##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938535_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2971285 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78117514812615 31.0 31.0 34.0 30.0 34.0 2 32.00325583038988 31.0 31.0 34.0 30.0 34.0 3 32.06923603760662 33.0 31.0 34.0 30.0 34.0 4 35.74226201794846 37.0 35.0 37.0 33.0 37.0 5 35.63704289558221 37.0 35.0 37.0 33.0 37.0 6 35.635969959125426 37.0 35.0 37.0 33.0 37.0 7 35.57542107202776 37.0 35.0 37.0 33.0 37.0 8 35.5517420913847 37.0 35.0 37.0 33.0 37.0 9 37.13477165603434 39.0 37.0 39.0 33.0 39.0 10-11 37.030847259687306 39.0 37.0 39.0 33.0 39.0 12-13 36.91683026030825 39.0 37.0 39.0 33.0 39.0 14-15 37.964832892166186 40.0 37.0 41.0 33.0 41.0 16-17 37.863569129181485 40.0 37.0 41.0 33.0 41.0 18-19 38.09144949070857 40.0 37.0 41.0 33.0 41.0 20-21 38.05727774346789 40.0 37.0 41.0 33.0 41.0 22-23 37.94485281620578 40.0 37.0 41.0 32.5 41.0 24-25 37.81875568987829 40.0 37.0 41.0 32.0 41.0 26-27 37.62311777564253 40.0 37.0 41.0 32.0 41.0 28-29 37.5097074161516 39.5 36.5 41.0 32.0 41.0 30-31 37.273513648135406 39.0 36.0 41.0 31.0 41.0 32-33 37.09090444033474 39.0 36.0 41.0 31.0 41.0 34-35 37.006423651719714 39.0 36.0 41.0 30.5 41.0 36-37 36.98110228402862 39.0 36.0 41.0 31.0 41.0 38-39 36.826579914077584 39.0 35.0 41.0 30.0 41.0 40-41 36.663001361363854 39.0 35.0 40.5 30.0 41.0 42-43 36.461902341916044 39.0 35.0 40.0 30.0 41.0 44-45 36.179469657067564 38.5 35.0 40.0 30.0 41.0 46-47 35.81719996567142 38.0 35.0 40.0 28.5 41.0 48-49 35.48237513399085 38.0 34.0 40.0 27.5 41.0 50-51 35.10354324812329 38.0 34.0 40.0 26.5 41.0 52-53 34.59989432181699 38.0 33.0 40.0 25.5 41.0 54-55 34.24410583299818 37.0 33.0 40.0 24.0 41.0 56-57 33.89286790058847 37.0 33.0 40.0 24.0 41.0 58-59 33.39503750061 36.5 32.0 39.0 23.0 40.5 60-61 32.97263271614807 36.0 31.0 39.0 22.0 40.0 62-63 32.469333638476286 35.5 31.0 39.0 20.5 40.0 64-65 31.914386704742228 35.0 30.5 38.0 18.5 40.0 66-67 31.386231378006485 35.0 30.0 38.0 17.5 40.0 68-69 30.868067183053796 34.0 29.0 37.0 13.5 39.5 70-71 30.26873002758066 34.0 29.0 37.0 9.5 39.0 72-73 29.733155520254705 34.0 28.0 36.0 7.5 39.0 74-75 29.11734603042118 33.5 27.5 35.5 7.0 38.5 76-77 27.914564237358583 31.5 26.0 34.5 3.5 36.0 78-79 28.592694911460868 33.0 27.0 35.0 2.0 37.0 80-81 28.204203400212364 33.0 26.5 35.0 2.0 36.5 82-83 27.735766175240677 33.0 26.0 35.0 2.0 36.0 84-85 27.15933341971571 32.0 25.5 35.0 2.0 36.0 86-87 26.507794102551593 32.0 24.5 34.5 2.0 35.0 88-89 25.72726211050101 31.0 22.5 34.0 2.0 35.0 90-91 25.080779864604033 31.0 20.0 34.0 2.0 35.0 92-93 24.195209480073437 31.0 16.5 34.0 2.0 35.0 94-95 23.335467482924052 30.0 4.5 34.0 2.0 35.0 96-97 22.206262610284774 30.0 2.0 34.0 2.0 35.0 98-99 20.784818016447428 29.0 2.0 34.0 2.0 35.0 100-101 16.880254166126775 22.0 2.0 31.