##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938536_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3296251 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81336372745886 31.0 31.0 34.0 30.0 34.0 2 32.07549121714335 33.0 31.0 34.0 30.0 34.0 3 32.14135816720268 33.0 31.0 34.0 30.0 34.0 4 35.78626384944593 37.0 35.0 37.0 35.0 37.0 5 35.672099606492345 37.0 35.0 37.0 33.0 37.0 6 35.69924468737363 37.0 35.0 37.0 33.0 37.0 7 35.620961207141086 37.0 35.0 37.0 33.0 37.0 8 35.592469141457975 37.0 35.0 37.0 33.0 37.0 9 37.142669657134725 39.0 37.0 39.0 33.0 39.0 10-11 37.069666418000324 39.0 37.0 39.0 33.0 39.0 12-13 36.94412728278277 39.0 37.0 39.0 33.0 39.0 14-15 37.98563792623802 40.0 37.0 41.0 33.0 41.0 16-17 38.0758341825304 40.0 37.0 41.0 33.0 41.0 18-19 38.18235534854597 40.0 37.5 41.0 33.0 41.0 20-21 38.121638188353984 40.0 37.0 41.0 33.0 41.0 22-23 37.991133260179524 40.0 37.0 41.0 33.0 41.0 24-25 37.87310295848222 40.0 37.0 41.0 32.0 41.0 26-27 37.66401375380698 40.0 37.0 41.0 32.0 41.0 28-29 37.524802267788466 39.5 36.5 41.0 32.0 41.0 30-31 37.299184740482445 39.0 36.0 41.0 31.0 41.0 32-33 37.25326894098781 39.0 36.0 41.0 31.0 41.0 34-35 37.1372010201893 39.0 36.0 41.0 31.0 41.0 36-37 37.03668895360214 39.0 36.0 41.0 31.0 41.0 38-39 36.84747278044057 39.0 35.5 40.5 30.0 41.0 40-41 36.64308892132304 39.0 35.0 40.0 30.0 41.0 42-43 36.408006095409604 39.0 35.0 40.0 30.0 41.0 44-45 36.15264356385482 38.0 35.0 40.0 29.5 41.0 46-47 35.79376327834258 38.0 35.0 40.0 28.5 41.0 48-49 35.43583983743956 38.0 34.0 40.0 27.5 41.0 50-51 35.05726733188705 38.0 34.0 40.0 26.5 41.0 52-53 34.45094684840444 38.0 33.0 40.0 25.0 41.0 54-55 34.01168008746907 37.0 33.0 40.0 24.0 41.0 56-57 33.60716720298302 37.0 32.0 39.5 23.0 40.5 58-59 33.051806734377934 36.0 31.0 39.0 22.0 40.0 60-61 32.53249115434474 36.0 31.0 39.0 20.5 40.0 62-63 31.993384302348336 35.0 30.5 38.5 18.5 40.0 64-65 31.304046930892095 35.0 29.5 38.0 14.5 40.0 66-67 30.6251317026525 34.0 28.5 38.0 9.5 40.0 68-69 29.873887182741846 34.0 28.0 37.0 7.5 39.0 70-71 29.072228874560828 33.5 26.5 36.0 4.5 39.0 72-73 28.455408432185536 33.0 26.0 36.0 2.0 38.5 74-75 27.710169371203833 32.0 25.5 35.0 2.0 38.0 76-77 26.554646475647637 30.5 24.5 34.0 2.0 36.0 78-79 27.042558197176128 32.0 25.0 35.0 2.0 36.0 80-81 26.644864878311754 32.0 24.5 35.0 2.0 36.0 82-83 25.939109916083453 31.5 22.5 34.0 2.0 36.0 84-85 25.137898479211685 31.0 20.0 34.0 2.0 35.0 86-87 24.195219963528263 30.5 15.5 34.0 2.0 35.0 88-89 23.207489811910563 30.0 7.0 34.0 2.0 35.0 90-91 22.318711166109622 29.0 2.0 34.0 2.0 35.0 92-93 21.187870856922 29.0 2.0 33.5 2.0 35.0 94-95 19.98133364237129 26.5 2.0 33.0 2.0 34.0 96-97 18.57535758047552 25.0 2.0 32.5 2.0 34.0 98-99 16.785963659927596 12.5 2.0 32.0 2.0 34.0 100-101 12.573217573540365 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2986769299595231 1101 2 0.2985316011695005 1101 3 0.3054433660102873 1101 4 0.20897459582980815 1101 5 0.12248827074419211 1101 6 0.24126380527211921 1101 7 0.2655881941793439 1101 8 0.3304438177470388 1101 9 0.36920440344571404 1101 10-11 