FastQCFastQC Report
Thu 26 May 2016
SRR938533_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938533_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2944499
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67200.22822218652477044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58090.19728313713130824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39850.13533711507458485No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49250.045.0996671
GTATCAA76150.038.6619221
ATCAACG95050.030.5119923
TCAACGC97750.029.6692074
TATCAAC98300.029.6598952
CAACGCA98450.029.4099585
AACGCAG103650.027.9065076
ACGCAGA120300.024.0836187
GTACATG93600.023.9335821
TACATGG91550.023.846082
CGCAGAG121000.023.787288
ACATGGG94400.022.2609213
GTAAGGT11300.020.616294
GCAGAGT136850.020.5810439
GCACCGT6750.020.405716
GAGTACT75350.019.95023312-13
GTCCTAC7200.019.8176161
TAAGGTG13450.019.795145
GTACTTT77400.019.2070614-15
AGAGTAC122200.018.88959110-11
Produced by FastQC (version 0.11.5)