##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938536_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3296251 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.038258008871292 31.0 31.0 34.0 28.0 34.0 2 31.216934936083447 31.0 31.0 34.0 28.0 34.0 3 31.28633590099783 31.0 31.0 34.0 27.0 34.0 4 34.94256080620074 37.0 35.0 37.0 32.0 37.0 5 34.92357954536836 37.0 35.0 37.0 32.0 37.0 6 34.891235831251926 37.0 35.0 37.0 32.0 37.0 7 34.79550768433593 37.0 35.0 37.0 32.0 37.0 8 34.74858255636479 37.0 35.0 37.0 31.0 37.0 9 36.229475243238454 39.0 35.0 39.0 32.0 39.0 10-11 36.05179384094233 38.5 35.0 39.0 30.0 39.0 12-13 35.860872093781694 38.0 35.0 39.0 30.0 39.0 14-15 37.041145683383945 39.0 36.0 41.0 31.0 41.0 16-17 36.96165340564174 39.0 36.0 41.0 31.0 41.0 18-19 36.912873898255924 39.0 36.0 41.0 30.5 41.0 20-21 36.823071119280655 39.0 36.0 41.0 30.0 41.0 22-23 36.667524712165424 39.0 36.0 41.0 30.0 41.0 24-25 36.52451254470609 39.0 36.0 41.0 30.0 41.0 26-27 36.31852034326269 39.0 35.5 40.5 29.0 41.0 28-29 36.075331945291786 39.0 35.0 40.0 28.0 41.0 30-31 35.83110752184831 38.5 35.0 40.0 27.0 41.0 32-33 35.57131207544571 38.0 34.5 40.0 27.0 41.0 34-35 35.27366954154887 38.0 34.0 40.0 26.0 41.0 36-37 35.10260338184197 38.0 34.0 40.0 25.0 41.0 38-39 34.88557834339679 38.0 34.0 40.0 25.0 41.0 40-41 34.62396947319849 38.0 33.0 40.0 24.0 41.0 42-43 34.2307371313653 38.0 33.0 40.0 23.5 41.0 44-45 33.806539914587816 38.0 33.0 40.0 22.5 41.0 46-47 33.36498813348862 37.5 32.0 40.0 20.0 41.0 48-49 32.92425106583207 37.0 31.0 40.0 17.5 41.0 50-51 32.20889686495355 36.5 30.5 39.0 15.0 40.5 52-53 32.210385222484575 36.5 31.0 39.0 14.5 40.0 54-55 32.18117370309482 36.5 31.0 39.0 11.0 40.0 56-57 31.748981949493533 36.0 30.0 39.0 8.0 40.0 58-59 31.27880006710654 36.0 29.5 39.0 6.5 40.0 60-61 30.720388556575333 35.0 28.5 39.0 2.0 40.0 62-63 29.977896100752034 34.5 28.0 38.0 2.0 40.0 64-65 29.29212975589541 34.0 26.5 38.0 2.0 40.0 66-67 28.57984904668971 34.0 26.0 37.5 2.0 39.5 68-69 27.725893750202882 33.5 24.5 36.5 2.0 39.0 70-71 26.885120702276616 32.5 22.5 36.0 2.0 39.0 72-73 25.970112712897166 31.5 20.0 35.5 2.0 38.5 74-75 25.01319999599545 31.0 15.5 35.0 2.0 37.5 76-77 24.05290085615446 30.5 7.5 34.5 2.0 37.0 78-79 23.061685077987086 29.5 2.0 34.0 2.0 36.0 80-81 22.079294325583824 29.0 2.0 34.0 2.0 35.5 82-83 21.072657088310326 28.0 2.0 34.0 2.0 35.0 84-85 20.088516469164517 26.0 2.0 33.0 2.0 35.0 86-87 19.084115105312065 25.5 2.0 32.5 2.0 35.0 88-89 18.00644777961387 23.0 2.0 32.0 2.0 34.0 90-91 16.860120482329776 19.0 2.0 31.5 2.0 34.0 92-93 15.69356641833404 8.0 2.0 31.0 2.0 34.0 94-95 14.44105121242284 2.0 2.0 30.5 2.0 34.0 96-97 13.024391346411424 2.0 2.0 29.5 2.0 34.0 98-99 11.335641005493816 2.0 2.0 28.0 2.0 34.0 100-101 8.026465065918828 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5761736367674075 1101 2 0.48751963330624193 1101 3 0.3701473321526869 1101 4 0.38974961434589517 1101 5 0.40923773862243706 1101 6 0.4423122497205014 1101 7 0.3826710837186411 