0.3029411441335341 1101 12-13 0.3149492826983291 1101 14-15 0.45448299560013794 1101 16-17 0.44850430958568666 1101 18-19 0.4598205311603252 1101 20-21 0.4570010314318651 1101 22-23 0.3760136497750466 1101 24-25 0.3748016128527638 1101 26-27 0.47641079272888476 1101 28-29 0.6310190888705165 1101 30-31 0.665168262310992 1101 32-33 0.5541894415426967 1101 34-35 0.3839627848933773 1101 36-37 0.4813059043864669 1101 38-39 0.5617631971451758 1101 40-41 0.6298697389923049 1101 42-43 0.6426777062390201 1101 44-45 0.7951133756814599 1101 46-47 0.8583776516683628 1101 48-49 1.0389159495356637 1101 50-51 1.2112234974860172 1101 52-53 1.2573778230834662 1101 54-55 1.2648316172828586 1101 56-57 1.3447745544228482 1101 58-59 1.4119664337151363 1101 60-61 1.3835764238835964 1101 62-63 1.463059863665066 1101 64-65 1.5685197278203198 1101 66-67 1.7115200587270358 1101 68-69 1.7140591307794217 1101 70-71 1.9635327211417923 1101 72-73 1.9975440393112258 1101 74-75 2.043049429865018 1101 76-77 1.9545397778026299 1101 78-79 2.067657997163547 1101 80-81 2.237362136506036 1101 82-83 2.2913410595758847 1101 84-85 2.507617229446261 1101 86-87 2.49762060873865 1101 88-89 2.332729588732324 1101 90-91 2.39228692284653 1101 92-93 2.8780378975360428 1101 94-95 2.897017616476848 1101 96-97 2.960379656660411 1101 98-99 3.0527916707623852 1101 100-101 2.5462458874039715 1102 1 0.1909674526347196 1102 2 0.19440680458301074 1102 3 0.1982194428412356 1102 4 0.17869585032212143 1102 5 0.1709368456942002 1102 6 0.2717047763879137 1102 7 0.15846287873286258 1102 8 0.22626382328927974 1102 9 0.2870756778934549 1102 10-11 0.29196172620350325 1102 12-13 0.3309154540748054 1102 14-15 0.36425009987677726 1102 16-17 0.35586366400636393 1102 18-19 0.37869640686520256 1102 20-21 0.22546743248762624 1102 22-23 0.30512849671134745 1102 24-25 0.3058685933177614 1102 26-27 0.399633360462758 1102 28-29 0.3029587270980514 1102 30-31 0.25810524561448034 1102 32-33 0.30260465452509067 1102 34-35 0.3528795538008467 1102 36-37 0.2682675684357534 1102 38-39 0.3414365904053085 1102 40-41 0.3925957953251995 1102 42-43 0.3053151465995967 1102 44-45 0.29762894849783805 1102 46-47 0.14679186173349734 1102 48-49 0.35547485249387734 1102 50-51 0.3494245595679075 1102 52-53 0.48211179273287286 1102 54-55 0.3957075476242622 1102 56-57 0.5669420569474468 1102 58-59 0.7737369774740515 1102 60-61 0.6271646765790777 1102 62-63 0.9199897131582446 1102 64-65 0.7774687902891522 1102 66-67 0.8880141294465496 1102 68-69 1.1873199721558976 1102 70-71 1.475960334746965 1102 72-73 1.4564321970102334 1102 74-75 1.3273272611462694 1102 76-77 1.3083966776020262 1102 78-79 1.5226833329864604 1102 80-81 1.775312544118929 1102 82-83 1.8638200277835963 1102 84-85 2.0676304600953337 1102 86-87 1.9955820297301976 1102 88-89 1.9928936499035395 1102 90-91 2.1207516137038347 1102 92-93 2.2268453147827394 1102 94-95 2.0799275509522985 1102 96-97 2.227293734531319 1102 98-99 2.5279710123883703 1102 100-101 2.194110412742507 1103 1 0.24233599767366698 1103 2 0.20037396270915053 1103 3 0.19333066263644838 1103 4 0.15345018132692445 