##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938531_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3543884 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.935422265514333 31.0 31.0 34.0 27.0 34.0 2 30.989632843512936 31.0 31.0 34.0 27.0 34.0 3 31.030196247958454 31.0 31.0 34.0 27.0 34.0 4 34.682861515783244 37.0 35.0 37.0 32.0 37.0 5 34.645716112604134 37.0 35.0 37.0 32.0 37.0 6 34.6535481974015 37.0 35.0 37.0 30.0 37.0 7 34.54692930129767 37.0 35.0 37.0 30.0 37.0 8 34.50662775643898 37.0 35.0 37.0 30.0 37.0 9 35.97802947274798 39.0 35.0 39.0 30.0 39.0 10-11 35.802237883632756 38.0 35.0 39.0 30.0 39.0 12-13 35.64878520290168 38.0 35.0 39.0 30.0 39.0 14-15 36.577241100442336 39.0 36.0 41.0 30.0 41.0 16-17 36.382792156853895 39.0 36.0 40.5 29.0 41.0 18-19 36.52493591776706 39.0 36.0 41.0 30.0 41.0 20-21 36.49181477158959 39.0 36.0 41.0 29.0 41.0 22-23 36.3968156124749 39.0 36.0 41.0 29.0 41.0 24-25 36.24164842867317 39.0 35.5 41.0 28.0 41.0 26-27 36.01434372569757 39.0 35.0 40.5 27.0 41.0 28-29 35.78836891952446 38.5 35.0 40.0 27.0 41.0 30-31 35.51543842857159 38.0 34.0 40.0 26.5 41.0 32-33 35.22496884773881 38.0 34.0 40.0 25.5 41.0 34-35 34.95439735047761 38.0 34.0 40.0 25.0 41.0 36-37 34.653425591808315 38.0 33.5 40.0 24.5 41.0 38-39 34.48382692548627 38.0 33.0 40.0 24.0 41.0 40-41 34.36211442022369 38.0 33.0 40.0 24.0 41.0 42-43 34.048938255315356 38.0 33.0 40.0 22.5 41.0 44-45 33.68661389594016 38.0 32.5 40.0 21.0 41.0 46-47 33.28634853736747 37.5 32.0 40.0 18.5 41.0 48-49 32.904182811852756 37.0 31.0 40.0 16.0 41.0 50-51 32.204166671369606 36.5 30.5 39.5 14.5 40.5 52-53 32.21724864583604 36.5 31.0 39.0 13.5 40.0 54-55 32.29133431003949 37.0 31.0 39.5 10.5 41.0 56-57 31.931255368403708 36.0 30.5 39.0 8.0 41.0 58-59 31.515714961324917 36.0 30.0 39.0 7.0 40.5 60-61 31.002217200111517 35.0 29.0 39.0 2.0 40.0 62-63 30.398756279833087 35.0 28.0 38.5 2.0 40.0 64-65 29.83655249438187 34.5 28.0 38.0 2.0 40.0 66-67 29.257029998724562 34.0 26.5 38.0 2.0 40.0 68-69 28.61732819132906 34.0 26.0 37.0 2.0 39.0 70-71 27.93186091869824 33.5 25.5 36.0 2.0 39.0 72-73 27.266000100454754 33.0 24.5 36.0 2.0 39.0 74-75 26.556095233365426 32.0 22.0 35.0 2.0 38.0 76-77 25.77550887670138 31.5 19.5 35.0 2.0 37.0 78-79 24.957003530589603 31.0 17.5 35.0 2.0 36.5 80-81 24.229611917320092 31.0 10.5 34.0 2.0 36.0 82-83 23.456232060643067 30.0 6.0 34.0 2.0 35.5 84-85 22.716109500198087 29.5 2.0 34.0 2.0 35.0 86-87 21.920171060903797 29.0 2.0 34.0 2.0 35.0 88-89 21.045500078445006 28.5 2.0 34.0 2.0 35.0 90-91 20.092965655760743 26.5 2.0 33.0 2.0 35.0 92-93 19.11610128886837 25.5 2.0 33.0 2.0 35.0 94-95 18.06854386317385 24.0 2.0 32.0 2.0 34.0 96-97 16.802410575515452 18.5 2.0 32.0 2.0 34.0 98-99 15.286545073145735 2.0 2.0 31.5 2.0 34.0 100-101 11.999763254101996 2.0 2.0 27.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6091006503258534 1101 2 0.49977162140974585 1101 3 0.44109994140853814 1101 4 0.3701433795125624 1101 5 0.452330869886417 1101 6 0.6235753807828459 1101 7 0.5341265157993789 1101 8 0.6275982724672176 1101 9 0.8094038089947801 1101 10-11 0.8652354539819527 1101 12-13 0.9733661034903633 1101 14-15 1.049730495962514 1101 16-17 0.9024209307888427 1101 18-19 0.9489047547078329 1101 20-21 1.0769661704717421 1101 22-23 0.8931078879066305 1101 24-25 1.009754110526707 1101 26-27 1.0853600796443033 1101 28-29 1.0079332390872793 1101 30-31 0.9636794812608613 1101 32-33 1.0975092038870713 1101 