##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938532_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2933957 Sequences flagged as poor quality 0 Sequence length 101 %GC 47 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.89991298440979 31.0 31.0 34.0 27.0 34.0 2 31.071809845883905 31.0 31.0 34.0 27.0 34.0 3 31.126604445804762 31.0 31.0 34.0 27.0 34.0 4 34.78900917770778 37.0 35.0 37.0 32.0 37.0 5 34.768608060718 37.0 35.0 37.0 32.0 37.0 6 34.732555044262746 37.0 35.0 37.0 31.0 37.0 7 34.627983641205375 37.0 35.0 37.0 30.0 37.0 8 34.58386881607331 37.0 35.0 37.0 30.0 37.0 9 36.02935557678589 39.0 35.0 39.0 30.0 39.0 10-11 35.84736892871982 38.0 35.0 39.0 30.0 39.0 12-13 35.6383634797647 38.0 35.0 39.0 30.0 39.0 14-15 36.76969260285682 39.0 36.0 41.0 30.0 41.0 16-17 36.67798573735062 39.0 36.0 41.0 30.0 41.0 18-19 36.6377068580078 39.0 36.0 41.0 30.0 41.0 20-21 36.54848503233006 39.0 36.0 41.0 29.5 41.0 22-23 36.37445487442386 39.0 36.0 41.0 29.0 41.0 24-25 36.21059272511492 39.0 35.0 41.0 28.5 41.0 26-27 35.9714910613891 39.0 35.0 40.0 27.0 41.0 28-29 35.71240410135527 38.5 34.5 40.0 27.0 41.0 30-31 35.430229720476476 38.0 34.0 40.0 26.5 41.0 32-33 35.124248412638636 38.0 34.0 40.0 25.5 41.0 34-35 34.7852189040262 38.0 33.5 40.0 25.0 41.0 36-37 34.55342920840353 38.0 33.0 40.0 24.0 41.0 38-39 34.28223385686975 38.0 33.0 40.0 24.0 41.0 40-41 33.97977833349296 38.0 33.0 40.0 23.0 41.0 42-43 33.53500255797886 38.0 32.5 40.0 20.0 41.0 44-45 33.04018429717954 37.0 31.0 40.0 17.0 41.0 46-47 32.531437747724326 37.0 31.0 40.0 14.5 41.0 48-49 32.01432894210788 36.5 30.0 39.0 9.5 41.0 50-51 31.275040159075267 35.5 29.5 38.5 8.5 40.0 52-53 31.251617184573597 36.0 30.0 39.0 7.5 40.0 54-55 31.164799279607713 36.0 29.5 39.0 4.0 40.0 56-57 30.678909063766103 35.5 28.5 39.0 2.0 40.0 58-59 30.159078507285553 35.0 28.0 39.0 2.0 40.0 60-61 29.552035186609757 34.5 26.5 38.0 2.0 40.0 62-63 28.771623612752336 34.0 26.0 38.0 2.0 40.0 64-65 28.0453979046046 34.0 24.5 37.5 2.0 40.0 66-67 27.29256683039322 33.0 22.5 37.0 2.0 39.0 68-69 26.387559872213533 32.0 20.0 36.0 2.0 39.0 70-71 25.503921495781974 31.5 16.0 35.5 2.0 38.5 72-73 24.553506919153893 31.0 8.5 35.0 2.0 38.0 74-75 23.575403627251525 30.0 4.0 34.5 2.0 37.0 76-77 22.5917179426965 29.5 2.0 34.0 2.0 36.0 78-79 21.57507744660198 28.5 2.0 34.0 2.0 36.0 80-81 20.586333916959248 27.0 2.0 34.0 2.0 35.0 82-83 19.568849168546095 26.0 2.0 33.0 2.0 35.0 84-85 18.578209053506917 24.5 2.0 32.5 2.0 35.0 86-87 17.576047467635007 21.5 2.0 32.0 2.0 34.0 88-89 16.49719815252916 16.5 2.0 31.0 2.0 34.0 90-91 15.362810191151404 4.5 2.0 31.0 2.0 34.0 92-93 14.223679658563503 2.0 2.0 30.0 2.0 34.0 94-95 13.013639429616727 2.0 2.0 29.0 2.0 34.0 96-97 11.677397623755223 2.0 2.0 28.0 2.0 34.0 98-99 10.105040735089165 2.0 2.0 24.5 2.0 33.0 100-101 7.161210610789457 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6189742612220357 1101 2 0.550043216962159 1101 3 0.44500655996210625 1101 4 0.3220975702848321 1101 5 0.42510237578470367 1101 6 0.5077816267876685 1101 7 0.41816381046837137 1101 8 0.419256712920145 1101 9 0.5230859082736785 1101 10-11 0.6373313914889494 1101 12-13 0.5441951412805963 1101 14-15 0.6826263531571968 1101 16-17 0.6771062798368774 1101 18-19 0.6278945778962353 1101 20-21 0.7667829848442764 1101 22-23 0.8503536109809531 1101 24-25 0.8546511897766536 1101 26-27 0.7412120189021891 1101 28-29 0.8512214802446678 1101 30-31 0.990669756921811 1101 32-33 0.9090913343309879 1101 34-35 1.0400643121234765 1101 36-37 1.2045074699490428 1101 38-39 1.2063060692453291 1101 40-41 1.1321909223430424 1101 42-43 1.4823257817949411 1101 44-45 1.5192063680052712 1101 46-47 1.616504954879808 1101 48-49 1.2864344681139315 1101 50-51 1.5975830492670937 1101 52-53 