##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938534_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2942050 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.887720127122243 31.0 31.0 34.0 27.0 34.0 2 30.985440084294964 31.0 31.0 34.0 27.0 34.0 3 31.05198280110807 31.0 31.0 34.0 27.0 34.0 4 34.67415305654221 37.0 35.0 37.0 32.0 37.0 5 34.67870362502337 37.0 35.0 37.0 32.0 37.0 6 34.70420183205588 37.0 35.0 37.0 31.0 37.0 7 34.61544909161979 37.0 35.0 37.0 30.0 37.0 8 34.568201084277966 37.0 35.0 37.0 30.0 37.0 9 36.0533308407403 39.0 35.0 39.0 30.0 39.0 10-11 35.8886242585952 38.0 35.0 39.0 30.0 39.0 12-13 35.71840859264799 38.0 35.0 39.0 30.0 39.0 14-15 36.682728029775156 39.0 36.0 41.0 30.0 41.0 16-17 36.50919970768682 39.0 36.0 40.5 30.0 41.0 18-19 36.47562515932768 39.0 36.0 40.5 29.5 41.0 20-21 36.56110178277052 39.0 36.0 41.0 30.0 41.0 22-23 36.47138168963818 39.0 36.0 41.0 29.0 41.0 24-25 36.3477612549073 39.0 35.5 41.0 29.0 41.0 26-27 36.144724936693805 39.0 35.0 41.0 27.5 41.0 28-29 35.94729576315834 39.0 35.0 40.0 27.0 41.0 30-31 35.729965840145475 38.5 35.0 40.0 27.0 41.0 32-33 35.44976988834316 38.0 34.0 40.0 26.5 41.0 34-35 35.192727010078 38.0 34.0 40.0 25.5 41.0 36-37 34.89611172481773 38.0 34.0 40.0 25.0 41.0 38-39 34.62880338539454 38.0 33.0 40.0 24.0 41.0 40-41 34.563474278139395 38.0 33.0 40.0 24.0 41.0 42-43 34.38542393909009 38.0 33.0 40.0 24.0 41.0 44-45 34.100829523631475 38.0 33.0 40.0 23.0 41.0 46-47 33.73634574531364 38.0 33.0 40.0 21.5 41.0 48-49 33.3695484441121 38.0 32.0 40.0 18.5 41.0 50-51 32.62529188830917 36.5 30.5 39.5 17.5 40.5 52-53 32.68708009721113 37.0 31.0 39.0 17.5 40.0 54-55 32.79071089886304 37.0 31.0 40.0 16.0 41.0 56-57 32.46436566339797 37.0 31.0 40.0 12.5 41.0 58-59 32.100082595469146 36.0 31.0 39.0 8.5 41.0 60-61 31.618747641950343 36.0 30.0 39.0 7.5 40.5 62-63 31.06552709845176 35.0 29.0 39.0 4.5 40.0 64-65 30.534092384561788 35.0 29.0 38.0 2.0 40.0 66-67 29.96132798558828 34.5 28.0 38.0 2.0 40.0 68-69 29.37433711187777 34.0 27.5 37.5 2.0 39.5 70-71 28.73889158919801 34.0 26.0 36.5 2.0 39.0 72-73 28.15602148161996 33.5 26.0 36.0 2.0 39.0 74-75 27.50417735932428 33.0 25.5 35.5 2.0 38.5 76-77 26.74368977413708 32.5 23.5 35.0 2.0 37.5 78-79 25.949167247327544 32.0 21.0 35.0 2.0 37.0 80-81 25.271607042708318 31.0 19.0 35.0 2.0 36.0 82-83 24.597943780697133 31.0 16.5 34.0 2.0 36.0 84-85 23.92704066892133 31.0 9.0 34.0 2.0 35.0 86-87 23.23077361023776 30.0 4.5 34.0 2.0 35.0 88-89 22.42675362417362 29.5 2.0 34.0 2.0 35.0 90-91 21.576509066807155 29.0 2.0 34.0 2.0 35.0 92-93 20.703163440458184 28.0 2.0 34.0 2.0 35.0 94-95 19.72904964225625 26.5 2.0 33.5 2.0 35.0 96-97 18.48672405295627 25.0 2.0 33.0 2.0 34.5 98-99 17.008214000441868 20.0 2.0 32.0 2.0 34.0 100-101 13.720798422868409 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5956960627891377 1101 2 0.6379905081630035 1101 3 0.5783726191106524 1101 4 0.6147532733897236 1101 5 0.5121808985066636 1101 6 0.5215194051638221 1101 7 0.42212717963243307 1101 8 0.4962163831652191 1101 9 0.5913236026945867 1101 10-11 0.5385565747061847 1101 12-13 0.5985732803666863 1101 14-15 0.728264583342721 1101 16-17 0.7107634728114434 1101 18-19 0.5195484978344638 1101 20-21 0.6925519984467101 1101 22-23 0.8101864295520897 1101 24-25 0.7791264550062067 1101 26-27 0.836472487560016 1101 28-29 0.8179955816874553 1101 30-31 