1103 5 0.15794055131998874 1103 6 0.12983976933235652 1103 7 0.11932238857566091 1103 8 0.13535299300752257 1103 9 0.19040323363820022 1103 10-11 0.27879050063555866 1103 12-13 0.2944697242910266 1103 14-15 0.401210054482128 1103 16-17 0.332948315397644 1103 18-19 0.3211348420182034 1103 20-21 0.3376336783475864 1103 22-23 0.33808826691007 1103 24-25 0.3155690746701012 1103 26-27 0.45108900665859153 1103 28-29 0.49575768450813484 1103 30-31 0.3249715099879964 1103 32-33 0.3616433857491401 1103 34-35 0.4640984379147568 1103 36-37 0.4811728621452289 1103 38-39 0.44109680478348423 1103 40-41 0.5055599825365604 1103 42-43 0.4693709955698324 1103 44-45 0.6243706254174199 1103 46-47 0.7536049397353182 1103 48-49 0.6449987335173404 1103 50-51 0.729637053591091 1103 52-53 0.8097960025686675 1103 54-55 0.8938997430690492 1103 56-57 1.0120323396654172 1103 58-59 1.0265201145442973 1103 60-61 0.9624252293033351 1103 62-63 1.0945231206429682 1103 64-65 1.2953401072586495 1103 66-67 1.1045132569772242 1103 68-69 1.0991586593555986 1103 70-71 1.4025130130893189 1103 72-73 1.365775016959386 1103 74-75 1.2131199691394592 1103 76-77 1.224839335156723 1103 78-79 1.257981459473374 1103 80-81 1.5109364799541645 1103 82-83 1.5662894002470935 1103 84-85 1.7866813964145507 1103 86-87 1.6147136348405198 1103 88-89 1.714069909116585 1103 90-91 2.168117514366241 1103 92-93 2.1674109839528626 1103 94-95 1.9342435772736124 1103 96-97 1.843250611805427 1103 98-99 1.921933918228742 1103 100-101 1.6819534236582143 1104 1 0.19804142968241578 1104 2 0.23464158711673377 1104 3 0.20749028485664667 1104 4 0.16641927310600124 1104 5 0.051667196416602224 1104 6 0.040742236977273194 1104 7 0.08693597315458845 1104 8 0.044441183401254136 1104 9 0.13302137527144708 1104 10-11 0.2187484618094615 1104 12-13 0.12888417129247642 1104 14-15 0.3457984100690794 1104 16-17 0.2750929506054476 1104 18-19 0.28661444350380805 1104 20-21 0.31461200955429547 1104 22-23 0.3075260972965026 1104 24-25 0.23135328675541444 1104 26-27 0.31889740530969846 1104 28-29 0.2795140643841876 1104 30-31 0.41677835354431636 1104 32-33 0.44953899502730366 1104 34-35 0.3587607032264657 1104 36-37 0.34170563875842674 1104 38-39 0.34864627730445363 1104 40-41 0.4627250960072118 1104 42-43 0.40914881069014086 1104 44-45 0.49627538867844834 1104 46-47 0.56685471757892 1104 48-49 0.6915803731454844 1104 50-51 0.5685900418794603 1104 52-53 0.4906384310229157 1104 54-55 0.6049070229633244 1104 56-57 0.7030135019421166 1104 58-59 0.7420965534342017 1104 60-61 0.7951571264050514 1104 62-63 0.8981895556593713 1104 64-65 0.9492510567209145 1104 66-67 0.856474926454073 1104 68-69 1.0634626832345653 1104 70-71 1.1062254458727665 1104 72-73 1.086634078661902 1104 74-75 1.2139644482681469 1104 76-77 1.2659781430313117 1104 78-79 1.4091133178401272 1104 80-81 1.5589934659184372 1104 82-83 1.5842071682018855 1104 84-85 1.8436979783959586 1104 86-87 1.951512702922912 1104 88-89 2.048727161668534 1104 90-91 2.243425685164638 1104 92-93 2.549394669698401 1104 94-95 2.3316827975616157 1104 96-97 2.5198742048149043 1104 98-99 