##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938538_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3255714 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79274070142525 31.0 31.0 34.0 30.0 34.0 2 32.02724072200445 31.0 31.0 34.0 30.0 34.0 3 32.09940891613944 33.0 31.0 34.0 30.0 34.0 4 35.75826408585029 37.0 35.0 37.0 35.0 37.0 5 35.61907219123056 37.0 35.0 37.0 33.0 37.0 6 35.64709400150013 37.0 35.0 37.0 33.0 37.0 7 35.58327021353841 37.0 35.0 37.0 33.0 37.0 8 35.57538162135863 37.0 35.0 37.0 33.0 37.0 9 37.16245745172948 39.0 37.0 39.0 33.0 39.0 10-11 37.06526893947073 39.0 37.0 39.0 33.0 39.0 12-13 36.95229602477367 39.0 37.0 39.0 33.0 39.0 14-15 38.03409758965314 40.0 37.0 41.0 33.0 41.0 16-17 37.95426840318284 40.0 37.0 41.0 33.0 41.0 18-19 38.03176154293651 40.0 37.0 41.0 33.0 41.0 20-21 38.076054131290405 40.0 37.0 41.0 33.0 41.0 22-23 37.97906188934286 40.0 37.0 41.0 33.0 41.0 24-25 37.88234285935435 40.0 37.0 41.0 32.0 41.0 26-27 37.70622619185838 40.0 37.0 41.0 32.0 41.0 28-29 37.58524289909986 39.5 37.0 41.0 32.0 41.0 30-31 37.39294744562943 39.0 36.0 41.0 31.5 41.0 32-33 37.22188558331598 39.0 36.0 41.0 31.0 41.0 34-35 37.095251917091 39.0 36.0 41.0 31.0 41.0 36-37 37.135713241396516 39.0 36.0 41.0 31.0 41.0 38-39 37.04446336502531 39.0 36.0 41.0 31.0 41.0 40-41 36.89836284759656 39.0 36.0 41.0 30.0 41.0 42-43 36.72093709705459 39.0 35.0 41.0 30.0 41.0 44-45 36.50325919291436 39.0 35.0 40.0 30.0 41.0 46-47 36.20045587542395 38.5 35.0 40.0 29.5 41.0 48-49 35.91179507782317 38.0 35.0 40.0 29.0 41.0 50-51 35.57443881741455 38.0 34.0 40.0 28.0 41.0 52-53 35.09550531772754 38.0 34.0 40.0 26.5 41.0 54-55 34.7832476992758 38.0 33.0 40.0 26.0 41.0 56-57 34.454169500146506 37.5 33.0 40.0 26.0 41.0 58-59 34.00785127317695 37.0 33.0 40.0 24.5 41.0 60-61 33.63413862519865 36.5 32.0 39.0 23.5 40.5 62-63 33.16749275274179 36.0 31.5 39.0 22.5 40.0 64-65 32.654632747225335 35.5 31.0 39.0 22.0 40.0 66-67 32.15078766132406 35.0 31.0 38.0 20.5 40.0 68-69 31.61098471794513 35.0 30.0 38.0 19.5 40.0 70-71 31.031256584577147 34.0 29.5 37.0 18.0 39.0 72-73 30.57391389415655 34.0 29.0 36.5 16.0 39.0 74-75 30.03558604963458 34.0 29.0 36.0 12.0 39.0 76-77 28.75544765295723 32.5 27.0 34.5 11.0 36.5 78-79 29.457092975611495 33.5 29.0 35.0 8.0 37.0 80-81 29.17256015116807 33.5 29.0 35.0 7.0 37.0 82-83 28.700099118042925 33.0 27.5 35.0 4.0 36.0 84-85 28.167828009462745 33.0 27.0 35.0 2.0 36.0 86-87 27.57788368388624 32.5 26.0 35.0 2.0 35.0 88-89 26.937914693981106 32.0 25.0 34.0 2.0 35.0 90-91 26.386664031300047 32.0 24.5 34.0 2.0 35.0 92-93 25.620081186492428 31.0 23.0 34.0 2.0 35.0 94-95 24.837979318822228 31.0 19.0 34.0 2.0 35.0 96-97 23.821114354639256 31.0 8.5 34.0 2.0 35.0 98-99 22.54964855635354 30.5 2.0 34.0 2.0 35.0 100-101 18.851535331420386 24.