FastQCFastQC Report
Thu 26 May 2016
SRR938535_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938535_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2971285
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65750.2212847303439421No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57810.19456228534119077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38540.12970819022746052No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.046.9414251
GTATCAA78000.039.2483981
ATCAACG98400.030.6566033
TCAACGC101100.029.884874
TATCAAC101050.029.8722572
CAACGCA103500.029.3295865
AACGCAG108400.027.9906066
ACGCAGA122950.024.4850757
CGCAGAG124500.024.1039628
GTACATG94050.023.6547151
TACATGG95000.022.7176252
TGGTATC25200.021.5047782
ACATGGG97450.021.3519573
GCAGAGT138000.021.3330679
GTGGTAT26000.020.8430941
GAGTACT79350.020.10634212-13
GTACTTT80200.019.80443614-15
AGAGTAC125550.019.3072210-11
CAGAGTA132050.019.09399610-11
AGTACTT83750.018.48304412-13
Produced by FastQC (version 0.11.5)