##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938533_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2944499 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.780361956312433 31.0 31.0 34.0 30.0 34.0 2 32.014273735531916 31.0 31.0 34.0 30.0 34.0 3 32.087540868582394 33.0 31.0 34.0 30.0 34.0 4 35.7560539161331 37.0 35.0 37.0 35.0 37.0 5 35.62369116104301 37.0 35.0 37.0 33.0 37.0 6 35.66358487471044 37.0 35.0 37.0 33.0 37.0 7 35.59488660040299 37.0 35.0 37.0 33.0 37.0 8 35.57780695459567 37.0 35.0 37.0 33.0 37.0 9 37.14753953049398 39.0 37.0 39.0 33.0 39.0 10-11 37.07647090387873 39.0 37.0 39.0 33.0 39.0 12-13 36.960957364903166 39.0 37.0 39.0 33.0 39.0 14-15 38.01741382829473 40.0 37.0 41.0 33.0 41.0 16-17 37.926165028414005 40.0 37.0 41.0 33.0 41.0 18-19 37.90462520109533 40.0 37.0 41.0 33.0 41.0 20-21 37.88991403970591 40.0 37.0 41.0 32.5 41.0 22-23 37.92113208393007 40.0 37.0 41.0 32.5 41.0 24-25 37.84450835269429 40.0 37.0 41.0 32.0 41.0 26-27 37.66597916997085 40.0 37.0 41.0 32.0 41.0 28-29 37.53452998285957 39.5 36.5 41.0 32.0 41.0 30-31 37.308499850059384 39.0 36.0 41.0 31.0 41.0 32-33 37.10018240794105 39.0 36.0 41.0 31.0 41.0 34-35 36.93337270618873 39.0 36.0 40.5 30.5 41.0 36-37 36.95691287380298 39.0 36.0 41.0 30.5 41.0 38-39 36.81473979104764 39.0 35.0 41.0 30.0 41.0 40-41 36.6471012555956 39.0 35.0 40.5 30.0 41.0 42-43 36.42591710847924 39.0 35.0 40.0 30.0 41.0 44-45 36.152314196744506 38.5 35.0 40.0 30.0 41.0 46-47 35.80702540568022 38.0 35.0 40.0 28.5 41.0 48-49 35.426320063277316 38.0 34.0 40.0 27.5 41.0 50-51 35.053754645527135 38.0 34.0 40.0 26.5 41.0 52-53 34.51893581896275 38.0 33.0 40.0 25.0 41.0 54-55 34.177972721335614 37.0 33.0 40.0 24.0 41.0 56-57 33.78755214384518 37.0 32.5 40.0 23.0 41.0 58-59 33.30585084253722 36.5 32.0 39.0 22.5 41.0 60-61 32.86970992348783 36.0 31.0 39.0 21.5 40.0 62-63 32.364265533797095 35.5 31.0 39.0 19.5 40.0 64-65 31.841038322648437 35.0 30.0 38.0 18.0 40.0 66-67 31.31669530198516 35.0 29.5 38.0 15.5 40.0 68-69 30.727795288774082 34.0 29.0 37.5 12.0 39.5 70-71 30.14010125321829 34.0 28.5 36.5 8.0 39.0 72-73 29.534053161505575 34.0 28.0 36.0 7.0 39.0 74-75 28.8890877531288 33.0 26.5 35.5 4.0 38.0 76-77 27.6316894656782 31.5 26.0 34.5 2.0 36.0 78-79 28.239951855986366 33.0 26.0 35.0 2.0 37.0 80-81 27.916451491408218 33.0 26.0 35.0 2.0 36.5 82-83 27.403990458139056 33.0 26.0 35.0 2.0 36.0 84-85 26.794271453310053 32.0 25.0 35.0 2.0 36.0 86-87 26.122034342684444 32.0 23.5 34.5 2.0 35.0 88-89 25.353507336901796 31.0 20.5 34.0 2.0 35.0 90-91 24.780455690424755 31.0 19.0 34.0 2.0 35.0 92-93 23.961237208774733 31.0 11.0 34.0 2.0 35.0 94-95 23.142111442387993 30.0 2.0 34.0 2.0 35.0 96-97 22.12191751466039 30.0 2.0 34.0 2.0 35.0 98-99 20.921132763162767 29.0 2.0 34.0 2.0 35.0 100-101 17.821713473157914 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3435086421740685 1101 2 0.3531351262556939 1101 3 0.32961810883227116 1101 4 0.2982749711909918 1101 5 0.22246235768893285 1101 6 0.29534247129700475 1101 7 0.33413665071037 1101 8 0.4245746262811352 1101 9 0.4284766388377932 1101 10-11 0.44502373425960684 1101 12-13 0.38666858082563493 1101 14-15 0.5940721176222397 1101 16-17 0.6175787804935808 1101 18-19 0.6923819791362931 1101 20-21 0.6661900863034091 1101 22-23 0.6993113636151733 1101 24-25 0.614880954096158 1101 26-27 0.6288041525638519 1101 28-29 0.5700532704902841 1101 30-31 0.6761249750165703 1101 32-33 0.6352438808101084 1101 34-35 