FastQCFastQC Report
Thu 26 May 2016
SRR938536_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938536_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3296251
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45280.13736817978970656No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38350.11634429538284555No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47550.038.175211
GTATCAA66000.034.7033731
ATCAACG81900.027.3734173
TCAACGC83050.026.8795874
TATCAAC85250.026.4709152
CAACGCA85250.026.2416325
GTACATG91450.025.7730961
AACGCAG88950.025.3102686
TACATGG91550.025.0644722
ACATGGG97250.022.6620543
GGACCGA10200.021.885786
ACGCAGA103000.021.4427287
CGCAGAG105800.020.8303558
AGGACCG11700.019.8918235
CATGGGG69250.019.821774
ACCGAGT11500.018.998728
GAGTACT57400.018.99044412-13
ATTCGCG6300.018.85022580-81
AATTCGC6450.018.77894478-79
TCGCGGA6150.018.53758682-83
Produced by FastQC (version 0.11.5)