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.25704194810053593 1101 2 0.27375133708159893 1101 3 0.2807904806197641 1101 4 0.20712266412984803 1101 5 0.14664453318162884 1101 6 0.2482443928271394 1101 7 0.26104101974544136 1101 8 0.2201059943992476 1101 9 0.300349843992862 1101 10-11 0.23640231451300764 1101 12-13 0.37026865209638515 1101 14-15 0.4038614378384011 1101 16-17 0.4568005361727572 1101 18-19 0.39201066122279116 1101 20-21 0.30305367096288904 1101 22-23 0.3472879088848586 1101 24-25 0.4472218316443204 1101 26-27 0.39903310763614286 1101 28-29 0.39176944234746713 1101 30-31 0.45725905183953586 1101 32-33 0.34918325194717426 1101 34-35 0.46041993649721746 1101 36-37 0.3792396399625346 1101 38-39 0.4317443483512733 1101 40-41 0.5735798439050797 1101 42-43 0.6190497427474853 1101 44-45 0.5800762913632767 1101 46-47 0.5327294639602442 1101 48-49 0.5448340512012706 1101 50-51 0.7005778968013558 1101 52-53 0.5840991281059971 1101 54-55 0.744202450515921 1101 56-57 0.750586337140966 1101 58-59 0.8530175063667826 1101 60-61 0.9225478606216839 1101 62-63 0.9404956139797349 1101 64-65 0.7743404858954506 1101 66-67 0.6505538663356383 1101 68-69 0.7166069240020079 1101 70-71 0.6059909445994407 1101 72-73 0.5985884808867006 1101 74-75 0.5591050726808291 1101 76-77 0.6543896060767374 1101 78-79 0.8424289748390024 1101 80-81 0.8855510206706896 1101 82-83 0.7249394625552199 1101 84-85 0.8745501724180009 1101 86-87 0.7117724475107607 1101 88-89 0.7456814289084726 1101 90-91 0.822620431065122 1101 92-93 1.2722044216478174 1101 94-95 1.2088186069548215 1101 96-97 1.4892596503017081 1101 98-99 1.6013602958958124 1101 100-101 1.5301917207363225 1102 1 0.20999163747517002 1102 2 0.25212033327935757 1102 3 0.20407450580010789 1102 4 0.2294919741270931 1102 5 0.08921096912653326 1102 6 0.1358551452078629 1102 7 0.022558570581729498 1102 8 0.16608422559205138 1102 9 0.17041027620004456 1102 10-11 0.28688196838391633 1102 12-13 0.3154312884869057 1102 14-15 0.33490851653549925 1102 16-17 0.38588159398670285 1102 18-19 0.3422503462259101 1102 20-21 0.3100442254951048 1102 22-23 0.40850874372568313 1102 24-25 0.3169667869820856 1102 26-27 0.28954392468639867 1102 28-29 0.2750938382447501 1102 30-31 0.355444666300734 1102 32-33 0.29544508974896644 1102 34-35 0.19266974979167628 1102 36-37 0.30662357415135233 1102 38-39 0.3618523511156013 1102 40-41 0.32840799481579097 1102 42-43 0.209182968177835 1102 44-45 0.33921879813603084 1102 46-47 0.4101619906190024 1102 48-49 0.3979073765416601 1102 50-51 0.5136752061255905 1102 52-53 0.463198327452794 1102 54-55 0.33292035251860597 1102 56-57 0.3637519546266148 1102 58-59 0.5027323772109398 1102 60-61 0.38077433611152145 1102 62-63 0.4160272537936649 1102 64-65 0.5090152776291035 1102 66-67 0.4436299425827208 1102 68-69 0.58293621321004 1102 70-71 0.508379433525878 1102 72-73 0.5939796799465427 1102 74-75 0.7121270959339903 1102 76-77 0.6571276098268477 1102 78-79 0.8215231162335392 1102 80-81 0.8644529082675092 1102 82-83 0.6694364362998044 1102 84-85 0.8322911102965378 1102 86-87 0.8846431915406967 1102 88-89 0.8574047558230227 1102 90-91 0.7430259394848058 1102 92-93 1.1589424192045144 1102 94-95 1.180490461463343 1102 96-97 1.2123984399956633 1102 98-99 1.339673974611589 1102 100-101 1.4903366052627582 1103 1 0.22804517711725936 1103 2 0.2534765506447201 1103 3 0.18114814473425866 1103 4 0.12218095083088798 1103 5 0.04775919025703956 1103 6 0.0728402637518073 1103 7 0.1373183512448577 1103 8 0.15643839362267897 1103 9 0.241486738991469 1103 10-11 0.23067084120039283 1103 12-13 0.3566168638385605 1103 14-15 0.43714666430253857 1103 16-17 0.409338570927666 1103 18-19 0.4140708818199741 1103 20-21 0.3670737277624596 1103 22-23 0.3504282625581894 1103 24-25 0.42710629986052595 1103 26-27 0.45087746225321723 