1.6413060835223519 1101 54-55 1.837322578229685 1101 56-57 2.0864747157511943 1101 58-59 2.1018510167357043 1101 60-61 2.278915928939366 1101 62-63 2.121256756985865 1101 64-65 2.1045289359559973 1101 66-67 2.3270512969403576 1101 68-69 2.507275840257215 1101 70-71 2.8113588092754256 1101 72-73 3.000899839350584 1101 74-75 3.0872184609126556 1101 76-77 3.238433625035089 1101 78-79 3.3091737490805713 1101 80-81 3.668246806668236 1101 82-83 3.6062069883563517 1101 84-85 3.679948241484791 1101 86-87 3.882344948233822 1101 88-89 4.112897145927921 1101 90-91 4.420527869615764 1101 92-93 4.018734188278886 1101 94-95 3.809768425157406 1101 96-97 3.642010043744035 1101 98-99 3.457748632990741 1101 100-101 2.276618021637659 1102 1 0.5278463338951092 1102 2 0.4264273177215294 1102 3 0.3991842484161978 1102 4 0.33858327908164654 1102 5 0.31153961334653246 1102 6 0.3283161403758754 1102 7 0.3840935567332764 1102 8 0.5302988022499306 1102 9 0.504134151508076 1102 10-11 0.5613244680823684 1102 12-13 0.5857041779147991 1102 14-15 0.573632574617541 1102 16-17 0.7760999633762893 1102 18-19 0.7104962334338012 1102 20-21 0.7064938516053871 1102 22-23 0.6635942170402771 1102 24-25 0.8230564840346304 1102 26-27 0.7792695119935615 1102 28-29 0.6621120177982718 1102 30-31 0.4381586366156185 1102 32-33 0.6860525351762803 1102 34-35 0.8278307350265521 1102 36-37 0.9944796137667424 1102 38-39 1.0435094309872497 1102 40-41 1.2097448983143124 1102 42-43 1.241557562331721 1102 44-45 1.0830094546687192 1102 46-47 1.2960513234427538 1102 48-49 1.2638942091221566 1102 50-51 1.4869275581475065 1102 52-53 1.447880839213397 1102 54-55 1.4940828746726673 1102 56-57 1.7621539147091454 1102 58-59 2.1109160190684797 1102 60-61 2.022122092132893 1102 62-63 2.098889609547598 1102 64-65 2.3970963016837104 1102 66-67 2.5904868382200696 1102 68-69 2.562729461138691 1102 70-71 2.6415654031367204 1102 72-73 2.8179958719293303 1102 74-75 2.7232491766022626 1102 76-77 2.865998367700545 1102 78-79 3.184782674247824 1102 80-81 3.150514351629738 1102 82-83 3.434022610829608 1102 84-85 3.4944696355394633 1102 86-87 3.7924339476279307 1102 88-89 3.8927380416324304 1102 90-91 3.7855994206319465 1102 92-93 3.8334040202129014 1102 94-95 3.9950752389970443 1102 96-97 3.6531166276830813 1102 98-99 3.2204524085115 1102 100-101 2.3580956395804593 1103 1 0.5480357565851115 1103 2 0.5103834480598373 1103 3 0.4513560435208106 1103 4 0.488599099293225 1103 5 0.4795211743904204 1103 6 0.5137319286688111 1103 7 0.5371698480912457 1103 8 0.47549321380761 1103 9 0.4719541212941252 1103 10-11 0.6509378732014355 1103 12-13 0.577146341320514 1103 14-15 0.7004523099457813 1103 16-17 0.7125594936057951 1103 18-19 0.8208418930430454 1103 20-21 0.7787321497704625 1103 22-23 0.8780789169608383 1103 24-25 0.7941671463513913 1103 26-27 0.991826384452807 1103 28-29 0.8887574778780944 1103 30-31 1.0045974551404626 1103 32-33 1.0740126206939422 1103 34-35 1.1265410526625104 1103 36-37 1.1204360663264694 1103 38-39 1.2775760372395055 1103 40-41 1.378897673503161 1103 42-43 1.3260849148578728 1103 44-45 1.546298596793406 1103 46-47 1.7606028577503494 1103 48-49 1.5757970783344106 1103 50-51 1.6177925127241508 1103 52-53 1.6340851721050456 1103 54-55 1.6225160565990357 1103 56-57 1.9499510179032171 1103 58-59 2.0573845836378375 1103 60-61 2.254146171228385 1103 62-63 2.2036472315130844 1103 64-65 2.457528055617832 1103 66-67 2.512762998666105 1103 68-69 2.696934807232058 1103 70-71 2.6824854995603395 1103 72-73 2.9291897022744458 1103 74-75 2.660845650174668 1103 76-77 2.6836780168889334 1103 78-79 2.813634693430366 1103 80-81 2.965627800630614 1103 82-83 3.1974338126522603 1103 84-85 3.2745413791240594 1103 86-87 3.4874451178860326 1103 88-89 3.912496360395405 1103 90-91 3.6665302611417054 