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3255391159043022 1101 2 0.3392078536613852 1101 3 0.3407605171345054 1101 4 0.19838519860995518 1101 5 0.03354368205049241 1101 6 0.08612256082629699 1101 7 0.2663284557409966 1101 8 0.22567609087825957 1101 9 0.32598139774434287 1101 10-11 0.3004057020840065 1101 12-13 0.34144131668316646 1101 14-15 0.39886875347315964 1101 16-17 0.44586666628525506 1101 18-19 0.4280523205161799 1101 20-21 0.4004018977282229 1101 22-23 0.40300012665134943 1101 24-25 0.49456268796692626 1101 26-27 0.4470398187775686 1101 28-29 0.32736385863286444 1101 30-31 0.34728256707011695 1101 32-33 0.16931823854646666 1101 34-35 0.09456793962147714 1101 36-37 0.28216793642940985 1101 38-39 0.3041292671481699 1101 40-41 0.33637074300940384 1101 42-43 0.39348762729955666 1101 44-45 0.35703820585121804 1101 46-47 0.40719183369007794 1101 48-49 0.24267918747095507 1101 50-51 0.4174894495826038 1101 52-53 0.6027564758307875 1101 54-55 0.4461274343945476 1101 56-57 0.3358098105285805 1101 58-59 0.4623652884146807 1101 60-61 0.5323727393909223 1101 62-63 0.5342854435706457 1101 64-65 0.46292157055046346 1101 66-67 0.31845388575218436 1101 68-69 0.29831104210977344 1101 70-71 0.1705330906834348 1101 72-73 0.2579694914288275 1101 74-75 0.39285805715950417 1101 76-77 0.3901511937792499 1101 78-79 0.39802862369582215 1101 80-81 0.34275527010221296 1101 82-83 0.3247152426960689 1101 84-85 0.38528850638688183 1101 86-87 0.47129832394784543 1101 88-89 0.44442751369639666 1101 90-91 0.41232373236097786 1101 92-93 0.2987361401529611 1101 94-95 0.4277171147090151 1101 96-97 0.7188003129901404 1101 98-99 0.5357399044950206 1101 100-101 0.2006192190933973 1102 1 0.2723472980363404 1102 2 0.2039375134105157 1102 3 0.24057782888409207 1102 4 0.20179672187513376 1102 5 0.11699279785854344 1102 6 0.17620129418776287 1102 7 0.24775620517519314 1102 8 0.22711736211996936 1102 9 0.2792770746019144 1102 10-11 0.37666428507530725 1102 12-13 0.3658618928464179 1102 14-15 0.47331646915078807 1102 16-17 0.38356877415283464 1102 18-19 0.3732217267074489 1102 20-21 0.37247188506950835 1102 22-23 0.390800531346585 1102 24-25 0.33667937828876404 1102 26-27 0.33927844511536875 1102 28-29 0.19629376580229518 1102 30-31 0.29784981169016334 1102 32-33 0.2674915478410327 1102 34-35 0.26878669561519075 1102 36-37 0.3710324402462035 1102 38-39 0.42382104665640696 1102 40-41 0.45663053433242595 1102 42-43 0.4670919561959437 1102 44-45 0.43854435299916616 1102 46-47 0.16539381688851051 1102 48-49 0.4634326686171448 1102 50-51 0.4062791998607054 1102 52-53 0.28935473429869774 1102 54-55 0.3571747990805676 1102 56-57 0.3210812056722361 1102 58-59 0.32089577481615805 1102 60-61 0.4348359140428073 1102 62-63 0.3215161582125532 1102 64-65 0.32673754929917465 1102 66-67 0.37720239931234545 1102 68-69 0.3609990048241052 1102 70-71 0.13293071813366453 1102 72-73 0.03305498569404719 1102 74-75 0.15287186666504837 1102 76-77 0.1750747140523714 1102 78-79 0.3061127945500921 1102 80-81 0.40222744011257205 1102 82-83 0.35357010862878724 1102 84-85 0.18959347589089148 1102 86-87 0.11307773627666862 1102 88-89 0.22007526581576897 1102 90-91 0.04234511791470297 1102 92-93 0.2161763469119471 1102 94-95 0.14573017619967388 1102 96-97 0.21181649120948265 1102 98-99 0.05788114504893471 1102 100-101 -0.016005028096753193 1103 1 0.2807844213790496 1103 2 0.27420095828223623 1103 3 0.283578460233457 1103 4 0.1498033797411722 1103 5 0.21130032239507557 1103 6 0.19371607350245768 1103 7 0.25909983141636417 1103 8 0.2798132239118516 1103 9 0.358730670423526 