1103 28-29 0.3729086841246456 1103 30-31 0.3192055352483081 1103 32-33 0.32280006441948217 1103 34-35 0.43128204505893564 1103 36-37 0.47343324543015086 1103 38-39 0.38953634345190125 1103 40-41 0.5059485090147575 1103 42-43 0.619769777083242 1103 44-45 0.46551371457530166 1103 46-47 0.47106101733941586 1103 48-49 0.5356605941454333 1103 50-51 0.6917767241650452 1103 52-53 0.5423828799900079 1103 54-55 0.5148926920584174 1103 56-57 0.6560476171769096 1103 58-59 0.6771886982165611 1103 60-61 0.8487442470846602 1103 62-63 0.790944953054094 1103 64-65 0.7997949389391721 1103 66-67 0.8012131605200175 1103 68-69 0.7140754777965697 1103 70-71 0.6073660689975284 1103 72-73 0.6405518309821403 1103 74-75 0.718378863932994 1103 76-77 0.5990799254268673 1103 78-79 0.8660826204402952 1103 80-81 0.9891766680741085 1103 82-83 1.1700063959431048 1103 84-85 1.03492745158551 1103 86-87 1.1208733050261586 1103 88-89 1.0218628185778762 1103 90-91 1.290427397860089 1103 92-93 1.4827131580644242 1103 94-95 1.4246755513487201 1103 96-97 1.340769620345771 1103 98-99 1.6968787017931284 1103 100-101 2.040089980982774 1104 1 0.16284769344616734 1104 2 0.19153082490961282 1104 3 0.17444841317227855 1104 4 0.14515314523283962 1104 5 0.14158453141406824 1104 6 0.06095279502770268 1104 7 0.14830058665459944 1104 8 0.17211239276488755 1104 9 0.07785453998504721 1104 10-11 0.12117033225499085 1104 12-13 0.2119729444798253 1104 14-15 0.2739780087948773 1104 16-17 0.26224937634690804 1104 18-19 0.2439692329771148 1104 20-21 0.1879274004083129 1104 22-23 0.30909961705414446 1104 24-25 0.36761420516747023 1104 26-27 0.22196392921382824 1104 28-29 0.29086896417900476 1104 30-31 0.25803912120398564 1104 32-33 0.39406299695003355 1104 34-35 0.42171895511174995 1104 36-37 0.3611928455661868 1104 38-39 0.3063240039508486 1104 40-41 0.42783526486471146 1104 42-43 0.45537982790766307 1104 44-45 0.5182957351878841 1104 46-47 0.37017074269588335 1104 48-49 0.47074931191855995 1104 50-51 0.6348359593328823 1104 52-53 0.4501222391745756 1104 54-55 0.42775434422024006 1104 56-57 0.4286400015773495 1104 58-59 0.4305046732589517 1104 60-61 0.6009165849963622 1104 62-63 0.527433358331237 1104 64-65 0.5479220081732734 1104 66-67 0.504165861904049 1104 68-69 0.49569168037437095 1104 70-71 0.3657747702693648 1104 72-73 0.3407711858660889 1104 74-75 0.5367569349890147 1104 76-77 0.5766701419886857 1104 78-79 0.7534890557072345 1104 80-81 0.6092141582428745 1104 82-83 0.9758952623680166 1104 84-85 0.9560947888602982 1104 86-87 1.095786445400428 1104 88-89 1.051519669006364 1104 90-91 0.9621272871893005 1104 92-93 1.1705532194884647 1104 94-95 1.2999597953106168 1104 96-97 1.1659592039688285 1104 98-99 1.407009695341035 1104 100-101 1.4202945174334296 1105 1 0.13570232221993095 1105 2 0.15758311880753695 1105 3 0.13753961664603764 1105 4 0.0439097997886293 1105 5 0.04748584668728739 1105 6 0.09951813384484609 1105 7 0.14224327422611793 1105 8 0.14731912047385975 1105 9 0.17209746249664448 1105 10-11 0.15302664668841004 1105 12-13 0.10316785166061493 1105 14-15 0.27299425763664686 1105 16-17 0.20078598002937298 1105 18-19 0.2722419186127141 1105 20-21 0.20110205494026445 1105 22-23 0.17033651620484136 1105 24-25 0.2512855323372136 1105 26-27 0.16241851319855982 1105 28-29 0.15283492181334424 1105 30-31 