##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938533_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944499 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.937899112888136 31.0 31.0 34.0 27.0 34.0 2 31.085291589503 31.0 31.0 34.0 27.0 34.0 3 31.13898900967533 31.0 31.0 34.0 27.0 34.0 4 34.79950001681101 37.0 35.0 37.0 32.0 37.0 5 34.79389464897084 37.0 35.0 37.0 32.0 37.0 6 34.78927450815911 37.0 35.0 37.0 32.0 37.0 7 34.6942169109244 37.0 35.0 37.0 30.0 37.0 8 34.647439513479206 37.0 35.0 37.0 30.0 37.0 9 36.110422859712294 39.0 35.0 39.0 30.0 39.0 10-11 35.953827629080536 38.5 35.0 39.0 30.0 39.0 12-13 35.760891241600014 38.0 35.0 39.0 30.0 39.0 14-15 36.714403197284156 39.0 36.0 41.0 30.0 41.0 16-17 36.5149137425416 39.0 36.0 40.5 30.0 41.0 18-19 36.34512136020423 39.0 35.5 40.0 29.0 41.0 20-21 36.310481681263944 39.0 35.5 40.0 29.0 41.0 22-23 36.40445726081075 39.0 36.0 41.0 29.0 41.0 24-25 36.30623698632603 39.0 35.5 41.0 29.0 41.0 26-27 36.09276722457709 39.0 35.0 40.5 27.5 41.0 28-29 35.8832922001332 39.0 35.0 40.0 27.0 41.0 30-31 35.616012095775886 38.0 34.5 40.0 27.0 41.0 32-33 35.30645417098121 38.0 34.0 40.0 26.0 41.0 34-35 35.03120089359854 38.0 34.0 40.0 25.0 41.0 36-37 34.72242459583107 38.0 33.5 40.0 24.5 41.0 38-39 34.38137048102241 38.0 33.0 40.0 24.0 41.0 40-41 34.263390478312274 38.0 33.0 40.0 24.0 41.0 42-43 34.02684769123712 38.0 33.0 40.0 23.0 41.0 44-45 33.711600173747726 38.0 32.5 40.0 21.5 41.0 46-47 33.301462489883676 37.5 32.0 40.0 18.5 41.0 48-49 32.900547257784766 37.0 31.0 40.0 16.0 41.0 50-51 32.11016203435627 36.0 30.5 39.0 14.5 40.5 52-53 32.11576994931905 36.5 30.5 39.0 13.5 40.0 54-55 32.14501669044547 36.5 31.0 39.0 9.5 41.0 56-57 31.770734512051117 36.0 30.0 39.0 8.0 41.0 58-59 31.279512915439945 36.0 29.5 39.0 4.5 41.0 60-61 30.742747917387646 35.0 28.5 39.0 2.0 40.0 62-63 30.10736970873483 35.0 28.0 38.5 2.0 40.0 64-65 29.559039075917497 34.0 27.5 38.0 2.0 40.0 66-67 28.957369657792377 34.0 26.0 37.5 2.0 40.0 68-69 28.29240797840312 34.0 26.0 37.0 2.0 39.0 70-71 27.618566180528504 33.0 24.5 36.0 2.0 39.0 72-73 26.942826606495707 33.0 22.5 36.0 2.0 39.0 74-75 26.20795133569412 32.0 21.0 35.0 2.0 38.0 76-77 25.42801423943428 31.5 18.5 35.0 2.0 37.0 78-79 24.602708644153047 31.0 11.0 35.0 2.0 36.5 80-81 23.808091631207887 30.5 7.0 34.5 2.0 36.0 82-83 23.048633061176112 30.0 2.0 34.0 2.0 35.5 84-85 22.292003495331464 29.0 2.0 34.0 2.0 35.0 86-87 21.54457396657292 29.0 2.0 34.0 2.0 35.0 88-89 20.76261462476299 28.5 2.0 34.0 2.0 35.0 90-91 19.933488854980084 26.5 2.0 34.0 2.0 35.0 92-93 19.058039585002405 25.5 2.0 33.0 2.0 35.0 94-95 18.15145089198536 24.5 2.0 33.0 2.0 35.0 96-97 17.09456311583057 19.0 2.0 32.5 2.0 34.5 98-99 15.863955803686807 2.0 2.0 32.0 2.0 34.0 100-101 13.225930285593577 2.0 2.0 28.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7309029490993737 1101 2 0.6559203558574573 1101 3 0.6762590462616878 1101 4 0.525865648577053 1101 5 0.6501630412110444 1101 6 0.5359744896771517 1101 7 0.586876459843964 1101 8 0.5635766558672017 1101 9 0.7686118185561526 1101 10-11 0.7647752159808832 1101 12-13 0.8563684543335697 1101 14-15 1.0764806176783637 1101 16-17 1.1197817331647855 1101 18-19 1.114479153418145 1101 20-21 1.1646968541749274 1101 22-23 1.1224998133234436 1101 24-25 1.2213901133056027 1101 26-27 1.2469367472775232 1101 28-29 1.314917148613354 1101 30-31 1.2800795080696474 1101 32-33 1.3795875724569981 1101 34-35 1.4382718605631055 1101 36-37 1.5137760935340268 1101 38-39 1.718459604034031 1101 40-41 1.94240552206076 1101 42-43 2.0290454533524525 1101 44-45 1.9784622888210919 1101 46-47 2.0315807739669935 1101 48-49 2.0146348052658354 1101 50-51 2.0284735068419764 1101 52-53 2.282775968834507 1101 54-55 2.573986049873355 1101 56-57 2.6331143098988363 1101 58-59 2.7961377015462325 1101 60-61 2.948057280517393 1101 62-63 2.7344670363161576 1101 64-65 2.7208477133280837 1101 66-67 3.0014901114419033 1101 68-69 3.2965282122965114 1101 70-71 3.314981321353404 1101 72-73 3.462833432411948 1101 74-75 3.6774179309965263 1101 76-77 4.074085940522977 1101 78-79 4.356539822686411 1101 80-81 4.717841969133758 1101 82-83 4.6676551010469005 1101 84-85 4.822021089440135 1101 86-87 5.082686239776681 1101 88-89 5.523916669925175 1101 90-91 5.7087295181907045 1101 92-93 5.78812023509218 1101 94-95 5.963572142557229 1101 96-97 6.0431703355029995 1101 98-99 6.479741449581637 1101 100-101 6.214824998714478 1102 1 0.6508754693106127 1102 2 0.6746354582535581 1102 3 0.6553953008369291 1102 