0.5211174924155628 1101 36-37 0.7707115781395757 1101 38-39 0.8155391248544888 1101 40-41 0.9981629033777963 1101 42-43 0.8467645524601153 1101 44-45 0.9544656698562619 1101 46-47 1.012343866637309 1101 48-49 1.106184006237413 1101 50-51 1.2464611264291037 1101 52-53 1.2675920209768847 1101 54-55 1.320358404179558 1101 56-57 1.5280086583185977 1101 58-59 1.4724408104676243 1101 60-61 1.4752975879150156 1101 62-63 1.6540546711026067 1101 64-65 1.6722640540079574 1101 66-67 1.7360096035007153 1101 68-69 1.828533305616137 1101 70-71 1.8425420707710067 1101 72-73 1.9112982280000494 1101 74-75 2.09964113625502 1101 76-77 1.8845051918821838 1101 78-79 2.079220623826913 1101 80-81 2.398562509542355 1101 82-83 2.565514803116489 1101 84-85 2.745418165375334 1101 86-87 2.8354439062869083 1101 88-89 3.050092992978282 1101 90-91 3.1104954148557518 1101 92-93 3.35974455636018 1101 94-95 3.614135555822255 1101 96-97 4.069895570236159 1101 98-99 4.266027367475072 1101 100-101 4.776903956154019 1102 1 0.3259944796625227 1102 2 0.304279781672939 1102 3 0.2814229409575262 1102 4 0.18008772997311695 1102 5 0.15883829443472308 1102 6 0.20872149665043338 1102 7 0.24419317800744267 1102 8 0.2549360303334325 1102 9 0.4061088166083806 1102 10-11 0.4118776066536398 1102 12-13 0.4500746977070733 1102 14-15 0.5938358255951783 1102 16-17 0.6093401807833487 1102 18-19 0.618488152042012 1102 20-21 0.5847607983936456 1102 22-23 0.5803261197920904 1102 24-25 0.5875347884112827 1102 26-27 0.47009369595453165 1102 28-29 0.4961479023116766 1102 30-31 0.5177454072988965 1102 32-33 0.4170409357121798 1102 34-35 0.5538770270523585 1102 36-37 0.6571289627217354 1102 38-39 0.6378662664294339 1102 40-41 0.6047801742035546 1102 42-43 0.6414794830074726 1102 44-45 0.5648806912512967 1102 46-47 0.6535857913806353 1102 48-49 0.6964885214164767 1102 50-51 0.7189457804560817 1102 52-53 0.7209789156153263 1102 54-55 0.6972798112632219 1102 56-57 0.8652463471504177 1102 58-59 0.921063438758118 1102 60-61 1.0105047663894098 1102 62-63 1.0421737318638193 1102 64-65 1.0201021287256005 1102 66-67 1.0543425292269681 1102 68-69 1.21736784689406 1102 70-71 1.404483470615137 1102 72-73 1.4310280785676888 1102 74-75 1.7867478637999028 1102 76-77 1.6762298474942234 1102 78-79 1.9932827079225923 1102 80-81 2.056793577527614 1102 82-83 2.0475178496959074 1102 84-85 2.2105987139650978 1102 86-87 2.3664875552015197 1102 88-89 2.581741981772492 1102 90-91 2.740932483057424 1102 92-93 3.0865267299346186 1102 94-95 3.2024673461192847 1102 96-97 3.8311585677986564 1102 98-99 4.128885462972008 1102 100-101 4.382988135622284 1103 1 0.24410027722882077 1103 2 0.24702418785830105 1103 3 0.2688500884522256 1103 4 0.1847283757230116 1103 5 0.11981090608530565 1103 6 0.1780475021429737 1103 7 0.25773083830610233 1103 8 0.23425143605813048 1103 9 0.32042830302506786 1103 10-11 0.3400906725329591 1103 12-13 0.4275613155323228 1103 14-15 0.6034981788773948 1103 16-17 0.55591713224279 1103 18-19 0.4773592909138884 1103 20-21 0.5094230963749595 1103 22-23 0.41522926991139286 1103 24-25 0.46793804000130734 1103 26-27 0.46990715187237697 1103 28-29 0.4486133707578901 1103 30-31 0.5688453036161363 1103 32-33 0.5767701259192322 1103 34-35 0.6199834655946646 1103 36-37 0.582525090784209 1103 38-39 0.74652841934234 1103 40-41 0.7310894230089886 1103 42-43 0.8555460514014399 1103 44-45 0.9310111020906859 1103 46-47 1.1095240920379936 1103 48-49 1.2312802466527941 1103 50-51 1.1099416622109217 1103 52-53 