0.2230877652001766 1105 32-33 0.23698630674289234 1105 34-35 0.2577632580340321 1105 36-37 0.27821519758867197 1105 38-39 0.3128849119811079 1105 40-41 0.4095276338876914 1105 42-43 0.38580212871055863 1105 44-45 0.25913573647399346 1105 46-47 0.2151344606535659 1105 48-49 0.2988962307498966 1105 50-51 0.41937506283715464 1105 52-53 0.45761990585015866 1105 54-55 0.5726441782557217 1105 56-57 0.4585353903976781 1105 58-59 0.5385466436437767 1105 60-61 0.7504502461380653 1105 62-63 0.6572724348316754 1105 64-65 0.50368890012669 1105 66-67 0.42342541729192007 1105 68-69 0.48997483881985815 1105 70-71 0.5770130859407843 1105 72-73 0.600418984851455 1105 74-75 0.6394236027049587 1105 76-77 0.7078228166158986 1105 78-79 1.0262261807142892 1105 80-81 1.0352622205661888 1105 82-83 1.1360027448127177 1105 84-85 1.0627136056068096 1105 86-87 1.0077184290218533 1105 88-89 1.0947521063416197 1105 90-91 1.1915419808262193 1105 92-93 1.3291876328026824 1105 94-95 1.1820457434924876 1105 96-97 1.367531227189879 1105 98-99 1.5482049959998072 1105 100-101 1.4725624458078492 1106 1 -0.003864598096789962 1106 2 0.09099663364968791 1106 3 0.09786381537010413 1106 4 0.08147345422955254 1106 5 0.05272684188911825 1106 6 0.04547679225889567 1106 7 0.10157333283603265 1106 8 0.16476130822356083 1106 9 -0.050150970784322624 1106 10-11 0.17967394813826587 1106 12-13 0.04293915187842856 1106 14-15 0.05721271188093624 1106 16-17 0.1784450891740974 1106 18-19 0.05760508831494349 1106 20-21 0.1411263416428028 1106 22-23 0.28842142786970015 1106 24-25 0.2477276299518394 1106 26-27 0.18276338216261934 1106 28-29 0.265151686329105 1106 30-31 0.21336213526382863 1106 32-33 0.22744581314552903 1106 34-35 0.23106159905096035 1106 36-37 0.36240749607180334 1106 38-39 0.26788118768826763 1106 40-41 0.19083516757636687 1106 42-43 0.43331326702809037 1106 44-45 0.2699634474246295 1106 46-47 -0.4000864228139491 1106 48-49 -0.09520357145061098 1106 50-51 0.3886105791535286 1106 52-53 0.2374410955557309 1106 54-55 0.3683463940855418 1106 56-57 0.3359082792047232 1106 58-59 0.030156671751541353 1106 60-61 0.15364406917581874 1106 62-63 -0.047741453158621994 1106 64-65 0.40298407701539674 1106 66-67 0.3418924906170808 1106 68-69 -0.08466507708356374 1106 70-71 0.007014849933977274 1106 72-73 0.36244065376596524 1106 74-75 0.3108658800367614 1106 76-77 0.16572503609536327 1106 78-79 0.4484636097743895 1106 80-81 0.09321022645206511 1106 82-83 0.4367923391329924 1106 84-85 0.8120447535792934 1106 86-87 0.9207054556537244 1106 88-89 1.063832347018863 1106 90-91 1.0945632753760357 1106 92-93 1.168000183365418 1106 94-95 1.3900589175908777 1106 96-97 1.4237481419491935 1106 98-99 1.5085152044330208 1106 100-101 1.332831070451661 1107 1 0.08430470862629846 1107 2 0.0934298697679452 1107 3 0.09344243567852573 1107 4 0.05031221063907765 1107 5 -0.028212331973371363 1107 6 0.09402428468183643 1107 7 0.014340912561927155 1107 8 0.04710819448192183 1107 9 -0.016031339057896332 1107 10-11 0.05508472948059051 1107 12-13 0.051475493160531016 1107 14-15 0.16398202622873725 1107 16-17 0.16589243100899154 1107 18-19 0.08180258740950563 1107 20-21 0.1053273671665238 1107 22-23 0.1473771282947851 1107 24-25 0.1763493680048711 1107 26-27 0.16729141250897328 1107 28-29 0.13118264265856538 1107 30-31 0.09898717100465859 