FastQCFastQC Report
Thu 26 May 2016
SRR938535_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938535_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971285
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65280.21970292314604625No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.17837400316697993No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35470.11937596023269394No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46000.048.4885861
GTATCAA77200.041.2745171
ATCAACG96900.032.3445243
TCAACGC98650.031.867174
TATCAAC99400.031.6267222
CAACGCA100100.031.4055585
AACGCAG104900.030.0358896
GTACATG95700.026.5371191
TACATGG96200.025.8068262
CGCAGAG122400.025.4262228
ACGCAGA122800.025.4207847
ACATGGG97850.024.7883993
GCAGAGT134400.022.3425359
CATGGGG73100.021.7304254
GAGTACT74100.020.45269412-13
GGACCGA9900.019.681186
CAGAGTA129900.019.65968910-11
AGAGTAC122950.019.61168110-11
GTACTTT77500.019.27922814-15
AGTACTT78050.019.14369612-13
Produced by FastQC (version 0.11.5)