FastQCFastQC Report
Thu 26 May 2016
SRR938531_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938531_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3543884
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81130.2289296150776944No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67850.19145660523877192No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA48450.13671440713070745No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47710.13462630266679157No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47290.1334411622953799No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46970.1325381982028757No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45450.12824911876348097No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42290.11933234835000243No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38680.10914578468143991No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35590.10042653766319666No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52000.052.8364371
GTATCAA88650.042.1456571
ATCAACG117000.031.5089133
TCAACGC118000.031.2016284
TATCAAC119000.030.9972922
CAACGCA121500.030.3028165
AACGCAG127000.029.0862876
ACGCAGA143250.025.621077
CGCAGAG144250.025.3447078
TAAGGTG23200.025.18695
GTAAGGT22050.024.3459914
TGGTATC26250.021.9111442
GCAGAGT166750.021.611679
AGAGTAC145350.021.41263210-11
GTACATG106500.021.379371
TACATGG104100.021.2329942
GAGTACT103350.020.85728512-13
GTGGTAT28950.020.8527891
ACATGGG103400.020.3076323
AGGTAAG23600.019.9403232
Produced by FastQC (version 0.11.5)