34-35 1.1039221255397536 1101 36-37 1.1608478307575254 1101 38-39 1.0696012069889704 1101 40-41 1.0484300241976783 1101 42-43 1.2923849962898828 1101 44-45 1.2581240391899513 1101 46-47 1.1631072405114082 1101 48-49 1.108112157821438 1101 50-51 1.1065262842410775 1101 52-53 1.0334201140735075 1101 54-55 1.0660284346491906 1101 56-57 1.0636187444832643 1101 58-59 1.1653608623145075 1101 60-61 1.1292423152494528 1101 62-63 1.09065993268851 1101 64-65 1.101978995321641 1101 66-67 0.99091437418749 1101 68-69 1.2797414413690582 1101 70-71 1.2768855761873645 1101 72-73 1.4407019168735218 1101 74-75 1.3498841879428234 1101 76-77 1.3689721628011249 1101 78-79 1.5948515561994014 1101 80-81 1.6077968990218956 1101 82-83 1.6323735190678903 1101 84-85 1.52603622894679 1101 86-87 1.69551362421576 1101 88-89 1.6878456162363342 1101 90-91 1.55859049919286 1101 92-93 1.5883228209906974 1101 94-95 1.427229251334115 1101 96-97 1.336280617445663 1101 98-99 1.3531411099192088 1101 100-101 1.3018828054383889 1102 1 0.449192079015571 1102 2 0.4522515982964954 1102 3 0.31651733218605926 1102 4 0.2271038957703979 1102 5 0.2557368803493034 1102 6 0.3864146168855598 1102 7 0.4710564416172929 1102 8 0.48059587949963145 1102 9 0.62736668948925 1102 10-11 0.7681891927673945 1102 12-13 0.7391979097851689 1102 14-15 0.7224620864449989 1102 16-17 0.9769564863755207 1102 18-19 0.8888088254295354 1102 20-21 0.8098000253195536 1102 22-23 0.8683198969474262 1102 24-25 0.7380747958439358 1102 26-27 0.6991821420399091 1102 28-29 0.5563463310175152 1102 30-31 0.8362839149473587 1102 32-33 0.7967419802353888 1102 34-35 0.6126290780657726 1102 36-37 0.9329110427911971 1102 38-39 0.788224034020665 1102 40-41 0.9550422757384709 1102 42-43 1.0861079154658668 1102 44-45 1.0848021256688938 1102 46-47 1.017262428550623 1102 48-49 1.0381044049240984 1102 50-51 0.9952223297102236 1102 52-53 0.9273836951750667 1102 54-55 0.8477642984601701 1102 56-57 0.9676739407580897 1102 58-59 0.9385778632254187 1102 60-61 0.9338358422359896 1102 62-63 1.049652245194249 1102 64-65 1.2415300767789894 1102 66-67 0.9607457082952493 1102 68-69 1.0783597767348247 1102 70-71 1.016753251387989 1102 72-73 1.0483893843671659 1102 74-75 0.9524939939615678 1102 76-77 1.1202136837109755 1102 78-79 1.2698793034344256 1102 80-81 1.3999993526992434 1102 82-83 1.397517700741055 1102 84-85 1.4771145901365124 1102 86-87 1.691310383203085 1102 88-89 1.668520210208559 1102 90-91 1.6255276086425532 1102 92-93 1.882238692641117 1102 94-95 1.9154251550668455 1102 96-97 1.8772112131767251 1102 98-99 1.8936058983009776 1102 100-101 1.4275986814252235 1103 1 0.5363738595437297 1103 2 0.5102601904477062 1103 3 0.4335961981691234 1103 4 0.5240224323343483 1103 5 0.4920621344194842 1103 6 0.5299641391713479 1103 7 0.4602389109878047 1103 8 0.49495427764166067 1103 9 0.6313447200467621 1103 10-11 0.7613456937341283 1103 12-13 0.8414915523215925 1103 14-15 0.9453945638985246 1103 16-17 0.9193009089136055 1103 18-19 0.9193784170556114 1103 20-21 0.7561212446930412 1103 22-23 0.8457742636940395 1103 24-25 0.8796653801449281 1103 26-27 0.9199298678221197 1103 28-29 1.006208715698527 1103 30-31 1.061698598724817 1103 32-33 1.1322103760188824 1103 34-35 1.1211256370872107 1103 36-37 1.134024464224204 1103 38-39 1.211467800831194 1103 40-41 1.2032107940093013 1103 42-43 1.1543696316566852 1103 44-45 1.218347608152932 1103 46-47 0.951103910746049 1103 48-49 1.101916978883608 1103 50-51 