1101 8 0.4740811244006693 1101 9 0.47123270367374914 1101 10-11 0.5966065359127768 1101 12-13 0.5486870643821504 1101 14-15 0.575795623796175 1101 16-17 0.6537248133537474 1101 18-19 0.5617615757038692 1101 20-21 0.7592695600838653 1101 22-23 0.788272784708731 1101 24-25 0.8381465314777117 1101 26-27 0.9996391232553137 1101 28-29 0.9287523512625739 1101 30-31 0.9685833082973687 1101 32-33 0.8180026901200179 1101 34-35 0.9337809822850787 1101 36-37 1.109542799262357 1101 38-39 1.1481601374335995 1101 40-41 1.1464448843008483 1101 42-43 1.2780472140594341 1101 44-45 1.3776697201307897 1101 46-47 1.3949950698609683 1101 48-49 1.3360696237748755 1101 50-51 1.4869796034876543 1101 52-53 1.541628307446679 1101 54-55 1.560711925922348 1101 56-57 1.7803799604413761 1101 58-59 1.8192039090031997 1101 60-61 1.835799405660989 1101 62-63 1.7195654209095927 1101 64-65 1.7390199050928459 1101 66-67 1.938875735314383 1101 68-69 2.2955621201518888 1101 70-71 2.5469861457536496 1101 72-73 2.8383528465218753 1101 74-75 2.526197767317438 1101 76-77 2.7960057999524217 1101 78-79 3.011322716939034 1101 80-81 3.163946089240028 1101 82-83 3.2019563190365545 1101 84-85 3.3112590944106657 1101 86-87 3.4590758564975914 1101 88-89 3.724517637260643 1101 90-91 3.729930225225477 1101 92-93 3.8754442756099987 1101 94-95 3.7587243954938803 1101 96-97 3.7477640255196594 1101 98-99 3.3387380525138415 1101 100-101 2.5534277933531166 1102 1 0.5639442209423855 1102 2 0.5609947359789622 1102 3 0.5132206822649259 1102 4 0.4018479586155834 1102 5 0.3946692428355334 1102 6 0.4479204469973155 1102 7 0.5532451359665131 1102 8 0.6238111506767297 1102 9 0.6492427511832091 1102 10-11 0.7521093707444706 1102 12-13 0.7114824735658374 1102 14-15 0.8181574546365837 1102 16-17 0.8427582967566849 1102 18-19 0.7990307480295655 1102 20-21 0.7969873881917096 1102 22-23 0.6891853552099789 1102 24-25 0.7817337457047628 1102 26-27 0.8245278995447052 1102 28-29 0.8273725728810888 1102 30-31 0.7654331564283794 1102 32-33 0.7549070325833327 1102 34-35 0.7857532490034345 1102 36-37 1.0552824965796859 1102 38-39 1.1479780780141198 1102 40-41 1.035893090828452 1102 42-43 1.3029187667418753 1102 44-45 1.3090162450692802 1102 46-47 1.3095551737003248 1102 48-49 1.3728227053597593 1102 50-51 1.4611269320323075 1102 52-53 1.4146061708549595 1102 54-55 1.4661317768357023 1102 56-57 1.7015145985276483 1102 58-59 1.8933680027318047 1102 60-61 1.8100807059881099 1102 62-63 1.7706155931550178 1102 64-65 2.004622472186796 1102 66-67 2.2558467979706975 1102 68-69 2.3706833823906166 1102 70-71 2.2424128524297267 1102 72-73 2.3744377999751016 1102 74-75 2.0611127130111484 1102 76-77 2.4447085377106994 1102 78-79 2.7531161500389345 1102 80-81 2.8730203292542207 1102 82-83 3.082768584714575 1102 84-85 3.1809831836411107 1102 86-87 3.2747845251268117 1102 88-89 3.268075072703926 1102 90-91 3.075383623346763 1102 92-93 3.0438537760804873 1102 94-95 3.0707980504106356 1102 96-97 3.0768036349507515 1102 98-99 2.894540574364246 1102 100-101 2.1050412645526455 