##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938539_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3232609 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.80296225123422 31.0 31.0 34.0 30.0 34.0 2 32.031643789892314 31.0 31.0 34.0 30.0 34.0 3 32.10240582761478 33.0 31.0 34.0 30.0 34.0 4 35.76402311569385 37.0 35.0 37.0 35.0 37.0 5 35.6562303080886 37.0 35.0 37.0 33.0 37.0 6 35.65048757829976 37.0 35.0 37.0 33.0 37.0 7 35.588511941902034 37.0 35.0 37.0 33.0 37.0 8 35.56892961691315 37.0 35.0 37.0 33.0 37.0 9 37.15783721446052 39.0 37.0 39.0 33.0 39.0 10-11 37.055064964553395 39.0 37.0 39.0 33.0 39.0 12-13 36.937555701911364 39.0 37.0 39.0 33.0 39.0 14-15 38.00953455861813 40.0 37.0 41.0 33.0 41.0 16-17 37.91617080816145 40.0 37.0 41.0 33.0 41.0 18-19 38.12796320247825 40.0 37.0 41.0 33.0 41.0 20-21 38.08896915772987 40.0 37.0 41.0 33.0 41.0 22-23 37.97946534826822 40.0 37.0 41.0 33.0 41.0 24-25 37.85693707466631 40.0 37.0 41.0 32.0 41.0 26-27 37.67794218230537 40.0 37.0 41.0 32.0 41.0 28-29 37.56671917327459 39.5 37.0 41.0 32.0 41.0 30-31 37.35246406231005 39.0 36.0 41.0 31.0 41.0 32-33 37.197348797828624 39.0 36.0 41.0 31.0 41.0 34-35 37.13872726333435 39.0 36.0 41.0 31.0 41.0 36-37 37.13096805088398 39.0 36.0 41.0 31.0 41.0 38-39 36.9940156696959 39.0 36.0 41.0 31.0 41.0 40-41 36.85181443224343 39.0 35.5 41.0 30.0 41.0 42-43 36.67390890763467 39.0 35.0 40.5 30.0 41.0 44-45 36.42788905184636 39.0 35.0 40.0 30.0 41.0 46-47 36.105673621523664 38.5 35.0 40.0 29.5 41.0 48-49 35.817590218922234 38.0 35.0 40.0 28.5 41.0 50-51 35.47405238307509 38.0 34.0 40.0 27.5 41.0 52-53 34.98411561682839 38.0 34.0 40.0 26.5 41.0 54-55 34.64835106874973 38.0 33.0 40.0 26.0 41.0 56-57 34.31475613041973 37.0 33.0 40.0 25.0 41.0 58-59 33.83236141457256 37.0 32.5 39.5 24.0 41.0 60-61 33.430000349562846 36.0 32.0 39.0 23.0 40.0 62-63 32.935877336232124 36.0 31.0 39.0 22.0 40.0 64-65 32.389652908842365 35.0 31.0 38.5 21.0 40.0 66-67 31.866931787914965 35.0 30.0 38.0 19.5 40.0 68-69 31.35884837912658 34.5 30.0 37.5 18.0 40.0 70-71 30.77482507163718 34.0 29.0 37.0 15.5 39.0 72-73 30.248804448666696 34.0 29.0 36.0 12.0 39.0 74-75 29.62381191167877 34.0 28.0 36.0 8.0 38.5 76-77 28.3952005949374 31.5 26.5 34.5 7.5 36.5 78-79 29.086254941442036 33.0 28.0 35.0 7.0 37.0 80-81 28.68889943076939 33.0 27.5 35.0 2.0 36.5 82-83 28.231680354784633 33.0 26.5 35.0 2.0 36.0 84-85 27.659529500784043 33.0 26.0 35.0 2.0 36.0 86-87 27.01734496810471 32.0 25.5 34.5 2.0 35.0 88-89 26.25944229568129 32.0 24.5 34.0 2.0 35.0 90-91 25.630519496790363 31.0 22.5 34.0 2.0 35.0 92-93 24.76349583262312 31.0 19.0 34.0 2.0 35.0 94-95 23.915853417471773 31.0 12.0 34.0 2.0 35.0 96-97 22.80726914390203 30.0 2.0 34.0 2.0 35.0 98-99 21.408255684495096 29.5 2.0 34.0 2.0 35.0 100-101 17.4460336836283 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2547164590349169 1101 2 0.2945235102798449 1101 3 0.3330668793184941 1101 4 0.20178086355254976 1101 5 0.09945155738579814 1101 6 0.23048344320246628 1101 7 0.21743836019808782 1101 8 0.3083140588825444 1101 9 0.2659340270368986 1101 10-11 0.24921460738760715 1101 12-13 0.36616073533900106 