FastQCFastQC Report
Thu 26 May 2016
SRR938538_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938538_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3255714
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45120.1385871117671884No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38470.11816148470043746No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44750.039.643451
GTATCAA65150.037.1585771
ATCAACG80600.029.4915083
TATCAAC83050.028.920612
TCAACGC82400.028.8468364
CAACGCA84400.028.1632585
AACGCAG86950.027.3117036
GTACATG90250.025.6120761
TACATGG92300.024.2702872
GGACCGA10900.023.965396
ACGCAGA103500.022.852697
ACATGGG95950.022.6925133
AGGACCG13100.022.4996515
ACCGAGT11000.022.4522048
CGCAGAG105600.022.3532548
GCAGAGT116700.019.738739
GAGTACT55250.019.64274812-13
CATGGGG68700.019.5832634
GGACCGT4250.018.998026
ATTCGCG7150.018.60117380-81
Produced by FastQC (version 0.11.5)