2.4487892089688046 1104 100-101 2.3400547269757492 1105 1 0.14877535822718002 1105 2 0.16794570970141365 1105 3 0.14635194469691015 1105 4 0.11977869021241361 1105 5 0.031878582906706754 1105 6 0.09342289448384378 1105 7 0.15140574715706379 1105 8 0.13633362245577274 1105 9 0.1387313865258406 1105 10-11 0.1836266658846668 1105 12-13 0.1944670818505685 1105 14-15 0.3844355928858292 1105 16-17 0.22048583460913562 1105 18-19 0.21476512311189566 1105 20-21 0.22770488480154683 1105 22-23 0.3312880712145443 1105 24-25 0.30470520149622615 1105 26-27 0.24443587525475152 1105 28-29 0.255704277946208 1105 30-31 0.29865207147302186 1105 32-33 0.429424221221673 1105 34-35 0.41099407821509004 1105 36-37 0.36199354734123546 1105 38-39 0.43667820537676505 1105 40-41 0.4455066069608975 1105 42-43 0.5041237784813859 1105 44-45 0.578918799086523 1105 46-47 0.6117586072016579 1105 48-49 0.5844431221099811 1105 50-51 0.5624915485974853 1105 52-53 0.6764914233484731 1105 54-55 0.5994298803175795 1105 56-57 0.7875929587986761 1105 58-59 0.8812574594063634 1105 60-61 0.9001303412119768 1105 62-63 0.7374680456424159 1105 64-65 1.0240344197627813 1105 66-67 1.099718088287954 1105 68-69 1.1982016311181525 1105 70-71 1.253469085916695 1105 72-73 1.4139470506779581 1105 74-75 1.3551053525704795 1105 76-77 1.4416420200827957 1105 78-79 1.6089139052680572 1105 80-81 1.6857412320088976 1105 82-83 1.9788528532471155 1105 84-85 2.0429507370129016 1105 86-87 2.26093776818891 1105 88-89 2.4108340299052067 1105 90-91 2.536008058457643 1105 92-93 2.5638651903737717 1105 94-95 2.4917159685049093 1105 96-97 2.642686325963261 1105 98-99 2.539146895830484 1105 100-101 2.30667144857944 1106 1 0.03237109031825014 1106 2 0.035438971833237076 1106 3 0.05443090477569967 1106 4 0.0861874953359063 1106 5 0.10614889635087366 1106 6 0.002711137243643691 1106 7 0.050005817828889576 1106 8 -0.002037956599437507 1106 9 -0.08777773912991194 1106 10-11 0.0014907041729017578 1106 12-13 0.057747508892369126 1106 14-15 0.1452899692070062 1106 16-17 0.014249945450984569 1106 18-19 0.16636316353701375 1106 20-21 0.1351826325867762 1106 22-23 0.20504231309199383 1106 24-25 -0.001893288468586718 1106 26-27 -0.15479936363900038 1106 28-29 0.18346613481695329 1106 30-31 0.26504858220865657 1106 32-33 0.09430367140327434 1106 34-35 0.039033604253894794 1106 36-37 0.02432304111771799 1106 38-39 0.14313476625176236 1106 40-41 0.20501420418170113 1106 42-43 0.330830367060571 1106 44-45 0.29862486285425405 1106 46-47 -0.06127929840337032 1106 48-49 0.2039566574364855 1106 50-51 0.4730896872940704 1106 52-53 0.30097502769500295 1106 54-55 0.367862492226827 1106 56-57 0.4778381983824218 1106 58-59 0.5688543456644553 1106 60-61 0.6853776612236047 1106 62-63 0.6278884175267407 1106 64-65 0.7996042583675766 1106 66-67 0.7296312121288224 1106 68-69 0.6912819398160153 1106 70-71 0.736963242946139 1106 72-73 1.005027806824632 1106 74-75 0.8364231520423182 1106 76-77 0.8938953430174763 1106 78-79 1.0216825052341854 1106 80-81 1.1995059282977927 1106 82-83 1.4949090310724742 1106 84-85 1.6388245480849584 1106 86-87 1.531734707973591 