1103 92-93 3.6605541715987986 1103 94-95 3.5651391116786098 1103 96-97 3.529433682427589 1103 98-99 3.1167980281592182 1103 100-101 2.1288980796615666 1104 1 0.5115340118561349 1104 2 0.35884118783990004 1104 3 0.37452359499188503 1104 4 0.29260936717904684 1104 5 0.2822613626752499 1104 6 0.3080893017248627 1104 7 0.4036198185977611 1104 8 0.3558123665983359 1104 9 0.546233608373278 1104 10-11 0.45478171186606176 1104 12-13 0.5562763223007252 1104 14-15 0.6593813705168401 1104 16-17 0.6110282481499922 1104 18-19 0.5902346413667985 1104 20-21 0.5562068605000547 1104 22-23 0.6833073911889471 1104 24-25 0.7337975430423285 1104 26-27 0.6967596944039229 1104 28-29 0.7084250759686697 1104 30-31 0.5086256944835625 1104 32-33 0.755911909033891 1104 34-35 0.8777584739612934 1104 36-37 1.0705489072708616 1104 38-39 1.0091461145699157 1104 40-41 0.8924338161810752 1104 42-43 0.9824550718037699 1104 44-45 0.9479786623409154 1104 46-47 1.1410793815680904 1104 48-49 1.2534715645173975 1104 50-51 1.1347102000681417 1104 52-53 1.4120030700822355 1104 54-55 1.3992721505897627 1104 56-57 1.552116336952139 1104 58-59 1.6881917919313736 1104 60-61 1.6418105532977023 1104 62-63 1.6849352366764947 1104 64-65 1.6809181608812942 1104 66-67 2.015619892724846 1104 68-69 2.381471117063846 1104 70-71 2.32810098332347 1104 72-73 2.6040435271754454 1104 74-75 2.5242879982711663 1104 76-77 2.625812702332876 1104 78-79 2.773518185728971 1104 80-81 2.9381945160424436 1104 82-83 3.0912453872369454 1104 84-85 3.1917393390737985 1104 86-87 3.071651479975145 1104 88-89 3.1930846744070074 1104 90-91 2.8084848455300175 1104 92-93 2.7903354488186682 1104 94-95 2.805849148495943 1104 96-97 2.856742350418928 1104 98-99 2.6054933414253902 1104 100-101 1.693785970619266 1105 1 0.5230721017114632 1105 2 0.41134061753859186 1105 3 0.34937028731397746 1105 4 0.3842651504989334 1105 5 0.37122894772448234 1105 6 0.4316554759578395 1105 7 0.43890102708830625 1105 8 0.4686668651954591 1105 9 0.4787771847362734 1105 10-11 0.5143524131996102 1105 12-13 0.633751639056058 1105 14-15 0.6899974910648297 1105 16-17 0.7032509644010432 1105 18-19 0.6863980119009625 1105 20-21 0.7147483015434446 1105 22-23 0.7201748155316849 1105 24-25 0.8654535168498185 1105 26-27 0.6495882262880599 1105 28-29 0.7917444131089724 1105 30-31 0.8329546554294183 1105 32-33 0.9348307617781515 1105 34-35 0.9627667044197068 1105 36-37 1.0395840434067765 1105 38-39 1.2374219322611992 1105 40-41 1.3655737279342048 1105 42-43 1.4667074381476795 1105 44-45 1.5610217901288195 1105 46-47 1.6487400032731827 1105 48-49 1.7059807392832766 1105 50-51 1.5425612396890926 1105 52-53 1.6805412890974623 1105 54-55 1.8548450978186501 1105 56-57 1.9871231271384033 1105 58-59 2.038956715822284 1105 60-61 2.3284317259487004 1105 62-63 2.406876354169718 1105 64-65 2.584403491709484 1105 66-67 2.7716429598447796 1105 68-69 3.030797952446779 1105 70-71 2.84452293149133 1105 72-73 3.063451315145084 1105 74-75 3.2613875155012373 1105 76-77 3.2762845540721877 1105 78-79 3.71444977930647 1105 80-81 3.7886859043765178 1105 82-83 3.8516616328563096 1105 84-85 3.847557039646791 1105 86-87 3.926892688391355 1105 88-89 3.987127151284831 1105 90-91 3.6907819727446096 1105 92-93 3.780668493168365 1105 94-95 3.6928621216311495 1105 96-97 3.5189480064141065 1105 98-99 3.172981416204321 1105 100-101 2.3355717617305567 1106 1 0.364228542493354 1106 2 0.18526425461139695 1106 3 0.044043949698849616 1106 4 0.2204725449736742 1106 5 0.18398831143244365 1106 6 -0.11722353373911432 1106 7 0.19341942856186733 1106 8 -0.18921313682497498 1106 9 0.10279417632224863 1106 10-11 0.22595225790698947 1106 12-13 0.3559477249843397 1106 14-15 0.5473139004620293 1106 16-17 -0.12655066603558396 1106 18-19 0.38282755002546054 1106 20-21 