1103 10-11 0.323939725584971 1103 12-13 0.3759990678449867 1103 14-15 0.5073760455727552 1103 16-17 0.48336444653990895 1103 18-19 0.48371819689054263 1103 20-21 0.3619490130038514 1103 22-23 0.46123042286928495 1103 24-25 0.46440135498623647 1103 26-27 0.4898093525365965 1103 28-29 0.49917616263407183 1103 30-31 0.5689753354231684 1103 32-33 0.5647457520538524 1103 34-35 0.588045147087378 1103 36-37 0.6777224477076018 1103 38-39 0.6430772023127176 1103 40-41 0.6758534006585535 1103 42-43 0.7400633158928969 1103 44-45 0.6970497768641692 1103 46-47 0.7101174580524869 1103 48-49 0.7563202310346426 1103 50-51 0.7387053032872117 1103 52-53 0.7906897512303459 1103 54-55 0.8848910362383506 1103 56-57 0.729628528176967 1103 58-59 0.7983164256799569 1103 60-61 0.7591807455873223 1103 62-63 0.6489362351223846 1103 64-65 0.5615157160901987 1103 66-67 0.69990342668995 1103 68-69 0.6022777742667991 1103 70-71 0.41316603926091133 1103 72-73 0.2092909955660538 1103 74-75 0.39862977288096957 1103 76-77 0.39302340256755386 1103 78-79 0.5188947494373117 1103 80-81 0.5310697749573201 1103 82-83 0.4640987202341371 1103 84-85 0.3424179033718069 1103 86-87 0.4797447855793102 1103 88-89 0.5147811144067127 1103 90-91 0.478304481973705 1103 92-93 0.48108250543803166 1103 94-95 0.2736874740243884 1103 96-97 0.3343189305138523 1103 98-99 -0.042456339884772376 1103 100-101 -0.31296307206162766 1104 1 0.27405809307314755 1104 2 0.2728299624215964 1104 3 0.2128427624873126 1104 4 0.1327650347705429 1104 5 0.1482529298351949 1104 6 0.23370462991131546 1104 7 0.18251163083166944 1104 8 0.25481666592949637 1104 9 0.20471289709603724 1104 10-11 0.3122111622026793 1104 12-13 0.28452624190379794 1104 14-15 0.43022836066488424 1104 16-17 0.40030797956986675 1104 18-19 0.4053927608271337 1104 20-21 0.353289477720935 1104 22-23 0.39715882890220655 1104 24-25 0.3655832186823318 1104 26-27 0.47957784332361086 1104 28-29 0.4679137020831732 1104 30-31 0.41243554659317994 1104 32-33 0.36010421360607126 1104 34-35 0.35273768997849686 1104 36-37 0.4153055701987327 1104 38-39 0.45339820398455544 1104 40-41 0.4027038204058968 1104 42-43 0.5233377922145053 1104 44-45 0.42721687117545315 1104 46-47 0.4342186891325639 1104 48-49 0.5642308369191511 1104 50-51 0.5790597864034481 1104 52-53 0.5494175457463655 1104 54-55 0.5352332264344071 1104 56-57 0.5364817194190437 1104 58-59 0.5204094853207337 1104 60-61 0.5054556778645036 1104 62-63 0.5040297507187503 1104 64-65 0.5781657835895047 1104 66-67 0.4450014571406342 1104 68-69 0.19557343911262492 1104 70-71 0.36064853861273605 1104 72-73 0.200139654073201 1104 74-75 0.04934575725331314 1104 76-77 -0.024970079949678592 1104 78-79 0.01861576720169822 1104 80-81 0.12918444331668155 1104 82-83 0.16018532497428595 1104 84-85 0.22960577495325651 1104 86-87 0.2070964435589815 1104 88-89 0.06964915549636075 1104 90-91 0.1791897957337305 1104 92-93 0.34304825512735704 1104 94-95 0.5157049880830158 1104 96-97 0.5711478488262784 1104 98-99 0.4537380402391413 1104 100-101 0.14420419211546331 1105 1 0.2439392561403686 1105 2 0.2508752020311391 1105 3 0.20680586012854718 1105 4 0.1547045862458063 1105 5 0.12129201674723333 1105 6 0.18680621163849764 