0.7196986182311562 1101 32-33 0.7552819316822905 1101 34-35 0.7566003911995836 1101 36-37 0.7896654507134997 1101 38-39 0.8497091623584723 1101 40-41 0.8808779961425515 1101 42-43 0.8725881418374968 1101 44-45 0.7772181496372212 1101 46-47 0.944923980164333 1101 48-49 1.05696922688103 1101 50-51 0.913758798206068 1101 52-53 1.0073971906805994 1101 54-55 1.0233852618232362 1101 56-57 0.9922481163821502 1101 58-59 1.166246395310516 1101 60-61 1.0417605368402008 1101 62-63 0.8873506750225246 1101 64-65 0.821326202268704 1101 66-67 0.8183182479641609 1101 68-69 0.6898511779784968 1101 70-71 1.0169655650697074 1101 72-73 0.8523367303244989 1101 74-75 0.6502490339620408 1101 76-77 0.9151311450996289 1101 78-79 0.7074759478756221 1101 80-81 0.7009051335407612 1101 82-83 0.9048186289398679 1101 84-85 0.9987301387270549 1101 86-87 1.0874349713490723 1101 88-89 1.0897420380509573 1101 90-91 0.951131017451246 1101 92-93 0.9350180818871969 1101 94-95 0.7971633783908239 1101 96-97 0.9921392912563505 1101 98-99 1.011947556110524 1101 100-101 0.9138754949165477 1102 1 0.5303239047975907 1102 2 0.6331302695111596 1102 3 0.546220087609349 1102 4 0.458819578526942 1102 5 0.47470773491193796 1102 6 0.5777072838553181 1102 7 0.410370846240788 1102 8 0.5106592726442898 1102 9 0.4604552428826665 1102 10-11 0.7012001660400884 1102 12-13 0.733378839014506 1102 14-15 0.6496993834678335 1102 16-17 0.5879380830540626 1102 18-19 0.7239360181771275 1102 20-21 0.7339601079445401 1102 22-23 0.6623203544790712 1102 24-25 0.509043812130507 1102 26-27 0.4407136997968877 1102 28-29 0.4521679336962805 1102 30-31 0.48941893650429336 1102 32-33 0.4285389568568263 1102 34-35 0.5465474075214161 1102 36-37 0.3625531274362004 1102 38-39 0.5401414869080199 1102 40-41 0.656785530479894 1102 42-43 0.8702423087029558 1102 44-45 0.7515321978658847 1102 46-47 0.5957941423224824 1102 48-49 0.4644553249604755 1102 50-51 0.570798285491577 1102 52-53 0.6702477867801164 1102 54-55 0.6266786519834895 1102 56-57 0.6239580128532722 1102 58-59 0.6509075012089554 1102 60-61 0.6714238426612482 1102 62-63 0.38185192871335616 1102 64-65 0.44200123394228896 1102 66-67 0.507907313023555 1102 68-69 0.25535056040986603 1102 70-71 0.49260208484515644 1102 72-73 0.7866475714370758 1102 74-75 0.5929215688559957 1102 76-77 0.6010953738279667 1102 78-79 0.609964510928588 1102 80-81 0.6715157578416893 1102 82-83 0.8044400037059596 1102 84-85 0.8294533831623099 1102 86-87 0.652931835796732 1102 88-89 0.8101734879324809 1102 90-91 0.9297321317252987 1102 92-93 1.0996139057449597 1102 94-95 1.1246094007717211 1102 96-97 1.1285918762979392 1102 98-99 1.4559193705960887 1102 100-101 1.2199609314289024 1103 1 0.6867166629840504 1103 2 0.6790766391573904 1103 3 0.6641541159013222 1103 4 0.6646507764982417 1103 5 0.7436155189321099 1103 6 0.7097541062425563 1103 7 0.5605987997554323 1103 8 0.5543102770389297 1103 9 0.6476263213826314 1103 10-11 0.6519265380761468 1103 12-13 0.649620092881392 1103 14-15 0.8532486207120797 1103 16-17 0.814702041887621 1103 18-19 0.9847029152824689 1103 20-21 0.8893846764720479 1103 22-23 0.8743444427926761 1103 24-25 1.0302589616432982 1103 26-27 1.0583866590154756 1103 28-29 0.9231540318000313 1103 30-31 0.952623644275441 1103 32-33 1.0985171368441016 1103 34-35 0.9954231891966927 1103 36-37 1.1559322114756725 1103 38-39 1.0635714468629658 1103 40-41 1.2386375149112467 