0.32098756090454117 1106 22-23 0.2489050265380186 1106 24-25 0.4892556673445867 1106 26-27 0.35885052744113466 1106 28-29 0.4038341566609631 1106 30-31 0.13628434125858035 1106 32-33 0.37630564756808127 1106 34-35 0.3808152474075115 1106 36-37 0.5492943057892461 1106 38-39 0.6559756972352346 1106 40-41 0.7603715270191671 1106 42-43 0.3035701715651129 1106 44-45 0.8333547877045291 1106 46-47 0.927143479146082 1106 48-49 0.8449330939393747 1106 50-51 0.7953435280853398 1106 52-53 1.045396955975761 1106 54-55 0.8253492959970394 1106 56-57 1.225872339746367 1106 58-59 1.3770689138357781 1106 60-61 1.8201286330750577 1106 62-63 1.8182349245172063 1106 64-65 1.7029363728157243 1106 66-67 1.906117570882742 1106 68-69 1.8584252370162062 1106 70-71 1.74135798359114 1106 72-73 1.7171632640924095 1106 74-75 1.984051789658853 1106 76-77 2.1863265573741977 1106 78-79 2.5488319305305893 1106 80-81 2.4217138205810187 1106 82-83 2.494304282470992 1106 84-85 2.51673171462744 1106 86-87 2.2900360253389707 1106 88-89 2.296765486636712 1106 90-91 2.410483149965067 1106 92-93 2.4698320534918228 1106 94-95 2.3420503462101934 1106 96-97 2.212141111294839 1106 98-99 2.2013145619150514 1106 100-101 1.4691920481657155 1107 1 0.29582300787731697 1107 2 0.1791727383000108 1107 3 0.15884990076663286 1107 4 0.23548880071039235 1107 5 0.17197439737066134 1107 6 0.17243303648449881 1107 7 0.2503240348664164 1107 8 0.2223387219417532 1107 9 0.3144660854477692 1107 10-11 0.2753904204955404 1107 12-13 0.31349409400233696 1107 14-15 0.21038446989136617 1107 16-17 0.29552497954907864 1107 18-19 0.3140598505012875 1107 20-21 0.3193792022467363 1107 22-23 0.3641640897554552 1107 24-25 0.38988822706335924 1107 26-27 0.34071737981341244 1107 28-29 0.378222763829946 1107 30-31 0.5517877493981871 1107 32-33 0.6930549679539908 1107 34-35 0.5346946236480292 1107 36-37 0.538717358143245 1107 38-39 0.5664645559816108 1107 40-41 0.5958409295520966 1107 42-43 0.84815261762148 1107 44-45 0.5901035061063169 1107 46-47 0.5472766648850325 1107 48-49 0.4626317086396483 1107 50-51 0.8702000371577654 1107 52-53 0.9171215018999987 1107 54-55 1.1010173143129514 1107 56-57 1.3711853520012909 1107 58-59 1.362125199351219 1107 60-61 1.3200938782363387 1107 62-63 1.4735031950601325 1107 64-65 1.489016442767145 1107 66-67 1.6665988073841156 1107 68-69 1.611059350633564 1107 70-71 1.8270286610311608 1107 72-73 1.8270812340113913 1107 74-75 1.877845549898538 1107 76-77 1.9737421442589103 1107 78-79 2.280018410919986 1107 80-81 2.533363036204367 1107 82-83 2.505316694170965 1107 84-85 2.470249987010966 1107 86-87 2.404883877100165 1107 88-89 2.568181613141725 1107 90-91 2.579705434118029 1107 92-93 2.344176421324045 1107 94-95 2.4011022818489973 1107 96-97 2.3257013016950534 1107 98-99 2.007746783916401 1107 100-101 1.3717125210394014 1108 1 0.4054395806469273 1108 2 0.23871433994693092 1108 3 0.2194885961225772 1108 4 0.09793571651562161 1108 5 0.09373946668841882 1108 6 0.26627209521839035 1108 7 0.23558317365090886 1108 8 0.3197932447517786 1108 9 0.35236984890044454 1108 10-11 0.34917439865379407 1108 12-13 0.28683630420964334 1108 14-15 0.29764859779649555 1108 16-17 0.4939325758088273 1108 18-19 0.3756424005451251 1108 20-21 0.4260799600167502 1108 22-23 0.41448662431665184 1108 24-25 0.5126393986397844 1108 26-27 0.4647948931165118 1108 28-29 0.5533236817988367 1108 30-31 0.5800460017163047 1108 32-33 0.6186452129166398 1108 34-35 0.6163752955065007 1108 36-37 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-0.6787229665413861 2315 86-87 -0.5611766372011004 2315 88-89 -0.7589419478547228 2315 90-91 -0.769227355606759 2315 92-93 -0.651272929896459 2315 94-95 -0.529966436447264 2315 96-97 -0.48666393018871723 2315 98-99 -0.7504475981945031 2315 100-101 -0.44853933049202066 2316 1 -1.1245503618595265 2316 2 -0.9881538225780275 2316 3 -0.8417595113864209 2316 4 -0.7151483110790551 2316 5 -0.6034778805591685 2316 6 -0.5581959095045335 2316 7 -0.6468259758446493 2316 8 -0.6746957140769183 2316 9 -1.0327960639513165 2316 10-11 -0.8633918111903043 2316 12-13 -1.01165595661071 2316 14-15 -1.1514492601442896 2316 16-17 -1.1211138262610518 2316 18-19 -1.0176868246858604 2316 20-21 -0.9511724870481828 2316 22-23 -1.2108489237332307 2316 24-25 -1.0711185645368246 2316 26-27 -1.0549676939619346 2316 28-29 -1.1921737107691186 2316 30-31 -1.0138071358120655 2316 32-33 -1.2485576446204973 2316 34-35 -0.8975954339660106 2316 36-37 -1.193183564418888 2316 38-39 -1.3403057127663303 2316 40-41 -1.253282909486515 2316 42-43 -0.9881324550656245 2316 44-45 -0.9893912763059376 2316 46-47 -0.7664948708517301 2316 48-49 -0.42008149140089657 2316 50-51 -0.2234049339848383 2316 52-53 -0.34112344752625035 2316 54-55 -0.5619545392693333 2316 56-57 -0.5254801921701073 2316 58-59 -0.2019242396127474 2316 60-61 -0.4603932041001997 2316 62-63 -0.437770909618898 2316 64-65 -0.3376061019759824 2316 66-67 -0.22090792103792722 2316 68-69 -0.14634015026916813 2316 70-71 -0.0034868968137224954 2316 72-73 0.21973894610808387 2316 74-75 0.1603900028271923 2316 76-77 0.22674345880767888 2316 78-79 0.32534726878205333 2316 80-81 0.16050129396676382 2316 82-83 0.26211030961134085 2316 84-85 0.557753632097711 2316 86-87 0.8744649606567378 2316 88-89 0.5419283733725031 2316 90-91 0.45134119790552774 2316 92-93 0.2696460102670848 2316 94-95 0.30391333647259877 2316 96-97 0.3063868258791853 2316 98-99 0.26224794963882303 2316 100-101 0.336021554667707 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11778.0 3 4957.0 4 5619.0 5 6484.0 6 7706.0 7 9676.0 8 12207.0 9 15363.0 10 19347.0 11 23704.0 12 28407.0 13 32973.0 14 36885.0 15 41283.0 16 46219.0 17 52377.0 18 53813.0 19 56157.0 20 61341.0 21 67057.0 22 73641.0 23 81394.0 24 89036.0 25 96959.0 26 106103.0 27 114755.0 28 125080.0 29 135877.0 30 149128.0 31 163134.0 32 179699.0 33 197342.0 34 216767.0 35 227297.0 36 213502.0 37 139427.0 38 31123.0 39 340.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.89496859972389 17.972727692631413 14.022194888709071 24.110108818935625 2 15.892361019958056 19.230909539085083 39.79515195692204 25.081577484034817 3 19.74572003427314 22.82351753838936 28.6787281842021 28.752034243135398 4 13.333988023266045 15.64899890407633 35.57666930588545 35.44034376677217 5 14.697331053762605 36.40614965702419 33.5989763076752 15.297542981538001 6 34.13403483237376 34.49445792796107 16.794099823795428 14.577407415869734 7 30.278957137966728 31.283440146314916 19.687014551024053 18.7505881646943 8 26.943570701568646 34.90879829240807 19.046844552191857 19.100786453831425 9 26.635322983257648 16.595160834420724 19.42062716235103 37.348889019970606 10-11 25.8413459899211 25.78708155179586 26.700217535102254 21.671354923180786 12-13 26.429192054343876 22.89344428990561 27.13605522076561 23.541308434984902 14-15 23.180434932321813 24.617969920351733 25.103302051790127 27.09829309553633 16-17 23.268954170713588 27.71730737302948 25.801938363596754 23.211800092660177 18-19 23.28884743212691 26.471267583113395 27.61724362649162 22.622641358268076 20-21 23.656723465960486 25.778352169259783 27.84256445453209 22.722359910247636 22-23 23.4585272840871 25.559392188668017 27.674611082745976 23.307469444498903 24-25 23.316781591166567 25.746026090092055 27.773817672827896 23.163374645913482 26-27 23.002789386488402 26.096038634758806 27.63891270867124 23.262259270081554 28-29 22.992019218246153 26.305366502946082 27.687530301263603 23.015083977544165 30-31 23.133888427276634 25.926786516133717 27.663770726948943 23.2755543296407 32-33 