1103 1 0.47523351486177745 1103 2 0.41718033943326915 1103 3 0.3777514147601444 1103 4 0.4170476571508246 1103 5 0.3776657485849384 1103 6 0.4291603520096814 1103 7 0.3883967211346828 1103 8 0.3265052003921767 1103 9 0.4306873621362328 1103 10-11 0.5018222831447616 1103 12-13 0.4772450496609082 1103 14-15 0.594591015769204 1103 16-17 0.6441812344636872 1103 18-19 0.6930937837563391 1103 20-21 0.5488525009348564 1103 22-23 0.6832243925636234 1103 24-25 0.7066556462155731 1103 26-27 0.7425068538643416 1103 28-29 0.7207379793628661 1103 30-31 0.6546016720205756 1103 32-33 0.9204018728419996 1103 34-35 0.8985192719719066 1103 36-37 0.8905703823029683 1103 38-39 0.8851064221154346 1103 40-41 1.0432867581734584 1103 42-43 1.01063142840745 1103 44-45 1.024112096430244 1103 46-47 1.2771173726728975 1103 48-49 1.1161040719805584 1103 50-51 1.2997310394167343 1103 52-53 1.3880881507553724 1103 54-55 1.507537352621732 1103 56-57 1.650147784578639 1103 58-59 1.700216722964342 1103 60-61 1.7978552021862804 1103 62-63 2.0179592812002163 1103 64-65 2.021982024304627 1103 66-67 2.2598879818758633 1103 68-69 2.405138723185736 1103 70-71 2.3600001430954016 1103 72-73 2.6503663383958234 1103 74-75 2.472029549263894 1103 76-77 2.522619338108381 1103 78-79 2.8877082104951555 1103 80-81 2.9187581853849736 1103 82-83 2.930118431795556 1103 84-85 3.0919975075903565 1103 86-87 3.1218970306662968 1103 88-89 3.257640426997906 1103 90-91 3.296769071112312 1103 92-93 3.0560019274951014 1103 94-95 3.058870658518112 1103 96-97 3.1769850896202474 1103 98-99 2.8165430372496782 1103 100-101 2.1729466384389164 1104 1 0.5762676813765424 1104 2 0.41543869023531244 1104 3 0.47471698621475866 1104 4 0.4924640579893236 1104 5 0.4518588651543851 1104 6 0.44580926515222785 1104 7 0.39044881965819656 1104 8 0.3818890752554651 1104 9 0.5554879910097341 1104 10-11 0.5839268732377221 1104 12-13 0.5819476449033374 1104 14-15 0.6961563639281607 1104 16-17 0.6196991721687581 1104 18-19 0.674844917323604 1104 20-21 0.6900378762082795 1104 22-23 0.7788242718016818 1104 24-25 0.8411129580092265 1104 26-27 0.83065594928906 1104 28-29 0.8431048564135466 1104 30-31 0.8214453992353086 1104 32-33 0.8990698743458196 1104 34-35 0.9070630367823327 1104 36-37 1.0556909733529167 1104 38-39 0.9356925732273638 1104 40-41 0.883575533009406 1104 42-43 1.0042209346506112 1104 44-45 1.0451777920645924 1104 46-47 1.122979994574095 1104 48-49 1.1257151843575173 1104 50-51 1.1705385494422558 1104 52-53 1.314500980002542 1104 54-55 1.5814677265280395 1104 56-57 1.8103218355720792 1104 58-59 1.8390139169581516 1104 60-61 1.7878695569073848 1104 62-63 1.723671121566607 1104 64-65 1.597901125197808 1104 66-67 1.9753277091361632 1104 68-69 2.3095267392721404 1104 70-71 2.4067669130552964 1104 72-73 2.6229136463159826 1104 74-75 2.5944306042219694 1104 76-77 2.7444261247015262 1104 78-79 2.9573513931418915 1104 80-81 3.0224162183324808 1104 82-83 3.190869519792276 1104 84-85 3.1369443039811706 1104 86-87 3.2221764350592963 1104 88-89 