4 0.6130790210780219 1102 5 0.6024675422472754 1102 6 0.6052821033371529 1102 7 0.6333875633677621 1102 8 0.7568447213666545 1102 9 0.8141365262831926 1102 10-11 0.8439990226894665 1102 12-13 0.800204134376294 1102 14-15 1.1157870158553749 1102 16-17 1.1668610182793344 1102 18-19 1.1431752924117617 1102 20-21 1.1487817656014343 1102 22-23 0.9731491761021474 1102 24-25 1.030924529953154 1102 26-27 1.009546249152315 1102 28-29 1.0335741319767138 1102 30-31 1.1072739852138298 1102 32-33 1.1467216463676948 1102 34-35 1.1763028555126525 1102 36-37 1.2565625489174579 1102 38-39 1.4009760487449654 1102 40-41 1.7516027789384694 1102 42-43 1.6700463495543474 1102 44-45 1.8896430378121067 1102 46-47 1.827989842297285 1102 48-49 1.7994384794133893 1102 50-51 2.011959882958841 1102 52-53 2.1956735375082914 1102 54-55 2.3027338102025254 1102 56-57 2.1969709293377804 1102 58-59 2.407672941947016 1102 60-61 2.5226000767927097 1102 62-63 2.9245581014755153 1102 64-65 2.9041673641388996 1102 66-67 3.0634594633920074 1102 68-69 3.2348289083773345 1102 70-71 3.372328035406561 1102 72-73 3.57180888699391 1102 74-75 3.6882562766961655 1102 76-77 3.6968406492520245 1102 78-79 4.150181565814428 1102 80-81 4.5982804049205654 1102 82-83 4.65177376847728 1102 84-85 4.921586824454437 1102 86-87 5.2303048471000295 1102 88-89 5.586046329537904 1102 90-91 5.662755775558491 1102 92-93 5.552626277350164 1102 94-95 5.902821142185378 1102 96-97 5.955848223305782 1102 98-99 6.3377959856747434 1102 100-101 6.028917543267399 1103 1 0.5689186353925919 1103 2 0.5836820681669259 1103 3 0.5216842837916467 1103 4 0.5359423679244983 1103 5 0.412488373967669 1103 6 0.5603699275965468 1103 7 0.554622105801684 1103 8 0.4216690535568475 1103 9 0.7269698331664074 1103 10-11 0.6293865186672818 1103 12-13 0.7503883763977157 1103 14-15 0.8758951844156115 1103 16-17 0.9559742516303515 1103 18-19 1.0844703648958074 1103 20-21 1.001086822855875 1103 22-23 0.8742515545295575 1103 24-25 0.9633783389119586 1103 26-27 1.1370998968125363 1103 28-29 1.301037127315233 1103 30-31 1.354553952578577 1103 32-33 1.4147125080869003 1103 34-35 1.4860621011157704 1103 36-37 1.4894318734053869 1103 38-39 1.6488303095249819 1103 40-41 1.626161239834694 1103 42-43 1.7732551263987517 1103 44-45 1.709617388483089 1103 46-47 2.0056467483122447 1103 48-49 2.036259181806848 1103 50-51 2.1298393455164017 1103 52-53 2.13542192098582 1103 54-55 2.317725884168894 1103 56-57 2.434122922703736 1103 58-59 2.834728418523742 1103 60-61 3.0614008600592193 1103 62-63 3.1060742386639255 1103 64-65 3.1577755549480955 1103 66-67 3.147402242650614 1103 68-69 3.368501664916277 1103 70-71 3.587342757020547 1103 72-73 3.633491575060056 1103 74-75 3.8947425706896404 1103 76-77 3.994210371263403 1103 78-79 4.089576795172981 1103 80-81 4.547507234036413 1103 82-83 4.804444634754301 1103 84-85 5.17540290806528 1103 86-87 5.2497151414525725 1103 88-89 5.707885143619105 1103 90-91 5.743317097697545 1103 92-93 5.762650349960502 1103 94-95 5.921181105259809 1103 96-97 6.001714403873251 1103 98-99 6.0037999632241235 1103 100-101 5.624700438107466 1104 1 0.6871850732141915 1104 2 0.6235974454317343 1104 3 0.6392826217869256 1104 4 0.550498893863768 1104 5 0.5175142990135271 1104 6 0.5720935351604979 1104 7 0.5827666926243822 1104 8 0.6058187195920581 1104 9 0.6808691210697617 1104 10-11 0.8319518566170885 1104 12-13 0.9148149305324083 1104 14-15 0.9885100133132241 1104 16-17 1.1657724329492822 1104 18-19 1.3132470685199635 1104 20-21 1.2651824036929114 1104 22-23 1.164039610986542 1104 24-25 1.3053720003543745 1104 26-27 1.3232801898863045 1104 28-29 1.1983425593535273 1104 30-31 1.3786121738181265 1104 32-33 1.5619478954400847 1104 34-35 1.6745482141489205 1104 36-37 1.6662514725892734 1104 38-39 1.8093300443036995 1104 40-41 1.9414334011339847 1104 42-43 1.9771057721596677 1104 44-45 2.045095434946546 1104 46-47 2.060703147660348 1104 48-49 2.1480782252542454 1104 50-51 2.1142015655535147 1104 52-53 2.2361824165616966 1104 54-55 2.455599118367914 1104 56-57 2.8058282991926973 1104 58-59 3.026585492830211 1104 60-61 3.1043381304227005 1104 62-63 3.3356117053957455 1104 64-65 3.3782422349589254 1104 66-67 3.4258276770431877 1104 68-69 3.6535108740898927 1104 70-71 3.8867939398880047 1104 72-73 3.9231565668966866 1104 74-75 3.9617521685139714 1104 76-77 4.249194245177133 1104 78-79 4.555277396462827 1104 80-81 4.7903143380267466 1104 82-83 4.981767740915046 1104 84-85 5.223620891016694 1104 86-87 5.281228014230752 1104 88-89 5.807322816131503 1104 