393355773 23.763181997691117 66-67 23.237557239344223 26.219757624771017 26.749123910476293 23.793561225408467 68-69 23.051540376277742 26.044149001914636 26.993034340089228 23.911276281718393 70-71 23.110507552740142 26.099034356461733 26.875513197819455 23.914944892978674 72-73 23.30306408805621 26.045011500270913 26.695203057992124 23.956721353680752 74-75 23.0652737636232 26.275706920987 26.693686138840583 23.965333176549223 76-77 23.25921905705501 26.139987765373657 26.622843972002073 23.97794920556926 78-79 23.325572409389462 26.169312712746095 26.70548778582572 23.799627092038723 80-81 23.130108766335216 26.319748523867776 26.70594357069626 23.844199139100752 82-83 23.339901711000767 26.26207440184271 26.522081922555223 23.875941964601296 84-85 23.355370875578917 25.96607793156776 26.537256625300067 24.141294567553256 86-87 23.265216904350712 25.821740989467727 26.79599599277511 24.117046113406445 88-89 23.3203094113735 25.961997752340615 26.69195322157549 24.025739614710393 90-91 23.430093265095312 25.98640417845452 26.596876185619138 23.98662637083103 92-93 23.389007078482187 25.63035624492322 26.701643909797703 24.27899276679689 94-95 23.3132176157296 25.70876606731144 26.69311932277239 24.284896994186575 96-97 23.356998161499877 25.734251082387736 26.55723559897022 24.351515157142163 98-99 23.415784929114693 25.56303743392287 26.41980645377892 24.60137118318352 100-101 24.00896737569902 25.093234733271963 26.497904820931883 24.399893070097136 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 140.5 2 131.5 3 167.0 4 149.5 5 161.5 6 296.0 7 614.0 8 989.5 9 1191.5 10 1120.0 11 1011.5 12 1098.0 13 1269.0 14 1417.5 15 1550.5 16 1629.5 17 1716.5 18 1904.5 19 2139.0 20 2425.0 21 2801.0 22 3326.5 23 4108.5 24 5172.0 25 6669.0 26 8642.5 27 11361.0 28 14302.5 29 18000.5 30 22691.0 31 27767.0 32 33375.0 33 39166.0 34 45172.0 35 51295.0 36 58256.0 37 66183.0 38 73340.5 39 78450.5 40 85056.5 41 95433.0 42 105955.0 43 114550.5 44 122154.0 45 128514.5 46 132418.0 47 136541.5 48 140505.5 49 139678.5 50 136830.0 51 132595.5 52 126927.0 53 123683.0 54 122443.0 55 116059.0 56 104824.0 57 95540.0 58 87509.0 59 77478.5 60 64972.0 61 53348.0 62 43270.0 63 34702.0 64 28456.0 65 23144.5 66 17487.0 67 13493.5 68 10321.5 69 7118.5 70 5201.5 71 3866.5 72 2988.0 73 2338.5 74 1791.5 75 1452.0 76 1047.5 77 607.5 78 364.5 79 243.5 80 149.5 81 79.0 82 44.5 83 29.5 84 23.5 85 20.5 86 17.0 87 13.0 88 5.5 89 3.0 90 5.0 91 4.5 92 3.0 93 2.5 94 1.0 95 1.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006589104961348557 2 0.10697241763541461 3 0.11702621628535959 4 0.11968660608196044 5 0.15847880148820967 6 0.09694955375054638 7 0.026665767496161768 8 0.02920241823245558 9 0.023510421458332882 10-11 0.0532232633145549 12-13 0.05614659861430813 14-15 0.02841358172299836 16-17 0.03249697071312986 18-19 0.037554804803179104 20-21 0.059271009887060265 22-23 0.03201748185444017 24-25 0.049248146002192045 26-27 0.05825016263952739 28-29 0.05486280586362285 30-31 0.06971149310046468 32-33 0.05164559029564046 34-35 0.0643288439771095 36-37 0.05439878438747155 38-39 0.03376529608127676 40-41 0.03857565205071198 42-43 0.03438399138281184 44-45 0.007764626034265201 46-47 0.013564894486156538 48-49 0.009373233818256398 50-51 0.008754538516721323 52-53 0.010347678918174143 54-55 0.011894417172011833 56-57 0.015127100122532605 58-59 0.020741759983963416 60-61 0.025985202664473187 62-63 0.011523199991090787 64-65 0.00966711408648556 66-67 0.01076529824671032 68-69 0.012234699587856125 70-71 0.03197107970682504 72-73 0.029264287762609087 74-75 0.030594482660909503 76-77 0.02468594253124953 78-79 0.005444518653508668 80-81 0.0054135838884319136 82-83 0.006264289928042643 84-85 0.005274377445586521 86-87 0.013827839989308945 88-89 0.03859111943325035 90-91 0.036348348965185705 92-93 0.03043980883552573 94-95 0.04805715754673701 96-97 0.04462339862321734 98-99 0.027052452059621195 