3.2317335731882046 1104 90-91 3.0243184581885068 1104 92-93 3.0360384693744304 1104 94-95 3.1435749056927396 1104 96-97 3.1175767661605303 1104 98-99 2.77170375885923 1104 100-101 2.0106015782858 1105 1 0.5850628941172253 1105 2 0.40604809071128045 1105 3 0.36558913070003385 1105 4 0.35458191419641594 1105 5 0.30223715679395724 1105 6 0.3527835802834858 1105 7 0.46951749116421126 1105 8 0.3870423061090733 1105 9 0.5468270096914907 1105 10-11 0.555571630711583 1105 12-13 0.5402886005224588 1105 14-15 0.6311802947943974 1105 16-17 0.6595649288674323 1105 18-19 0.6221546294336875 1105 20-21 0.5077624228081419 1105 22-23 0.6118412307794685 1105 24-25 0.6628569805533147 1105 26-27 0.5946468174795712 1105 28-29 0.6483615354025787 1105 30-31 0.7306818362615886 1105 32-33 0.7101872567550274 1105 34-35 0.8126707283955668 1105 36-37 0.7737036762952059 1105 38-39 1.0093691209784552 1105 40-41 1.0012371384783165 1105 42-43 1.0486332080523582 1105 44-45 1.156671412762904 1105 46-47 1.2488664565121113 1105 48-49 1.278500113036678 1105 50-51 1.203677037918382 1105 52-53 1.3137980892923764 1105 54-55 1.588212049438269 1105 56-57 1.7264920637553125 1105 58-59 1.6450357235400048 1105 60-61 1.87719624123174 1105 62-63 1.898862897711357 1105 64-65 2.0923586486738834 1105 66-67 2.195913336565397 1105 68-69 2.370948857555984 1105 70-71 2.3405345667782775 1105 72-73 2.441028117379691 1105 74-75 2.6290782204348133 1105 76-77 2.8690226667914764 1105 78-79 3.2204704783046907 1105 80-81 3.3714983961329423 1105 82-83 3.502072472612049 1105 84-85 3.638212672134351 1105 86-87 3.5804395500292756 1105 88-89 3.6908601899047184 1105 90-91 3.540286312562852 1105 92-93 3.5156651600794415 1105 94-95 3.3651596323629445 1105 96-97 3.2592580325449116 1105 98-99 2.992032887179718 1105 100-101 2.201325932447687 1106 1 0.23104775194768123 1106 2 0.16158823878756579 1106 3 0.12433602312623293 1106 4 0.19510580566289804 1106 5 0.1825268694440041 1106 6 -0.2630777691301276 1106 7 0.23851840487976972 1106 8 -0.14010265685094936 1106 9 0.10513816224852945 1106 10-11 0.20147833136205406 1106 12-13 0.34925033110391723 1106 14-15 0.4874791404182801 1106 16-17 -0.14177490850374141 1106 18-19 0.2880390830966064 1106 20-21 0.3765669608590585 1106 22-23 0.2864803758595187 1106 24-25 0.38333754893875494 1106 26-27 0.38736306175317026 1106 28-29 0.4642217139221003 1106 30-31 0.2861475907460189 1106 32-33 0.4567453508740016 1106 34-35 0.23107914879672364 1106 36-37 0.4230969649812337 1106 38-39 0.4561407778144826 1106 40-41 0.6507201260145621 1106 42-43 0.29398448673142497 1106 44-45 0.7205836626149704 1106 46-47 0.9720463539334219 1106 48-49 0.7518332094033511 1106 50-51 0.5232929428417776 1106 52-53 0.7243093309210096 1106 54-55 0.5107528477789387 1106 56-57 1.084195940460166 1106 58-59 1.065528126319201 1106 60-61 1.4475144526073898 1106 62-63 1.3678885256555837 1106 64-65 1.1696698576847524 1106 66-67 1.441448069256552 1106 68-69 1.557065440970689 1106 70-71 1.238563450226227 1106 72-73 1.5858621535979545 1106 74-75 1.7990393927506965 