1107 32-33 0.02995624946964881 1107 34-35 0.12431754156064301 1107 36-37 0.1948058491340774 1107 38-39 0.20039152870349142 1107 40-41 0.22983842714061353 1107 42-43 0.11622056959570415 1107 44-45 0.2439269631791916 1107 46-47 0.3206880572760227 1107 48-49 0.23211481744780116 1107 50-51 0.27816329450398314 1107 52-53 0.3396022247755397 1107 54-55 0.18699024380247664 1107 56-57 0.18627807772171678 1107 58-59 0.21346808403876594 1107 60-61 0.20202286485260856 1107 62-63 0.3768596791110639 1107 64-65 0.26239678359743124 1107 66-67 0.20096780370680278 1107 68-69 0.3687417394086907 1107 70-71 0.6569152521375194 1107 72-73 0.6869726870859587 1107 74-75 0.5892159877358125 1107 76-77 0.4541011631230596 1107 78-79 0.7259707102871502 1107 80-81 0.6780994775147562 1107 82-83 0.889078323006725 1107 84-85 0.9342622103567102 1107 86-87 1.0487910811660086 1107 88-89 0.989414684374232 1107 90-91 1.1200226642553908 1107 92-93 1.523155918101697 1107 94-95 1.6512396041961637 1107 96-97 1.5167410319055428 1107 98-99 1.506300702231389 1107 100-101 1.41515937147528 1108 1 0.1264354520401234 1108 2 0.15121299370984786 1108 3 0.13722900492619772 1108 4 0.10196209629056341 1108 5 0.047844277964550486 1108 6 0.19016613623668377 1108 7 0.13047147777027845 1108 8 0.13046380349911857 1108 9 0.14632498278118078 1108 10-11 0.19981356015569673 1108 12-13 0.2367033734064563 1108 14-15 0.3163588416969745 1108 16-17 0.26967112951502514 1108 18-19 0.28478604400162766 1108 20-21 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-0.8607855575409431 2316 72-73 -0.9517167935801325 2316 74-75 -0.7799739554492504 2316 76-77 -0.9561218961117994 2316 78-79 -1.0775814972178566 2316 80-81 -1.0293653779924625 2316 82-83 -1.125841388640879 2316 84-85 -1.2325530955466988 2316 86-87 -1.3638491995266406 2316 88-89 -1.4470769817905342 2316 90-91 -1.3309178939486443 2316 92-93 -1.8079868203503864 2316 94-95 -1.6384584612545154 2316 96-97 -1.6147060502097226 2316 98-99 -1.7926523756432182 2316 100-101 -2.195508138381239 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 15.0 4 42.0 5 109.0 6 233.0 7 555.0 8 1264.0 9 2204.0 10 3606.0 11 5527.0 12 7957.0 13 11327.0 14 14712.0 15 19462.0 16 23560.0 17 29107.0 18 29354.0 19 27768.0 20 28123.0 21 30383.0 22 33600.0 23 37932.0 24 43009.0 25 51101.0 26 59403.0 27 71372.0 28 84970.0 29 99649.0 30 118217.0 31 139727.0 32 166071.0 33 202104.0 34 253938.0 35 324388.0 36 415294.0 37 439660.0 38 189166.0 39 6370.0 >>END_MODULE >>Per base sequence content fail #Base G A 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23.106837614028947 18-19 23.102529713575105 26.58346472990642 27.76934895171618 22.5446566048023 20-21 23.49052682593558 25.81374725076861 28.031979429775333 22.66374649352048 22-23 23.3532461544416 25.602071157765078 27.884517977911916 23.160164709881414 24-25 23.226358543370225 25.844694145780778 27.93721527849066 22.991732032358332 26-27 22.886862711705824 26.213995453722404 27.79632420600009 23.102817628571678 28-29 22.932067213400273 26.360418438839123 27.80309512811387 22.904419219646734 30-31 23.059011646300338 26.021272571353954 27.75475524729709 23.16496053504862 32-33 22.937868318934328 25.989711470179415 27.75294739286568 23.319472818020575 34-35 23.40601026579693 26.130961867709257 27.528132151056223 22.93489571543759 36-37 