1.2668451388324868 1103 52-53 1.183371076154927 1103 54-55 1.1038525043151282 1103 56-57 1.1823198046579506 1103 58-59 1.2163694530184301 1103 60-61 1.4008530050971721 1103 62-63 1.3464252450924903 1103 64-65 1.5927979006599706 1103 66-67 1.6048765264010498 1103 68-69 1.6617104672618446 1103 70-71 1.7481745026510644 1103 72-73 1.8053297518563909 1103 74-75 1.9799844279356513 1103 76-77 1.9475008002829064 1103 78-79 2.0161255550463437 1103 80-81 1.986846581827475 1103 82-83 1.9198357335379832 1103 84-85 2.0005609256075054 1103 86-87 2.1793276233207983 1103 88-89 2.0114506252190587 1103 90-91 2.0768140064224667 1103 92-93 2.037082642961426 1103 94-95 2.0963393125129315 1103 96-97 2.052780673773004 1103 98-99 2.0032865582691297 1103 100-101 1.68612489231921 1104 1 0.3677598519375067 1104 2 0.24395863803023943 1104 3 0.3633506715005872 1104 4 0.2287513827380394 1104 5 0.30546420430943755 1104 6 0.32319919949118514 1104 7 0.42316052637481505 1104 8 0.5548367163087065 1104 9 0.6154308823336123 1104 10-11 0.6450760936842528 1104 12-13 0.5715724658475736 1104 14-15 0.7381571578073078 1104 16-17 0.7894091767835434 1104 18-19 0.7194515762875042 1104 20-21 0.8508332252722397 1104 22-23 0.8372990930216346 1104 24-25 0.8374853324501785 1104 26-27 0.833482152806063 1104 28-29 0.7748273018452281 1104 30-31 0.8566247965489353 1104 32-33 0.8132789878833435 1104 34-35 0.7487904450612675 1104 36-37 0.9419225439648429 1104 38-39 0.9800162476343957 1104 40-41 0.9980210670851406 1104 42-43 1.1299742912470734 1104 44-45 1.0132703964256322 1104 46-47 0.8764045357655377 1104 48-49 0.7877969548803705 1104 50-51 1.0359108632939709 1104 52-53 0.9166340753132474 1104 54-55 0.8964801774034399 1104 56-57 0.8887929841523245 1104 58-59 0.9716601026646288 1104 60-61 0.9576317778145302 1104 62-63 1.0374663077814752 1104 64-65 1.1249923464032925 1104 66-67 1.162369843680267 1104 68-69 1.2013153294770014 1104 70-71 1.2900998081468735 1104 72-73 1.3785021348795539 1104 74-75 1.3029059653900639 1104 76-77 1.2094882959966178 1104 78-79 1.4334083763624719 1104 80-81 1.4622885571072537 1104 82-83 1.442005123949155 1104 84-85 1.5714644957000878 1104 86-87 1.6501545272143119 1104 88-89 1.7668908080489523 1104 90-91 1.6507121368192976 1104 92-93 1.6001345032126792 1104 94-95 1.9273648666137433 1104 96-97 2.0075286202488876 1104 98-99 2.1086451066964482 1104 100-101 1.8839407336796032 1105 1 0.3233484683162011 1105 2 0.2585608237116048 1105 3 0.31967755397727515 1105 4 0.3212692415375571 1105 5 0.28984856779900525 1105 6 0.31706129495709234 1105 7 0.28043226722225967 1105 8 0.2881758949669049 1105 9 0.41142058202495946 1105 10-11 0.41750349255542574 1105 12-13 0.4282870594768369 1105 14-15 0.5449949498854991 1105 16-17 0.5555333017086426 1105 18-19 0.6270009857373111 1105 20-21 0.6127476193142058 1105 22-23 0.5968412332693163 1105 24-25 0.548368277390658 1105 26-27 0.5045643904152328 1105 28-29 0.549414924361983 1105 30-31 0.6423240603827693 1105 32-33 0.6208919515288684 1105 34-35 0.5683185079488169 1105 36-37 0.6135721541929371 1105 38-39 0.7230942781767666 1105 40-41 0.7563904596477542 1105 42-43 0.9185555118010527 1105 44-45 1.0502436825074213 1105 46-47 0.7930120439834738 1105 48-49 0.621433132457895 1105 50-51 0.7186385786589327 1105 52-53 0.5854650009368925 1105 54-55 0.761037065734854 1105 56-57 0.709441296498909 1105 58-59 0.7706186162157493 1105 60-61 0.7879740443086867 1105 62-63 0.8661452424216556 1105 64-65 0.9890407071422658 1105 