1101 14-15 0.35707943441286005 1101 16-17 0.46155335676498765 1101 18-19 0.445549329358343 1101 20-21 0.3371379035535256 1101 22-23 0.36286175945603105 1101 24-25 0.46845795133921087 1101 26-27 0.40213825573267314 1101 28-29 0.350187674908895 1101 30-31 0.38626292304512333 1101 32-33 0.39724374313059485 1101 34-35 0.33017274087059434 1101 36-37 0.39735717386702163 1101 38-39 0.47174639307016974 1101 40-41 0.5967845167993886 1101 42-43 0.5429157509035889 1101 44-45 0.5628875774094411 1101 46-47 0.5018860870105826 1101 48-49 0.6502266145070408 1101 50-51 0.6041742335503102 1101 52-53 0.6069261552036807 1101 54-55 0.659351227882226 1101 56-57 0.5761878323781744 1101 58-59 0.733359689659359 1101 60-61 0.6774725822803944 1101 62-63 0.7352980182048512 1101 64-65 0.6445564231156098 1101 66-67 0.4855782161361901 1101 68-69 0.6785384472191858 1101 70-71 0.6344879089812139 1101 72-73 0.630081354869322 1101 74-75 0.6541649161629302 1101 76-77 0.6008378730867889 1101 78-79 0.8411098791732314 1101 80-81 0.9909780811445899 1101 82-83 0.9500625841018149 1101 84-85 1.0474522674222548 1101 86-87 1.0160946663146966 1101 88-89 1.09698731331612 1101 90-91 1.1529361285588315 1101 92-93 1.4455574051503959 1101 94-95 1.5511932553031649 1101 96-97 1.4983207607638 1101 98-99 1.517020507806837 1101 100-101 1.5325223375903718 1102 1 0.26862991086694876 1102 2 0.29770390445914785 1102 3 0.2863121510664186 1102 4 0.17070546977357282 1102 5 0.10154467337731177 1102 6 0.12223504335883462 1102 7 0.12245130038826346 1102 8 0.17109702006275995 1102 9 0.24153772596102385 1102 10-11 0.30782759522531933 1102 12-13 0.3351144344201131 1102 14-15 0.46093392929408594 1102 16-17 0.43122441807252443 1102 18-19 0.3709214334167825 1102 20-21 0.2475086398059929 1102 22-23 0.3020885409006553 1102 24-25 0.3379905051212617 1102 26-27 0.4090228136938876 1102 28-29 0.23876820237568808 1102 30-31 0.24891817981585973 1102 32-33 0.3517165569567027 1102 34-35 0.3185208786920626 1102 36-37 0.3664546691925423 1102 38-39 0.4004312564622623 1102 40-41 0.3375401991795428 1102 42-43 0.2938705167556179 1102 44-45 0.30451315102448007 1102 46-47 0.252556988969701 1102 48-49 0.3417765074374657 1102 50-51 0.47762868149048643 1102 52-53 0.43260075632241524 1102 54-55 0.3401347510992139 1102 56-57 0.37879925263568026 1102 58-59 0.46805777739989907 1102 60-61 0.5458510865718083 1102 62-63 0.6031402424732235 1102 64-65 0.5610442153001642 1102 66-67 0.4593288028994422 1102 68-69 0.5247074719283944 1102 70-71 0.6557986007103231 1102 72-73 0.6634122057427589 1102 74-75 0.543719882731164 1102 76-77 0.5520991883941271 1102 78-79 0.8507469258740912 1102 80-81 0.7727088737734285 1102 82-83 0.7387896453873743 1102 84-85 0.7977993212035202 1102 86-87 0.7944825764798722 1102 88-89 0.8733742921172869 1102 90-91 0.7888358446360648 1102 92-93 1.0131032256691306 1102 94-95 1.0162277585521444 1102 96-97 1.0254630745969742 1102 98-99 1.2437612812981662 1102 100-101 1.4727711467945888 1103 1 0.27813660533578144 1103 2 0.20694446635555153 1103 3 0.208630705353805 1103 4 0.11053298660119992 1103 5 0.07056446412943274 1103 6 0.12535821426899219 1103 7 0.14459700568531275 