FastQCFastQC Report
Thu 26 May 2016
SRR938536_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938536_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296251
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44750.13576029252626698No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33310.10105419763240117No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA42600.040.0831571
GTATCAA65150.038.7284131
ATCAACG77850.031.6233083
TCAACGC79950.030.7332294
TATCAAC81750.030.2890822
CAACGCA83250.029.5720655
AACGCAG85950.028.6918586
GTACATG95850.027.5182911
TACATGG99800.025.715762
ACATGGG101750.024.2887443
ACGCAGA102450.024.2060137
CGCAGAG105250.023.3814988
GGACCGA12400.020.6923266
GCAGAGT114350.020.606169
CATGGGG74850.020.2551294
AGGACCG14150.020.1525925
GAGTACT53800.019.99958612-13
ACCGAGT12450.019.4610188
GTATAGG7200.017.8862671
CAGAGTA111200.017.66481410-11
Produced by FastQC (version 0.11.5)