66-67 1.0391718256998566 1105 68-69 1.02645427739375 1105 70-71 1.0485645986052958 1105 72-73 0.9849665392017712 1105 74-75 1.1007207228137794 1105 76-77 1.214572514120949 1105 78-79 1.2969496199091068 1105 80-81 1.408810377250525 1105 82-83 1.429923832561748 1105 84-85 1.557824006999585 1105 86-87 1.7792909956476741 1105 88-89 1.7046899304039798 1105 90-91 1.6254958676347258 1105 92-93 1.5786499959876714 1105 94-95 1.6548189157784101 1105 96-97 1.7964475532718076 1105 98-99 1.9259542968008336 1105 100-101 1.4756916874234935 1106 1 0.291124514798188 1106 2 0.19638146110477805 1106 3 0.18523324665747154 1106 4 0.2196007820115824 1106 5 0.274239454832383 1106 6 0.037539325451177774 1106 7 0.29373200942534083 1106 8 0.1249984801261661 1106 9 0.06812414558848445 1106 10-11 9.116547272753905E-4 1106 12-13 0.061287125498850514 1106 14-15 0.4096905677889566 1106 16-17 0.13112792560361441 1106 18-19 0.015148533198313885 1106 20-21 0.2772507235542605 1106 22-23 0.2433529884929584 1106 24-25 0.11717215988951324 1106 26-27 0.06820747431365959 1106 28-29 0.32914837427977517 1106 30-31 0.027100752387418936 1106 32-33 0.07401635979003629 1106 34-35 -0.22514806543161825 1106 36-37 -0.02719677443150914 1106 38-39 0.05990123268231429 1106 40-41 0.3006207382147821 1106 42-43 -0.19405304444989468 1106 44-45 0.18388499415411985 1106 46-47 0.17061064988468644 1106 48-49 0.3668412391059235 1106 50-51 0.19734441807675296 1106 52-53 0.1866676514291754 1106 54-55 0.3421253589429014 1106 56-57 -0.5620318583976882 1106 58-59 -0.18273127005763712 1106 60-61 0.4062025982158204 1106 62-63 0.33194474997052126 1106 64-65 0.4694556146799478 1106 66-67 0.11963026577461378 1106 68-69 0.2154019354668968 1106 70-71 0.2525700974848277 1106 72-73 0.5891940322463256 1106 74-75 0.9537813797818231 1106 76-77 0.8945974018543588 1106 78-79 0.9581632103037059 1106 80-81 1.1081293240057555 1106 82-83 0.8103188090718305 1106 84-85 0.8363683218858711 1106 86-87 0.5906451802730679 1106 88-89 0.7570047563434308 1106 90-91 0.8418320470690261 1106 92-93 0.6898091396051527 1106 94-95 0.5919805772531319 1106 96-97 0.5243838162873402 1106 98-99 0.7310800547417298 1106 100-101 0.2476569357366465 1107 1 0.19404287856071534 1107 2 0.07331327281443478 1107 3 0.1773598848227671 1107 4 0.04821064990274948 1107 5 0.14485842777516922 1107 6 0.3070638672539303 1107 7 0.2795181513423657 1107 8 0.0972332016414228 1107 9 0.3252028207608362 1107 10-11 0.3943147266608804 1107 12-13 0.4422540966512756 1107 14-15 0.5477093393912398 1107 16-17 0.6160943698474739 1107 18-19 0.5704010616586785 1107 20-21 0.6077016031218037 1107 22-23 0.559218050399501 1107 24-25 0.5478230380853759 1107 26-27 0.47679372077745086 1107 28-29 0.6084705602316873 1107 30-31 0.810967412911296 1107 32-33 0.8799920456583052 1107 34-35 0.7381350624908691 1107 36-37 0.8817601270121358 1107 38-39 0.7300413360899825 1107 40-41 0.6829031543485016 1107 42-43 0.8671477807718162 1107 44-45 0.8059638870079908 1107 46-47 0.7215751132266703 1107 48-49 0.6618562129110757 1107 50-51 0.6427091136521241 1107 52-53 0.6613594904295397 1107 54-55 0.897532240004999 1107 56-57 0.8117601582367655 1107 58-59 0.7666561726406798 1107 60-61 0.7395920665450966 1107 62-63 0.8792675206806067 1107 64-65 1.0072453137516426 1107 66-67 0.9817141846861617 1107 68-69 1.1716504090870288 1107 70-71 1.2169300034702637 1107 72-73 1.3157871553589437 1107 74-75 1.3319653075497264 1107 76-77 1.337601818403762 1107 78-79 