##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938530_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3503220 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.788267080000686 31.0 31.0 34.0 30.0 34.0 2 32.01219049902661 31.0 31.0 34.0 30.0 34.0 3 32.08387454969999 33.0 31.0 34.0 30.0 34.0 4 35.753803928956785 37.0 35.0 37.0 35.0 37.0 5 35.61467535581551 37.0 35.0 37.0 33.0 37.0 6 35.64224913079966 37.0 35.0 37.0 33.0 37.0 7 35.5810679888788 37.0 35.0 37.0 33.0 37.0 8 35.562763971432 37.0 35.0 37.0 33.0 37.0 9 37.151792921940384 39.0 37.0 39.0 33.0 39.0 10-11 37.05806343877918 39.0 37.0 39.0 33.0 39.0 12-13 36.950025405198645 39.0 37.0 39.0 33.0 39.0 14-15 38.00711545378252 40.0 37.0 41.0 33.0 41.0 16-17 37.92420872797027 40.0 37.0 41.0 33.0 41.0 18-19 38.024534285600105 40.0 37.0 41.0 33.0 41.0 20-21 38.06835411421493 40.0 37.0 41.0 33.0 41.0 22-23 37.97714188660718 40.0 37.0 41.0 33.0 41.0 24-25 37.87687142114969 40.0 37.0 41.0 32.0 41.0 26-27 37.678134972967726 40.0 37.0 41.0 32.0 41.0 28-29 37.5588213129635 39.5 37.0 41.0 32.0 41.0 30-31 37.3227583480341 39.0 36.0 41.0 31.0 41.0 32-33 37.11493996951376 39.0 36.0 41.0 31.0 41.0 34-35 36.96794449106822 39.0 36.0 41.0 30.5 41.0 36-37 36.97230191081348 39.0 36.0 41.0 30.5 41.0 38-39 36.84395427635147 39.0 35.5 41.0 30.0 41.0 40-41 36.67015074702702 39.0 35.0 41.0 30.0 41.0 42-43 36.45861450322846 39.0 35.0 40.0 30.0 41.0 44-45 36.20605814079618 39.0 35.0 40.0 30.0 41.0 46-47 35.86302815695275 38.0 35.0 40.0 28.5 41.0 48-49 35.50908478485508 38.0 34.5 40.0 27.5 41.0 50-51 35.13759084499404 38.0 34.0 40.0 26.5 41.0 52-53 34.63492101552286 38.0 33.0 40.0 26.0 41.0 54-55 34.31069958495327 37.5 33.0 40.0 24.5 41.0 56-57 33.964575019553436 37.0 33.0 40.0 24.0 41.0 58-59 33.51551315646748 36.5 32.0 39.0 23.0 41.0 60-61 33.136942869702736 36.0 32.0 39.0 22.0 40.0 62-63 32.66153838468609 35.5 31.0 39.0 21.5 40.0 64-65 32.148737019085296 35.0 31.0 38.5 19.5 40.0 66-67 31.651815615348166 35.0 30.0 38.0 18.0 40.0 68-69 31.101212027791576 34.5 29.5 37.5 15.5 39.5 70-71 30.514052642997015 34.0 29.0 37.0 12.0 39.0 72-73 30.06583015054721 34.0 29.0 36.0 8.0 39.0 74-75 29.54589648951536 34.0 28.0 36.0 7.5 38.5 76-77 28.302575202242508 31.5 26.5 34.5 7.0 36.5 78-79 29.001307939552753 33.0 28.0 35.0 4.5 37.0 80-81 28.734437745845252 33.0 28.0 35.0 2.0 37.0 82-83 28.261068816688645 33.0 27.0 35.0 2.0 36.0 84-85 27.7301462654358 33.0 26.0 35.0 2.0 36.0 86-87 27.15669227168148 32.5 25.5 35.0 2.0 35.0 88-89 26.50323316834227 32.0 25.0 34.0 2.0 35.0 90-91 25.957867333481765 32.0 23.5 34.0 2.0 35.0 92-93 25.220590057147426 31.0 20.5 34.0 2.0 35.0 94-95 24.44084770582493 31.0 17.5 34.0 2.0 35.0 96-97 23.42921626389436 31.0 4.5 34.0 2.0 35.0 98-99 22.177664263163607 30.0 2.0 34.0 2.0 35.0 100-101 18.518052106347874 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.266919756798643 1101 2 0.3019344409556126 1101 3 0.31829646128555567 1101 4 0.20524537975996537 1101 5 0.17353714336907444 1101 6 0.18091781624234926 1101 7 0.3441979539520972 1101 8 0.2232908219919736 1101 9 0.2651111978783973 1101 10-11 0.3043624663364568 1101 12-13 0.43030133627105016 1101 14-15 0.4220074112362724 1101 16-17 0.3833951997973344 1101 18-19 0.461480630721816 1101 20-21 0.5269326911871985 1101 22-23 0.41332326202101655 1101 24-25 0.44398838651525097 1101 26-27 0.5155394201896399 