1106 88-89 1.600854457825175 1106 90-91 1.7071066707390656 1106 92-93 1.4124776599775721 1106 94-95 1.277648177919314 1106 96-97 1.5262267751265952 1106 98-99 1.7307377854381727 1106 100-101 1.4518211243881414 1107 1 0.09753334017180748 1107 2 0.09263749785428388 1107 3 0.07786002458426111 1107 4 0.11297968009011328 1107 5 0.1396869859112968 1107 6 -0.015919740704418928 1107 7 0.12275748062504022 1107 8 0.06042177693773709 1107 9 0.12013588271223341 1107 10-11 0.1529453236511955 1107 12-13 0.10846297860891951 1107 14-15 0.190778172805544 1107 16-17 0.13748335176317283 1107 18-19 0.09686169080437423 1107 20-21 0.17324282144551972 1107 22-23 0.2574816070112007 1107 24-25 0.12799126092507862 1107 26-27 0.25531338661130576 1107 28-29 0.3278510144849349 1107 30-31 0.34630179667092875 1107 32-33 0.3397595953612509 1107 34-35 0.3173025514058523 1107 36-37 0.2904506890245315 1107 38-39 0.283388267367215 1107 40-41 0.33138426097226414 1107 42-43 0.4594246032544831 1107 44-45 0.49200953064814 1107 46-47 0.5919290984575767 1107 48-49 0.611295975866625 1107 50-51 0.6091000566612337 1107 52-53 0.5723616397452389 1107 54-55 0.63560000931178 1107 56-57 0.5367130638839228 1107 58-59 0.6447923039460335 1107 60-61 0.7884564409950272 1107 62-63 0.9451746012589695 1107 64-65 1.0429284797302962 1107 66-67 0.9664334505162522 1107 68-69 1.1401981415367644 1107 70-71 0.9817044034345308 1107 72-73 1.4254728670425685 1107 74-75 1.3586051643476331 1107 76-77 1.4166585965725957 1107 78-79 1.6800025098871068 1107 80-81 1.7196001084073878 1107 82-83 1.7920186946081103 1107 84-85 1.990228899397124 1107 86-87 2.3451505752656345 1107 88-89 2.351782964860643 1107 90-91 2.459699469512689 1107 92-93 2.363515302940648 1107 94-95 2.154252791789176 1107 96-97 2.035578398871447 1107 98-99 2.227339698952001 1107 100-101 1.8049524987862728 1108 1 -0.022001131677427566 1108 2 0.049007866340438966 1108 3 0.08466170851096422 1108 4 0.030996042414166425 1108 5 -0.009053566643054012 1108 6 0.020366399650363576 1108 7 0.05828416876474307 1108 8 0.009565028353463845 1108 9 0.067627798528612 1108 10-11 0.0763644961233183 1108 12-13 0.08274812056304626 1108 14-15 0.20861480711427305 1108 16-17 0.09819175518062906 1108 18-19 -0.023373410291640084 1108 20-21 0.02770740524918125 1108 22-23 0.15434276572828765 1108 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-0.2740004480388549 2316 56-57 -0.2112446004083992 2316 58-59 -0.2135096550121247 2316 60-61 -0.04166277317620626 2316 62-63 -0.14211557256741614 2316 64-65 -0.027706981987886792 2316 66-67 -0.10960303675763683 2316 68-69 0.18370284270492832 2316 70-71 0.24252378611939207 2316 72-73 0.3075801225972121 2316 74-75 0.4740338663125456 2316 76-77 0.65663037908012 2316 78-79 0.43976500476795266 2316 80-81 0.17286294197253937 2316 82-83 0.7297105889440161 2316 84-85 0.6532424930997252 2316 86-87 0.9363817798083005 2316 88-89 0.7219397200654392 2316 90-91 1.0055118908231186 2316 92-93 1.2840425141873375 2316 94-95 1.570679521843747 2316 96-97 1.3279424288731612 2316 98-99 1.0211318506068174 2316 100-101 0.5556258092791282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 19.0 4 43.0 5 121.0 6 216.0 7 593.0 8 1299.0 9 2354.0 10 3707.0 11 5892.0 12 8724.0 13 12310.0 14 17015.0 15 22057.0 16 27425.0 17 33758.0 18 36293.0 19 37550.0 20 41325.0 21 45650.0 22 50120.0 23 56011.0 24 62600.0 25 72263.0 26 83052.0 27 96370.0 28 113421.0 29 132245.0 30 153702.0 31 178120.0 32 208770.0 33 247633.0 34 297507.0 35 358027.0 36 413192.0 37 362610.0 38 112365.0 39 1886.