1103 8 0.13844319360680402 1103 9 0.2333571216297372 1103 10-11 0.245293299790724 1103 12-13 0.3177586581543821 1103 14-15 0.45760955850099094 1103 16-17 0.4468631362463569 1103 18-19 0.42193703555315665 1103 20-21 0.3532483237392725 1103 22-23 0.30351065825505685 1103 24-25 0.28969338776685305 1103 26-27 0.4881400949347423 1103 28-29 0.4180304389867544 1103 30-31 0.3767258327277716 1103 32-33 0.3222087650339631 1103 34-35 0.47434790166543195 1103 36-37 0.4427665838837882 1103 38-39 0.338733769631709 1103 40-41 0.5023023260452106 1103 42-43 0.6311295341685508 1103 44-45 0.6810427274072737 1103 46-47 0.6532991421742693 1103 48-49 0.7542682765331605 1103 50-51 0.7991758171357901 1103 52-53 0.5608633953332429 1103 54-55 0.5130614777600186 1103 56-57 0.6106569677366522 1103 58-59 0.8223639121672335 1103 60-61 0.7065160521849236 1103 62-63 0.6393425032282707 1103 64-65 0.6445032985603305 1103 66-67 0.5251966145763447 1103 68-69 0.6295257518523059 1103 70-71 0.5167420452523181 1103 72-73 0.6835481985727263 1103 74-75 0.8560209692105296 1103 76-77 0.7198724544169544 1103 78-79 0.7490632486718667 1103 80-81 0.8999586542826492 1103 82-83 0.9423764905732099 1103 84-85 0.9639789926989835 1103 86-87 0.9646473774748934 1103 88-89 0.7616283344058452 1103 90-91 0.9347537733747266 1103 92-93 1.246650562514315 1103 94-95 0.995940865809974 1103 96-97 1.1091690849862026 1103 98-99 1.5318818000280494 1103 100-101 1.8931965930925116 1104 1 0.24299660236489729 1104 2 0.23839871472156204 1104 3 0.25542214920407247 1104 4 0.17285659028509315 1104 5 0.12297273804506403 1104 6 0.10588963138128804 1104 7 0.16542105500200677 1104 8 0.15113444434952328 1104 9 0.2280373800967581 1104 10-11 0.24737756152572388 1104 12-13 0.2737570613035274 1104 14-15 0.3639802507172689 1104 16-17 0.40198419982329625 1104 18-19 0.30871381065713877 1104 20-21 0.26263632136255666 1104 22-23 0.3020344996097819 1104 24-25 0.34762565158147396 1104 26-27 0.2924887283274913 1104 28-29 0.3825701374418955 1104 30-31 0.3084697424128535 1104 32-33 0.4173235154795947 1104 34-35 0.4244695222833741 1104 36-37 0.4822654326657272 1104 38-39 0.36970060712427966 1104 40-41 0.43374450518959407 1104 42-43 0.6225000407045016 1104 44-45 0.5128209879539156 1104 46-47 0.4706036207838906 1104 48-49 0.7093429200269199 1104 50-51 0.7118549986568752 1104 52-53 0.6417411062661031 1104 54-55 0.6571215639156947 1104 56-57 0.5279967256094906 1104 58-59 0.6213695939854134 1104 60-61 0.8030837512342472 1104 62-63 0.7355899037036124 1104 64-65 0.718529405215115 1104 66-67 0.6880986124967308 1104 68-69 0.7726527943359649 1104 70-71 0.8565927582647994 1104 72-73 0.7038941210326222 1104 74-75 0.8111466747957969 1104 76-77 0.8367767919095286 1104 78-79 0.9812286527122716 1104 80-81 0.7429287968851419 1104 82-83 1.0245029017437375 1104 84-85 1.2167224196152056 1104 86-87 1.2466493828224863 1104 88-89 1.3061219841384641 1104 90-91 1.2396640897740099 1104 92-93 1.4511217449266773 1104 94-95 1.2740667942116382 1104 96-97 1.6259870811240482 1104 98-99 1.9215923243893087 1104 100-101 2.1196894629677345 1105 1 0.16824504068523183 1105 2 0.1507167283467652 1105 3 0.07999530057460902 