FastQCFastQC Report
Thu 26 May 2016
SRR938539_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938539_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3232609
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45860.1418668326419929No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36640.1133449792412259No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46850.037.329891
GTATCAA66650.036.8645481
ATCAACG81150.029.4439683
TCAACGC83250.028.8153574
TATCAAC85100.028.3691272
CAACGCA85350.028.050715
AACGCAG88850.027.0421986
GTACATG92750.024.7486651
TACATGG95300.023.6871322
ACGCAGA105400.022.5707537
ACATGGG95750.022.473793
CGCAGAG107150.022.1578068
ATTCGCG6550.020.30957480-81
CGCGGAA6600.020.16382882-83
CATGGGG67750.019.0711334
GCAGAGT121550.019.0249239
TATACCG3003.7198333E-919.0010175
GAGTACT56200.018.54586812-13
TCGCGGA7300.017.90476882-83
AGTACTT59900.017.83629212-13
Produced by FastQC (version 0.11.5)