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.326311224965096 18.862801396714072 14.553555524603778 24.25733185371705 2 16.28602350396815 19.91547600951373 38.74920392770465 25.049296558813474 3 19.627551583829565 22.918079915344634 29.177249598627263 28.277118902198538 4 13.812995430263047 16.288201353598375 35.39599987986352 34.502803336275065 5 14.72316942941048 36.44164259883989 33.885032643254135 14.950155328495498 6 34.27117655785315 34.958563531721346 16.63920617695679 14.13105373346872 7 30.470677142001623 31.08379792679623 19.746857869743536 18.69866706145861 8 26.751067729775023 35.588120546828115 18.927288800653834 18.733522922743024 9 26.20600399789214 16.67601571124892 19.339628794895514 37.77835149596343 10-11 25.743458249993704 26.013234429052883 26.9354942933654 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23.570035954924602 26.054586016275355 27.1077196071064 23.26765842169364 38-39 23.12338489629809 26.34239637166963 27.178692782813247 23.35552594921903 40-41 23.50651758239291 26.050941576902375 27.157908086948023 23.28463275375669 42-43 23.356102219856457 26.02013627352275 27.111519810660596 23.5122416959602 44-45 23.331104519052417 26.228239481442696 27.04001732892991 23.40063867057498 46-47 23.31684034137596 26.441301376398048 26.70221920465038 23.539639077575615 48-49 23.546386485737887 26.235380740119606 26.870511377926015 23.34772139621649 50-51 23.460227998413956 26.31647286568893 26.775509510653162 23.44778962524395 52-53 23.53601106226437 26.380727681235438 26.582896751491315 23.50036450500887 54-55 23.36090303802714 26.259999617747553 26.749267577014006 23.629829767211298 56-57 23.304475296329073 26.46203216927352 26.924330095007935 23.309162439389475 58-59 23.560326564936954 26.20301063238206 26.681068887047736 23.555593915633246 60-61 23.44351203837329 26.275289715498 26.886332381848348 23.39486586428036 62-63 23.29050487963447 26.339241156089145 26.77716290415991 23.59309106011648 64-65 23.19313097696699 26.1976687126801 26.78155994050811 23.8276403698448 66-67 23.20742424483747 26.294453280303014 26.66704588806446 23.831076586795056 68-69 23.03605103549039 26.170918410686678 26.845676982333682 23.947353571489245 70-71 23.065463662706257 26.17375480034348 26.686580213840067 24.0742013231102 72-73 23.22495288955443 26.130320772722037 26.50294190947968 24.14178442824385 74-75 23.04524139696886 26.333461863189424 26.425551330890762 24.195745408950955 76-77 23.119113198600473 26.222745173228617 26.61329492201898 24.04484670615193 78-79 23.148677088000884 26.21808836766375 26.690200473204257 23.943034071131112 80-81 22.939607216875316 26.430350400068686 26.640680543763946 