1106 76-77 1.9754965401718287 1106 78-79 2.4297014727073964 1106 80-81 2.068527670575879 1106 82-83 2.412208944499092 1106 84-85 2.5515249851310635 1106 86-87 2.287871465674719 1106 88-89 2.1460724876379906 1106 90-91 2.2752608769798 1106 92-93 2.302145640647824 1106 94-95 2.350694072330578 1106 96-97 2.3504853177224376 1106 98-99 2.2362960288976304 1106 100-101 1.7601518602244317 1107 1 0.370930431864096 1107 2 0.3065772504157138 1107 3 0.32862751201945883 1107 4 0.3255841841198759 1107 5 0.2853832645102443 1107 6 0.29079981749936934 1107 7 0.32460774927486824 1107 8 0.3576397624187919 1107 9 0.4250717750340982 1107 10-11 0.4763611982261935 1107 12-13 0.4353858723591202 1107 14-15 0.5259521098544084 1107 16-17 0.4848117583844953 1107 18-19 0.639349711282506 1107 20-21 0.6183565075731394 1107 22-23 0.6422490425898886 1107 24-25 0.6238534415725994 1107 26-27 0.6062423309121883 1107 28-29 0.6248866662088943 1107 30-31 0.7664292586520745 1107 32-33 0.8946114049060512 1107 34-35 0.8400088938415706 1107 36-37 0.8385591620739916 1107 38-39 1.0172081662378574 1107 40-41 0.9572829882257778 1107 42-43 1.1554309220537462 1107 44-45 1.075732452232316 1107 46-47 1.1696400874456714 1107 48-49 0.9669635119678617 1107 50-51 1.2385020542584684 1107 52-53 1.4719726505309936 1107 54-55 1.6900486364698537 1107 56-57 1.7686087791990595 1107 58-59 1.7621461173374087 1107 60-61 1.8845421084075475 1107 62-63 2.0783419777491936 1107 64-65 2.112295393082263 1107 66-67 2.2038642232017693 1107 68-69 2.237047950972393 1107 70-71 2.5495783686547036 1107 72-73 2.7016402092585245 1107 74-75 2.695866515957558 1107 76-77 2.7825763336153635 1107 78-79 3.0108967749187805 1107 80-81 3.0181973962612503 1107 82-83 3.1292563752141866 1107 84-85 3.0695320808358346 1107 86-87 2.989972633474842 1107 88-89 3.2248676718645903 1107 90-91 2.968311780768193 1107 92-93 2.913685632920652 1107 94-95 2.8785671472883063 1107 96-97 2.6489743646629353 1107 98-99 2.551186622934413 1107 100-101 1.6919284036856235 1108 1 0.26496244427733373 1108 2 0.13642084491242557 1108 3 0.07101057354828555 1108 4 0.1353941064417583 1108 5 0.05720451899748724 1108 6 0.08951938456232256 1108 7 0.1345898480147838 1108 8 0.1792632445477409 1108 9 0.261334386201554 1108 10-11 0.1421426057167423 1108 12-13 0.16869024465445648 1108 14-15 0.23939387352597663 1108 16-17 0.3824717171130203 1108 18-19 0.2751628036854399 1108 20-21 0.2774473588527826 1108 22-23 0.3595266997187565 1108 24-25 0.3777440244923227 1108 26-27 0.35182377210671234 1108 28-29 0.3507884064148712 1108 30-31 0.4294657002758484 1108 32-33 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0.24209715424460931 2316 96-97 0.2066359177545305 2316 98-99 0.14171670192242303 2316 100-101 0.5146298735045045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10675.0 3 4359.0 4 4970.0 5 5601.0 6 6808.0 7 8563.0 8 10874.0 9 13901.0 10 17448.0 11 21829.0 12 25736.0 13 30291.0 14 34477.0 15 38484.0 16 43350.0 17 49040.0 18 51508.0 19 54975.0 20 60779.0 21 67673.0 22 75012.0 23 82988.0 24 91623.0 25 101234.0 26 111541.0 27 122998.0 28 134810.0 29 148334.0 30 164946.0 31 183523.0 32 206198.0 33 231098.0 34 260349.0 35 285915.0 36 283721.0 37 201429.0 38 48592.0 39 599.