1105 7 0.19943293160870468 1105 8 0.1822642643035266 1105 9 0.18887142929745693 1105 10-11 0.21533573013122265 1105 12-13 0.2722224023440276 1105 14-15 0.35098178958394044 1105 16-17 0.23384726696305336 1105 18-19 0.28809745899929595 1105 20-21 0.27342763688914573 1105 22-23 0.3460452943503043 1105 24-25 0.38283617750765586 1105 26-27 0.34188030025472926 1105 28-29 0.36884524813853403 1105 30-31 0.3844122562135155 1105 32-33 0.40786124427277315 1105 34-35 0.41162488130665054 1105 36-37 0.5012086341038255 1105 38-39 0.30822167687120583 1105 40-41 0.3505616101357347 1105 42-43 0.3992803992990872 1105 44-45 0.3126724818825366 1105 46-47 -0.043972417799935215 1105 48-49 -0.06367233759623758 1105 50-51 -0.0922686432069284 1105 52-53 -0.010965476956400266 1105 54-55 0.04478131425897658 1105 56-57 0.021513035958044213 1105 58-59 0.08724696423565348 1105 60-61 0.02289511872947969 1105 62-63 0.28300359829776056 1105 64-65 0.22675308276269135 1105 66-67 0.07365211527186943 1105 68-69 -0.06314887374858813 1105 70-71 -0.11188554318357191 1105 72-73 0.02286592125236453 1105 74-75 0.019231932349406122 1105 76-77 -0.009622383409016777 1105 78-79 0.05250814338730336 1105 80-81 0.1299229398462849 1105 82-83 0.198606501314746 1105 84-85 0.1643655777904094 1105 86-87 0.1108551195537828 1105 88-89 -0.04274161684000788 1105 90-91 -6.01358208147218E-4 1105 92-93 0.04304635401295798 1105 94-95 0.2129718768920199 1105 96-97 0.09764730960110413 1105 98-99 0.06976979230617175 1105 100-101 -0.19165099827250387 1106 1 0.0625846464071742 1106 2 0.06523331947023792 1106 3 0.08954974235800961 1106 4 0.059857047163887955 1106 5 -0.0019962503908104168 1106 6 -0.060384171170980494 1106 7 0.028995711258488654 1106 8 0.009776969380034473 1106 9 -0.036809397084908824 1106 10-11 0.0301485374007342 1106 12-13 0.04389733191371192 1106 14-15 0.09639587840900532 1106 16-17 -0.16839787196418854 1106 18-19 0.09415860908492135 1106 20-21 0.1240073060047635 1106 22-23 0.22985150050398317 1106 24-25 0.09049961726901756 1106 26-27 0.039637232807336886 1106 28-29 0.20538408148107123 1106 30-31 0.2588788501482213 1106 32-33 0.22714672181793105 1106 34-35 0.09361267338531576 1106 36-37 -0.36621389254921866 1106 38-39 -0.07107215564273872 1106 40-41 0.13306278397432436 1106 42-43 0.19838690098389122 1106 44-45 0.1584483891907169 1106 46-47 -0.07275839250026195 1106 48-49 0.045580272468392025 1106 50-51 0.1350141276045349 1106 52-53 0.06441602058544049 1106 54-55 -0.1990719144876394 1106 56-57 0.08372993370979742 1106 58-59 0.07088330904037576 1106 60-61 -0.19912471223776151 1106 62-63 -0.2461473021054772 1106 64-65 -0.577515546746227 1106 66-67 -0.24452132283231265 1106 68-69 -0.899336936500621 1106 70-71 -0.8378218520826479 1106 72-73 -0.375742044278212 1106 74-75 -0.20192927021370366 1106 76-77 -0.3194788298543578 1106 78-79 -0.39509860687508436 1106 80-81 -1.2077085991251586 1106 82-83 -0.5826548918245145 1106 84-85 -0.42643601586809865 1106 86-87 -0.30985208367220096 1106 88-89 -0.2591829525302245 1106 90-91 -0.39288923866994807 1106 92-93 -0.43422088069952025 1106 94-95 -0.5287912286705954 1106 96-97 -0.5991287772353324 1106 98-99 -0.636794382140053 1106 100-101 -0.940413289053037 