1103 42-43 0.9570943084537546 1103 44-45 1.2485475847281222 1103 46-47 1.262190505687741 1103 48-49 1.2429288330241235 1103 50-51 1.3592623771958898 1103 52-53 1.3078391035078383 1103 54-55 1.2865687144562301 1103 56-57 1.3552332963398115 1103 58-59 1.2928367774054834 1103 60-61 1.2225432098247069 1103 62-63 1.375523205855611 1103 64-65 1.140629223406588 1103 66-67 1.2590330377615189 1103 68-69 1.3196945761705194 1103 70-71 1.5686340800409724 1103 72-73 1.4859792346092071 1103 74-75 1.3153127164569192 1103 76-77 1.2553334504395508 1103 78-79 1.363997102561914 1103 80-81 1.358745305601115 1103 82-83 1.5241216500777526 1103 84-85 1.5943378948852676 1103 86-87 1.6212623060938363 1103 88-89 1.8295646008093023 1103 90-91 1.6019442714755066 1103 92-93 1.6710600215988585 1103 94-95 1.6417327234188868 1103 96-97 1.6764673485201875 1103 98-99 1.6475526238256855 1103 100-101 1.3133949357203925 1104 1 0.49197268552005724 1104 2 0.4632662481201848 1104 3 0.5189441897376241 1104 4 0.5893121401923622 1104 5 0.45866350177597326 1104 6 0.48732029402565047 1104 7 0.5094038226569992 1104 8 0.3973018397215924 1104 9 0.47915781372824284 1104 10-11 0.5113181852101789 1104 12-13 0.46400228810871624 1104 14-15 0.5832592308927786 1104 16-17 0.6560103196857128 1104 18-19 0.7047416172727452 1104 20-21 0.6302090205566415 1104 22-23 0.7059326270295898 1104 24-25 0.7176822321007563 1104 26-27 0.709358142994148 1104 28-29 0.6578468546065963 1104 30-31 0.8272134833291176 1104 32-33 0.6167515216491068 1104 34-35 0.6620951503646992 1104 36-37 0.881118146217446 1104 38-39 0.8270808557620271 1104 40-41 0.8843928561671106 1104 42-43 0.7627986123457546 1104 44-45 0.807206709092533 1104 46-47 0.842021607836756 1104 48-49 0.8645000732276671 1104 50-51 0.5224746668249836 1104 52-53 0.7252032189090656 1104 54-55 0.767116553615466 1104 56-57 0.8613137460433578 1104 58-59 0.6911193063330145 1104 60-61 0.7448170140656529 1104 62-63 0.7299919441873399 1104 64-65 0.7425950880458991 1104 66-67 0.9231111005409467 1104 68-69 0.9151176589582768 1104 70-71 0.7664785174912438 1104 72-73 0.8495845310002785 1104 74-75 0.7826366832471976 1104 76-77 0.926148440362077 1104 78-79 0.8923849562264614 1104 80-81 0.8590575997264658 1104 82-83 0.6822083329569928 1104 84-85 0.3886275796979035 1104 86-87 0.4984691357073352 1104 88-89 0.6932133935652516 1104 90-91 0.6397395580334724 1104 92-93 0.7831927949857338 1104 94-95 0.7642137218608376 1104 96-97 0.5902173558510349 1104 98-99 0.42567595605776276 1104 100-101 0.3960551342984022 1105 1 0.5139899507395782 1105 2 0.5784445300775118 1105 3 0.4389837874243874 1105 4 0.5646954397040176 1105 5 0.5208416608853312 1105 6 0.5879100335417746 1105 7 0.5889080479804889 1105 8 0.5924300172581454 1105 9 0.7124157371911934 1105 10-11 0.6596470603015092 1105 12-13 0.6576870020345993 1105 14-15 0.7741182683928116 1105 16-17 0.7422039285433257 1105 18-19 0.8788240561096927 1105 20-21 0.886588166790979 1105 22-23 0.8976293745142101 1105 24-25 0.8731012475805286 1105 26-27 0.9941672991394626 1105 28-29 1.0469472957508756 1105 30-31 0.9884290985988429 1105 32-33 1.0094291016180605 1105 34-35 1.0577620116176192 1105 36-37 1.0800186453681277 1105 38-39 1.198928315995765 1105 40-41 1.1183803626212452 1105 42-43 1.110322636974466 1105 44-45 1.0797119601503695 1105 46-47 1.1183531574114554 1105 48-49 1.0933581575013704 1105 50-51 1.0441784619034848 1105 