1.3413555277561287 1107 80-81 1.3950375247248168 1107 82-83 1.2786010081204147 1107 84-85 1.296140328817092 1107 86-87 1.4313973161612097 1107 88-89 1.3481399968054752 1107 90-91 1.302744421905878 1107 92-93 1.5047633683371444 1107 94-95 1.6764734001739825 1107 96-97 1.5706699532841633 1107 98-99 1.6634907240587413 1107 100-101 1.3721724452470916 1108 1 0.43535323680709226 1108 2 0.2444921956052788 1108 3 0.3089068991255921 1108 4 0.2757855894338235 1108 5 0.3478144147819222 1108 6 0.33263849686716185 1108 7 0.3642675722853568 1108 8 0.4754780180908327 1108 9 0.43759638845108384 1108 10-11 0.5248166420929223 1108 12-13 0.43221497243713713 1108 14-15 0.6123113338173809 1108 16-17 0.5958722309949636 1108 18-19 0.6550970334258679 1108 20-21 0.5167124642273535 1108 22-23 0.45442358651075665 1108 24-25 0.733262113598137 1108 26-27 0.7500848909153532 1108 28-29 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2316 90-91 -3.8722219593515277 2316 92-93 -3.783196270743094 2316 94-95 -4.0919896040326265 2316 96-97 -4.127660776550146 2316 98-99 -3.935406713978976 2316 100-101 -3.1666748180707263 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 15568.0 3 5880.0 4 6108.0 5 7136.0 6 8272.0 7 10285.0 8 12923.0 9 15911.0 10 19854.0 11 23741.0 12 28176.0 13 32625.0 14 35737.0 15 41150.0 16 46442.0 17 51409.0 18 50764.0 19 50458.0 20 53811.0 21 57998.0 22 63731.0 23 70120.0 24 77285.0 25 85725.0 26 95292.0 27 106664.0 28 119584.0 29 135081.0 30 153503.0 31 175398.0 32 203673.0 33 239187.0 34 282748.0 35 332485.0 36 367951.0 37 326094.0 38 129592.0 39 5523.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24304189256459 18.166312792848927 14.576327816965398 24.01431749762109 2 15.543372731974648 19.29723663392878 40.166360699396265 24.993029934700303 3 19.51518205956659 22.9917032106024 29.132270920161375 28.360843809669632 4 13.12563811796799 15.939967895638791 36.05064940421606 34.883744582177165 5 14.27478907499923 36.73778941159746 33.92754744240454 15.059874070998768 6 33.695529372421994 35.12935344179915 16.831820754621056 14.343296431157803 7 29.564693875016513 31.793469363792475 19.99442834353016 18.647408417660852 8 26.79117235882184 34.837903343487866 19.216114856769153 19.154809440921138 9 26.31457138220331 16.731257452918722 19.54626983622983 37.40790132864814 10-11 25.39632775196276 26.05181812612416 27.026809945904684 21.525044176008397 12-13 26.329399933483423 23.18227067731158 27.218706292028116 23.269623097176883 14-15 22.808150276976644 24.84148569899557 25.224180882430147 27.126183141597643 16-17 22.822195055653047 27.887507257773827 26.270685537490195 23.01961214908293 18-19 22.937529313148904 26.490350001997143 28.20159570796927 22.37052497688469 20-21 23.112480456825025 25.808764518417348 28.56928077586846 22.509474248889173 22-23 22.90035547259423 25.702473658516826 28.48675258771544 22.9104182811735 24-25 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27.142492700160375 23.269958468448916 50-51 23.142371798951032 26.845260780432923 27.11764034412048 22.894727076495567 52-53 23.421513962662008 26.441376686810468 27.229857751694503 22.907251598833025 54-55 22.889449356295 26.72640752527677 27.402086823907972 22.98205629452026 56-57 22.913152175002036 26.87989056728429 27.16819000698348 23.038767250730203 58-59 22.88683803853108 26.3832479841973 27.22630148553924 23.503612491732383 60-61 22.522567274928786 26.676484098653198 27.500781466645307 23.30016715977271 62-63 22.727168181913747 