FastQCFastQC Report
Thu 26 May 2016
SRR938534_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938534_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2942050
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68010.2311653438928638No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57670.19601978212470897No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39050.13273057901803165No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46950.047.3065031
GTATCAA73750.040.8852651
ATCAACG96850.030.8247873
TCAACGC97450.030.5374364
TATCAAC99700.030.0528012
CAACGCA99350.029.9534285
AACGCAG104350.028.6295346
ACGCAGA120450.024.5267317
CGCAGAG122300.024.000388
GTGGTAT25000.023.2091561
TACATGG90750.022.3779752
GTACATG93050.022.1315121
TGGTATC24900.021.3923382
GCAGAGT133450.021.3544679
GAGTACT75400.020.72433712-13
ACATGGG93950.020.5432573
GTACTTT79050.019.70734214-15
AGAGTAC122000.019.19301410-11
CAGAGTA129300.018.89917810-11
AGTACTT79450.018.6815212-13
Produced by FastQC (version 0.11.5)