FastQCFastQC Report
Thu 26 May 2016
SRR938530_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938530_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503220
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82180.23458418255205213No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62180.17749384851650768No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA50570.14435290960887412No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48710.1390435085435685No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43640.12457110886555797No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43200.123315121516776No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42750.12203058900097624No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC37640.10744400865489465No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA37190.10615947613909489No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43900.053.137911
GTATCAA83400.046.0659871
ATCAACG110950.034.3981063
TCAACGC111500.034.1858064
TATCAAC112850.033.9448282
CAACGCA113750.033.3823475
AACGCAG118850.032.062966
ACGCAGA135900.027.9310677
CGCAGAG138150.027.4077788
TAAGGTG23900.025.0549835
GTGGTAT24200.024.3937851
TGGTATC22300.023.8700472
GCAGAGT155750.023.6077449
GTACATG103450.023.3319151
GTAAGGT23100.023.2494544
TACATGG101400.023.1072942
ACATGGG101750.022.047223
AGAGTAC137050.021.47309110-11
AGGTAAG24050.020.9474372
GGTAAGG26150.020.9012583