1.2965987165269368 1103 54-55 1.367395153507637 1103 56-57 1.3995132925679101 1103 58-59 1.5314461569954076 1103 60-61 1.5633849658636123 1103 62-63 1.7121400188919509 1103 64-65 1.6190045087477252 1103 66-67 1.7187798194211403 1103 68-69 1.7455088029720294 1103 70-71 1.7732249198742487 1103 72-73 1.8471812399400882 1103 74-75 2.0270759293152665 1103 76-77 1.9447463249760837 1103 78-79 2.213822568168215 1103 80-81 2.4629557953869217 1103 82-83 2.5805609733376578 1103 84-85 2.8019891097677885 1103 86-87 2.7600690755441484 1103 88-89 3.1504748272404264 1103 90-91 3.1943137601275318 1103 92-93 3.319667993382705 1103 94-95 3.644136857138438 1103 96-97 3.890867592468261 1103 98-99 4.390356798715089 1103 100-101 4.589442535143249 1104 1 0.3239458676118794 1104 2 0.32053693611259604 1104 3 0.3156341010447079 1104 4 0.25368104472953235 1104 5 0.22995483575265752 1104 6 0.22005485634848299 1104 7 0.24801724567285532 1104 8 0.33432072679002545 1104 9 0.37126967832327296 1104 10-11 0.3928848474418345 1104 12-13 0.3957008338437831 1104 14-15 0.5932384565899724 1104 16-17 0.5686007532903474 1104 18-19 0.5955557448530655 1104 20-21 0.6569714959147106 1104 22-23 0.5647719972909186 1104 24-25 0.5308188927758906 1104 26-27 0.4842318372002836 1104 28-29 0.5681333773926767 1104 30-31 0.6742863285449872 1104 32-33 0.7260354714815236 1104 34-35 0.711145528809503 1104 36-37 0.8583581421957973 1104 38-39 0.8804724628809524 1104 40-41 0.8591743380834558 1104 42-43 0.9327407666272336 1104 44-45 1.0189603517977943 1104 46-47 1.072372318095546 1104 48-49 1.1715348055767976 1104 50-51 1.2348487253175406 1104 52-53 1.3435048816996087 1104 54-55 1.4241245385420527 1104 56-57 1.5550106086161009 1104 58-59 1.6942343221675813 1104 60-61 1.6462635762354338 1104 62-63 1.8062719281130697 1104 64-65 1.8573832928935978 1104 66-67 1.991956219421045 1104 68-69 2.088316490668067 1104 70-71 2.069293771367601 1104 72-73 2.295723325486538 1104 74-75 2.3496956686371604 1104 76-77 2.2008525555237917 1104 78-79 2.7234591846110625 1104 80-81 2.873911854264346 1104 82-83 3.113128164553082 1104 84-85 3.265838634327327 1104 86-87 3.32178160486016 1104 88-89 3.555422973500537 1104 90-91 3.7243896224128754 1104 92-93 4.027057537413693 1104 94-95 4.163537289226593 1104 96-97 4.506505287307402 1104 98-99 4.849457938132954 1104 100-101 5.144041941563184 1105 1 0.2725456400332611 1105 2 0.19332442211906908 1105 3 0.19712993856981598 1105 4 0.16731243801399387 1105 5 0.03831506493300907 1105 6 0.21121337283229025 1105 7 0.19311634846445003 1105 8 0.23473707208540873 1105 9 0.26455949195343464 1105 10-11 0.29176027904208013 1105 12-13 0.39854389814423286 1105 14-15 0.38278047216238775 1105 16-17 0.3925125142768664 1105 18-19 0.4641997994852787 1105 20-21 0.3219679524403247 1105 22-23 0.28927730605876434 1105 24-25 0.25717786611992466 1105 26-27 0.4838539586523325 1105 28-29 0.46558192624814154 1105 30-31 0.5055352505152584 1105 32-33 0.5323901482406086 1105 34-35 0.5453142069353376 1105 36-37 0.6918990747618494 1105 38-39 0.6407502933655991 1105 40-41 0.5365845191487253 1105 42-43 0.67278023215232 1105 44-45 0.6603197802300258 1105 46-47 0.7728004116856084 1105 48-49 0.9126359326191249 1105 50-51 0.9798637259682366 1105 52-53 1.01179621133155 1105 54-55 1.0823237838665776 1105 56-57 1.3112254967777446 1105 58-59 1.3537468220194313 1105 60-61 1.3219186472240168 1105 62-63 1.4098328005614533 1105 64-65 1.5298690859144166 1105 66-67 1.5614889215069532 1105 68-69 