1105 4 0.17568443298222292 1105 5 -0.006322077524259839 1105 6 0.13292783162210497 1105 7 0.05230105952907138 1105 8 0.12086756767741491 1105 9 0.23052412114559218 1105 10-11 0.09727240161664241 1105 12-13 0.1705007194850907 1105 14-15 0.28365317039018834 1105 16-17 0.1924636912831872 1105 18-19 0.18705762854344243 1105 20-21 0.24766164462317874 1105 22-23 0.13841259742579126 1105 24-25 0.2244030741105263 1105 26-27 0.03142886105847964 1105 28-29 0.12479003325656635 1105 30-31 0.179311681882254 1105 32-33 0.22060925177186874 1105 34-35 0.09841878773590196 1105 36-37 0.1932493773097974 1105 38-39 0.03381608496524535 1105 40-41 0.15651767730916788 1105 42-43 0.19055689307507606 1105 44-45 0.09898216398438109 1105 46-47 0.08213128459913577 1105 48-49 0.26267109753811013 1105 50-51 0.29767664493604684 1105 52-53 0.24743860412046104 1105 54-55 0.3139890247047248 1105 56-57 0.2047211752097624 1105 58-59 0.29525912735207527 1105 60-61 0.2998275666055079 1105 62-63 0.21214191620334333 1105 64-65 0.23455332319981181 1105 66-67 0.1765270927552791 1105 68-69 0.2896410711281625 1105 70-71 0.33759245331678045 1105 72-73 0.32061533252507246 1105 74-75 0.5197036104769133 1105 76-77 0.3779542056359624 1105 78-79 0.5252119025778761 1105 80-81 0.4226626432856584 1105 82-83 0.4915355060312443 1105 84-85 0.5979986113489133 1105 86-87 0.6094243469046425 1105 88-89 0.7893647762047848 1105 90-91 0.8899366567132425 1105 92-93 1.183949556185862 1105 94-95 1.2533232554942408 1105 96-97 1.2131912108118392 1105 98-99 1.55575373306052 1105 100-101 1.443774540320785 1106 1 -0.06135983754065677 1106 2 0.04733091784826371 1106 3 0.09042475313918885 1106 4 0.10698532347419132 1106 5 -0.03039657052195821 1106 6 0.05378129074423299 1106 7 0.05749623604253884 1106 8 0.1198272406935672 1106 9 -0.18697218883554 1106 10-11 0.06756993164299985 1106 12-13 0.06490779245181244 1106 14-15 0.0405724794175768 1106 16-17 0.12136449854899212 1106 18-19 0.15896495984638648 1106 20-21 0.20007951464793905 1106 22-23 0.25733864134853235 1106 24-25 0.18969804521177736 1106 26-27 0.12935819143699945 1106 28-29 0.222122659307189 1106 30-31 0.20416458732699994 1106 32-33 0.1418761361391745 1106 34-35 0.12232475125324527 1106 36-37 0.21127155861715607 1106 38-39 0.08683113060698133 1106 40-41 0.1678493866152877 1106 42-43 0.14073587819071776 1106 44-45 -0.030633414248917745 1106 46-47 -0.7038748512354474 1106 48-49 -0.44298252105428304 1106 50-51 0.0825904374375881 1106 52-53 -0.008123916040233325 1106 54-55 0.22598312207095006 1106 56-57 0.0980138240777606 1106 58-59 0.0698812757040983 1106 60-61 0.08560259624434963 1106 62-63 -0.24995658041944324 1106 64-65 -0.0965518601421067 1106 66-67 -0.09043573545392292 1106 68-69 -0.2437549960169143 1106 70-71 -0.32622492181954144 1106 72-73 0.04894039909901693 1106 74-75 0.12622008953561803 1106 76-77 0.05680657485848073 1106 78-79 0.3906313812693263 1106 80-81 -0.03182287661949701 1106 82-83 0.538455213036066 1106 84-85 0.5872044334097097 1106 86-87 0.7928218628272319 1106 88-89 0.7673500782696614 1106 90-91 0.6589984667984119 1106 92-93 0.8041845618622752 1106 94-95 0.8400296221557433 1106 