100-101 0.07596031564596893 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3232609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.76469327069353 #Duplication Level Percentage of deduplicated Percentage of total 1 75.60046095121785 38.378342113116176 2 12.780044298112525 12.975500575591163 3 4.533912295185193 6.904880009439072 4 2.1781806349874797 4.422986872932155 5 1.2566844097126408 3.1897599298562382 6 0.7974877165895027 2.429053159188712 7 0.48876253209126175 1.7368316016674281 8 0.3660185158472203 1.4864654150702892 9 0.28235949966738844 1.2900504053413318 >10 1.5173424219546776 14.105675041422202 >50 0.12178518829628732 4.290120506915874 >100 0.07291410348697272 6.81595516232735 >500 0.0030662370090338265 1.016001637039523 >1k 9.811958421553375E-4 0.9583775700923624 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4530 0.14013448579769466 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3473 0.10743643911156592 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5467382538376897E-5 0.0 14-15 0.0 0.0 0.0 3.0934765076753794E-5 0.0 16-17 0.0 0.0 0.0 3.0934765076753794E-5 0.0 18-19 0.0 0.0 0.0 6.186953015350759E-5 0.0 20-21 0.0 0.0 0.0 7.733691269188448E-5 0.0 22-23 0.0 0.0 0.0 1.5467382538376896E-4 0.0 24-25 0.0 0.0 0.0 2.7841288569078413E-4 0.0 26-27 0.0 0.0 0.0 5.413583888431914E-4 0.0 28-29 0.0 0.0 0.0 0.0021809009379111424 0.0 30-31 0.0 0.0 0.0 0.009063886167488861 0.0 32-33 0.0 0.0 0.0 0.024948888034401935 0.0 34-35 0.0 0.0 0.0 0.04816542922450565 0.0 36-37 0.0 0.0 0.0 0.09217013254618793 0.0 38-39 0.0 0.0 0.0 0.1577982366565211 0.0 40-41 0.0 0.0 0.0 0.2292266092187456 1.5467382538376897E-5 42-43 0.0 0.0 0.0 0.30551173989802044 3.0934765076753794E-5 44-45 0.0 0.0 0.0 0.39502148264760756 3.0934765076753794E-5 46-47 0.0 0.0 0.0 0.5067733214873806 3.0934765076753794E-5 48-49 0.0 0.0 0.0 0.6248822545504267 3.0934765076753794E-5 50-51 0.0 0.0 0.0 0.7496730968700513 3.0934765076753794E-5 52-53 0.0 0.0 0.0 0.8748506237531356 3.0934765076753794E-5 54-55 0.0 0.0 0.0 1.0149696421682919 3.0934765076753794E-5 56-57 0.0 0.0 0.0 1.1744383561389578 3.0934765076753794E-5 58-59 0.0 0.0 0.0 1.3519111033842943 3.0934765076753794E-5 60-61 0.0 0.0 0.0 1.543428233974477 3.0934765076753794E-5 62-63 0.0 0.0 0.0 1.741116850197472 3.0934765076753794E-5 64-65 0.0 0.0 0.0 1.9498955797004833 3.0934765076753794E-5 66-67 0.0 3.0934765076753794E-5 0.0 2.1854638157599635 3.0934765076753794E-5 68-69 0.0 3.0934765076753794E-5 0.0 2.4471719283093005 6.186953015350759E-5 70-71 0.0 3.0934765076753794E-5 0.0 2.7275337041999204 6.186953015350759E-5 72-73 0.0 3.0934765076753794E-5 0.0 3.010447598209372 7.733691269188448E-5 74-75 0.0 3.0934765076753794E-5 0.0 3.304126790465534 9.280429523026138E-5 76-77 0.0 3.0934765076753794E-5 0.0 3.6265289120954622 9.280429523026138E-5 78-79 0.0 3.0934765076753794E-5 0.0 3.975596801221552 9.280429523026138E-5 80-81 0.0 3.0934765076753794E-5 0.0 4.354392999586402 9.280429523026138E-5 82-83 0.0 3.0934765076753794E-5 0.0 4.742825377272661 9.280429523026138E-5 84-85 0.0 3.0934765076753794E-5 0.0 5.133113840863525 9.280429523026138E-5 86-87 0.0 3.0934765076753794E-5 0.0 5.53693626417547 9.280429523026138E-5 88-89 0.0 3.0934765076753794E-5 0.0 5.979504480746048 9.280429523026138E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6480 0.0 39.63131 1 GGTATCA 4470 0.0 33.40733 1 ATCAACG 7800 0.0 31.644564 3 TATCAAC 8175 0.0 30.600206 2 TCAACGC 8120 0.0 30.456059 4 CAACGCA 8340 0.0 29.766708 5 AACGCAG 8725 0.0 28.54101 6 GTACATG 9055 0.0 25.735184 1 TACATGG 9225 0.0 24.694231 2 ACGCAGA 10205 0.0 24.0747 7 ACATGGG 9585 0.0 23.220959 3 CGCAGAG 10490 0.0 23.193398 8 GCAGAGT 11670 0.0 20.11434 9 CATGGGG 7160 0.0 19.528131 4 GAGTACT 5650 0.0 17.996365 12-13 GTGTAGC 1710 0.0 17.799324 1 AGTACTT 5965 0.0 17.484112 12-13 AGAGTAC 10400 0.0 17.475826 10-11 GTGTAGG 1640 0.0 16.819141 1 GTACTTT 6000 0.0 16.667091 14-15 >>END_MODULE