23.446509834376634 25.8712853572421 27.54877718514165 23.133427623239616 38-39 23.092902380256003 26.1025945837152 27.557165556378653 23.24733747965014 40-41 23.381623895666806 25.905814051325198 27.581455616323098 23.1311064366849 42-43 23.18189604949582 25.929737776235584 27.497702138601344 23.390664035667253 44-45 23.216975349883427 26.10015412002648 27.51446368822451 23.168406841865583 46-47 23.338538713048397 26.400294821937308 26.997140967628486 23.264025497385813 48-49 23.33362501409323 26.19095778425833 27.09997526322786 23.375441938420582 50-51 23.399509872800163 26.415510272629255 26.9715857144564 23.213394140114183 52-53 23.5969286015983 26.285815732923634 26.80824962936911 23.309006036108958 54-55 23.204478926780755 26.310329247226967 27.03860920370989 23.446582622282392 56-57 23.205952979939656 26.505417689652795 27.011511854298732 23.27711747610882 58-59 23.37899880008436 26.242402483921317 26.92860476690852 23.44999394908581 60-61 23.230841201702294 26.343164657715434 27.11668520522266 23.309308935359617 62-63 23.13696702388297 26.42978240872102 27.109479577614355 23.323770989781657 64-65 22.934996206462678 26.27241704540329 27.107920919389183 23.684665828744848 66-67 22.939416678601056 26.34659848107094 27.0063358550089 23.707648985319103 68-69 22.98204427072595 26.13555214873127 27.20871862771218 23.67368495283059 70-71 22.985671334069156 26.192873344380196 26.969810837512366 23.851644484038285 72-73 23.06502457102307 26.297700539951663 26.785704970358783 23.851569918666492 74-75 22.9634305007835 26.58432764601627 26.654514423635273 23.79772742956496 76-77 23.04201743204974 26.301027198287336 26.84835672349118 23.80859864617175 78-79 23.077820384871046 26.350973186146685 26.873221949664757 23.697984479317512 80-81 23.009097866829556 26.537490008101244 26.73087290944398 23.72253921562522 82-83 23.063535221519476 26.527712407474368 26.68981953756206 23.718932833444097 84-85 23.054414656526372 26.393404051494958 26.676925220828757 23.875256071149913 86-87 23.01741355993131 26.403071270520744 26.6764792404489 23.90303592909905 88-89 22.976655560853125 26.481310790266516 26.49321769544629 24.04881595343406 90-91 22.933003340104207 26.499394391506538 26.602859522394052 23.964742745995206 92-93 22.956510703418477 26.206467866853522 26.65992951575662 24.177091913971385 94-95 22.79899459171405 26.19540355447816 26.69315909308577 24.31244276072202 96-97 22.862094918795638 26.057999963975387 26.672606799931923 24.407298317297048 98-99 22.731457333743244 26.153081782057065 26.42313736569196 24.69232351850773 100-101 22.8884452127152 25.988244918897074 26.625361361116074 24.49794850727165 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 366.0 1 271.0 2 256.0 3 351.0 4 294.5 5 274.5 6 535.5 7 1150.5 8 1831.5 9 2112.5 10 1884.5 11 1624.0 12 1667.0 13 1808.5 14 1993.5 15 2194.5 16 2377.0 17 2600.0 18 2786.0 19 3022.0 20 3410.5 21 3994.0 22 4786.5 23 5836.5 24 7213.5 25 8929.0 26 11489.0 27 14952.5 28 17974.5 29 21172.0 30 25331.0 31 30361.0 32 37125.5 33 41548.5 34 44091.5 35 48623.5 36 54815.0 37 61401.0 38 66775.5 39 71141.0 40 75892.0 41 83325.5 42 90391.5 43 96142.0 44 102605.5 45 108928.5 46 112434.5 47 115592.5 48 118635.0 49 118187.0 50 117411.0 51 115394.0 52 113632.5 53 114669.5 54 116573.0 55 111986.0 56 101751.5 57 93107.5 58 85624.5 59 75741.5 60 62663.5 61 51553.5 62 42957.5 63 33731.0 64 25808.5 65 19839.0 66 14512.0 67 11171.0 68 8435.5 69 5926.5 70 4404.5 71 3208.5 72 2369.5 73 1897.0 74 1508.0 75 1179.0 76 813.0 77 460.5 78 257.5 79 158.5 80 95.5 81 49.5 82 31.0 83 21.0 84 10.0 85 4.5 86 4.