1.6126730874275843 1105 70-71 1.7542238966301937 1105 72-73 1.8900118477248178 1105 74-75 2.052770522994553 1105 76-77 1.8745731276852986 1105 78-79 2.138639028449642 1105 80-81 2.2913067867105 1105 82-83 2.287375626443904 1105 84-85 2.438397839806612 1105 86-87 2.794417973967416 1105 88-89 2.9817914858615033 1105 90-91 3.1355950101048755 1105 92-93 3.364566482399571 1105 94-95 3.4475744060132705 1105 96-97 3.7317107184945577 1105 98-99 4.0796753805418575 1105 100-101 4.256221586221571 1106 1 0.05277024488453108 1106 2 0.07709367068727602 1106 3 0.08071469388178798 1106 4 0.0925661989609381 1106 5 -0.025705121846733903 1106 6 0.02558029740158929 1106 7 0.00972160865585181 1106 8 0.008115117365100843 1106 9 0.0810359377439056 1106 10-11 0.18140010986963517 1106 12-13 0.041059461981639345 1106 14-15 0.026865940118064202 1106 16-17 -0.025450511312122615 1106 18-19 0.15203932070189552 1106 20-21 0.320421095717073 1106 22-23 0.15833090699746322 1106 24-25 0.17743171149790982 1106 26-27 0.17457269280724574 1106 28-29 0.20254040944659124 1106 30-31 0.24766833028160562 1106 32-33 0.203942006933147 1106 34-35 0.22802248806974745 1106 36-37 0.365059173833707 1106 38-39 0.28099238000828564 1106 40-41 0.41145068452313893 1106 42-43 0.5378992890620395 1106 44-45 0.1988316920821802 1106 46-47 -0.011023865390626497 1106 48-49 0.23298331391021065 1106 50-51 0.17340274246971887 1106 52-53 0.4302014730746535 1106 54-55 0.5453923300140886 1106 56-57 0.35651168837598135 1106 58-59 0.9194547741009345 1106 60-61 0.8777694452343638 1106 62-63 0.9940106725977458 1106 64-65 0.7025252228880596 1106 66-67 0.8267460117756151 1106 68-69 0.5809608416848526 1106 70-71 0.8122829659626909 1106 72-73 1.2865817564069317 1106 74-75 1.5903297331215356 1106 76-77 1.6467832291075304 1106 78-79 1.8149772149710692 1106 80-81 1.7409818868982114 1106 82-83 1.956062705344035 1106 84-85 2.033240779796216 1106 86-87 2.2641294753477474 1106 88-89 2.4299411223287812 1106 90-91 2.3623855416873347 1106 92-93 2.022209523432604 1106 94-95 2.526502948591851 1106 96-97 3.08243142879245 1106 98-99 3.3809436262060935 1106 100-101 3.4794348083980005 1107 1 0.12636501457437888 1107 2 0.15385646646535633 1107 3 0.1518785328724519 1107 4 0.20192773913741746 1107 5 0.08259876065069705 1107 6 0.08007522710874326 1107 7 0.11807166267652747 1107 8 0.13851042475531017 1107 9 0.2979837230278548 1107 10-11 0.2289643641401682 1107 12-13 0.24040056136518473 1107 14-15 0.3073843275974113 1107 16-17 0.27407702208637374 1107 18-19 0.22311197093413426 1107 20-21 0.28046970113919656 1107 22-23 0.26353536109834863 1107 24-25 0.29582835918149186 1107 26-27 0.3206559295101812 1107 28-29 0.26552298431372634 1107 30-31 0.2669947405248223 1107 32-33 0.27161313508814544 1107 34-35 0.251817147475613 1107 36-37 0.3025033557600594 1107 38-39 0.3696003151781966 1107 40-41 0.29388429976661 1107 42-43 0.3185626221447251 1107 44-45 0.47178569996827946 1107 46-47 0.5083551474094321 1107 48-49 0.6531553885814319 1107 50-51 0.5448341784172825 1107 52-53 0.6859412530079894 1107 54-55 0.6154168866685197 1107 56-57 0.7620734441075214 1107 58-59 0.8961495328415268 1107 60-61 0.8745964721972186 1107 62-63 1.0339942223436083 1107 64-65 1.1227477564382617 1107 66-67 1.3133795206926528 1107 68-69 1.4056279450022124 1107 70-71 1.4276984173289087 1107 72-73 1.6167734473796074 1107 74-75 1.4485697999779 1107 76-77 1.5410090513893806 1107 78-79 1.8789981544164611 1107 80-81 2.0043703094547176 1107 82-83 2.061183391109882 