1107 1 0.1483475071757283 1107 2 0.1682374192977889 1107 3 0.11958328393654938 1107 4 0.13636275763796846 1107 5 0.08764409277993934 1107 6 0.11892498619514669 1107 7 0.09819182509920665 1107 8 0.15399491100630058 1107 9 0.17484758943338363 1107 10-11 0.1913372433519953 1107 12-13 0.1584315188354779 1107 14-15 0.26493926011596614 1107 16-17 0.22877664999374758 1107 18-19 0.2864481810976329 1107 20-21 0.2801495228400839 1107 22-23 0.2910835230855753 1107 24-25 0.21750818026862362 1107 26-27 0.2617698616861759 1107 28-29 0.3045313742933615 1107 30-31 0.29920398924951286 1107 32-33 0.38235617365580765 1107 34-35 0.2603698372374126 1107 36-37 0.17372044784493568 1107 38-39 0.1258964936918474 1107 40-41 0.17845000346694206 1107 42-43 0.19080038974190927 1107 44-45 0.1544061815745792 1107 46-47 0.15669061182733657 1107 48-49 0.19107771549922603 1107 50-51 0.2648182740703362 1107 52-53 0.18698039221636975 1107 54-55 0.26628630523274666 1107 56-57 0.20261171388095534 1107 58-59 0.07701745908538982 1107 60-61 0.03700922088515313 1107 62-63 0.10623551929170816 1107 64-65 0.26617513861911846 1107 66-67 0.24438921307371686 1107 68-69 0.27889676180413403 1107 70-71 0.09618231282152578 1107 72-73 0.004524756337847435 1107 74-75 0.037844547832044384 1107 76-77 0.07272071948999326 1107 78-79 0.2050739817647056 1107 80-81 0.3018580040917982 1107 82-83 0.014921410521498757 1107 84-85 0.2706427892165557 1107 86-87 0.47738971124965346 1107 88-89 0.41647310122307246 1107 90-91 0.4182946543223842 1107 92-93 0.20421100305193107 1107 94-95 -0.14602846822850424 1107 96-97 -0.12582951422814403 1107 98-99 -0.036975986604307565 1107 100-101 -0.11341814810790751 1108 1 0.22099175790217274 1108 2 0.20198107290445222 1108 3 0.1859589744560779 1108 4 0.10204832777559147 1108 5 0.11309352918827642 1108 6 0.12987351255463153 1108 7 0.18026585837186815 1108 8 0.11058797547607924 1108 9 0.13723024102836234 1108 10-11 0.21262560111295414 1108 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-0.7402244611603024 2316 58-59 -0.8012978096680996 2316 60-61 -0.6627302349279489 2316 62-63 -0.7886353899450924 2316 64-65 -0.5171169424561413 2316 66-67 -0.43837603585948415 2316 68-69 -0.49107907836342335 2316 70-71 -0.6127151099925143 2316 72-73 -0.4726273479045169 2316 74-75 -0.05531778751898031 2316 76-77 -0.12832758680133693 2316 78-79 -0.1311176049692797 2316 80-81 -0.2892256015132979 2316 82-83 -0.0892510856413189 2316 84-85 -0.1359539800766747 2316 86-87 -0.01354846557199707 2316 88-89 0.07206946896689104 2316 90-91 -0.027540710761815745 2316 92-93 0.10532564558036839 2316 94-95 0.32148104928124255 2316 96-97 0.49591569804096025 2316 98-99 0.41986615304024255 2316 100-101 0.5261288451751405 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 16.0 4 54.0 5 87.0 6 190.0 7 487.0 8 1201.0 9 1947.0 10 3231.0 11 4765.0 12 7020.0 13 9974.0 14 13200.0 15 16847.0 16 20516.0 17 24924.0 18 25724.0 19 24701.0 20 25974.0 21 28604.0 22 31419.0 23 35335.0 24 41506.0 25 48956.0 26 59101.0 27 71123.0 28 85752.0 29 102595.0 30 121294.0 31 143910.0 32 174014.0 33 214579.0 34 270885.0 35 352254.0 36 471990.0 37 544004.0 38 267479.0 39 10052.