96-97 1.024800561745554 1106 98-99 1.1199035066323049 1106 100-101 1.0215441316328686 1107 1 0.13939129259663474 1107 2 0.19763265403305041 1107 3 0.1461211395153228 1107 4 0.11901708817764955 1107 5 0.120667403180164 1107 6 0.15249516944134456 1107 7 0.08698169481682072 1107 8 0.09791565887218212 1107 9 0.1690669812457415 1107 10-11 0.20964048977253213 1107 12-13 0.30604914184569765 1107 14-15 0.3923734629786537 1107 16-17 0.27733468956846963 1107 18-19 0.2691887967303046 1107 20-21 0.3435421862973058 1107 22-23 0.34441444930313736 1107 24-25 0.2932433767130007 1107 26-27 0.32719539398411257 1107 28-29 0.31904008666629835 1107 30-31 0.2849256694707947 1107 32-33 0.3479060615154381 1107 34-35 0.4894458505370878 1107 36-37 0.4006977534244598 1107 38-39 0.38307023524571093 1107 40-41 0.4339277054901345 1107 42-43 0.4271982137002084 1107 44-45 0.43209510249868543 1107 46-47 0.4725774687477369 1107 48-49 0.5545317090961248 1107 50-51 0.4887033763164368 1107 52-53 0.3581588936496587 1107 54-55 0.32907772055375517 1107 56-57 0.5078370914123056 1107 58-59 0.36076291498171287 1107 60-61 0.6180133131665073 1107 62-63 0.7409654540131001 1107 64-65 0.5830835740139619 1107 66-67 0.5532847063828967 1107 68-69 0.6608699487895358 1107 70-71 0.7315009648652655 1107 72-73 0.6284063212450413 1107 74-75 0.7256530723770354 1107 76-77 0.7918373201550999 1107 78-79 1.0734171825697985 1107 80-81 0.9167769926327303 1107 82-83 1.1612040801519754 1107 84-85 1.3319809175582584 1107 86-87 1.3259105677032856 1107 88-89 1.2441399887857223 1107 90-91 1.4308451983445387 1107 92-93 1.5698334783519456 1107 94-95 1.7146046404048043 1107 96-97 1.6894380285349975 1107 98-99 1.6269004693561442 1107 100-101 1.761930968889665 1108 1 0.18104097261808505 1108 2 0.14417312369870672 1108 3 0.21741153165891802 1108 4 0.08744606353796058 1108 5 0.13883327573756077 1108 6 0.1908946942874934 1108 7 0.18348630232955543 1108 8 0.16120094688960052 1108 9 0.20401041243789564 1108 10-11 0.14930222073660104 1108 12-13 0.20664578628149854 1108 14-15 0.2685843681163078 1108 16-17 0.3718313313283872 1108 18-19 0.29196362490940686 1108 20-21 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-1.0233985183401337 2316 74-75 -1.016602110964346 2316 76-77 -1.0266492999505665 2316 78-79 -1.143110862929877 2316 80-81 -1.2761696947334542 2316 82-83 -1.2654021414336576 2316 84-85 -1.2197611672389854 2316 86-87 -1.4632970297339227 2316 88-89 -1.6449125689304687 2316 90-91 -1.6377359156420788 2316 92-93 -2.0234895310566223 2316 94-95 -1.929681379848791 2316 96-97 -2.0348461367191 2316 98-99 -2.081938887429242 2316 100-101 -1.9570931876991615 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 23.0 4 42.0 5 87.0 6 180.0 7 519.0 8 1195.0 9 2134.0 10 3243.0 11 4966.0 12 7231.0 13 10521.0 14 13733.0 15 17699.0 16 21889.0 17 26292.0 18 27401.0 19 26749.0 20 28352.0 21 31190.0 22 34445.0 23 38680.0 24 44759.0 25 52683.0 26 62721.0 27 75301.0 28 89428.0 29 105528.0 30 124967.0 31 148901.0 32 178911.0 33 218527.0 34 274133.0 35 352433.0 36 460977.0 37 510940.0 38 228633.0 39 7193.