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.63341401644767 17.992902971956628 13.893230292834632 24.48045271876108 2 15.775729931049803 19.198477093124495 39.780419066439634 25.24537390938607 3 19.596536889027007 22.72542147789183 28.623471657068496 29.05456997601266 4 13.248911006002215 15.586788086867 35.75251063499155 35.411790272139235 5 14.805811317066123 36.298510742995326 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26.655593121395647 23.55409526485333 52-53 23.523133940043962 26.370886385116123 26.557572024763985 23.548407650075927 54-55 23.37332081695464 26.23342358556628 26.6773322880094 23.715923309469687 56-57 23.327146414557944 26.43379377459945 26.85413005272408 23.38492975811853 58-59 23.500964623981716 26.126671405596248 26.696647525881325 23.675716444540722 60-61 23.510927608471697 26.104081433585968 26.94226751232143 23.44272344562091 62-63 23.377087559628308 26.203413157337412 26.825516697746306 23.59398258528797 64-65 23.26071526038395 26.079490265211785 26.827581467593102 23.83221300681116 66-67 23.271077557718478 26.163611521654413 26.715232047265463 23.850078873361646 68-69 23.09533337601103 26.025667903409655 26.914481482683826 23.96451723789549 70-71 23.159205972681647 26.00051398656353 26.839580363650047 24.00069967710478 72-73 23.324048513273308 25.953360277060934 26.628551420917283 24.09403978874847 74-75 23.169763381796184 26.165235274092513 26.607381143241 24.057620200870307 76-77 23.32211234849888 26.006805405186473 26.54064030759182 24.130441938722836 78-79 23.372368238611212 25.98788994577873 26.628921227000863 24.010820588609192 80-81 23.223007721832 26.188069845411654 26.561615364776912 24.02730706797943 82-83 23.417488483439897 26.10242285820048 26.3722361583198 24.107852500039822 84-85 23.46150876321635 25.7383385045561 26.406716455835618 24.393436276391935 86-87 23.39257922121263 25.607071886898353 26.636272621088242 24.364076270800783 88-89 23.436561990673024 25.71029091004532 26.52309009638137 24.330057002900286 90-91 23.580715860960836 25.73454987668426 26.407290156513508 24.277444105841393 92-93 23.540889530024774 25.340572255749706 26.51215386209446 24.60638435213106 94-95 23.41733223407622 25.4472913396483 26.45388839950976 24.681488026765717 96-97 23.471855005194012 25.38169204200179 26.316280555883008 24.83017239692119 98-99 23.568277761418894 25.239751209432303 26.062812958481757 25.129158070667046 100-101 24.51003099017442 24.36603792784248 26.01745503918765 25.10647604279545 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 187.0 1 144.5 2 132.0 3 162.5 4 143.0 5 161.0 6 282.5 7 572.5 8 910.5 9 1092.0 10 1036.0 11 941.0 12 1010.5 13 1153.0 14 1304.0 15 1456.0 16 1615.0 17 1752.0 18 1903.0 19 2098.0 20 2410.0 21 2901.5 22 3501.0 23 4195.5 24 5254.0 25 6809.5 26 8719.0 27 11383.0 28 14567.5 29 18383.0 30 23194.5 31 28310.0 