26.636698963999105 27.673588813713295 22.962544040373853 64-65 22.47723043527585 26.718575122208094 27.464057190170525 23.34013725234553 66-67 22.3973539331685 26.710504389204697 27.438057063823624 23.454084613803182 68-69 23.008707828861926 26.359427093945552 27.519502025975612 23.11236305121691 70-71 22.784729021004928 26.471207965763917 27.101371326134625 23.642691687096534 72-73 22.69605668118583 26.54261871052673 27.190660542876692 23.57066406541075 74-75 22.728737904525563 26.857330276663955 27.10681840120987 23.30711341760061 76-77 22.904447132939826 26.437036923095597 27.167728760325936 23.490787183638645 78-79 22.88664663775441 26.732178037681127 27.093677657923802 23.287497666640657 80-81 23.12446445671625 26.680634119932627 26.81594411739433 23.378957305956785 82-83 22.92013615760419 26.720682904460396 26.96071535653186 23.398465581403553 84-85 22.83286597825268 26.820462561598735 26.94286284311233 23.403808617036255 86-87 23.1910453237004 26.77198453462776 26.570215047694308 23.466755093977536 88-89 22.972645298387725 26.802009952091304 26.465107836904938 23.76023691261603 90-91 22.805568281480717 26.818948709675873 26.5337797804291 23.841703228414314 92-93 23.003467860894915 26.370660304875948 26.814925293932685 23.810946540296456 94-95 22.787327843678508 26.150526002131137 26.966888310880254 24.0952578433101 96-97 23.086870765025857 25.833289342156412 26.887242662201118 24.192597230616617 98-99 22.820225542062033 26.09161423780661 26.523200626140376 24.564959593990984 100-101 23.406511685959835 25.348987562263865 26.862197688716748 24.382303063059553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 340.0 1 255.5 2 259.0 3 358.0 4 323.0 5 310.5 6 543.5 7 1155.5 8 1847.5 9 2127.0 10 1915.0 11 1664.5 12 1656.5 13 1766.0 14 1990.0 15 2228.5 16 2451.5 17 2808.0 18 3206.5 19 3681.0 20 4329.0 21 5244.5 22 6497.0 23 8126.0 24 10326.0 25 13214.5 26 16975.0 27 21633.0 28 26164.0 29 30732.5 30 36020.5 31 44051.0 32 55179.5 33 59865.0 34 60003.5 35 64082.0 36 70294.0 37 77625.0 38 84835.0 39 90170.5 40 95203.5 41 102222.0 42 107444.5 43 111698.0 44 117079.0 45 124393.0 46 129055.0 47 131833.5 48 133485.5 49 132482.5 50 132575.0 51 131301.5 52 130110.0 53 130632.5 54 131650.0 55 125896.0 56 116146.0 57 107291.0 58 99970.5 59 88718.5 60 72171.5 61 60387.5 62 51423.0 63 40038.5 64 29237.0 65 22425.5 66 17237.0 67 13286.5 68 10385.0 69 8081.5 70 6391.5 71 4695.5 72 3402.5 73 2663.0 74 2068.5 75 1536.5 76 1082.0 77 714.5 78 482.0 79 314.0 80 199.5 81 122.5 82 84.0 83 59.0 84 32.5 85 16.0 86 13.0 87 14.0 88 10.5 89 7.0 90 7.0 91 5.5 92 5.0 93 3.5 94 1.5 95 1.5 96 2.0 97 1.5 98 0.5 99 0.5 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006997971716907212 2 0.10567501645087705 3 0.1151561394221707 4 0.11789324932757392 5 0.15635387614267285 6 0.09458548868981038 7 0.026863181751998658 8 0.02728644617035998 9 0.022828060963620705 10-11 0.05087638308703107 12-13 0.05389566927134184 14-15 0.02782258110028432 16-17 0.03191413714444378 18-19 0.03720494237396032 20-21 0.057747375478429876 22-23 0.032041116469952176 24-25 0.04915510778569502 26-27 0.0566751056185812 28-29 0.05333131671352674 30-31 0.06744013065890418 32-33 0.05028381290132521 34-35 0.06207878135966075 36-37 0.052696420085984755 38-39 0.031011173051939622 40-41 0.03662648100219985 42-43 0.03205522528389756 44-45 0.007280147995814762 46-47 0.013022435271583383 48-49 0.008832117529806281 50-51 0.008451179553281089 52-53 