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.26041656999496 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23.231498149018332 26.876108431301155 27.250790305910797 22.641603113769715 20-21 23.60545305664867 26.1562564479651 27.38979567278319 22.848494822603044 22-23 23.51758285644815 25.783152246374367 27.31050986989147 23.38875502728601 24-25 23.436242229472413 26.021545560798987 27.352535541636787 23.18967666809182 26-27 23.222687970797047 26.489103030115547 27.167839631250867 23.12036936783654 28-29 23.141723161351294 26.517452642682915 27.31178105583577 23.029043140130018 30-31 23.261723311745662 26.167319658907584 27.316936602780157 23.2540204265666 32-33 23.146421950802644 26.194300704465533 27.286520029975943 23.372757314755884 34-35 23.537347427978826 26.26501897752769 27.05300094918577 23.14463264530772 36-37 23.52742784190662 26.09916512524359 27.0762489239949 23.297158108854894 38-39 23.125670056800242 26.33629685795855 27.208509533812936 23.329523551428267 40-41 23.506093057464295 26.043004449242822 27.16588723068083 23.285015262612053 42-43 23.362950206191037 26.016883520045486 27.103746395465762 23.516419878297715 44-45 23.355667427980087 26.242001761874672 27.017706144363896 23.38462466578135 46-47 23.275085233011477 26.486531467307056 26.702982018549104 23.535401281132362 48-49 23.49767633512126 26.240244335148482 26.87016895640642 23.39191037332384 50-51 23.470883682599627 26.304327247596817 26.785242527190306 23.43954654261325 52-53 23.484962146674714 26.39414479140533 26.61599345915327 23.50489960276668 54-55 23.381749589914488 26.244745839787846 26.72895996436258 23.644544605935085 56-57 23.281782609650595 26.472053997251138 26.912441312883807 23.333722080214464 58-59 23.529994201477912 26.19734530415631 26.713921218073317 23.558739276292464 60-61 23.41856067343746 26.290791741283897 26.90193896014025 23.388708625138392 62-63 23.30746897382879 26.32741306028209 26.80847746264554 23.556640503243575 64-65 23.164564344456494 26.18670118704539 26.79302515500369 23.855709313494426 66-67 23.16556150434073 26.271792590410982 26.722555891803196 23.840090013445092 68-69 22.94728491590382 26.160160986462866 26.959477709816582 23.933076387816733 70-71 23.007794488932994 26.182138891410933 26.753303721503823 24.056762898152247 72-73 23.194043323533197 26.13408430592265 26.56981607420386 24.102056296340294 74-75 23.021781964993405 26.353683712847882 26.472532124035535 24.152002198123178 76-77 23.07977888144815 26.232301377634627 26.63235566530014 24.055564075617085 78-79 23.105230258882653 26.233746340799236 26.68149945228031 23.9795239480378 80-81 22.88160662533055 26.440317502950446 26.715109424770354 23.96296644694865 82-83 23.03984682736033 26.36027909381933 26.50334461693146 24.09652946188888 84-85 23.03597420793641 26.09818230028067 26.566116334700308 24.299727157082614 86-87 22.950924150583525 25.92571680935435 26.825975681346907 24.297383358715216 88-89 22.929165015357178 26.151076240959082 26.598385019320318 24.321373724363422 90-91 23.023383758698397 26.09248855040252 26.67449106109866 24.20963662980042 92-93 22.93501504898695 25.791816210204964 26.66235841002742 24.610810330780662 94-95 22.730324143373828 25.92449222684977 26.66869034838423 