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.028992506609093E-4 2 0.050415897498893573 3 0.0 4 3.365547229565659E-5 5 0.04523295476536246 6 3.365547229565659E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006057985013218186 26-27 0.0021202947546263653 28-29 8.413868073914148E-5 30-31 0.0013798743641219202 32-33 5.04832084434849E-4 34-35 0.011829898511923294 36-37 0.02219578397898552 38-39 0.004509833287617984 40-41 0.0011779415303479809 42-43 0.0014808407810088901 44-45 0.007538825794227076 46-47 0.0 48-49 0.0 50-51 5.216598205826773E-4 52-53 0.0 54-55 0.0012620802110871222 56-57 0.0 58-59 0.02219578397898552 60-61 0.0 62-63 0.008615800907688088 64-65 0.01490937422697587 66-67 0.006007501804774702 68-69 0.015161790269193294 70-71 1.1779415303479807E-4 72-73 1.6827736147828296E-5 74-75 0.021539502269220218 76-77 0.04747104367302363 78-79 0.028994189382708157 80-81 0.025359398374777242 82-83 0.03456417004763932 84-85 0.014505508559427992 86-87 0.008986011102940311 88-89 0.08142941521934112 90-91 0.02419828458057709 92-93 0.023289586828594364 94-95 0.025258431957890272 96-97 0.03680225895530048 98-99 0.05135825072317196 100-101 0.013445361182114809 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2971285.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.568002838950555 #Duplication Level Percentage of deduplicated Percentage of total 1 75.92497182950201 40.67149106510006 2 13.309538925922237 14.259308379378508 3 4.408295152220273 7.084307016872028 4 1.9723659028678613 4.226228091370995 5 1.1074729702666515 2.9662557607652493 6 0.6793760249706216 2.183569010060472 7 0.47948600278483633 1.797957529089062 8 0.35156953741698854 1.506630238275342 9 0.245437858038025 1.183285428855018 >10 1.3717945655348813 13.331826377865177 >50 0.09657287794333884 3.5996804206167203 >100 0.048778737991060604 4.659640666232261 >500 0.0029559693252914536 1.0837898533113786 >1k 0.0012578592871489017 1.0297502832617509 >5k 1.2578592871489018E-4 0.41627987894592494 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6575 0.2212847303439421 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5781 0.19456228534119077 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3854 0.12970819022746052 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 3.365547229565659E-5 0.0 0.0 0.0 0.0 14-15 3.365547229565659E-5 0.0 0.0 0.0 3.365547229565659E-5 16-17 5.048320844348489E-5 0.0 0.0 0.0 6.731094459131319E-5 18-19 6.731094459131319E-5 0.0 0.0 3.365547229565659E-5 6.731094459131319E-5 20-21 6.731094459131319E-5 0.0 0.0 5.048320844348489E-5 1.0096641688696978E-4 22-23 1.0096641688696978E-4 0.0 0.0 8.413868073914148E-5 1.514496253304547E-4 24-25 1.0096641688696978E-4 0.0 0.0 3.533824591043942E-4 1.6827736147828298E-4 26-27 1.0096641688696978E-4 0.0 0.0 6.562817097653035E-4 1.6827736147828298E-4 28-29 1.5144962533045466E-4 0.0 0.0 0.002608299102913386 1.6827736147828298E-4 30-31 2.0193283377393956E-4 3.365547229565659E-5 0.0 0.007841725044887986 1.6827736147828298E-4 32-33 2.0193283377393956E-4 3.365547229565659E-5 