1107 84-85 2.359326997860361 1107 86-87 2.7072880428696457 1107 88-89 2.900347484500852 1107 90-91 3.007968476956176 1107 92-93 3.0221303722498867 1107 94-95 3.2846898192574976 1107 96-97 3.4490710851180175 1107 98-99 3.7293480043218494 1107 100-101 4.116652947738384 1108 1 0.19504195412573821 1108 2 0.16980309799041748 1108 3 0.1321330643759353 1108 4 0.13997307569583484 1108 5 0.06447117565839733 1108 6 0.05442726460819358 1108 7 0.16489256400952002 1108 8 0.1191586653344956 1108 9 0.2085633740187447 1108 10-11 0.14828039752595146 1108 12-13 0.19303277204635805 1108 14-15 0.3508052855516013 1108 16-17 0.37209047229443115 1108 18-19 0.3358293396629435 1108 20-21 0.3398504875707218 1108 22-23 0.2290867596139421 1108 24-25 0.32979527190847335 1108 26-27 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3.2874489702433856 1108 94-95 3.428054124580928 1108 96-97 3.597283228010845 1108 98-99 3.977810952150211 1108 100-101 4.196204804376748 1109 1 0.3010106899213163 1109 2 0.3029551471208336 1109 3 0.3004145846343107 1109 4 0.18118029272377356 1109 5 0.15946236766858135 1109 6 0.183054529246526 1109 7 0.23074518208216688 1109 8 0.2665196292021079 1109 9 0.2849117085533166 1109 10-11 0.35593483017094485 1109 12-13 0.3603376602644133 1109 14-15 0.38492035709696637 1109 16-17 0.48800704562368935 1109 18-19 0.5706957024589556 1109 20-21 0.6071157448259115 1109 22-23 0.45929024514214944 1109 24-25 0.4474168750757528 1109 26-27 0.4639077221308412 1109 28-29 0.5698976263461404 1109 30-31 0.601302555197293 1109 32-33 0.7165169923342205 1109 34-35 0.7491102548668991 1109 36-37 0.7561036747180196 1109 38-39 0.8577977574429596 1109 40-41 0.8608674957820597 1109 42-43 0.9592309766980804 1109 44-45 0.8817873392826812 1109 46-47 0.9784128674671564 1109 48-49 0.8979672620749355 1109 50-51 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1.2319237865097108 2316 74-75 1.2828422269596444 2316 76-77 1.349148660540692 2316 78-79 1.4279874552575365 2316 80-81 1.5044194286248178 2316 82-83 1.7340173269514274 2316 84-85 1.9377053271921802 2316 86-87 2.144528950652738 2316 88-89 2.3330488989504623 2316 90-91 2.439765774256216 2316 92-93 2.2194767509048567 2316 94-95 2.7528752972990453 2316 96-97 2.9515880243884745 2316 98-99 3.2494452977896806 2316 100-101 3.6654013162621304 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 19.0 4 46.0 5 111.0 6 200.0 7 583.0 8 1249.0 9 2271.0 10 3539.0 11 5471.0 12 7997.0 13 11478.0 14 14784.0 15 19425.0 16 24119.0 17 29775.0 18 29685.0 19 28163.0 20 29422.0 21 31855.0 22 34638.0 23 39072.0 24 45073.0 25 52474.0 26 61646.0 27 72589.0 28 85667.0 29 102040.0 30 118673.0 31 139694.0 32 165191.0 33 199377.0 34 246410.0 35 307720.0 36 384467.0 37 420896.0 38 217380.0 39 11296.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.510200380506284 18.899554355275498 14.730320754165904 23.85992451005231 2 16.394354961187556 19.996894596700184 38.85844831083388 24.750302131278374 3 19.849338985923204 23.143660483255 29.222130412353103 27.784870118468692 4 13.856075345924722 16.397186754011464 35.29398379826246 34.45275410180136 5 14.490381389163396 36.62204191469999 33.79379060871126 15.09378608742535 6 34.02738462468488 35.08056888455388 16.50837714667249 14.383669344088757 7 30.113442049054868 31.37722240693578 19.72658166975095 18.782753874258407 8 26.824325632306206 35.469327719248675 18.809617527463924 18.896729120981192 9 26.32203305214232 16.587847372337368 19.388255862881937 37.70186371263838 