FastQCFastQC Report
Thu 26 May 2016
SRR938532_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938532_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2933957
Sequences flagged as poor quality0
Sequence length101
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64830.2209643835952606No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56830.1936974536436628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38570.13146068602914085No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43250.048.7773631
GTATCAA70250.039.9049871
ATCAACG91000.030.5348153
TCAACGC92650.030.0417774
TATCAAC93600.029.7906572
CAACGCA94300.029.4657575
AACGCAG99400.027.9061496
GTGGTAT23800.024.7551861
GTACATG95200.023.8568131
ACGCAGA116400.023.626487
TACATGG93750.023.3586372
CGCAGAG119350.022.9629048
ACATGGG96350.022.0361963
TGGTATC23100.022.0033912
TAAGGTG12600.021.4870975
GCAGAGT133050.020.0986549
GCACCGT7300.019.5196426
TAGACCG1951.0339567E-519.4862735
GTACTTT75650.019.43234314-15
GTAAGGT12250.019.3868544
Produced by FastQC (version 0.11.5)