90-91 5.973498647197857 1104 92-93 5.896980368899317 1104 94-95 6.027646776919024 1104 96-97 6.289650836375834 1104 98-99 6.458077869403644 1104 100-101 6.1648407244783545 1105 1 0.5460375398325219 1105 2 0.5468959247237244 1105 3 0.44447794552838715 1105 4 0.5148918953781632 1105 5 0.45710355515776513 1105 6 0.48258948004269797 1105 7 0.4697675440017761 1105 8 0.4713272107554616 1105 9 0.6451735213411851 1105 10-11 0.5957483071132117 1105 12-13 0.5639949720553261 1105 14-15 0.8276805192711976 1105 16-17 0.840839111137953 1105 18-19 0.9086802152575402 1105 20-21 0.9476785837711432 1105 22-23 0.8677103950028382 1105 24-25 0.6820827954262612 1105 26-27 0.9441408577578727 1105 28-29 1.1098577061962729 1105 30-31 1.1203349478931273 1105 32-33 1.1479629601828591 1105 34-35 1.2450466697832354 1105 36-37 1.3012499071088754 1105 38-39 1.3821861560001736 1105 40-41 1.4651476964152295 1105 42-43 1.548621933889386 1105 44-45 1.7713742644389825 1105 46-47 1.9171619423865707 1105 48-49 1.9903437337223977 1105 50-51 1.7444440694568542 1105 52-53 1.9155984952478846 1105 54-55 2.1790257933025003 1105 56-57 2.3611509728376205 1105 58-59 2.255525811608269 1105 60-61 2.51890674075133 1105 62-63 2.85135131347678 1105 64-65 2.924085093405324 1105 66-67 3.048725425570538 1105 68-69 2.9922184097867017 1105 70-71 3.2673815571393767 1105 72-73 3.3757565703716956 1105 74-75 3.49296161115117 1105 76-77 3.7085382415741677 1105 78-79 4.000637355121718 1105 80-81 4.243791091074254 1105 82-83 4.598942999811907 1105 84-85 4.609153838424493 1105 86-87 4.919288714827243 1105 88-89 5.17571919001632 1105 90-91 5.184187224990268 1105 92-93 5.417491614180584 1105 94-95 5.682284137209535 1105 96-97 5.822442674199198 1105 98-99 5.807453800220111 1105 100-101 5.242070918447185 1106 1 0.23786112274860827 1106 2 0.15369641232445375 1106 3 0.08007330081653308 1106 4 0.158550187853713 1106 5 0.23316011848539375 1106 6 0.06154871444931587 1106 7 0.16537911461557542 1106 8 0.20517235599284334 1106 9 0.09004221280137159 1106 10-11 0.0893490039203968 1106 12-13 0.09069652015569574 1106 14-15 0.49604824342536347 1106 16-17 0.23685325813104185 1106 18-19 0.37630741063601647 1106 20-21 0.3636703725091337 1106 22-23 0.4103539517498547 1106 24-25 0.4190480673375987 1106 26-27 0.6035531856953824 1106 28-29 0.41359704335319947 1106 30-31 0.690856439703083 1106 32-33 0.7449993960606704 1106 34-35 0.7510982653703664 1106 36-37 0.756281073576325 1106 38-39 0.5898797529774527 1106 40-41 0.7220054373962839 1106 42-43 0.47500336280049993 1106 44-45 0.9065001146254374 1106 46-47 1.349281202843585 1106 48-49 1.2059481778990957 1106 50-51 0.8645141517931023 1106 52-53 1.427924687083454 1106 54-55 1.3301833316033935 1106 56-57 1.363468662238148 1106 58-59 0.6349303171229899 1106 60-61 1.9978282436057846 1106 62-63 2.2896443663529773 1106 64-65 2.3785033931282484 1106 66-67 2.525678030542295 1106 68-69 1.9219254522947686 1106 70-71 2.3945432202394024 1106 72-73 2.7503693284968165 1106 74-75 2.9602837708335628 1106 76-77 3.253627161162491 1106 78-79 3.6281656657659163 1106 80-81 4.024603005162692 1106 82-83 4.294486683296785 1106 84-85 4.305080314039664 1106 86-87 4.709171433251516 1106 88-89 4.611748178619042 1106 90-91 4.935546051480806 1106 92-93 5.182007993119917 1106 94-95 5.048031520873817 1106 96-97 5.483020093593787 1106 98-99 5.347999815827533 1106 100-101 4.738460121860756 1107 1 0.47371482761759154 1107 2 0.34973278493128745 1107 3 0.4042283949206933 1107 4 0.4066647407220856 1107 5 0.4195364190382662 1107 6 0.30128049747481356 1107 7 0.389507890951478 1107 8 0.30836604025613923 1107 9 0.4628512091247998 1107 10-11 0.3681560768700862 1107 12-13 0.47676597795690157 1107 14-15 0.6323957197558627 1107 16-17 0.7639655063808135 1107 18-19 0.8085668602978444 1107 20-21 0.836402790063282 1107 22-23 0.8039528307052706 1107 24-25 0.7486650877440937 1107 26-27 0.7518861298687298 1107 28-29 0.9694322664210162 1107 30-31 1.1572887529095937 1107 32-33 1.1508485695244417 1107 34-35 1.2696445417689333 1107 36-37 1.137319649224409 1107 38-39 1.4752657073362414 1107 40-41 1.4406190252798226 1107 42-43 1.62689266855228 1107 44-45 1.732178539200298 1107 46-47 1.6826633460729283 1107 48-49 1.8715184357868466 1107 50-51 1.919974725929393 1107 52-53 2.0004982416179544 1107 54-55 2.18032755165423 1107 56-57 2.3083370602050124 1107 58-59 2.5000286690634503 1107 60-61 2.646431456088454 1107 62-63 2.8908135942199955 1107 64-65 2.939182338781727 1107 66-67 3.0039226683794666 1107 68-69 3.2492815521466234 1107 70-71 