52-53 1.0299245410672384 1105 54-55 1.0200487406140937 1105 56-57 1.2590960400490232 1105 58-59 1.2429904748388552 1105 60-61 1.3320606613017105 1105 62-63 1.331264275038162 1105 64-65 1.390504328799281 1105 66-67 1.3000033517899787 1105 68-69 1.1331787462643845 1105 70-71 1.222400343695547 1105 72-73 1.2198176488724393 1105 74-75 1.2134035408978114 1105 76-77 1.2937954007062018 1105 78-79 1.2886224716863701 1105 80-81 1.430354449368867 1105 82-83 1.3878167467495892 1105 84-85 1.3536182760426705 1105 86-87 1.333972706149563 1105 88-89 1.4094889133830293 1105 90-91 1.4833288685053851 1105 92-93 1.3805403988499911 1105 94-95 1.358265675004958 1105 96-97 1.3713851204521603 1105 98-99 1.308302690361625 1105 100-101 0.9917830269754635 1106 1 0.3193571300625422 1106 2 0.3691599261165628 1106 3 0.14829726128198928 1106 4 0.44328436051112163 1106 5 0.41602947283981706 1106 6 0.17993059749571927 1106 7 0.25956727257913315 1106 8 0.38285852223026495 1106 9 0.476385970576338 1106 10-11 0.29086249199007597 1106 12-13 0.3133027506027659 1106 14-15 0.5425640137214458 1106 16-17 0.3043144886091511 1106 18-19 0.08419647834148236 1106 20-21 0.3654385098794819 1106 22-23 0.36370662448197777 1106 24-25 0.38644439404220066 1106 26-27 0.0938590070610772 1106 28-29 0.12383783048124286 1106 30-31 0.2838447510997497 1106 32-33 0.41496872066422696 1106 34-35 0.39576951869469923 1106 36-37 0.2977982870401661 1106 38-39 0.2530663778302298 1106 40-41 0.38704701068161285 1106 42-43 0.09195607199837497 1106 44-45 0.28485155881248403 1106 46-47 0.2230613347334085 1106 48-49 0.6042058375537636 1106 50-51 0.258344168782628 1106 52-53 0.48969939655108874 1106 54-55 0.270765488058089 1106 56-57 -0.21063226315020245 1106 58-59 -0.5403203859258667 1106 60-61 0.21798247936170156 1106 62-63 0.2641583753985053 1106 64-65 0.37539181360303786 1106 66-67 -0.3547901414437895 1106 68-69 -0.3675829444336003 1106 70-71 -0.3409518479137894 1106 72-73 0.1792017155595289 1106 74-75 0.31351733687792205 1106 76-77 0.22512210861482274 1106 78-79 0.462765518803959 1106 80-81 0.39075280422543557 1106 82-83 0.4671187370549532 1106 84-85 0.3133773124050663 1106 86-87 0.5267662714733241 1106 88-89 0.4751271375329722 1106 90-91 0.27976457185572556 1106 92-93 0.13423142361154916 1106 94-95 0.006378979236380644 1106 96-97 0.13285672360373724 1106 98-99 0.07663668489657027 1106 100-101 -0.1319120508709961 1107 1 0.2734184922199958 1107 2 0.2952543528983753 1107 3 0.18994904572124582 1107 4 0.28384219509823794 1107 5 0.2904632084518468 1107 6 0.08460233590050592 1107 7 0.07520986004996644 1107 8 0.16820400071092223 1107 9 0.17312706878051642 1107 10-11 0.23321768428757395 1107 12-13 0.14219973743278302 1107 14-15 0.26152490692194874 1107 16-17 0.23399851415582162 1107 18-19 0.17614482481803861 1107 20-21 0.2606358887401683 1107 22-23 0.34553483209867863 1107 24-25 0.33559178163098125 1107 26-27 0.2711558755463699 1107 28-29 0.3730612070264314 1107 30-31 0.23831318513729371 1107 32-33 0.18001221279070023 1107 34-35 0.3054973181147318 1107 36-37 0.3023691479274504 1107 38-39 0.357870818470019 1107 40-41 0.2846144267582531 1107 42-43 0.2373210930169165 1107 44-45 0.3409648433383552 1107 46-47 0.3459907569714318 1107 48-49 0.36419927853021505 1107 50-51 0.35157201005117145 1107 52-53 0.2988333551235982 1107 54-55 0.2978919244848015 1107 56-57 0.5205767353713711 1107 58-59 0.5507904389661817 