22.966711328853478 25.950281780896177 27.611783316798093 23.471223573452253 34-35 23.514292143412383 26.051710147694575 27.371022951982216 23.062974756910823 36-37 23.48650181058928 25.830227038792398 27.39369926522908 23.289571885389236 38-39 23.114919066182875 26.101786666339414 27.40943607448829 23.373858192989417 40-41 23.487224710749537 25.87323958305459 27.417192717346268 23.222342988849608 42-43 23.247562222532427 25.88549728001456 27.371931781943704 23.49500871550931 44-45 23.26114949307122 26.09335466095809 27.38381799139112 23.261677854579574 46-47 23.414198200999163 26.33246521341381 26.859746218530656 23.393590367056376 48-49 23.442619066814867 26.174816379411585 26.927485655427198 23.455078898346354 50-51 23.485443472486686 26.430564105920446 26.812722806601315 23.271269614991557 52-53 23.55519870087018 26.259418282170778 26.778906593002045 23.406476423957002 54-55 23.24771478109228 26.287248092462377 26.935152157325387 23.52988496911995 56-57 23.287601191269523 26.454105206537733 26.962426333914742 23.295867268278002 58-59 23.408607712293435 26.099687353273758 26.932406442448375 23.559298491984435 60-61 23.268567427799418 26.251794010981623 27.079139638264664 23.40049892295429 62-63 23.2162235543421 26.32632444961142 27.091087990758073 23.366364005288407 64-65 23.043717827908424 26.182907826185364 27.04726688798867 23.726107457917543 66-67 23.079229058556844 26.24385127826435 26.922620052923783 23.75429961025502 68-69 23.128036088712463 26.011867663131795 27.147317760540574 23.712778487615168 70-71 23.136805340374565 26.049819043440824 26.91995130859016 23.89342430759445 72-73 23.23387989094754 26.097580679798504 26.7370605876534 23.931478841600555 74-75 23.080894281091034 26.351854143295363 26.686253395550004 23.8809981800636 76-77 23.303665787882874 26.026765042277233 26.714800090463203 23.95476907937669 78-79 23.3951421273892 26.009153719009394 26.70669274093789 23.889011412663518 80-81 23.338411266945005 26.24444029331799 26.497814295570503 23.919334144166502 82-83 23.409554239026928 26.14832882884649 26.46274240840427 23.979374523722313 84-85 23.47306442439729 25.98394277250068 26.426422383107717 24.11657041999431 86-87 23.41873510397077 25.95244688982413 26.442617321275343 24.186200684929755 88-89 23.46353955829026 25.97076005948551 26.317429339651184 24.248271042573048 90-91 23.45212814375381 25.970898362457582 26.291465708362466 24.285507785426137 92-93 23.52919621255828 25.59284234530963 26.39379201794356 24.48416942418853 94-95 23.390978841836468 25.58458995564642 26.365247764551192 24.659183437965922 96-97 23.492489292332614 25.438959818804857 26.262471824619247 24.806079064243278 98-99 23.456253769874984 25.44301517051382 25.93070639553166 25.17002466407953 100-101 24.449478599787437 24.457514972649886 26.069055135830908 25.023951291731766 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 357.0 1 268.0 2 240.0 3 322.5 4 281.5 5 247.0 6 438.0 7 951.0 8 1601.5 9 1929.5 10 1762.5 11 1496.0 12 1502.0 13 1678.0 14 1891.0 15 2089.0 16 2261.5 17 2474.5 18 2722.0 19 2986.5 20 3331.0 21 3849.5 22 4662.5 23 5745.0 24 7175.5 25 8999.0 26 11372.5 27 14479.0 28 17579.0 29 20730.5 30 24668.5 31 29545.0 32 35202.5 33 39415.5 34 42749.0 35 47039.0 36 52398.5 37 58687.0 38 64475.5 39 69224.0 40 73832.5 41 80509.0 42 87049.0 43 92371.0 44 98233.0 45 103997.5 46 108188.5 47 111362.5 48 114366.5 49 115066.0 50 114352.0 51 113411.5 52 112499.0 53 112237.0 54 111849.0 55 107867.5 56 101173.5 57 93783.5 58 85871.0 59 77165.5 60 65869.0 61 55252.5 62 46154.5 63 37301.5 64 29823.5 65 23438.0 66 17934.5 67 13894.0 68 10632.5 69 7859.5 70 5870.5 71 4305.5 72 3191.5 73 2486.5 74 1902.0 75 1453.0 76 1036.0 77 653.0 78 395.0 79 239.0 80 150.0 81 90.5 82 55.0 83 40.0 84 27.5 85 14.0 86 11.5 87 11.0 88 8.5 89 