0.0 0.020782254142567947 1.6827736147828298E-4 34-35 2.3558830606959616E-4 3.365547229565659E-5 0.0 0.04249003377326645 1.6827736147828298E-4 36-37 2.3558830606959616E-4 3.365547229565659E-5 0.0 0.07956153650693218 1.6827736147828298E-4 38-39 2.6924377836525274E-4 3.365547229565659E-5 0.0 0.1344031286127046 1.8510509762611127E-4 40-41 2.6924377836525274E-4 3.365547229565659E-5 0.0 0.19330020513010365 2.0193283377393956E-4 42-43 2.6924377836525274E-4 5.048320844348489E-5 0.0 0.2565388375736424 2.1876056992176785E-4 44-45 3.7021019525222253E-4 6.731094459131319E-5 0.0 0.3339800793259482 2.524160422174244E-4 46-47 4.206934036957074E-4 6.731094459131319E-5 0.0 0.43026838556382174 2.6924377836525274E-4 48-49 4.375211398435357E-4 6.731094459131319E-5 0.0 0.5362326400866965 2.6924377836525274E-4 50-51 4.375211398435357E-4 6.731094459131319E-5 0.0 0.647127421300885 3.028992506609093E-4 52-53 4.375211398435357E-4 6.731094459131319E-5 0.0 0.7561711515388123 3.3655472295656595E-4 54-55 4.375211398435357E-4 6.731094459131319E-5 0.0 0.8785761042781153 3.3655472295656595E-4 56-57 4.375211398435357E-4 6.731094459131319E-5 0.0 1.0217296556876907 3.3655472295656595E-4 58-59 4.375211398435357E-4 6.731094459131319E-5 0.0 1.1749630210498152 3.3655472295656595E-4 60-61 4.375211398435357E-4 6.731094459131319E-5 0.0 1.3443678408500026 3.7021019525222253E-4 62-63 4.375211398435357E-4 1.0096641688696978E-4 0.0 1.5147486693467642 3.7021019525222253E-4 64-65 4.375211398435357E-4 1.0096641688696978E-4 0.0 1.6974642284398838 3.7021019525222253E-4 66-67 4.711766121391923E-4 1.0096641688696978E-4 0.0 1.905202631184824 4.206934036957074E-4 68-69 4.711766121391923E-4 1.0096641688696978E-4 0.0 2.135254612061785 4.375211398435357E-4 70-71 5.216598205826772E-4 1.0096641688696978E-4 0.0 2.3840022077989826 4.375211398435357E-4 72-73 5.384875567305055E-4 1.0096641688696978E-4 0.0 2.64220699125126 4.54348875991364E-4 74-75 5.553152928783338E-4 1.0096641688696978E-4 0.0 2.904080221183764 4.880043482870206E-4 76-77 5.721430290261621E-4 1.0096641688696978E-4 0.0 3.193668732551741 5.048320844348489E-4 78-79 5.721430290261621E-4 1.1779415303479808E-4 0.0 3.521489860447584 5.384875567305055E-4 80-81 6.562817097653036E-4 1.3462188918262637E-4 0.0 3.8716245664754476 5.553152928783338E-4 82-83 7.067649182087884E-4 1.3462188918262637E-4 0.0 4.229012026783025 6.394539736174753E-4 84-85 7.909035989479299E-4 1.3462188918262637E-4 0.0 4.583420977792437 6.394539736174753E-4 86-87 9.08697751982728E-4 1.3462188918262637E-4 0.0 4.953412412474737 6.394539736174753E-4 88-89 9.08697751982728E-4 1.3462188918262637E-4 0.0 5.362023501616305 6.394539736174753E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4790 0.0 46.941425 1 GTATCAA 7800 0.0 39.248398 1 ATCAACG 9840 0.0 30.656603 3 TCAACGC 10110 0.0 29.88487 4 TATCAAC 10105 0.0 29.872257 2 CAACGCA 10350 0.0 29.329586 5 AACGCAG 10840 0.0 27.990606 6 ACGCAGA 12295 0.0 24.485075 7 CGCAGAG 12450 0.0 24.103962 8 GTACATG 9405 0.0 23.654715 1 TACATGG 9500 0.0 22.717625 2 TGGTATC 2520 0.0 21.504778 2 ACATGGG 9745 0.0 21.351957 3 GCAGAGT 13800 0.0 21.333067 9 GTGGTAT 2600 0.0 20.843094 1 GAGTACT 7935 0.0 20.106342 12-13 GTACTTT 8020 0.0 19.804436 14-15 AGAGTAC 12555 0.0 19.30722 10-11 CAGAGTA 13205 0.0 19.093996 10-11 AGTACTT 8375 0.0 18.483044 12-13 >>END_MODULE