1107 60-61 0.33964492276558644 1107 62-63 0.43604234022899746 1107 64-65 0.3712314981876652 1107 66-67 0.4886278927612757 1107 68-69 0.4037092637044637 1107 70-71 0.48207107459434795 1107 72-73 0.4674547558069868 1107 74-75 0.5615551158831558 1107 76-77 0.5653030309207168 1107 78-79 0.6118861542220486 1107 80-81 0.42420525786594254 1107 82-83 0.39943342947722016 1107 84-85 0.42562025696113537 1107 86-87 0.26128246023072776 1107 88-89 0.6497760814563023 1107 90-91 0.7004968755870351 1107 92-93 0.7711228654862872 1107 94-95 0.9996849266982508 1107 96-97 0.9717783310320627 1107 98-99 0.7931765397313093 1107 100-101 0.3654595979812907 1108 1 0.3112027782958542 1108 2 0.349019388579638 1108 3 0.3355379028065464 1108 4 0.4233027737759585 1108 5 0.37254819560865826 1108 6 0.2689067117348003 1108 7 0.3561432769138335 1108 8 0.2400328841245809 1108 9 0.23918186687938459 1108 10-11 0.38249642170726617 1108 12-13 0.3686014381499447 1108 14-15 0.4868913307449816 1108 16-17 0.4748099705314033 1108 18-19 0.3768483408485963 1108 20-21 0.441174011354434 1108 22-23 0.565816740284447 1108 24-25 0.4803449643879816 1108 26-27 0.6102134857908155 1108 28-29 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-0.5477220723255556 2316 82-83 -0.5785200422082681 2316 84-85 -0.7350672975147567 2316 86-87 -0.6310023001436669 2316 88-89 -0.7040471561456307 2316 90-91 -0.8539093146182317 2316 92-93 -0.9076353630696019 2316 94-95 -1.0263565259188034 2316 96-97 -0.9132939154617219 2316 98-99 -0.8728049472098256 2316 100-101 -0.8229782707683047 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11846.0 3 4565.0 4 4993.0 5 5442.0 6 6346.0 7 7843.0 8 9781.0 9 12160.0 10 14910.0 11 17847.0 12 20976.0 13 23767.0 14 26645.0 15 30179.0 16 33581.0 17 37866.0 18 37918.0 19 37155.0 20 39824.0 21 42784.0 22 47654.0 23 52824.0 24 57851.0 25 65175.0 26 72515.0 27 81350.0 28 92571.0 29 105328.0 30 120810.0 31 140059.0 32 162978.0 33 194602.0 34 234609.0 35 284517.0 36 333452.0 37 319898.0 38 142265.0 39 7164.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.86445932260988 17.994805127586964 14.04999145311369 24.09074409668946 2 15.902226363379773 19.215824965058985 39.85785552272102 25.024093148840233 3 19.70589975577227 22.860796994455058 28.73399444621651 28.69930880355616 4 13.356079714583133 15.644536158482403 35.59351833557158 35.405865791362885 5 14.6525680272051 36.422634050384126 33.57795431874847 15.346843603662307 6 34.03193001789006 34.53791944710868 16.87753810991164 14.55261242508962 7 30.305529950313176 31.235853127196307 19.70485759877267 18.75375932371785 8 26.968253142503336 34.87669527212588 19.00530661359713 19.149744971773657 9 26.642841233867255 16.60235953394189 19.4292097312498 37.32558950094106 10-11 25.77976285606444 25.816402172175884 26.720466670973842 21.683368300785833 12-13 26.389990433351983 22.89968552641131 27.164860020404998 23.545464019831712 14-15 23.16448418210126 24.631434782002586 25.10668747090767 27.097393564988487 16-17 23.24295065423967 27.7494080494396 25.78879707213977 23.21884422418096 18-19 23.2656033388498 26.47303047067676 27.610253076191484 22.651113114281955 20-21 23.623836753571144 25.765655473315686 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23.267590332962772 46-47 23.379996478253215 26.36494882929114 26.912060351046087 23.342994341409558 48-49 23.39277413148249 26.20332135339074 27.016977298164598 