24.67649328139217 96-97 22.69709793969966 25.891809087603384 26.662715759015263 24.748377213681692 98-99 22.743739856472814 25.82138292286176 26.45145584715195 24.983421373513476 100-101 22.78207788772343 25.702732157594667 26.587189187391374 24.928000767290527 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 181.0 1 137.0 2 130.0 3 172.5 4 158.0 5 152.0 6 274.0 7 630.0 8 990.0 9 1172.0 10 1125.5 11 1028.0 12 1094.0 13 1192.0 14 1388.5 15 1585.5 16 1667.5 17 1792.0 18 1980.0 19 2147.0 20 2408.5 21 2760.0 22 3362.5 23 4201.0 24 5211.5 25 6624.0 26 8716.5 27 11643.0 28 14460.5 29 18186.5 30 23206.0 31 28165.0 32 33556.0 33 39360.5 34 45350.5 35 51471.0 36 58654.5 37 66236.0 38 73146.5 39 78835.0 40 85373.0 41 95921.5 42 106346.5 43 115586.5 44 124251.0 45 129965.5 46 133231.0 47 138339.5 48 142127.5 49 140455.0 50 138403.0 51 133953.5 52 127521.5 53 123861.5 54 123806.0 55 117828.0 56 105314.5 57 95519.0 58 86903.5 59 76578.5 60 63148.0 61 50529.0 62 41054.0 63 33033.5 64 27055.0 65 21615.0 66 15596.5 67 12101.0 68 9276.0 69 6115.5 70 4373.5 71 3250.5 72 2584.0 73 2105.0 74 1670.0 75 1356.0 76 915.0 77 482.0 78 264.5 79 149.0 80 78.5 81 47.5 82 30.0 83 14.0 84 5.5 85 4.5 86 4.0 87 3.0 88 1.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.87760536458322E-4 2 0.05104236237664376 3 0.0 4 6.186953015350757E-5 5 0.04426764882483468 6 3.093476507675379E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00627975731058102 26-27 0.0018251511395284737 28-29 1.2373906030701515E-4 30-31 0.0016704773141447047 32-33 5.104236237664376E-4 34-35 0.011291189253015133 36-37 0.02310826951233508 38-39 0.004346334493283908 40-41 0.0011291189253015135 42-43 0.0015003361062225589 44-45 0.007934767242187349 46-47 0.0 48-49 0.0 50-51 7.114995967653372E-4 52-53 0.0 54-55 0.0015931404014528203 56-57 0.0 58-59 0.023232008572642095 60-61 0.0 62-63 0.009203092610334253 64-65 0.015730328041529303 66-67 0.00627975731058102 68-69 0.016581034081140034 70-71 1.3920644284539207E-4 72-73 3.093476507675379E-5 74-75 0.023541356223409634 76-77 0.05173839459087072 78-79 0.031831873263979654 80-81 0.027562875683387632 82-83 0.03781775030633151 84-85 0.01637995810814113 86-87 0.009790853146792575 88-89 0.08813314570367155 90-91 0.025861463604166173 92-93 0.025675855013705645 94-95 0.027083386824697946 96-97 0.03972023835855187 98-99 0.055450566400081176 100-101 0.014121720257538106 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3232609.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.74580636500953 #Duplication Level Percentage of deduplicated Percentage of total 1 72.86678228422964 34.79083277384135 2 14.194197386935183 13.554267998866631 3 5.040221536936836 7.219483246180106 4 2.4223121397579743 4.626209855219846 5 1.3736005335976698 3.2791832550014055 6 0.8602732406319228 2.4644663740926624 7 0.5807734157428024 1.9410646535000713 8 0.4245375336030188 1.621590949927077 9 0.3089449347686953 1.327574252962496 >10 1.7010918976401341 14.90946489476288 >50 0.13800482837156203 4.594971720832247 >100 0.08445820747294196 7.4290445572678845 >500 0.0036988842944567805 1.184129168201734 >1k 0.0011031760170914185 