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28189609840722 18.925845656351207 14.55089787252864 24.24136037271293 2 16.26785201210134 19.928123324273205 38.767218212855134 25.036806450770317 3 19.654333243541515 22.935799260307913 29.14617810908262 28.263689387067952 4 13.79764930211929 16.283770626043932 35.411587135725064 34.50699293611171 5 14.695440772840405 36.45420359858733 33.88435062124328 14.966005007328997 6 34.18214867767869 35.007251865489415 16.6692467458751 14.141352710956797 7 30.423280423280424 31.102977718558815 19.748601996367 18.725139861793757 8 26.727378387659357 35.61218829418063 18.8973294337279 18.76310388443211 9 26.263486289029075 16.645872456855855 19.36515308162818 37.72548817248689 10-11 25.71730809278702 26.04694392689284 26.91865747421303 21.31709050610711 12-13 26.086843008937517 22.876625526689384 27.445807586292897 23.5907238780802 14-15 22.79616698518359 24.726035517861828 25.085680130380002 27.39211736657458 16-17 23.357503146775176 28.054736994711455 25.6452655239373 22.94249433457607 18-19 23.227547014264765 26.874181823096254 27.232321389409513 22.665949773229464 20-21 23.602518525890172 26.13867495732119 27.406584239279002 22.852222277509636 22-23 23.52820303011874 25.814782870976998 27.299818104415806 23.357195994488457 24-25 23.42374385885699 26.047000572840602 27.34608940306324 23.18316616523917 26-27 23.219342676336304 26.460160354461937 27.21530263594819 23.105194333253568 28-29 23.07212795718543 26.501053225191157 27.401378008019133 23.025440809604284 30-31 23.24170673468247 26.18087768151625 27.323837413237158 23.253578170564122 32-33 23.130625110190884 26.193348064357004 27.271268299365364 23.40475852608675 34-35 23.55518283692126 26.220264425439886 27.085299321734002 23.139253415904847 36-37 23.5524346778028 26.08125482997861 27.09399921614592 23.272311276072667 38-39 23.125457677431406 26.328754306468184 27.215005184963115 23.330782831137302 40-41 23.51866357040614 26.060238854383606 27.14316211749817 23.277935457712083 42-43 23.350156126082794 26.03157475714915 27.141611855139075 23.476657261628983 44-45 23.321351322628463 26.269398970548398 27.03347099898824 23.3757787078349 46-47 23.307391251197128 26.47079872494943 26.708411733954517 23.51339828989893 48-49 23.478091134540687 26.221114631076315 26.907246766761457 23.39354746762154 50-51 23.474574240857766 26.30547400662343 26.759875099594126 23.460076652924673 52-53 23.493801974006317 26.42426515351164 26.616665345911834 23.46526752657021 54-55 23.370557118960694 26.262918057298645 26.722172156399488 23.644352667341174 56-57 23.272867334170016 26.48589525984162 26.933631148190535 23.30760625779783 58-59 23.509958184287687 26.226290147107516 26.711575402507716 23.55217626609708 60-61 23.44279933679678 26.285447677529415 26.924278361059972 23.34747462461383 62-63 23.28458519390831 26.340996168582375 26.838920740581024 23.53549789692829 64-65 23.148639591806898 26.180754820601564 26.832009814129865 23.838595773461673 66-67 23.165601513401214 26.29043161974044 26.7394423279939 23.804524538864452 68-69 22.985349644883158 26.16553669591905 26.948566033180686 23.900547626017104 70-71 23.020835844945108 26.218800406608512 26.759926363254237 24.000437385192146 72-73 23.208764651931958 