3.2641279601552142 1107 72-73 3.603399268750863 1107 74-75 3.790545873379198 1107 76-77 3.980547665672134 1107 78-79 4.237979357073666 1107 80-81 4.532720453645165 1107 82-83 4.785311082440369 1107 84-85 4.897885405571412 1107 86-87 5.1641235269981784 1107 88-89 5.311997702722554 1107 90-91 5.356699765716016 1107 92-93 5.46829798590397 1107 94-95 5.545498188331994 1107 96-97 5.489019258548808 1107 98-99 5.592189624321248 1107 100-101 5.308790235060139 1108 1 0.3356332308617809 1108 2 0.37275455393246304 1108 3 0.3075129592311683 1108 4 0.32352896890618865 1108 5 0.2681277857806208 1108 6 0.1491834232077096 1108 7 0.21114951011697514 1108 8 0.21275255008016813 1108 9 0.10033187323313797 1108 10-11 0.26794831913596084 1108 12-13 0.3523763762754868 1108 14-15 0.36863923243264196 1108 16-17 0.6067634110818432 1108 18-19 0.6949335602396829 1108 20-21 0.5228931643005765 1108 22-23 0.5328344864074666 1108 24-25 0.6108939335794972 1108 26-27 0.5890971676739127 1108 28-29 0.7592420512070035 1108 30-31 0.7902370494501341 1108 32-33 0.8006636707969079 1108 34-35 0.9315180439541351 1108 36-37 1.068904135013021 1108 38-39 1.038818773066808 1108 40-41 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0.20814075829292022 2311 42-43 0.32983657610984096 2311 44-45 0.503121121283165 2311 46-47 0.6454476027431539 2311 48-49 0.7319305085019963 2311 50-51 0.8351209981649461 2311 52-53 0.882144124014026 2311 54-55 0.8846001083273904 2311 56-57 0.9589406406660572 2311 58-59 1.1113381815357464 2311 60-61 1.0085246923377333 2311 62-63 1.4251205943934657 2311 64-65 1.5064710180358993 2311 66-67 1.57621003792865 2311 68-69 1.353317747518151 2311 70-71 1.542236236200477 2311 72-73 1.5669547563123523 2311 74-75 1.4688594423529295 2311 76-77 1.846156113238571 2311 78-79 2.125138134892609 2311 80-81 2.0660262309494435 2311 82-83 2.2849034252435096 2311 84-85 2.517785722583504 2311 86-87 2.77323434278566 2311 88-89 2.7717159799577153 2311 90-91 2.943512060517808 2311 92-93 3.054600939611273 2311 94-95 3.1423689969432367 2311 96-97 3.216672762833973 2311 98-99 3.555237665201245 2311 100-101 3.4083167546916844 2312 1 -0.31174323932715 2312 2 -0.32016717641327475 2312 3 -0.18281049730753907 2312 4 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64-65 1.3467827872657665 2312 66-67 1.3863837245431867 2312 68-69 1.5055010300351093 2312 70-71 1.715144891641991 2312 72-73 1.6170667995993213 2312 74-75 1.5407024177853899 2312 76-77 1.7059098327243376 2312 78-79 1.9006994249129505 2312 80-81 2.1343799559007444 2312 82-83 2.332352878886091 2312 84-85 2.486791699526794 2312 86-87 2.7264916987983234 2312 88-89 2.6349937926541322 2312 90-91 2.8842426256922558 2312 92-93 3.055911397114855 2312 94-95 3.084337796596568 2312 96-97 3.381814319962384 2312 98-99 3.496136880898236 2312 100-101 3.3080322545335736 2313 1 -0.451062743751379 2313 2 -0.3747875536605392 2313 3 -0.3016734421787106 2313 4 -0.36060063355485994 2313 5 -0.16735624543288452 2313 6 -0.2164581979299811 2313 7 -0.259682389380977 2313 8 -0.25144230982947136 2313 9 -0.21779703456414268 2313 10-11 -0.19380227168449693 2313 12-13 -0.2269254334530757 2313 14-15 -0.18593101185276595 2313 16-17 -0.12493675991461828 2313 18-19 -0.15739338774674394 2313 20-21 -0.040238243864770595 2313 22-23 -0.08638203884263618 2313 24-25 -0.028264369172880777 2313 26-27 -0.0076658216202147855 2313 28-29 -0.05798872117339471 2313 30-31 -0.0037307662016417 2313 32-33 0.08804301913164636 2313 34-35 0.15341313928875167 2313 36-37 0.23596489942616472 2313 38-39 0.230566695423029 2313 40-41 0.19842367384212167 2313 42-43 0.36542138984413697 2313 44-45 0.5306491811845788 2313 46-47 0.5337118753364365 2313 48-49 0.49967368532023926 2313 50-51 0.7685455865605206 2313 52-53 0.8096517854557206 2313 54-55 0.8929596993220841 2313 56-57 0.9116585023972732 2313 58-59 1.045678738285165 2313 60-61 1.2646246069173408 2313 62-63 1.4244189619476124 2313 64-65 1.4266887566306679 2313 66-67 1.513451807066751 2313 68-69 1.6764123263159618 2313 70-71 1.919210810045353 2313 72-73 1.9984382041249695 2313 74-75 2.0219143251005427 2313 76-77 2.199587489346726 2313 78-79 2.4243473830577784 2313 80-81 2.5840445269647496 2313 82-83 2.713367135706658 2313 84-85 2.8075443712262675 2313 86-87 2.898493248199795 2313 88-89 2.9308336303063314 2313 90-91 2.958072430048805 2313 92-93 3.2552566828815017 2313 94-95 3.2365194663432924 2313 96-97 3.318944252498458 2313 98-99 3.523183874884852 2313 100-101 3.179125881820104 2314 1 -0.5499176116146209 2314 2 -0.3277101641180593 2314 3 -0.6095631678493163 2314 4 -0.4445247808201742 2314 5 -0.4673780226794051 2314 6 -0.3789131184096419 2314 7 -0.33087443083233836 2314 8 -0.3480619336373181 2314 9 -0.6460536939612354 2314 10-11 -0.44369741562561416 2314 12-13 -0.4564401557016069 2314 14-15 -0.5697158724979374 2314 16-17 -0.5247340016350961 2314 18-19 -0.54156838828807 2314 20-21 -0.461518171720364 2314 22-23 -0.5006070640944174 2314 24-25 -0.4361852488019409 2314 26-27 -0.44316601483008355 2314 28-29 -0.4604991998635981 2314 30-31 -0.3088204034659725 2314 32-33 -0.2578615469264207 2314 34-35 -0.26584025504757847 2314 36-37 -0.1150299906950849 2314 38-39 -0.039475597077156976 2314 40-41 0.0276122238752734 2314 42-43 0.22694586005753337 2314 44-45 0.25749945779757866 2314 46-47 0.45392464180126524 2314 48-49 0.4457056525629852 2314 50-51 0.49973719494705904 2314 52-53 0.6216788541057454 2314 54-55 0.5941005675827142 2314 56-57 0.6501738665165533 2314 58-59 0.9586756336682534 2314 60-61 0.9177052955743505 2314 62-63 1.061263249102403 2314 64-65 1.2779799718344123 2314 66-67 1.2899089915024788 2314 68-69 1.5032143934659992 2314 70-71 1.522157234298735 2314 72-73 1.563190552571598 2314 74-75 1.6897732077799432 2314 76-77 1.9452526678538504 2314 78-79 2.209492334337508 2314 80-81 2.4098762999769683 2314 82-83 2.6387887944231494 2314 84-85 2.895956653516997 2314 86-87 2.975663873546356 2314 88-89 3.1748120022477764 2314 90-91 3.0355559781164736 2314 92-93 3.1446920378930656 2314 94-95 3.1589013724752526 2314 96-97 3.1502181908958455 2314 98-99 3.215876449702284 2314 100-101 3.0296856562601047 2315 1 -0.5707680139611391 2315 2 -0.5358714795355333 2315 3 -0.482751965600972 2315 4 -0.5061675040465303 2315 5 -0.4789574175956446 2315 6 -0.2900186761222656 2315 7 -0.4937994020893086 2315 8 -0.49897395354904717 2315 9 -0.5356203583636798 2315 10-11 -0.48598593946241664 2315 12-13 -0.5196903362103527 2315 14-15 -0.6118043901291017 2315 16-17 -0.48207859010450704 2315 18-19 -0.47217909346998255 2315 20-21 -0.4626646782926187 2315 22-23 -0.5288455363433044 2315 24-25 -0.5139242086969844 2315 26-27 -0.4996180808480659 2315 28-29 -0.652253856681746 2315 30-31 -0.5792129998540076 2315 32-33 -0.5426940043415982 2315 34-35 -0.6283602381972955 2315 36-37 -0.329805487572834 2315 38-39 -0.35728447947714415 2315 40-41 -0.22194841937598397 2315 42-43 0.010820635766854991 2315 44-45 -0.09135860600258638 2315 46-47 0.05939080720850143 2315 48-49 0.03833773702458387 2315 50-51 0.17653778889980742 2315 52-53 0.16174131306673445 2315 54-55 0.3060228662200757 2315 56-57 0.5503864237754925 2315 58-59 0.7954855249045671 2315 60-61 0.9452786179052488 2315 62-63 0.9744461598789513 2315 64-65 1.0013063978954264 2315 66-67 1.1205845204986247 2315 68-69 1.3647335069870614 2315 70-71 1.3499463005702559 2315 72-73 1.637330798432476 2315 74-75 1.7552588175361095 2315 76-77 1.7843551529344488 2315 78-79 2.142363487013384 2315 80-81 2.367005368132542 2315 82-83 2.4568899290775015 2315 84-85 2.639620258453448 2315 86-87 2.813240944421235 2315 88-89 2.9611526825595895 2315 90-91 3.054046279283053 2315 92-93 3.0859030719740694 2315 94-95 3.30487068137624 2315 96-97 3.4419204631396525 2315 98-99 3.28418632598356 2315 100-101 3.0159927086941547 2316 1 -0.9032810378561038 2316 2 -0.8794060652806905 2316 3 -0.8932191519898893 2316 4 -0.5882987448269432 2316 5 -0.743557170164884 2316 6 -0.5992827356756862 2316 7 -0.6121631746435483 2316 8 -0.7571788033664717 2316 9 -0.6807738525402911 2316 10-11 -0.7124860429855602 2316 12-13 -0.6858560874894977 2316 14-15 -1.1976205235329473 2316 16-17 -1.053418847627583 2316 18-19 -0.9753281043637259 2316 20-21 -0.7150286307550928 2316 22-23 -0.9897149732552037 2316 24-25 -1.2398070637647791 2316 26-27 -0.9950083118688369 2316 28-29 -0.6916209763262273 2316 30-31 -0.7396479242175857 2316 32-33 -0.8320436645370677 2316 34-35 -0.7752533197577876 2316 36-37 -0.6864787356926669 2316 38-39 -0.6925235021806913 2316 40-41 -0.6465346541566177 2316 42-43 -0.45912538986475937 2316 44-45 -0.5321484430091346 2316 46-47 -0.3139704954030407 2316 48-49 -0.12127711512140138 2316 50-51 -0.07895600701783678 2316 52-53 0.03396243774751184 2316 54-55 0.09512733502297976 2316 56-57 0.33750421603575376 2316 58-59 0.47928451312949605 2316 60-61 0.47796963329176734 2316 62-63 0.8772361228971768 2316 64-65 0.9404745936872061 2316 66-67 1.0471783072500536 2316 68-69 1.1330967197002728 2316 70-71 0.9327359238883339 2316 72-73 1.1935190059790202 2316 74-75 1.4625687092818325 2316 76-77 1.4982704097031636 2316 78-79 1.6327351227720825 