1.057716299343623 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4586 0.1418668326419929 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3664 0.1133449792412259 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.5467382538376897E-5 0.0 14-15 0.0 0.0 0.0 3.0934765076753794E-5 0.0 16-17 0.0 0.0 0.0 3.0934765076753794E-5 1.5467382538376897E-5 18-19 0.0 0.0 0.0 6.186953015350759E-5 3.0934765076753794E-5 20-21 0.0 0.0 0.0 7.733691269188448E-5 3.0934765076753794E-5 22-23 0.0 0.0 0.0 1.5467382538376896E-4 3.0934765076753794E-5 24-25 0.0 0.0 0.0 2.7841288569078413E-4 3.0934765076753794E-5 26-27 0.0 0.0 0.0 5.413583888431914E-4 3.0934765076753794E-5 28-29 0.0 0.0 0.0 0.0021809009379111424 3.0934765076753794E-5 30-31 0.0 0.0 0.0 0.009110288315103993 3.0934765076753794E-5 32-33 0.0 0.0 0.0 0.02507262709470895 3.0934765076753794E-5 34-35 0.0 0.0 0.0 0.048815059291117485 3.0934765076753794E-5 36-37 0.0 0.0 0.0 0.09317551241118242 3.0934765076753794E-5 38-39 0.0 0.0 0.0 0.15891188819928423 3.0934765076753794E-5 40-41 0.0 0.0 0.0 0.2309589560630438 3.0934765076753794E-5 42-43 0.0 0.0 0.0 0.30780091251370023 3.0934765076753794E-5 44-45 0.0 0.0 0.0 0.39791388318228404 3.0934765076753794E-5 46-47 0.0 0.0 0.0 0.5102225477934387 3.0934765076753794E-5 48-49 0.0 0.0 0.0 0.6285789589770987 3.0934765076753794E-5 50-51 0.0 0.0 0.0 0.7537719532427213 3.0934765076753794E-5 52-53 0.0 0.0 0.0 0.8794908385146487 3.0934765076753794E-5 54-55 0.0 0.0 0.0 1.019687193842497 3.0934765076753794E-5 56-57 0.0 0.0 0.0 1.1789084296925485 3.0934765076753794E-5 58-59 0.0 0.0 0.0 1.3558552859315802 3.0934765076753794E-5 60-61 0.0 0.0 0.0 1.546784655985305 3.0934765076753794E-5 62-63 0.0 0.0 0.0 1.7446743481812987 3.0934765076753794E-5 64-65 0.0 0.0 0.0 1.9528962519129285 3.0934765076753794E-5 66-67 0.0 0.0 0.0 2.188217009851795 6.186953015350759E-5 68-69 0.0 0.0 0.0 2.4508995675010494 6.186953015350759E-5 70-71 0.0 0.0 0.0 2.7339990701009618 6.186953015350759E-5 72-73 0.0 0.0 0.0 3.0194032126990926 6.186953015350759E-5 74-75 0.0 0.0 0.0 3.315974805489931 6.186953015350759E-5 76-77 0.0 0.0 0.0 3.6439606522162133 6.186953015350759E-5 78-79 0.0 0.0 0.0 3.999756852746497 6.186953015350759E-5 80-81 0.0 0.0 0.0 4.387462263453452 6.186953015350759E-5 82-83 0.0 0.0 0.0 4.7854070814008125 6.186953015350759E-5 84-85 0.0 0.0 0.0 5.183908725119555 6.186953015350759E-5 86-87 0.0 0.0 0.0 5.6004144021129685 6.186953015350759E-5 88-89 0.0 0.0 0.0 6.058156120953694 6.186953015350759E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4685 0.0 37.32989 1 GTATCAA 6665 0.0 36.864548 1 ATCAACG 8115 0.0 29.443968 3 TCAACGC 8325 0.0 28.815357 4 TATCAAC 8510 0.0 28.369127 2 CAACGCA 8535 0.0 28.05071 5 AACGCAG 8885 0.0 27.042198 6 GTACATG 9275 0.0 24.748665 1 TACATGG 9530 0.0 23.687132 2 ACGCAGA 10540 0.0 22.570753 7 ACATGGG 9575 0.0 22.47379 3 CGCAGAG 10715 0.0 22.157806 8 ATTCGCG 655 0.0 20.309574 80-81 CGCGGAA 660 0.0 20.163828 82-83 CATGGGG 6775 0.0 19.071133 4 GCAGAGT 12155 0.0 19.024923 9 TATACCG 300 3.7198333E-9 19.001017 5 GAGTACT 5620 0.0 18.545868 12-13 TCGCGGA 730 0.0 17.904768 82-83 AGTACTT 5990 0.0 17.836292 12-13 >>END_MODULE