26.14196148678907 26.614177412389417 24.035096448889554 74-75 22.98862860804112 26.36039283548862 26.536897897051155 24.114080659419102 76-77 23.080037128568417 26.24061880128291 26.646013746907744 24.03333032324092 78-79 23.13146977897936 26.206309890856506 26.726364785113187 23.935855545050945 80-81 22.887202433287694 26.43528113580046 26.727140510692475 23.950375920219372 82-83 23.011899759682723 26.383513694153258 26.538565025727216 24.0660215204368 84-85 23.035964927234808 26.082308974959716 26.594415209972926 24.287310887832547 86-87 22.94203416714737 25.981296469329244 26.816293069078235 24.26037629444515 88-89 22.906314026903182 26.19514966237027 26.61404427265745 24.284492038069097 90-91 23.018567021674272 26.13815041170459 26.654090430337043 24.189192136284095 92-93 22.87935539327218 25.869888430733816 26.69975846905595 24.550997706938052 94-95 22.733422755629874 25.97126958058035 26.704135161790983 24.591172501998795 96-97 22.698600061307598 25.945445453746856 26.648409534744143 24.7075449502014 98-99 22.723773282737124 25.82268115587023 26.493579053303712 24.95996650808894 100-101 22.763815483735822 25.774968493844515 26.571598909363324 24.889617113056342 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 158.0 2 121.0 3 159.5 4 159.5 5 155.5 6 296.0 7 658.0 8 1028.0 9 1191.5 10 1116.5 11 1032.5 12 1106.0 13 1233.0 14 1424.0 15 1635.0 16 1741.5 17 1800.5 18 1930.5 19 2134.5 20 2421.5 21 2851.5 22 3394.5 23 4113.5 24 5184.5 25 6671.0 26 8702.0 27 11493.5 28 14498.0 29 18235.0 30 23178.0 31 28257.5 32 33849.0 33 39657.0 34 45580.5 35 51418.5 36 58613.5 37 66934.5 38 74286.0 39 79752.5 40 86002.5 41 96812.0 42 107515.5 43 116058.0 44 125075.5 45 131721.0 46 134666.5 47 139620.5 48 143920.0 49 142263.5 50 139354.5 51 134891.0 52 128941.5 53 125328.0 54 125226.5 55 118722.0 56 105535.0 57 95968.5 58 87380.0 59 76390.5 60 62735.0 61 50597.5 62 41372.0 63 33227.5 64 27082.0 65 21380.5 66 15398.5 67 11991.0 68 9083.5 69 6011.5 70 4355.5 71 3249.0 72 2595.0 73 2075.0 74 1618.5 75 1289.5 76 867.5 77 495.0 78 273.5 79 153.5 80 87.0 81 49.5 82 24.0 83 15.0 84 11.0 85 5.0 86 1.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.528741160925069E-4 2 0.04696358463919128 3 0.001075033003513208 4 0.0 5 0.0885212890321447 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.7643705804625344E-4 26-27 0.009245283830213589 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010873190949819303 36-37 1.8429137203083564E-4 38-39 0.004484423386083666 40-41 0.005344449788894233 42-43 3.685827440616713E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.071522867180594E-5 68-69 7.678807167951484E-4 70-71 7.678807167951485E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0011211058465209166 82-83 5.528741160925069E-4 84-85 0.004284774399716929 86-87 0.0022114964643700275 88-89 7.678807167951485E-5 90-91 0.0031636685531960117 92-93 7.218078737874395E-4 94-95 3.071522867180594E-5 96-97 0.0 98-99 7.678807167951485E-5 100-101 3.071522867180594E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3255714.