2316 80-81 1.9318039781326704 2316 82-83 2.2102644420395308 2316 84-85 2.283951640313802 2316 86-87 2.5687722686654055 2316 88-89 2.2973615586777747 2316 90-91 2.5849154391210796 2316 92-93 2.7338007360705916 2316 94-95 2.80895486400739 2316 96-97 2.842112336790933 2316 98-99 2.7731105829084637 2316 100-101 2.4309536156593943 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11071.0 3 4336.0 4 4912.0 5 5734.0 6 6860.0 7 8355.0 8 10548.0 9 13147.0 10 16312.0 11 19430.0 12 23152.0 13 26520.0 14 29862.0 15 34056.0 16 38056.0 17 42909.0 18 43704.0 19 43983.0 20 47973.0 21 51895.0 22 56864.0 23 62753.0 24 69585.0 25 77205.0 26 84709.0 27 94023.0 28 103678.0 29 114023.0 30 127030.0 31 142240.0 32 161651.0 33 185445.0 34 214347.0 35 248644.0 36 284730.0 37 280173.0 38 144685.0 39 9899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88671731958517 17.973236047028525 14.037398681389055 24.10264795199725 2 15.899808458145829 19.219448258898815 39.83795382475585 25.042789458199504 3 19.77508162634331 22.828977097058054 28.67902924958125 28.716912027017383 4 13.309441003749656 15.666973814930941 35.6130597811093 35.41052540021011 5 14.649068347253838 36.39693201770706 33.56807727112551 15.38592236391359 6 34.0475982546106 34.56655291030192 16.83156978686228 14.5542790482252 7 30.28081933041453 31.232453887525512 19.72947393595482 18.75725284610514 8 26.958138814357724 34.95138242945849 18.979639120446965 19.110839635736816 9 26.634114554578865 16.57171356904623 19.424111642963215 37.37006023341169 10-11 25.803433584142958 25.777275143145275 26.76327850875144 21.656012763960327 12-13 26.45263362089027 22.908338381122277 27.146952318863082 23.492075679124373 14-15 23.184709394520393 24.613091072644234 25.103440685174792 27.098758847660577 16-17 23.287416235477348 27.728865778896477 25.808228391491877 23.175489594134298 18-19 23.2713336269497 26.462653679379418 27.650169401245112 22.61584329242577 20-21 23.69052177319376 25.766324756647744 27.831187887884862 22.711965582273635 22-23 23.472935782465733 25.536431404962606 27.723059696498098 23.26757311607356 24-25 23.29592677892937 25.786482263883727 27.747692983093998 23.16989797409291 26-27 23.010327394598686 26.125330274975312 27.654299762689583 23.21004256773642 28-29 22.982745159859018 26.35005160687992 27.676643996784676 22.990559236476383 30-31 23.175790763454103 25.969102796063947 27.637255553133944 23.217850887348003 32-33 22.993112185142234 25.982310703996202 27.596205442340267 23.428371668521297 34-35 23.489053594463048 26.094767950063343 27.396385136546826 23.019793318926784 36-37 23.525084318750697 25.863027213010497 27.36928215595096 23.242606312287844 38-39 23.18762625499203 26.080294353525186 27.38572044363631 23.346358947846475 40-41 23.475242012541614 25.877211605188794 27.42407418038091 23.223472201888683 42-43 23.216076322415056 25.901753165494313 27.387809908652784 23.49436060343785 44-45 23.223795432642298 26.101273239053924 27.415184617540046 23.259746710763732 46-47 23.44384013543285 26.328004152114676 26.88737117201453 23.34078454043794 48-49 23.39322682707707 26.192030542285206 27.011920390338013 23.402822240299717 50-51 23.43201222960326 26.43670158008729 26.88619248311416 23.245093707195284 52-53 23.548274306204746 26.324321377790433 26.76840671838917 23.358997597615648 54-55 23.212287922312843 26.319760542495015 26.96979134754962 23.49816018764252 56-57 23.241182861690064 26.495295567681975 26.97005214391898 23.293469426708977 58-59 23.36735387018986 26.152532011004315 26.947610637502972 23.532503481302857 60-61 23.238072207397963 26.33001852216334 27.09829686780657 23.333612402632127 62-63 23.156254144548964 26.39933133619494 27.159508480479506 23.28490603877659 64-65 23.000012566735307 26.268671366625675 27.1125446246469 23.61877144199212 66-67 23.00031534218703 26.33789908744352 26.972438209827704 23.689347360541742 68-69 23.094161151042485 26.093002127000165 27.202965063703864 23.609871658253482 70-71 23.094598560376944 26.135676592946446 27.001406879648272 23.768317967028338 72-73 23.168274121589146 26.24957754420289 26.841881800689176 23.74026653351879 74-75 23.066704730507833 26.531153957651654 26.76009743301735 23.642043878823163 76-77 23.255341999364177 26.226931040385626 26.78341195075361 23.734315009496587 78-79 23.303405356672524 26.260632028193694 26.84047207079737 23.59549054433641 80-81 23.339378323251463 26.417856942106837 26.660528276305 23.582236458336695 82-83 23.37327537045082 26.382836936426845 26.627590744189472 23.61629694893286 84-85 