10-11 25.751120988663946 26.058575669409294 26.680956590577885 21.509346751348872 12-13 26.321948148055068 22.95794632635297 27.247878161955562 23.472227363636396 14-15 22.947163507272375 24.742154777434124 25.14164548875717 27.16903622653633 16-17 23.09861541810678 27.881840000624898 25.914323625173587 23.105220956094737 18-19 23.07895163149996 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27.53302087048075 23.430876084704604 44-45 23.182059238421378 26.10830269490785 27.563477196809494 23.146160869861284 46-47 23.32917868768668 26.395307174914546 27.02268130867942 23.252832828719356 48-49 23.367693451415676 26.166505745119967 27.135974574961647 23.32982622850271 50-51 23.425394269109955 26.421744412207303 26.97773033714734 23.1751309815354 52-53 23.60540791489486 26.324885829473878 26.806359927444362 23.263346328186902 54-55 23.193728915127654 26.318743242694808 27.0494788840952 23.438048958082337 56-57 23.23451290015721 26.518636956575637 27.035142480944973 23.21170766232218 58-59 23.341933551344386 26.200586245741636 26.985711321348727 23.47176888156525 60-61 23.21892450973833 26.369443494462047 27.128570938553555 23.283061057246073 62-63 23.136346794480147 26.432068749216757 27.105901547258128 23.325682909044968 64-65 22.912624524579563 26.28725633800521 27.108601497232637 23.691517640182592 66-67 22.917970302821697 26.366854548761186 27.037938847443282 23.677236300973835 68-69 23.019552605415026 26.156256129027327 27.187927970609536 23.636263294948108 70-71 22.98284020473432 26.211895470163178 26.955909986724397 23.849354338378106 72-73 23.081897151908407 26.375073102443945 26.750977585992587 23.792052159655057 74-75 22.96608013791141 26.60518818311706 26.620436957186943 23.80829472178459 76-77 23.052444575460886 26.276592384646758 26.86212493194937 23.808838107942982 78-79 23.061529312796505 26.34385340256526 26.898752555188505 23.69586472944973 80-81 23.045739738525757 26.567852941920634 26.755542076548572 23.630865243005044 82-83 23.12159085853394 26.52870309899259 26.7057636459903 23.643942396483173 84-85 23.084914870482326 26.41530869203953 26.66741725257369 23.832359184904462 86-87 23.025904110284568 26.43611017385048 26.677275306079512 23.860710409785444 88-89 22.983136684079934 26.53304570082914 26.496842585731084 23.986975029359844 90-91 22.982622524068404 26.501639937204374 26.635823564322642 23.879913974404584 92-93 22.959935336849504 26.227071581156668 26.691090817085474 24.121902264908353 94-95 22.82187903612805 26.22259338515652 26.706342912665278 24.249184666050148 96-97 22.846814635296067 26.14656216441648 26.639362634989055 24.367260565298395 98-99 22.766776283503578 26.22305186722767 26.423731167848928 24.586440681419827 100-101 22.876933406555118 25.985581829586827 26.598576522366095 24.538908241491956 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 472.0 1 325.0 2 261.5 3 358.0 4 302.5 5 275.0 6 536.0 7 1231.5 8 1967.0 9 2216.5 10 2015.5 11 1748.5 12 1755.5 13 1927.0 14 2088.5 15 2256.0 16 2408.5 17 2597.5 18 2797.5 19 3043.5 20 3428.0 21 4039.5 22 4916.5 23 5988.0 24 7277.5 25 8965.0 26 11478.0 27 14848.0 28 17838.5 29 20925.5 30 24979.5 31 30097.0 32 36816.5 33 41168.0 34 43859.5 35 48257.5 36 54301.5 37 60507.5 38 65543.0 39 70313.0 40 75512.0 41 82998.5 42 89467.5 43 94625.5 44 101342.5 45 108034.5 46 111151.0 47 114134.5 48 117092.0 49 116886.5 50 116687.0 51 114688.0 52 112699.5 53 113474.5 54 115099.0 55 110332.0 56 100571.5 57 92562.0 58 85048.5 59 74822.5 60 62158.0 61 51357.5 62 42516.5 63 33308.5 64 25498.0 65 19747.0 66 14537.5 67 11127.0 68 8446.5 69 5954.5 70 4463.5 71 3263.5 72 2393.0 73 1989.5 74 1554.0 75 1164.0 76 816.0 77 459.0 78 266.5 79 165.5 80 78.5 81 41.0 82 24.0 83 17.0 84 14.0 85 6.0 86 3.5 87 1.0 88 1.