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.4505724482525 #Duplication Level Percentage of deduplicated Percentage of total 1 72.81282656224354 34.550103019537815 2 13.950069431642682 13.238775604486271 3 5.2464864358002234 7.468463541621377 4 2.4449623205103377 4.640594468904934 5 1.363441938346793 3.2348050237255164 6 0.884147370237811 2.5171979307840706 7 0.5929736156052013 1.9695856255023785 8 0.42630002984713367 1.6182544360762892 9 0.317941678001667 1.3577863163703363 >10 1.731667179885748 15.12301088535691 >50 0.14117763051724358 4.672030266033495 >100 0.08327289367576861 7.394700962150698 >500 0.003695562741428869 1.2007925518674218 >1k 0.0010373509443975504 1.0138993675825567 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4512 0.1385871117671884 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3847 0.11816148470043746 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 7.678807167951485E-5 0.0 22-23 0.0 0.0 0.0 1.2286091468722375E-4 0.0 24-25 0.0 0.0 0.0 3.071522867180594E-4 0.0 26-27 0.0 0.0 0.0 6.296621877720217E-4 0.0 28-29 0.0 0.0 0.0 0.002165423621362319 0.0 30-31 0.0 0.0 0.0 0.009076350072518655 0.0 32-33 0.0 0.0 0.0 0.02463361339478836 0.0 34-35 0.0 0.0 0.0 0.04920579633223311 0.0 36-37 0.0 0.0 0.0 0.09299035480389248 0.0 38-39 0.0 0.0 0.0 0.16143924189901201 0.0 40-41 0.0 0.0 0.0 0.23398861202181764 0.0 42-43 0.0 0.0 0.0 0.307213717175403 0.0 44-45 0.0 0.0 0.0 0.39896010521808734 0.0 46-47 0.0 0.0 0.0 0.509012769549168 0.0 48-49 0.0 0.0 0.0 0.6260685060174205 0.0 50-51 0.0 0.0 0.0 0.7534292017050638 0.0 52-53 0.0 0.0 0.0 0.8810049039934098 0.0 54-55 0.0 0.0 0.0 1.0219724459826631 0.0 56-57 0.0 0.0 0.0 1.188971144271272 0.0 58-59 0.0 0.0 0.0 1.3708513708513708 0.0 60-61 0.0 0.0 0.0 1.5628829805075015 3.071522867180594E-5 62-63 0.0 0.0 0.0 1.757632887901087 3.071522867180594E-5 64-65 0.0 0.0 0.0 1.966573230941047 3.071522867180594E-5 66-67 0.0 0.0 0.0 2.2063516635675002 3.071522867180594E-5 68-69 0.0 0.0 0.0 2.4703183387730006 3.071522867180594E-5 70-71 0.0 0.0 0.0 2.7548642171886106 3.071522867180594E-5 72-73 0.0 0.0 0.0 3.040331552464375 3.071522867180594E-5 74-75 0.0 0.0 0.0 3.343060846253694 3.071522867180594E-5 76-77 0.0 0.0 0.0 3.669502296577648 3.071522867180594E-5 78-79 0.0 3.071522867180594E-5 0.0 4.03208021343398 3.071522867180594E-5 80-81 0.0 3.071522867180594E-5 0.0 4.419322458913774 3.071522867180594E-5 82-83 0.0 3.071522867180594E-5 0.0 4.817146100671005 3.071522867180594E-5 84-85 0.0 3.071522867180594E-5 0.0 5.217181238892605 3.071522867180594E-5 86-87 0.0 3.071522867180594E-5 0.0 5.639008831856852 3.071522867180594E-5 88-89 0.0 3.071522867180594E-5 0.0 6.099430109647224 3.071522867180594E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4475 0.0 39.64345 1 GTATCAA 6515 0.0 37.158577 1 ATCAACG 8060 0.0 29.491508 3 TATCAAC 8305 0.0 28.92061 2 TCAACGC 8240 0.0 28.846836 4 CAACGCA 8440 0.0 28.163258 5 AACGCAG 8695 0.0 27.311703 6 GTACATG 9025 0.0 25.612076 1 TACATGG 9230 0.0 24.270287 2 GGACCGA 1090 0.0 23.96539 6 ACGCAGA 10350 0.0 22.85269 7 ACATGGG 9595 0.0 22.692513 3 AGGACCG 1310 0.0 22.499651 5 ACCGAGT 1100 0.0 22.452204 8 CGCAGAG 10560 0.0 22.353254 8 GCAGAGT 11670 0.0 19.73873 9 GAGTACT 5525 0.0 19.642748 12-13 CATGGGG 6870 0.0 19.583263 4 GGACCGT 425 0.0 18.99802 6 ATTCGCG 715 0.0 18.601173 80-81 >>END_MODULE