0.010214781296453269 54-55 0.011653880318881768 56-57 0.014659057689247164 58-59 0.01954070731434776 60-61 0.02445057456733911 62-63 0.011357595226028843 64-65 0.009537558227075152 66-67 0.010172454854617138 68-69 0.011456356923646487 70-71 0.030263405912834617 72-73 0.027653275332939792 74-75 0.029064156727477534 76-77 0.022672864010221554 78-79 0.004895758439045973 80-81 0.004782887927482954 82-83 0.005544763880533336 84-85 0.005163825904008145 86-87 0.013657331899125366 88-89 0.037021527792670414 90-91 0.03518738197977135 92-93 0.030079991331544712 94-95 0.04643210669423717 96-97 0.042523965231367616 98-99 0.02563571493875082 100-101 0.0734928118414711 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3543884.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.9096496021116 #Duplication Level Percentage of deduplicated Percentage of total 1 75.45382085658034 40.67689043518746 2 13.478805158910353 14.532753263439826 3 4.735275829459261 7.658311822064913 4 2.1683319921496675 4.675760716713486 5 1.1903073587259203 3.208452631386466 6 0.698500932540437 2.259356431200189 7 0.45395740669648166 1.7130879310503415 8 0.3198340525238527 1.3793713361907405 9 0.23907737195006637 1.1599719614659603 >10 1.1379703734041828 10.860595338540262 >50 0.07713948442931597 2.8344695865283724 >100 0.0409273604683391 4.167205015431236 >500 0.002789100658656299 0.9805988697544032 >1k 0.003104847882014504 3.3474833792186054 >5k 1.5787362111938157E-4 0.5456912818277883 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7937 0.22396331256892155 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6293 0.1775735323165205 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 5005 0.1412292275932282 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4977 0.14043913401228708 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4445 0.1254273559744055 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4369 0.1232828162547081 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4265 0.12034818295406961 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3910 0.11033092505285161 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3823 0.10787599142635595 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.821762789075489E-5 2 0.0 0.0 0.0 0.0 5.643525578150978E-5 3 0.0 0.0 0.0 0.0 5.643525578150978E-5 4 0.0 0.0 0.0 2.821762789075489E-5 5.643525578150978E-5 5 0.0 0.0 0.0 2.821762789075489E-5 5.643525578150978E-5 6 0.0 0.0 0.0 2.821762789075489E-5 8.465288367226466E-5 7 0.0 0.0 0.0 2.821762789075489E-5 8.465288367226466E-5 8 0.0 0.0 0.0 2.821762789075489E-5 8.465288367226466E-5 9 0.0 0.0 0.0 2.821762789075489E-5 8.465288367226466E-5 10-11 0.0 0.0 0.0 2.821762789075489E-5 8.465288367226466E-5 12-13 0.0 0.0 0.0 5.643525578150978E-5 3.245027207436812E-4 14-15 1.4108813945377445E-5 0.0 0.0 5.643525578150978E-5 3.6682916257981354E-4 16-17 4.232644183613234E-5 0.0 0.0 5.643525578150978E-5 3.950467904705685E-4 18-19 5.643525578150978E-5 0.0 0.0 9.876169761764212E-5 4.5148204625207824E-4 20-21 5.643525578150978E-5 0.0 0.0 1.26979325508397E-4 5.361349299243429E-4 22-23 5.643525578150978E-5 0.0 0.0 2.6806746496217145E-4 5.643525578150978E-4 24-25 7.054406972688722E-5 0.0 0.0 5.502437438697203E-4 5.643525578150978E-4 26-27 9.876169761764212E-5 1.4108813945377445E-5 0.0 8.183112088318918E-4 5.784613717604752E-4 28-29 1.1287051156301956E-4 2.821762789075489E-5 0.0 0.00248315125438643 5.925701857058527E-4 30-31 1.1287051156301956E-4 