FastQCFastQC Report
Thu 26 May 2016
SRR938530_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938530_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3503220
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78700.22465046442986739No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67790.19350768721347789No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA49460.1411843960699014No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA48810.13932896021374622No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.13353429130913844No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46460.13262084596456974No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45740.13056559393929013No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA41870.11951861430341229No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38450.1097561671833342No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36140.10316223360222881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50850.054.8086931
GTATCAA87900.041.0131531
TATCAAC115950.030.9269582
ATCAACG115850.030.8967043
TCAACGC118250.030.1892264
CAACGCA120950.029.5546055
AACGCAG126200.028.452216
ACGCAGA144750.024.6419417
CGCAGAG146250.024.3892028
TAAGGTG23200.024.1772675
TGGTATC24000.023.7797152
GGACCGA9800.023.2631596
AGGTAAG22200.022.4943242
GTAAGGT23600.022.357554
GTGGTAT28850.021.595751
AGGACCG13050.021.4909045
TACATGG98950.020.8598182
TAGTCCG1604.964808E-520.796555
GCAGAGT168500.020.6894879
GTACATG102150.020.5325571
Produced by FastQC (version 0.11.5)