23.989361839292055 82-83 23.097929713844167 26.31272694618154 26.474640124676483 24.114703215297805 84-85 23.05369950648571 26.075054280807553 26.48076662567321 24.39047958703353 86-87 22.998817091525687 25.930694730429448 26.710218976109136 24.360269201935736 88-89 22.914835672404802 26.183351935274345 26.48894152781448 24.412870864506374 90-91 22.999973454381355 26.12337645100248 26.584875823388206 24.29177427122796 92-93 22.886748062562287 25.833686008430828 26.59341642813695 24.68614950086993 94-95 22.755168071344126 25.88619670419316 26.564953072416557 24.79368215204616 96-97 22.72280621918847 25.917618505877893 26.47142965491088 24.888145620022755 98-99 22.74179059786406 25.79994666668285 26.316427359445626 25.14183537600747 100-101 22.888418657565417 25.570830489192264 26.34351156617368 25.19723928706864 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 183.0 1 139.5 2 123.5 3 156.5 4 152.5 5 167.0 6 292.5 7 659.5 8 1002.0 9 1133.5 10 1079.0 11 995.5 12 1129.0 13 1292.5 14 1455.5 15 1591.5 16 1715.5 17 1860.5 18 2026.0 19 2228.5 20 2449.0 21 2886.5 22 3475.5 23 4241.5 24 5380.5 25 6917.0 26 9095.5 27 11937.0 28 14834.0 29 18511.5 30 23605.5 31 28711.0 32 34132.0 33 39950.0 34 46010.0 35 52404.5 36 59391.5 37 67331.5 38 74774.0 39 80186.5 40 86700.0 41 97667.5 42 108235.0 43 117142.5 44 125893.0 45 132142.5 46 135503.5 47 140197.5 48 144193.5 49 142967.5 50 140108.5 51 135796.5 52 130155.0 53 126790.0 54 126152.5 55 119278.0 56 107106.5 57 97298.0 58 88622.0 59 78413.0 60 65260.5 61 52904.5 62 42899.5 63 34423.0 64 28071.0 65 22423.5 66 16469.5 67 12806.0 68 9870.0 69 6615.5 70 4761.0 71 3592.0 72 2773.0 73 2176.0 74 1770.0 75 1419.0 76 934.0 77 532.0 78 299.0 79 165.5 80 97.5 81 54.5 82 25.0 83 14.0 84 8.0 85 3.5 86 3.0 87 1.5 88 0.0 89 0.0 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024330671420349965 2 0.011588923294979661 3 0.001911262218805546 4 0.0 5 3.337124509025557E-4 6 0.0 7 0.0 8 1.5168747768297983E-4 9 4.2472493751234355E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017899122366591622 26-27 0.009389454868576452 28-29 1.0618123437808589E-4 30-31 0.0 32-33 2.5786871206106573E-4 34-35 0.009874854797161988 36-37 0.0012286685692321367 38-39 0.012438373170004348 40-41 0.0120439857280286 42-43 0.004838830538087057 44-45 8.797873705612832E-4 46-47 7.584373884148991E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.0618123437808589E-4 66-67 7.129311451100053E-4 68-69 0.001061812343780859 70-71 7.584373884148991E-5 72-73 2.2753121652446977E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.3651872991468186E-4 82-83 0.0012134998214638386 84-85 0.005961317872941108 86-87 0.004186574384050244 88-89 0.0 90-91 0.0011679935781589447 92-93 4.853999285855355E-4 94-95 6.067499107319194E-5 96-97 3.033749553659597E-5 98-99 0.0 100-101 3.033749553659597E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3296251.