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.396163489951941E-5 2 0.0421803505452031 3 2.7169307919615526E-4 4 0.0 5 0.09706235254282647 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 9.16964142287024E-4 26-27 0.010680934175898854 28-29 0.0 30-31 0.0 32-33 4.58482071143512E-4 34-35 0.014212944205448873 36-37 0.003039566323506987 38-39 0.011428090143688282 40-41 0.006435729813458927 42-43 0.005060283600028392 44-45 0.002564103434913715 46-47 1.3584653959807763E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 1.6980817449759704E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.565971664449538E-4 68-69 2.2075062684687614E-4 70-71 0.0 72-73 3.396163489951941E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 8.490408724879852E-5 82-83 8.830025073875045E-4 84-85 0.004499916624186322 86-87 0.0014773311181290943 88-89 3.396163489951941E-5 90-91 8.999833248372643E-4 92-93 3.0565471409567467E-4 94-95 0.0 96-97 3.396163489951941E-5 98-99 0.0 100-101 6.792326979903881E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2944499.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.87715699109407 #Duplication Level Percentage of deduplicated Percentage of total 1 76.21586246692364 41.06293987342078 2 13.159168197886112 14.179571417394449 3 4.361319444460858 7.0492647719258645 4 1.9689523784182534 4.243262256001132 5 1.0567877421831118 2.8468359545931676 6 0.695901251402636 2.2495908583271182 7 0.4676038138081854 1.7635214860324544 8 0.3182430141651897 1.3716823068397517 9 0.2526025085498244 1.2248554508534766 >10 1.3560047709459897 13.250959080605709 >50 0.09452609786772227 3.5345355427675877 >100 0.0486119542423387 4.6429880932492615 >500 0.0029652697125298008 1.0784160288248927 >1k 0.0013249077437170868 1.0756967044263384 >5k 1.261816898778178E-4 0.4258801747379635 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6720 0.22822218652477044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5809 0.19728313713130824 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3985 0.13533711507458485 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 3.396163489951941E-5 0.0 0.0 0.0 6 0.0 3.396163489951941E-5 0.0 0.0 0.0 7 3.396163489951941E-5 3.396163489951941E-5 0.0 0.0 0.0 8 3.396163489951941E-5 3.396163489951941E-5 0.0 0.0 0.0 9 3.396163489951941E-5 3.396163489951941E-5 0.0 0.0 0.0 10-11 3.396163489951941E-5 3.396163489951941E-5 0.0 0.0 0.0 12-13 3.396163489951941E-5 3.396163489951941E-5 0.0 0.0 1.6980817449759704E-5 14-15 1.6980817449759703E-4 3.396163489951941E-5 0.0 0.0 3.396163489951941E-5 16-17 1.6980817449759703E-4 3.396163489951941E-5 0.0 0.0 3.396163489951941E-5 18-19 1.8678899194735673E-4 3.396163489951941E-5 0.0 3.396163489951941E-5 6.792326979903882E-5 20-21 2.0376980939711646E-4 3.396163489951941E-5 0.0 1.0188490469855823E-4 1.0188490469855823E-4 22-23 2.0376980939711646E-4 3.396163489951941E-5 0.0 2.7169307919615526E-4 1.1886572214831793E-4 24-25 2.5471226174639554E-4 5.0942452349279116E-5 0.0 4.075396187942329E-4 1.3584653959807763E-4 26-27 2.7169307919615526E-4 6.792326979903882E-5 0.0 6.622518805406285E-4 1.3584653959807763E-4 28-29 3.7357798389471347E-4 6.792326979903882E-5 0.0 0.0019358131892726065 