23.401509380390554 26.22746262634633 26.65464831898796 23.716379674275153 86-87 23.429910796840915 26.23181817641512 26.62389366330006 23.714377363443905 88-89 23.43166437551398 26.257408032044204 26.54428616412126 23.76664142832055 90-91 23.409160589303898 26.25295021389316 26.54909690969606 23.788792287106876 92-93 23.483360531802678 25.908906951004884 26.68539225661361 23.922340260578828 94-95 23.392151464316182 25.91164253187622 26.713297236593526 23.982908767214074 96-97 23.51589956872767 25.805720757067814 26.62130491767306 24.057074756531456 98-99 23.496256850326493 25.815258695195475 26.340969083541843 24.34751537093619 100-101 24.012597735630454 25.260557521874183 26.592352627808918 24.134492114686445 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 453.0 1 318.5 2 267.5 3 353.5 4 303.0 5 294.0 6 539.0 7 1178.0 8 1919.5 9 2207.5 10 2001.5 11 1761.5 12 1787.5 13 1917.5 14 2096.0 15 2288.0 16 2444.0 17 2670.0 18 2857.5 19 3088.0 20 3497.5 21 4121.5 22 4905.5 23 5925.5 24 7331.5 25 9289.5 26 11706.5 27 14708.0 28 17838.0 29 21163.0 30 25008.5 31 29973.5 32 36291.5 33 40600.0 34 43529.5 35 47666.5 36 53612.5 37 59855.0 38 64959.5 39 69572.5 40 74822.5 41 81901.0 42 88255.0 43 93487.5 44 99424.5 45 105717.5 46 109536.0 47 112314.0 48 114679.5 49 114942.0 50 114643.5 51 113056.0 52 112008.0 53 112528.5 54 112955.0 55 108386.0 56 100000.0 57 92602.0 58 85435.5 59 76011.0 60 64246.5 61 54146.5 62 45121.0 63 36013.5 64 28538.5 65 22439.5 66 17136.5 67 13264.5 68 10051.5 69 7389.0 70 5575.5 71 4213.5 72 3176.5 73 2432.5 74 1808.0 75 1361.5 76 1010.5 77 628.5 78 423.0 79 290.0 80 164.5 81 96.0 82 57.0 83 37.0 84 29.0 85 19.0 86 12.0 87 9.0 88 9.0 89 5.5 90 3.5 91 3.5 92 2.5 93 2.5 94 2.0 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.30001708270235444 2 0.03453898269281124 3 0.04105961659351896 4 0.04398031719487763 5 0.05834608875737434 6 0.056376313933202214 7 0.028086272061902548 8 0.03100697266326122 9 0.03790118454786366 10-11 0.030514528957218186 12-13 0.03280693931293575 14-15 0.0265919601263237 16-17 0.02059773156655852 18-19 0.025420283722290278 20-21 0.038444570706255975 22-23 0.031448473916954975 24-25 0.051655646682169015 26-27 0.04440483763112163 28-29 0.037120066945174716 30-31 0.03713704776262448 32-33 0.03285788176528503 34-35 0.03158432045655305 36-37 0.030803202853864103 38-39 0.01888266900413279 40-41 0.01833928284574048 42-43 0.019256246988027505 44-45 0.008031926653736341 46-47 0.015520467149080371 48-49 0.013041267801415454 50-51 0.005705554663119261 52-53 0.004499916624186322 54-55 0.006011209377214935 56-57 0.005026321965128872 58-59 0.0067583653450043624 60-61 0.006401768178559408 62-63 0.00405841537049257 64-65 0.007403636408095231 66-67 0.0026659883396122734 68-69 0.007182885781248355 70-71 0.013414845785310166 72-73 0.013720500499405841 74-75 0.010952627255095009 76-77 0.009611142676563994 78-79 0.00517914932217671 80-81 0.00636780654365989 82-83 0.010731876628248134 84-85 0.0038037031087461734 86-87 0.001375446213430536 88-89 0.019222285353127987 90-91 0.029088140291438375 92-93 0.013567673142358003 94-95 0.022092043502137376 96-97 0.018186455488692643 98-99 0.013160133523563771 100-101 0.10249621412674957 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2944499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.74168930778261 #Duplication Level Percentage of deduplicated Percentage of total 1 78.09583002192284 44.312893233373465 2 12.34263018119521 14.006833739644783 3 3.9029454346400727 6.643791517127267 4 1.7963097270576898 4.077025937330209 5 0.9888112861956465 2.8053411392671146 6 0.6208586477212107 2.1137141095828196 7 0.39720759588401505 1.5776760997439436 8 0.30116153864555684 1.3670731565823964 9 0.22927442290145433 1.170847626344593 >10 1.198419053871097 12.237673587348413 >50 0.08004909001664097 3.1376820348899463 >100 0.04301079861584057 4.3342635950065915 >500 0.002227783608904749 0.8826709173288141 >1k 0.0011439969881934008 0.9204908857045135 >5k 1.2042073559930533E-4 0.4120224207251024 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6627 0.2250637544791151 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5433 0.18451356240908895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3622 0.1230090416060593 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.396163489951941E-5 2 3.396163489951941E-5 0.0 0.0 0.0 3.396163489951941E-5 3 3.396163489951941E-5 0.0 0.0 0.0 3.396163489951941E-5 4 3.396163489951941E-5 0.0 0.0 0.0 3.396163489951941E-5 5 3.396163489951941E-5 0.0 0.0 0.0 