FastQCFastQC Report
Thu 26 May 2016
SRR938538_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938538_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255714
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47230.14506802501693944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35110.10784116786671065No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65700.038.8410381
GGTATCA44350.036.2839431
ATCAACG80900.031.3137473
TCAACGC82300.030.7233184
CAACGCA84050.030.081785
TATCAAC85800.029.3038522
AACGCAG88800.028.519186
GTACATG91800.027.6942751
TACATGG95850.025.9833722
ACGCAGA103200.024.3526027
ACATGGG97700.024.1292293
CGCAGAG107250.023.5215878
CATGGGG71500.021.6039734
ACCGTGC7450.020.4062468
GCAGAGT118850.020.3857679
GAGTACT54700.018.84466612-13
GCACCGT11400.018.7555776
ACCGAGT10650.018.7356648
AGGACCG13350.018.511935
AGTACTT60200.018.26713812-13
Produced by FastQC (version 0.11.5)