23.38692721696217 50-51 23.406387096905778 26.434713913833885 26.901332038890395 23.25756695036994 52-53 23.549099687927622 26.326562558690974 26.79748404497881 23.326853708402602 54-55 23.17957978149943 26.312768517453073 27.017625503891164 23.490026197156336 56-57 23.215461496336783 26.514857684603122 26.992794574498497 23.2768862445616 58-59 23.371701169731487 26.14512448145122 26.96304079536506 23.52013355345223 60-61 23.203497932769324 26.281933721142693 27.172971755637608 23.341596590450372 62-63 23.159481050272234 26.404208512608342 27.16314597636527 23.273164460754156 64-65 22.97469164433523 26.237752410936704 27.14714476871647 23.640411176011593 66-67 22.97698255098163 26.31768403319984 27.025073913761727 23.680259502056806 68-69 23.069632925170254 26.10787688345699 27.19943721492774 23.623052976445017 70-71 23.02635705615143 26.118104864886366 27.02423969311155 23.83129838585066 72-73 23.130999864863604 26.227476204520016 26.864980039758997 23.776543890857386 74-75 23.039480993491 26.489682355668908 26.83576828696983 23.63506836387026 76-77 23.1810088919899 26.211322223515882 26.84857441761843 23.759094466875787 78-79 23.262939902291617 26.249138529547484 26.879050823145107 23.608870745015793 80-81 23.214043217194284 26.470246814814363 26.69530839572155 23.620401572269802 82-83 23.301780568493633 26.39848941032106 26.65740393742474 23.642326083760565 84-85 23.296197047395886 26.304139332539407 26.678674732397628 23.720988887667083 86-87 23.27305533252612 26.209678790025475 26.709642417909496 23.80762345953891 88-89 23.273007554407272 26.27693758697564 26.594716368879745 23.855338489737342 90-91 23.281493805336215 26.299957939462065 26.598988982995238 23.819559272206483 92-93 23.3616078030737 25.930805176437794 26.73166394971111 23.975923070777398 94-95 23.26244640895136 25.911521707827866 26.780572631797984 24.04545925142279 96-97 23.389951127774168 25.80056384883357 26.664442960840507 24.14504206255176 98-99 23.328644026539347 25.83345118927982 26.42520136368396 24.41270342049687 100-101 23.928041211677776 25.259593583433652 26.575151332105378 24.237213872783194 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 436.0 1 310.0 2 257.0 3 373.0 4 315.5 5 287.5 6 537.0 7 1106.0 8 1802.0 9 2110.5 10 1903.0 11 1699.0 12 1778.5 13 1923.0 14 2026.5 15 2187.0 16 2397.5 17 2536.0 18 2721.5 19 2993.0 20 3338.5 21 3889.5 22 4756.0 23 5920.5 24 7202.5 25 8979.5 26 11472.0 27 14649.0 28 17707.0 29 20910.5 30 24995.0 31 29909.0 32 35985.5 33 40367.5 34 43330.5 35 47528.5 36 53422.0 37 59946.5 38 65406.5 39 70083.5 40 74966.0 41 81987.0 42 88592.5 43 93716.0 44 100015.5 45 106635.5 46 110356.5 47 113350.5 48 116224.0 49 116518.0 50 115722.0 51 113604.5 52 112195.0 53 112350.5 54 113044.0 55 109291.5 56 100621.0 57 92661.5 58 85371.5 59 76003.5 60 63752.5 61 53113.5 62 44376.0 63 35066.0 64 27414.5 65 21573.5 66 16292.0 67 12397.5 68 9389.5 69 6821.5 70 5081.5 71 3769.0 72 2838.0 73 2242.0 74 1693.5 75 1277.0 76 935.5 77 579.0 78 342.5 79 218.0 80 142.5 81 79.0 82 45.0 83 30.0 84 17.0 85 11.5 86 8.5 87 6.0 88 5.0 89 4.0 90 2.5 91 3.5 92 3.5 93 2.0 94 2.0 95 2.5 96 2.5 97 1.0 98 0.0 99 0.