3.396163489951941E-5 6 6.792326979903882E-5 0.0 0.0 0.0 3.396163489951941E-5 7 1.0188490469855823E-4 0.0 0.0 0.0 3.396163489951941E-5 8 1.0188490469855823E-4 0.0 0.0 0.0 3.396163489951941E-5 9 1.0188490469855823E-4 0.0 0.0 0.0 3.396163489951941E-5 10-11 1.3584653959807763E-4 6.792326979903882E-5 0.0 0.0 3.396163489951941E-5 12-13 1.3584653959807763E-4 6.792326979903882E-5 0.0 0.0 3.396163489951941E-5 14-15 2.0376980939711646E-4 6.792326979903882E-5 0.0 0.0 1.1886572214831793E-4 16-17 2.0376980939711646E-4 6.792326979903882E-5 0.0 0.0 2.0376980939711646E-4 18-19 2.0376980939711646E-4 6.792326979903882E-5 0.0 5.0942452349279116E-5 2.3773144429663586E-4 20-21 2.0376980939711646E-4 6.792326979903882E-5 0.0 1.1886572214831793E-4 3.0565471409567467E-4 22-23 2.0376980939711646E-4 6.792326979903882E-5 0.0 2.7169307919615526E-4 3.735779838947135E-4 24-25 3.3961634899519407E-4 6.792326979903882E-5 0.0 4.245204362439926E-4 3.735779838947135E-4 26-27 3.3961634899519407E-4 6.792326979903882E-5 0.0 6.962135154401478E-4 3.735779838947135E-4 28-29 3.9055880134447325E-4 6.792326979903882E-5 0.0 0.0019867556416218856 3.735779838947135E-4 30-31 4.075396187942329E-4 6.792326979903882E-5 0.0 0.00747155967789427 3.735779838947135E-4 32-33 4.075396187942329E-4 6.792326979903882E-5 0.0 0.020801501375955635 3.735779838947135E-4 34-35 4.245204362439926E-4 6.792326979903882E-5 0.0 0.04094075087137065 3.735779838947135E-4 36-37 4.415012536937523E-4 6.792326979903882E-5 0.0 0.07916457095077975 3.735779838947135E-4 38-39 4.415012536937523E-4 6.792326979903882E-5 0.0 0.13309564717121658 4.415012536937523E-4 40-41 4.415012536937523E-4 6.792326979903882E-5 0.0 0.1942775324427008 4.415012536937523E-4 42-43 4.754628885932717E-4 6.792326979903882E-5 0.0 0.2564782667611706 4.415012536937523E-4 44-45 5.094245234927912E-4 6.792326979903882E-5 0.0 0.3347937968394623 4.415012536937523E-4 46-47 5.7734779329183E-4 6.792326979903882E-5 0.0 0.43065051134335586 4.415012536937523E-4 48-49 6.792326979903881E-4 6.792326979903882E-5 0.0 0.5364240232379092 4.754628885932717E-4 50-51 6.792326979903881E-4 6.792326979903882E-5 0.0 0.6463748162251031 4.754628885932717E-4 52-53 6.792326979903881E-4 6.792326979903882E-5 0.0 0.7565463598391442 4.924437060430315E-4 54-55 6.792326979903881E-4 6.792326979903882E-5 0.0 0.8788761687472131 5.094245234927912E-4 56-57 7.131943328899076E-4 6.792326979903882E-5 0.0 1.0229584048084241 5.094245234927912E-4 58-59 7.131943328899076E-4 6.792326979903882E-5 0.0 1.183308263986505 5.094245234927912E-4 60-61 7.131943328899076E-4 6.792326979903882E-5 0.0 1.3532862466585995 5.264053409425509E-4 62-63 7.131943328899076E-4 6.792326979903882E-5 0.0 1.5237227114018377 5.7734779329183E-4 64-65 7.131943328899076E-4 6.792326979903882E-5 0.0 1.7063004606216543 5.7734779329183E-4 66-67 7.131943328899076E-4 6.792326979903882E-5 0.0 1.9167267504590764 5.7734779329183E-4 68-69 7.131943328899076E-4 6.792326979903882E-5 0.0 2.149771489139579 5.7734779329183E-4 70-71 7.980984201387061E-4 6.792326979903882E-5 0.0 2.396010322978544 6.113094281913493E-4 72-73 8.999833248372643E-4 6.792326979903882E-5 0.0 2.6497546781303036 7.131943328899076E-4 74-75 9.16964142287024E-4 6.792326979903882E-5 0.0 2.9079649882713494 7.47155967789427E-4 76-77 9.16964142287024E-4 6.792326979903882E-5 0.0 3.191425773960188 7.47155967789427E-4 78-79 9.16964142287024E-4 6.792326979903882E-5 0.0 3.511972664959302 7.47155967789427E-4 80-81 9.848874120860628E-4 6.792326979903882E-5 0.0 3.847836253298099 7.641367852391867E-4 82-83 0.0010188490469855824 6.792326979903882E-5 0.0 4.194839257883939 8.150792375884658E-4 84-85 0.0010358298644353421 6.792326979903882E-5 0.0 4.545102579420132 8.150792375884658E-4 86-87 0.0010528106818851017 6.792326979903882E-5 0.0 4.908746785106736 8.150792375884658E-4 88-89 0.0010528106818851017 6.792326979903882E-5 0.0 5.297760332063282 8.150792375884658E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4450 0.0 44.56792 1 GTATCAA 7415 0.0 41.9204 1 ATCAACG 9545 0.0 32.36374 3 TATCAAC 9645 0.0 32.027103 2 TCAACGC 9795 0.0 31.489195 4 CAACGCA 10055 0.0 30.62769 5 AACGCAG 10460 0.0 29.528671 6 ACGCAGA 11860 0.0 25.675417 7 CGCAGAG 11935 0.0 25.354858 8 GTACATG 9165 0.0 24.60468 1 TACATGG 9250 0.0 23.4277 2 GCAGAGT 12985 0.0 22.644917 9 ACATGGG 9580 0.0 21.92694 3 GAGTACT 7265 0.0 21.346252 12-13 TGGTATC 2495 0.0 20.761679 2 AGTACTT 7620 0.0 20.195942 12-13 GTGGTAT 2755 0.0 19.900557 1 GTACTTT 7760 0.0 19.803333 14-15 AGAGTAC 11945 0.0 19.782995 10-11 CAGAGTA 12390 0.0 19.321657 10-11 >>END_MODULE