8.0 90 6.5 91 2.5 92 1.0 93 1.5 94 0.5 95 0.5 96 1.0 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.38214602327164304 2 0.029175615048209636 3 0.035719678236593104 4 0.04332033496060099 5 0.06223676761452195 6 0.05351135003001066 7 0.037662446995644444 8 0.03994605237909076 9 0.03735569403368897 10-11 0.037389777696128466 12-13 0.029226740541868884 14-15 0.027641850238432263 16-17 0.022427049885189183 18-19 0.026602298534027593 20-21 0.034015495114618245 22-23 0.03321452904729006 24-25 0.04207628128155934 26-27 0.03592418021123009 28-29 0.031050216482381984 30-31 0.03408366243949724 32-33 0.028187188837464218 34-35 0.02989137195943908 36-37 0.031834140718490424 38-39 0.015303564435334259 40-41 0.013071084545547191 42-43 0.013411921169942165 44-45 0.012253076646999258 46-47 0.020671741269555077 48-49 0.018081382924153285 50-51 0.01223603481577951 52-53 0.01025918239428867 54-55 0.009083296040126015 56-57 0.009543425483059226 58-59 0.004584252598112379 60-61 0.008094869829380595 62-63 0.012627996933833728 64-65 0.007583614892788135 66-67 0.003936663011761932 68-69 0.0063566030449662345 70-71 0.01295179172700895 72-73 0.011009022967957608 74-75 0.011520277904550066 76-77 0.0057090134586157875 78-79 0.009747927457696209 80-81 0.011895198191384536 82-83 0.013650506807018643 84-85 0.008946961390368026 86-87 0.0027096511639400304 88-89 0.016002279515343956 90-91 0.024540236956438012 92-93 0.010719311837221883 94-95 0.020364988307599603 96-97 0.018575596029525996 98-99 0.011537319735769816 100-101 0.120332370242645 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2933957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.327746073970154 #Duplication Level Percentage of deduplicated Percentage of total 1 78.65798467122495 45.092849719222215 2 11.97364342963906 13.728439802292183 3 3.8429431597920574 6.609218089237788 4 1.744964188861762 4.001394557089536 5 0.9611623373877313 2.7550635206813743 6 0.5926931384840479 2.0386657045678755 7 0.4204192412026476 1.687118125299362 8 0.2786917414787131 1.278141551072336 9 0.2206555465206754 1.138471662667535 >10 1.185886010980981 12.239734984582306 >50 0.07568386571980416 3.023832784897787 >100 0.04174069710892815 4.21023045105545 >500 0.002334693090700637 0.9245441385231473 >1k 0.001077550657076749 0.8814399276537177 >5k 1.1972785078630542E-4 0.39085498115745954 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6301 0.21476115703127208 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5088 0.17341767449216194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3492 0.11902014923872435 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 3.408366243949724E-5 0.0 0.0 0.0 0.0 3 3.408366243949724E-5 0.0 0.0 0.0 0.0 4 3.408366243949724E-5 0.0 0.0 0.0 0.0 5 3.408366243949724E-5 3.408366243949724E-5 0.0 0.0 3.408366243949724E-5 6 6.816732487899447E-5 3.408366243949724E-5 0.0 0.0 3.408366243949724E-5 7 1.0225098731849171E-4 3.408366243949724E-5 0.0 0.0 3.408366243949724E-5 8 1.0225098731849171E-4 3.408366243949724E-5 0.0 0.0 3.408366243949724E-5 9 1.0225098731849171E-4 3.408366243949724E-5 0.0 0.0 3.408366243949724E-5 10-11 1.0225098731849171E-4 6.816732487899447E-5 0.0 0.0 6.816732487899447E-5 12-13 2.3858563707648066E-4 6.816732487899447E-5 0.0 0.0 1.0225098731849171E-4 14-15 2.3858563707648066E-4 6.816732487899447E-5 0.0 0.0 1.0225098731849171E-4 16-17 2.3858563707648066E-4 6.816732487899447E-5 0.0 5.1125493659245856E-5 1.0225098731849171E-4 18-19 2.556274682962293E-4 6.816732487899447E-5 0.0 6.816732487899447E-5 1.0225098731849171E-4 20-21 2.726692995159779E-4 6.816732487899447E-5 0.0 1.0225098731849171E-4 1.1929281853824033E-4 22-23 2.726692995159779E-4 6.816732487899447E-5 0.0 2.3858563707648066E-4 1.5337648097773755E-4 24-25 3.4083662439497237E-4 