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18041841573052803 2 0.04690606889753743 3 0.0459543515575874 4 0.05129076664230724 5 0.0584286466919325 6 0.06356112234666304 7 0.03497561224316378 8 0.03409187471321017 9 0.04129773457283187 10-11 0.04146768409782295 12-13 0.037507860165530836 14-15 0.03341207661324587 16-17 0.03147465202834758 18-19 0.039326320082935365 20-21 0.04979521082238575 22-23 0.046787104230043675 24-25 0.06716405227647389 26-27 0.051919579884774227 28-29 0.043846977447698034 30-31 0.053517105419690354 32-33 0.04920038748491697 34-35 0.04891147329243215 36-37 0.04615829098757669 38-39 0.025424448938665217 40-41 0.023826923403749086 42-43 0.020767831953909688 44-45 0.003891844122295678 46-47 0.011148688839414694 48-49 0.011131693886915586 50-51 0.008293536819564589 52-53 0.008990329872028007 54-55 0.010825784741931646 56-57 0.008123587294573511 58-59 0.01041790588195306 60-61 0.010621845311942352 62-63 0.003670909739807277 64-65 0.006645026427151136 66-67 0.003670909739807277 68-69 0.008565456059550313 70-71 0.02228038272633028 72-73 0.01962917013646947 74-75 0.01985010451895787 76-77 0.012151391036862053 78-79 0.004503662412263558 80-81 0.006407097092163628 82-83 0.005183460512227869 84-85 0.004707601842252851 86-87 0.008021617579578864 88-89 0.028993388963477848 90-91 0.03572339015312452 92-93 0.021923488723849017 94-95 0.030947808500875237 96-97 0.03444876871569144 98-99 0.02059788242891861 100-101 0.12108903655614282 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2942050.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.93945681361189 #Duplication Level Percentage of deduplicated Percentage of total 1 78.38869633140433 44.63409789437334 2 12.097879734822088 13.776934013943453 3 3.9157838018831326 6.688878080362967 4 1.800789784205692 4.101439685926938 5 0.9540735264876471 2.7162214179226893 6 0.6133744120893856 2.095512350864289 7 0.40779351958323856 1.6253679048026424 8 0.28139426000023593 1.2817949051905366 9 0.22375639345631446 1.1466510751777828 >10 1.1905103766295353 12.241824606881565 >50 0.07886509231473746 3.1191686257751474 >100 0.04335732214811874 4.304324116449595 >500 0.0024636007098217255 0.9511402918941486 >1k 0.0011416686213428643 0.913915808356218 >5k 1.2017564435188044E-4 0.4027292220785056 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6535 0.22212402916333848 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5236 0.1779711425706565 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3560 0.1210040617936473 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 3.398990499821553E-5 3 0.0 0.0 0.0 0.0 3.398990499821553E-5 4 0.0 0.0 0.0 0.0 3.398990499821553E-5 5 0.0 0.0 0.0 0.0 3.398990499821553E-5 6 0.0 0.0 0.0 0.0 1.0196971499464659E-4 7 0.0 0.0 0.0 0.0 1.0196971499464659E-4 8 0.0 0.0 0.0 0.0 1.0196971499464659E-4 9 0.0 0.0 0.0 3.398990499821553E-5 1.0196971499464659E-4 10-11 0.0 0.0 0.0 3.398990499821553E-5 1.0196971499464659E-4 12-13 0.0 0.0 0.0 3.398990499821553E-5 1.0196971499464659E-4 14-15 1.0196971499464659E-4 0.0 0.0 3.398990499821553E-5 1.8694447749018542E-4 16-17 1.1896466749375436E-4 0.0 0.0 3.398990499821553E-5 2.0393942998929318E-4 18-19 1.5295457249196989E-4 0.0 0.0 3.398990499821553E-5 2.0393942998929318E-4 20-21 2.0393942998929318E-4 0.0 0.0 1.0196971499464659E-4 2.2093438248840095E-4 22-23 2.0393942998929318E-4 0.0 0.0 1.5295457249196989E-4 2.379293349875087E-4 24-25 2.7191923998572424E-4 0.0 0.0 1.6994952499107765E-4 2.379293349875087E-4 26-27 2.7191923998572424E-4 0.0 0.0 5.778283849696641E-4 2.379293349875087E-4 28-29 3.568940024812631E-4 0.0 0.0 0.0022603286823813327 