2.821762789075489E-5 0.0 0.008437070739335712 5.925701857058527E-4 32-33 1.4108813945377444E-4 2.821762789075489E-5 0.0 0.0220802938245157 6.207878135966075E-4 34-35 1.4108813945377444E-4 2.821762789075489E-5 0.0 0.04195961267355252 6.772230693781173E-4 36-37 1.4108813945377444E-4 2.821762789075489E-5 0.0 0.07614526886320207 6.772230693781173E-4 38-39 1.5519695339915189E-4 2.821762789075489E-5 0.0 0.12858773029817003 7.054406972688722E-4 40-41 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.18468437454499076 7.054406972688722E-4 42-43 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.24388495785979453 7.054406972688722E-4 44-45 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.32029829418795874 7.054406972688722E-4 46-47 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.4119068231352945 7.054406972688722E-4 48-49 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.5066192911506133 7.054406972688722E-4 50-51 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.6119839136946921 8.465288367226467E-4 52-53 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.717955215238422 8.747464646134016E-4 54-55 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.8357497028683782 9.029640925041565E-4 56-57 2.2574102312603912E-4 2.821762789075489E-5 0.0 0.9747497378582368 9.170729064495339E-4 58-59 2.2574102312603912E-4 2.821762789075489E-5 0.0 1.1232591134472798 9.311817203949114E-4 60-61 2.2574102312603912E-4 4.232644183613234E-5 0.0 1.2855104738191203 9.311817203949114E-4 62-63 2.2574102312603912E-4 5.643525578150978E-5 0.0 1.4478041606327974 9.311817203949114E-4 64-65 2.2574102312603912E-4 5.643525578150978E-5 0.0 1.6201715406034736 9.311817203949114E-4 66-67 2.2574102312603912E-4 5.643525578150978E-5 0.0 1.82095407186014 9.452905343402888E-4 68-69 2.821762789075489E-4 5.643525578150978E-5 0.0 2.0462295041259813 9.593993482856663E-4 70-71 2.821762789075489E-4 5.643525578150978E-5 0.0 2.2840758896171547 9.876169761764211E-4 72-73 2.821762789075489E-4 5.643525578150978E-5 0.0 2.5343662490081504 0.0010863786737940633 74-75 2.821762789075489E-4 5.643525578150978E-5 0.0 2.784698934840982 0.0011851403714117053 76-77 2.821762789075489E-4 7.054406972688722E-5 0.0 3.059990112543187 0.0012133579993024602 78-79 2.821762789075489E-4 8.465288367226466E-5 0.0 3.3727401912703687 0.0012274668132478375 80-81 2.821762789075489E-4 8.465288367226466E-5 0.0 3.6968337564096343 0.001241575627193215 82-83 2.821762789075489E-4 8.465288367226466E-5 0.0 4.034937373796659 0.0012980108829747249 84-85 2.821762789075489E-4 8.465288367226466E-5 0.0 4.387446090221915 0.0013121196969201024 86-87 2.821762789075489E-4 8.465288367226466E-5 0.0 4.747658219061346 0.0013826637666469895 88-89 2.821762789075489E-4 8.465288367226466E-5 0.0 5.133506062839529 0.0014390990224284993 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4835 0.0 53.11513 1 GTATCAA 9110 0.0 42.807167 1 TATCAAC 11690 0.0 33.114532 2 ATCAACG 11690 0.0 32.99249 3 TCAACGC 11810 0.0 32.657257 4 CAACGCA 11980 0.0 31.995358 5 AACGCAG 12585 0.0 30.472198 6 ACGCAGA 14185 0.0 26.838247 7 CGCAGAG 14285 0.0 26.649616 8 TGGTATC 2470 0.0 23.874456 2 GTACATG 10880 0.0 23.735147 1 TACATGG 10510 0.0 23.348326 2 GTAAGGT 2060 0.0 23.316477 4 TAAGGTG 2170 0.0 22.791996 5 GCAGAGT 16105 0.0 22.781536 9 GTGGTAT 2855 0.0 22.32131 1 ACATGGG 10630 0.0 22.01104 3 GAGTACT 9935 0.0 20.926653 12-13 AGAGTAC 14220 0.0 20.720934 10-11 CAGAGTA 15185 0.0 19.810987 10-11 >>END_MODULE