6.816732487899447E-5 0.0 3.7492028683446964E-4 2.0450197463698342E-4 26-27 3.4083662439497237E-4 6.816732487899447E-5 0.0 6.646314175701961E-4 2.0450197463698342E-4 28-29 4.430876117134641E-4 6.816732487899447E-5 0.0 0.002300647214666064 2.0450197463698342E-4 30-31 4.430876117134641E-4 6.816732487899447E-5 0.0 0.008282329972797828 2.0450197463698342E-4 32-33 4.601294429332127E-4 6.816732487899447E-5 0.0 0.02068878310077482 2.3858563707648066E-4 34-35 5.453385990319558E-4 6.816732487899447E-5 0.0 0.04253641072449256 2.3858563707648066E-4 36-37 6.135059239109503E-4 6.816732487899447E-5 0.0 0.08043744335721348 2.3858563707648066E-4 38-39 7.498405736689393E-4 6.816732487899447E-5 0.0 0.13590860397749524 3.0675296195547516E-4 40-41 7.498405736689393E-4 6.816732487899447E-5 0.0 0.19582768254613137 3.0675296195547516E-4 42-43 8.350497297676823E-4 6.816732487899447E-5 0.0 0.2587461234094433 3.0675296195547516E-4 44-45 9.202588858664254E-4 6.816732487899447E-5 0.0 0.3355706985480701 3.0675296195547516E-4 46-47 9.543425483059226E-4 6.816732487899447E-5 0.0 0.4307493259103661 3.5787845561472103E-4 48-49 9.8842621074542E-4 6.816732487899447E-5 0.0 0.5361019265108521 3.9196211805421824E-4 50-51 0.0010225098731849172 6.816732487899447E-5 0.0 0.6459535705533517 4.430876117134641E-4 52-53 0.0010225098731849172 6.816732487899447E-5 0.0 0.7546974955665676 4.771712741529613E-4 54-55 0.0010225098731849172 6.816732487899447E-5 0.0 0.8759160410326395 4.771712741529613E-4 56-57 0.0010565935356244144 6.816732487899447E-5 0.0 1.0209931502063596 4.771712741529613E-4 58-59 0.0010565935356244144 6.816732487899447E-5 0.0 1.1820214134017641 4.771712741529613E-4 60-61 0.0010565935356244144 6.816732487899447E-5 0.0 1.3536326537846328 4.771712741529613E-4 62-63 0.0010906771980639116 6.816732487899447E-5 0.0 1.532605965254433 5.112549365924586E-4 64-65 0.0011247608605034088 6.816732487899447E-5 0.0 1.7138799239388989 5.112549365924586E-4 66-67 0.0011247608605034088 6.816732487899447E-5 0.0 1.927959407721381 5.282967678122072E-4 68-69 0.0011247608605034088 6.816732487899447E-5 0.0 2.1629833020729343 5.453385990319558E-4 70-71 0.0011247608605034088 6.816732487899447E-5 0.0 2.4122371254929775 6.135059239109503E-4 72-73 0.0011418026917231574 1.0225098731849171E-4 0.0 2.6665182891228465 6.135059239109503E-4 74-75 0.001158844522942906 1.0225098731849171E-4 0.0 2.9248213249205763 6.135059239109503E-4 76-77 0.001158844522942906 1.0225098731849171E-4 0.0 3.2075793885186457 6.135059239109503E-4 78-79 0.001158844522942906 1.1929281853824033E-4 0.0 3.515320776684866 6.135059239109503E-4 80-81 0.0012270118478219006 1.3633464975798895E-4 0.0 3.843478278652346 6.135059239109503E-4 82-83 0.0012610955102613979 1.3633464975798895E-4 1.704183121974862E-5 4.1790660190316355 6.475895863504475E-4 84-85 0.0012781373414811465 1.7041831219748619E-4 3.408366243949724E-5 4.507632524948389 6.475895863504475E-4 86-87 0.0013122210039206437 1.7041831219748619E-4 3.408366243949724E-5 4.853087485603913 6.475895863504475E-4 88-89 0.0013463046663601409 1.7041831219748619E-4 3.408366243949724E-5 5.215771737622603 6.475895863504475E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4655 0.0 46.3014 1 GTATCAA 7690 0.0 40.615364 1 ATCAACG 9730 0.0 31.798838 3 TCAACGC 9865 0.0 31.36368 4 TATCAAC 10010 0.0 31.004318 2 CAACGCA 9995 0.0 30.812042 5 AACGCAG 10505 0.0 29.26394 6 GTACATG 9445 0.0 26.050922 1 ACGCAGA 11865 0.0 25.384266 7 TACATGG 9405 0.0 24.96383 2 CGCAGAG 12185 0.0 24.678644 8 TGGTATC 2375 0.0 24.614136 2 GTGGTAT 2525 0.0 23.983786 1 ACATGGG 9885 0.0 22.741941 3 GCAGAGT 12850 0.0 22.624376 9 GAGTACT 7275 0.0 20.731718 12-13 AGTACTT 7625 0.0 19.904701 12-13 AGAGTAC 11840 0.0 19.87967 10-11 GTACTTT 7595 0.0 19.859415 14-15 CAGAGTA 12575 0.0 19.227684 10-11 >>END_MODULE