2.379293349875087E-4 30-31 3.7388895498037083E-4 0.0 0.0 0.00866742577454496 2.5492428748661645E-4 32-33 3.7388895498037083E-4 0.0 0.0 0.02168555938886151 2.7191923998572424E-4 34-35 4.0787885997858636E-4 0.0 0.0 0.04257235601026495 2.7191923998572424E-4 36-37 4.418687649768019E-4 0.0 0.0 0.08030115055828418 2.7191923998572424E-4 38-39 4.758586699750174E-4 0.0 0.0 0.13716626162029877 2.7191923998572424E-4 40-41 4.758586699750174E-4 0.0 0.0 0.199180843289543 2.7191923998572424E-4 42-43 4.928536224741251E-4 3.398990499821553E-5 0.0 0.2630308798286909 2.7191923998572424E-4 44-45 5.098485749732329E-4 3.398990499821553E-5 0.0 0.3426522322870108 2.7191923998572424E-4 46-47 5.438384799714485E-4 3.398990499821553E-5 0.0 0.43809588552199996 2.7191923998572424E-4 48-49 5.438384799714485E-4 3.398990499821553E-5 0.0 0.5440934042589352 3.0590914498393977E-4 50-51 6.118182899678795E-4 3.398990499821553E-5 0.0 0.6541697115956561 3.0590914498393977E-4 52-53 6.118182899678795E-4 3.398990499821553E-5 0.0 0.7620706650124913 3.0590914498393977E-4 54-55 6.45808194966095E-4 3.398990499821553E-5 0.0 0.885963868730987 3.229040974830475E-4 56-57 6.967930524634184E-4 3.398990499821553E-5 0.0 1.0347716728131746 3.398990499821553E-4 58-59 7.477779099607417E-4 3.398990499821553E-5 0.0 1.1971754388946483 3.398990499821553E-4 60-61 7.477779099607417E-4 3.398990499821553E-5 0.0 1.3698441562855832 3.7388895498037083E-4 62-63 7.647728624598493E-4 3.398990499821553E-5 0.0 1.5403375197566325 3.7388895498037083E-4 64-65 7.817678149589571E-4 6.797980999643106E-5 0.0 1.71866555632977 3.7388895498037083E-4 66-67 7.817678149589571E-4 8.497476249553883E-5 0.0 1.9313403919036047 3.9088390747947857E-4 68-69 7.817678149589571E-4 1.0196971499464659E-4 0.0 2.165275913053823 4.0787885997858636E-4 70-71 8.157577199571727E-4 1.0196971499464659E-4 0.0 2.4205231046379225 4.0787885997858636E-4 72-73 8.66742577454496E-4 1.0196971499464659E-4 0.0 2.6767220135619723 4.0787885997858636E-4 74-75 8.837375299536038E-4 1.0196971499464659E-4 0.0 2.9351132713584063 4.2487381247769416E-4 76-77 8.837375299536038E-4 1.0196971499464659E-4 0.0 3.2197617307659625 4.418687649768019E-4 78-79 8.837375299536038E-4 1.0196971499464659E-4 0.0 3.540473479376625 4.418687649768019E-4 80-81 0.0010027021974473582 1.0196971499464659E-4 0.0 3.876701619618973 4.5886371747590963E-4 82-83 0.0010196971499464658 1.0196971499464659E-4 0.0 4.228208222158019 5.438384799714485E-4 84-85 0.0010706820074437892 1.0196971499464659E-4 0.0 4.582451012049422 5.438384799714485E-4 86-87 0.0011386618174402201 1.0196971499464659E-4 0.0 4.951785319760031 5.608334324705563E-4 88-89 0.001155656769939328 1.0196971499464659E-4 0.0 5.358389558301184 6.118182899678795E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4560 0.0 48.253204 1 GTATCAA 7550 0.0 40.81374 1 ATCAACG 9465 0.0 32.436684 3 TATCAAC 9595 0.0 31.997213 2 TCAACGC 9600 0.0 31.931042 4 CAACGCA 9675 0.0 31.534002 5 AACGCAG 10285 0.0 29.752092 6 ACGCAGA 11800 0.0 25.767214 7 CGCAGAG 11990 0.0 25.042332 8 GTACATG 9585 0.0 24.645554 1 TACATGG 9570 0.0 23.936447 2 ACATGGG 9995 0.0 22.205404 3 GCAGAGT 13015 0.0 22.011145 9 GAGTACT 7080 0.0 20.999748 12-13 GTACTTT 7545 0.0 19.612928 14-15 CATGGGG 6990 0.0 19.173283 4 CAGAGTA 12695 0.0 19.065641 10-11 AGAGTAC 11845 0.0 18.989994 10-11 AGTACTT 7340 0.0 18.638006 12-13 ATTCGCG 420 0.0 18.089632 80-81 >>END_MODULE