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 47.49991064391698 #Duplication Level Percentage of deduplicated Percentage of total 1 72.54525810025407 34.45893277401963 2 14.385637587670566 13.666329999402507 3 5.112496360936042 7.2852936093543805 4 2.47639630762463 4.705144133243834 5 1.364720901354912 3.2412060434122076 6 0.8433222391333496 2.4034638601717253 7 0.575080636815317 1.91213952032421 8 0.4342422488169306 1.6501174413294213 9 0.3073938890512337 1.3141064036177756 >10 1.7267415458783715 15.012794705885229 >50 0.13852348447793097 4.578502784471104 >100 0.08537600661376289 7.532778909731768 >500 0.0035919828923292032 1.1335490936391759 >1k 0.0012187084805983584 1.1056407213969521 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4528 0.13736817978970656 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3835 0.11634429538284555 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 3.033749553659597E-5 0.0 20-21 0.0 0.0 0.0 1.061812343780859E-4 0.0 22-23 0.0 0.0 0.0 1.5168747768297985E-4 0.0 24-25 0.0 0.0 0.0 3.4888119867085364E-4 0.0 26-27 0.0 0.0 0.0 6.067499107319193E-4 0.0 28-29 0.0 0.0 0.0 0.0024269996429276777 0.0 30-31 0.0 0.0 0.0 0.008843379948917724 0.0 32-33 0.0 0.0 0.0 0.02387560898730103 0.0 34-35 0.0 0.0 0.0 0.05034507384298101 0.0 36-37 0.0 0.0 0.0 0.09299959256743494 0.0 38-39 0.0 0.0 0.0 0.15857408916978716 0.0 40-41 0.0 0.0 0.0 0.23152059718753215 0.0 42-43 0.0 0.0 0.0 0.30649971740622906 0.0 44-45 0.0 0.0 0.0 0.4005459535696766 0.0 46-47 0.0 0.0 0.0 0.5165110302583147 0.0 48-49 0.0 0.0 0.0 0.6380126998823816 0.0 50-51 0.0 0.0 0.0 0.7640194875936328 0.0 52-53 0.0 0.0 0.0 0.8904510002423967 0.0 54-55 0.0 0.0 0.0 1.0339018478871906 0.0 56-57 0.0 0.0 0.0 1.1999541297067486 0.0 58-59 0.0 0.0 0.0 1.3802498656807387 0.0 60-61 0.0 0.0 0.0 1.5714974375434396 0.0 62-63 0.0 0.0 0.0 1.7703748895335942 0.0 64-65 0.0 0.0 0.0 1.9793395587896674 0.0 66-67 0.0 0.0 0.0 2.214576499180432 1.5168747768297985E-5 68-69 0.0 0.0 0.0 2.4771475230496707 6.067499107319194E-5 70-71 0.0 0.0 0.0 2.760135681415038 9.101248660978791E-5 72-73 0.0 0.0 0.0 3.0489486389234317 9.101248660978791E-5 74-75 0.0 0.0 0.0 3.345072932856145 1.8202497321957581E-4 76-77 0.0 0.0 0.0 3.6686071540061724 2.1236246875617177E-4 78-79 0.0 0.0 0.0 4.024329457920529 2.1236246875617177E-4 80-81 0.0 0.0 0.0 4.406217851735199 2.4269996429276776E-4 82-83 0.0 3.033749553659597E-5 0.0 4.796176019362603 2.4269996429276776E-4 84-85 0.0 3.033749553659597E-5 0.0 5.190032555166461 2.4269996429276776E-4 86-87 0.0 3.033749553659597E-5 0.0 5.601666863354763 2.4269996429276776E-4 88-89 0.0 3.033749553659597E-5 0.0 6.045322398081943 2.4269996429276776E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4755 0.0 38.17521 1 GTATCAA 6600 0.0 34.703373 1 ATCAACG 8190 0.0 27.373417 3 TCAACGC 8305 0.0 26.879587 4 TATCAAC 8525 0.0 26.470915 2 CAACGCA 8525 0.0 26.241632 5 GTACATG 9145 0.0 25.773096 1 AACGCAG 8895 0.0 25.310268 6 TACATGG 9155 0.0 25.064472 2 ACATGGG 9725 0.0 22.662054 3 GGACCGA 1020 0.0 21.88578 6 ACGCAGA 10300 0.0 21.442728 7 CGCAGAG 10580 0.0 20.830355 8 AGGACCG 1170 0.0 19.891823 5 CATGGGG 6925 0.0 19.82177 4 ACCGAGT 1150 0.0 18.99872 8 GAGTACT 5740 0.0 18.990444 12-13 ATTCGCG 630 0.0 18.850225 80-81 AATTCGC 645 0.0 18.778944 78-79 TCGCGGA 615 0.0 18.537586 82-83 >>END_MODULE