FastQCFastQC Report
Thu 26 May 2016
SRR938533_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938533_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944499
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66270.2250637544791151No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54330.18451356240908895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36220.1230090416060593No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44500.044.567921
GTATCAA74150.041.92041
ATCAACG95450.032.363743
TATCAAC96450.032.0271032
TCAACGC97950.031.4891954
CAACGCA100550.030.627695
AACGCAG104600.029.5286716
ACGCAGA118600.025.6754177
CGCAGAG119350.025.3548588
GTACATG91650.024.604681
TACATGG92500.023.42772
GCAGAGT129850.022.6449179
ACATGGG95800.021.926943
GAGTACT72650.021.34625212-13
TGGTATC24950.020.7616792
AGTACTT76200.020.19594212-13
GTGGTAT27550.019.9005571
GTACTTT77600.019.80333314-15
AGAGTAC119450.019.78299510-11
CAGAGTA123900.019.32165710-11
Produced by FastQC (version 0.11.5)