FastQCFastQC Report
Thu 26 May 2016
SRR938532_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938532_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933957
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63010.21476115703127208No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50880.17341767449216194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34920.11902014923872435No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46550.046.30141
GTATCAA76900.040.6153641
ATCAACG97300.031.7988383
TCAACGC98650.031.363684
TATCAAC100100.031.0043182
CAACGCA99950.030.8120425
AACGCAG105050.029.263946
GTACATG94450.026.0509221
ACGCAGA118650.025.3842667
TACATGG94050.024.963832
CGCAGAG121850.024.6786448
TGGTATC23750.024.6141362
GTGGTAT25250.023.9837861
ACATGGG98850.022.7419413
GCAGAGT128500.022.6243769
GAGTACT72750.020.73171812-13
AGTACTT76250.019.90470112-13
AGAGTAC118400.019.8796710-11
GTACTTT75950.019.85941514-15
CAGAGTA125750.019.22768410-11
Produced by FastQC (version 0.11.5)