FastQCFastQC Report
Thu 26 May 2016
SRR938537_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938537_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268056
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47030.14390818272391906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.10847427339066407No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA65200.040.8655051
GGTATCA41750.038.1313861
ATCAACG80350.032.886883
TCAACGC82500.031.972224
CAACGCA84050.031.4391525
TATCAAC86200.030.875542
AACGCAG88250.030.1532336
GTACATG92150.026.4830041
ACGCAGA102800.025.5544347
CGCAGAG104500.025.0932568
TACATGG95000.024.96382
ACATGGG99700.022.8335863
GCAGAGT117600.021.3685959
ACCGTGC6200.019.9212198
GAGTACT53750.019.88375712-13
GGACCGT3850.019.7478526
AGTACTT56750.019.5859412-13
AGAGTAC106350.019.4511410-11
CATGGGG76350.018.8611134
ATTCGCG6450.018.77507880-81
Produced by FastQC (version 0.11.5)