FastQCFastQC Report
Thu 26 May 2016
SRR938534_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938534_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942050
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65350.22212402916333848No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52360.1779711425706565No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35600.1210040617936473No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45600.048.2532041
GTATCAA75500.040.813741
ATCAACG94650.032.4366843
TATCAAC95950.031.9972132
TCAACGC96000.031.9310424
CAACGCA96750.031.5340025
AACGCAG102850.029.7520926
ACGCAGA118000.025.7672147
CGCAGAG119900.025.0423328
GTACATG95850.024.6455541
TACATGG95700.023.9364472
ACATGGG99950.022.2054043
GCAGAGT130150.022.0111459
GAGTACT70800.020.99974812-13
GTACTTT75450.019.61292814-15
CATGGGG69900.019.1732834
CAGAGTA126950.019.06564110-11
AGAGTAC118450.018.98999410-11
AGTACTT73400.018.63800612-13
ATTCGCG4200.018.08963280-81
Produced by FastQC (version 0.11.5)