1.3584653959807763E-4 30-31 4.415012536937523E-4 1.0188490469855823E-4 0.0 0.007420617225544991 1.5282735704783733E-4 32-33 4.415012536937523E-4 1.5282735704783733E-4 0.0 0.020852443828304916 1.6980817449759703E-4 34-35 4.924437060430314E-4 1.6980817449759703E-4 0.0 0.04128036722036584 1.6980817449759703E-4 36-37 5.7734779329183E-4 1.6980817449759703E-4 0.0 0.07996266937091845 1.6980817449759703E-4 38-39 6.452710630908688E-4 1.6980817449759703E-4 0.0 0.13448807420209685 1.6980817449759703E-4 40-41 6.452710630908688E-4 1.6980817449759703E-4 0.0 0.1960944799098251 1.6980817449759703E-4 42-43 6.792326979903882E-4 1.6980817449759703E-4 0.0 0.25861784975984026 1.6980817449759703E-4 44-45 7.811176026889464E-4 1.6980817449759703E-4 0.0 0.3374767659965244 1.8678899194735673E-4 46-47 8.830025073875045E-4 1.6980817449759703E-4 0.0 0.4341145981031068 2.0376980939711646E-4 48-49 9.16964142287024E-4 1.6980817449759703E-4 0.0 0.5399730140849088 2.0376980939711646E-4 50-51 9.16964142287024E-4 1.6980817449759703E-4 0.0 0.649991730341902 2.0376980939711646E-4 52-53 9.16964142287024E-4 1.6980817449759703E-4 0.0 0.7606047752096367 2.2075062684687616E-4 54-55 9.509257771865435E-4 1.6980817449759703E-4 0.0 0.8834270278237486 2.5471226174639554E-4 56-57 9.848874120860628E-4 1.6980817449759703E-4 0.0 1.0283243431225482 3.0565471409567467E-4 58-59 9.848874120860628E-4 1.6980817449759703E-4 0.0 1.189523243173117 3.0565471409567467E-4 60-61 9.848874120860628E-4 2.3773144429663586E-4 0.0 1.3602823434479006 3.3961634899519407E-4 62-63 0.0010188490469855824 2.3773144429663586E-4 0.0 1.5321282160394687 3.3961634899519407E-4 64-65 0.0010188490469855824 2.3773144429663586E-4 0.0 1.7158946224807683 3.3961634899519407E-4 66-67 0.0010528106818851017 2.3773144429663586E-4 0.0 1.9278152242537696 3.3961634899519407E-4 68-69 0.0010528106818851017 2.3773144429663586E-4 0.0 2.163288219829587 3.3961634899519407E-4 70-71 0.0010697914993348613 2.3773144429663586E-4 0.0 2.413619430673945 4.075396187942329E-4 72-73 0.0011037531342343808 2.3773144429663586E-4 0.0 2.6703693905143115 4.245204362439926E-4 74-75 0.0011377147691339002 2.3773144429663586E-4 0.0 2.933945638969482 4.754628885932717E-4 76-77 0.00115469558658366 2.3773144429663586E-4 0.0 3.2252855239550087 4.924437060430315E-4 78-79 0.0011716764040334195 2.3773144429663586E-4 0.0 3.555002056376993 5.094245234927912E-4 80-81 0.0014433694832295747 2.3773144429663586E-4 0.0 3.903380507176263 5.094245234927912E-4 82-83 0.001494311935578854 2.3773144429663586E-4 0.0 4.262388949699083 6.113094281913493E-4 84-85 0.0015112927530286139 2.3773144429663586E-4 0.0 4.627714256313213 6.113094281913493E-4 86-87 0.0015282735704783734 2.3773144429663586E-4 0.0 5.009952457107304 6.113094281913493E-4 88-89 0.0015282735704783734 2.3773144429663586E-4 0.0 5.420650507947192 6.452710630908688E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4925 0.0 45.099667 1 GTATCAA 7615 0.0 38.661922 1 ATCAACG 9505 0.0 30.511992 3 TCAACGC 9775 0.0 29.669207 4 TATCAAC 9830 0.0 29.659895 2 CAACGCA 9845 0.0 29.409958 5 AACGCAG 10365 0.0 27.906507 6 ACGCAGA 12030 0.0 24.083618 7 GTACATG 9360 0.0 23.933582 1 TACATGG 9155 0.0 23.84608 2 CGCAGAG 12100 0.0 23.78728 8 ACATGGG 9440 0.0 22.260921 3 GTAAGGT 1130 0.0 20.61629 4 GCAGAGT 13685 0.0 20.581043 9 GCACCGT 675 0.0 20.40571 6 GAGTACT 7535 0.0 19.950233 12-13 GTCCTAC 720 0.0 19.817616 1 TAAGGTG 1345 0.0 19.79514 5 GTACTTT 7740 0.0 19.20706 14-15 AGAGTAC 12220 0.0 18.889591 10-11 >>END_MODULE