FastQCFastQC Report
Thu 26 May 2016
SRR938537_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938537_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3268056
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46750.1430514042599025No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38270.1171032564925448No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45300.039.9042781
GTATCAA65800.037.8102761
ATCAACG81950.029.5295433
TCAACGC83900.028.9565494
TATCAAC84750.028.7385142
CAACGCA85450.028.375665
AACGCAG88800.027.4380656
TATACCG1806.6429493E-926.4128575
GTACATG96900.024.39871
TACATGG96050.023.9707762
ACGCAGA103950.023.2107077
CGCAGAG105000.022.7976638
ACATGGG99650.022.4237613
GGACCGA12200.021.0224346
ATTCGCG6700.020.55948380-81
AATTCGC7450.019.76413578-79
CGATCGT7450.019.44759224-25
TCGCGGA6750.019.35164582-83
GCAGAGT121550.019.2637629
CAATTCG7650.018.93698378-79
Produced by FastQC (version 0.11.5)