FastQCFastQC Report
Thu 26 May 2016
SRR938531_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938531_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543884
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79370.22396331256892155No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62930.1775735323165205No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA50050.1412292275932282No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA49770.14043913401228708No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA44450.1254273559744055No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA43690.1232828162547081No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42650.12034818295406961No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA39100.11033092505285161No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC38230.10787599142635595No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48350.053.115131
GTATCAA91100.042.8071671
TATCAAC116900.033.1145322
ATCAACG116900.032.992493
TCAACGC118100.032.6572574
CAACGCA119800.031.9953585
AACGCAG125850.030.4721986
ACGCAGA141850.026.8382477
CGCAGAG142850.026.6496168
TGGTATC24700.023.8744562
GTACATG108800.023.7351471
TACATGG105100.023.3483262
GTAAGGT20600.023.3164774
TAAGGTG21700.022.7919965
GCAGAGT161050.022.7815369
GTGGTAT28550.022.321311
ACATGGG106300.022.011043
GAGTACT99350.020.92665312-13
AGAGTAC142200.020.72093410-11
CAGAGTA151850.019.81098710-11
Produced by FastQC (version 0.11.5)