32 33927.5 33 39663.0 34 45456.0 35 51803.5 36 58826.0 37 66274.0 38 73476.0 39 79024.0 40 85865.0 41 96273.0 42 106724.5 43 115753.0 44 123431.5 45 129870.5 46 134491.0 47 138198.0 48 141508.5 49 141208.5 50 138291.5 51 134584.0 52 130540.0 53 127098.0 54 124083.5 55 117510.0 56 107579.5 57 98927.5 58 90103.5 59 79788.5 60 67686.0 61 56080.5 62 45884.0 63 36960.0 64 30524.0 65 24862.5 66 19170.5 67 15068.5 68 11414.0 69 7996.0 70 5958.0 71 4387.0 72 3308.5 73 2588.0 74 1992.0 75 1604.5 76 1135.0 77 680.0 78 430.5 79 259.0 80 139.5 81 79.0 82 53.0 83 32.0 84 20.5 85 13.0 86 11.0 87 11.5 88 7.5 89 4.5 90 4.5 91 4.0 92 3.5 93 2.5 94 2.0 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3829502061584509 2 0.029548720652644474 3 0.03576790723764665 4 0.04450510595218629 5 0.0624345658143145 6 0.05324230466672592 7 0.03719376952786666 8 0.03886233178237943 9 0.03564655725550026 10-11 0.03726961326670815 12-13 0.030049289328998308 14-15 0.027334083478472968 16-17 0.022404240453776123 18-19 0.0270762147664119 20-21 0.033553270063475145 22-23 0.032673482692913855 24-25 0.04124382518200222 26-27 0.03531284480459771 28-29 0.03115660791608406 30-31 0.03399316374875578 32-33 0.02866893328208319 34-35 0.029852095608010432 36-37 0.03191504530449896 38-39 0.015456953975895646 40-41 0.013120966819577758 42-43 0.013970416694602443 44-45 0.012089491971333494 46-47 0.02009859079299483 48-49 0.017504734924615874 50-51 0.012377698178931156 52-53 0.010193398500296245 54-55 0.009192261147588579 56-57 0.009662492328405816 58-59 0.0044899493394162035 60-61 0.008024267569429634 62-63 0.01265073563876052 64-65 0.007963592578356443 66-67 0.004383768105038118 68-69 0.006916948982343881 70-71 0.013500185513785207 72-73 0.01120970460077221 74-75 0.011922635745882216 76-77 0.005900642881867916 78-79 0.009465298607417943 80-81 0.012165335710174982 82-83 0.013758054225846271 84-85 0.008691692471234747 86-87 0.003064087049196193 88-89 0.01642775383306672 90-91 0.02574136496280168 92-93 0.011058017123089231 94-95 0.02099354691132441 96-97 0.01909745344028716 98-99 0.011725442024894343 100-101 0.12373147554600666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3296251.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.684368138732104 #Duplication Level Percentage of deduplicated Percentage of total 1 75.26333168534724 38.146744104876404 2 13.005308114806741 13.183316484970097 3 4.657413053970457 7.081741134047256 4 2.2008269676336085 4.46190096948765 5 1.2151068738223523 3.0793462060358006 6 0.7720160839093575 2.3477488443530503 7 0.53661121206093 1.903846015362719 8 0.3413553945945933 1.3841105988619624 9 0.2780917911759613 1.2685416048289653 >10 1.530434809345127 14.12399942482725 >50 0.1235492559342735 4.319747068635804 >100 0.07191100524456731 6.739618116045136 >500 0.003138434509081148 1.0526122891690288 >1k 9.053176458346836E-4 0.9067271384988498 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4475 0.13576029252626698 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3331 0.10105419763240117 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 6.067499107319194E-5 0.0 20-21 0.0 0.0 0.0 1.3651872991468186E-4 0.0 22-23 0.0 0.0 0.0 1.8202497321957581E-4 0.0 24-25 0.0 0.0 0.0 3.6404994643915163E-4 0.0 26-27 0.0 0.0 0.0 6.067499107319193E-4 1.5168747768297985E-5 28-29 0.0 0.0 0.0 0.0023663246518544857 3.033749553659597E-5 30-31 0.0 0.0 0.0 0.008706861219003043 3.033749553659597E-5 32-33 0.0 0.0 0.0 0.023496390293093576 4.5506243304893954E-5 34-35 0.0 0.0 0.0 0.049798998923322284 6.067499107319194E-5 36-37 0.0 0.0 0.0 0.09181643024150771 6.067499107319194E-5 38-39 0.0 0.0 0.0 0.15695103315857922 6.067499107319194E-5 40-41 0.0 0.0 0.0 0.22977619119417786 6.067499107319194E-5 42-43 0.0 0.0 0.0 0.3042395739887527 6.067499107319194E-5 44-45 0.0 0.0 0.0 0.3978610852146878 6.067499107319194E-5 46-47 0.0 0.0 0.0 0.5133407619747404 6.067499107319194E-5 48-49 0.0 0.0 0.0 0.634478381652368 6.067499107319194E-5 50-51 0.0 0.0 0.0 0.7603183131381681 6.067499107319194E-5 52-53 0.0 0.0 0.0 0.8865374633181757 6.067499107319194E-5 54-55 0.0 0.0 0.0 1.0294725735388477 6.067499107319194E-5 56-57 0.0 3.033749553659597E-5 0.0 1.1961012677736012 6.067499107319194E-5 58-59 0.0 3.033749553659597E-5 0.0 1.3768824036761762 6.067499107319194E-5 60-61 0.0 3.033749553659597E-5 0.0 1.5683878442509385 6.067499107319194E-5 62-63 0.0 3.033749553659597E-5 0.0 1.7673714774754714 6.067499107319194E-5 64-65 0.0 6.067499107319194E-5 0.0 1.9764119904703858 6.067499107319194E-5 66-67 0.0 6.067499107319194E-5 0.0 2.2111786996803335 7.584373884148993E-5 68-69 0.0 6.067499107319194E-5 0.0 2.4721570050339006 1.2134998214638388E-4 70-71 0.0 6.067499107319194E-5 0.0 2.7514136514482663 1.5168747768297985E-4 72-73 0.0 6.067499107319194E-5 0.0 3.0357821658605486 1.5168747768297985E-4 74-75 0.0 6.067499107319194E-5 0.0 3.3260209856591625 2.1236246875617177E-4 76-77 0.0 6.067499107319194E-5 0.0 3.6397410270031014 2.1236246875617177E-4 78-79 0.0 6.067499107319194E-5 0.0 3.984071601343466 2.1236246875617177E-4 80-81 0.0 6.067499107319194E-5 0.0 4.352338459662204 2.4269996429276776E-4 82-83 0.0 6.067499107319194E-5 0.0 4.724245817445334 2.5786871206106573E-4 84-85 0.0 6.067499107319194E-5 0.0 5.097943087465124 2.730374598293637E-4 86-87 0.0 7.584373884148993E-5 0.0 5.487264167686259 2.730374598293637E-4 88-89 0.0 9.101248660978791E-5 0.0 5.901340644265257 2.730374598293637E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4260 0.0 40.083157 1 GTATCAA 6515 0.0 38.728413 1 ATCAACG 7785 0.0 31.623308 3 TCAACGC 7995 0.0 30.733229 4 TATCAAC 8175 0.0 30.289082 2 CAACGCA 8325 0.0 29.572065 5 AACGCAG 8595 0.0 28.691858 6 GTACATG 9585 0.0 27.518291 1 TACATGG 9980 0.0 25.71576 2 ACATGGG 10175 0.0 24.288744 3 ACGCAGA 10245 0.0 24.206013 7 CGCAGAG 10525 0.0 23.381498 8 GGACCGA 1240 0.0 20.692326 6 GCAGAGT 11435 0.0 20.60616 9 CATGGGG 7485 0.0 20.255129 4 AGGACCG 1415 0.0 20.152592 5 GAGTACT 5380 0.0 19.999586 12-13 ACCGAGT 1245 0.0 19.461018 8 GTATAGG 720 0.0 17.886267 1 CAGAGTA 11120 0.0 17.664814 10-11 >>END_MODULE