1101 28-29 0.5335087656501116 1101 30-31 0.45063257185629624 1101 32-33 0.35930485286848324 1101 34-35 0.2962730412947039 1101 36-37 0.4779265348811137 1101 38-39 0.6219745941932331 1101 40-41 0.5407242526190217 1101 42-43 0.6003381231424569 1101 44-45 0.6316502857138957 1101 46-47 0.607430989540525 1101 48-49 0.7954091308446465 1101 50-51 0.8911675014397105 1101 52-53 1.0003150860425833 1101 54-55 0.8979063229826636 1101 56-57 0.841413722156723 1101 58-59 0.8263064588786833 1101 60-61 0.8274286511960085 1101 62-63 0.8623265882649278 1101 64-65 0.9820122473673578 1101 66-67 0.8508803544697514 1101 68-69 0.7371651745618379 1101 70-71 0.7392707673410897 1101 72-73 0.7156880664377852 1101 74-75 0.8279314073896771 1101 76-77 0.8104571932651758 1101 78-79 0.6960596103021395 1101 80-81 0.851166358641283 1101 82-83 0.8385391090260761 1101 84-85 0.8451033497801497 1101 86-87 0.9631763812819081 1101 88-89 0.8196967563473336 1101 90-91 0.9957588023176243 1101 92-93 0.8784913349521588 1101 94-95 0.9689798698178862 1101 96-97 0.9455354469077797 1101 98-99 0.986171562011755 1101 100-101 0.8733629690390963 1102 1 0.31683357708394055 1102 2 0.3769473715707008 1102 3 0.3713333817580633 1102 4 0.2704886545598626 1102 5 0.18680428365838253 1102 6 0.21423234805897806 1102 7 0.35103618262974834 1102 8 0.30274259317531715 1102 9 0.3787531979793499 1102 10-11 0.39393349546709544 1102 12-13 0.4733036787790965 1102 14-15 0.5646256029107874 1102 16-17 0.5536950583835889 1102 18-19 0.5432897243643495 1102 20-21 0.5104356405379633 1102 22-23 0.5367365639843484 1102 24-25 0.5893881235866445 1102 26-27 0.5138892578893888 1102 28-29 0.5346396915114155 1102 30-31 0.4835165708876872 1102 32-33 0.6232820796228253 1102 34-35 0.5981794016941748 1102 36-37 0.6531050029744989 1102 38-39 0.6483877074098245 1102 40-41 0.7069290201569274 1102 42-43 0.6527109550239771 1102 44-45 0.7084692044291643 1102 46-47 0.699107313017322 1102 48-49 0.6981556337259605 1102 50-51 0.734728366046113 1102 52-53 0.62538312265945 1102 54-55 0.705112406626732 1102 56-57 0.7824725218883799 1102 58-59 0.7007322627532702 1102 60-61 0.6908136850423929 1102 62-63 0.6528678158942398 1102 64-65 0.842389038541036 1102 66-67 0.8514965247983213 1102 68-69 0.733005704779238 1102 70-71 0.662353177241954 1102 72-73 0.5309763331939941 1102 74-75 0.5513076915808206 1102 76-77 0.6816283046250255 1102 78-79 0.646007339159393 1102 80-81 0.629497665507639 1102 82-83 0.525682145405181 1102 84-85 0.5326375967062482 1102 86-87 0.290789700656763 1102 88-89 0.584016269468524 1102 90-91 0.529294270244943 1102 92-93 0.5764523763074472 1102 94-95 0.5568210862540681 1102 96-97 0.5145980084059296 1102 98-99 0.4725740234741309 1102 100-101 0.3774633640172631 1103 1 0.254839388613199 1103 2 0.25249876428189566 1103 3 0.1953895358397375 1103 4 0.11363346462295709 1103 5 0.15359920047838926 1103 6 0.1291634199032714 1103 7 0.24945092913462474 1103 8 0.20536052478371403 1103 9 0.3029088370848996 1103 10-11 0.285851906844087 1103 12-13 0.3071534449620259 1103 14-15 0.4054466190297532 1103 16-17 0.3507483795074293 1103 18-19 0.3674915799526062 1103 20-21 0.4596078414291256 1103 22-23 0.43170771649293727 1103 24-25 0.40008260537729967 1103 26-27 0.5155769157143695 1103 28-29 0.43896919815046687 1103 30-31 0.46649152130785154 1103 32-33 0.47025258742203135 1103 34-35 0.42006234350343874 1103 36-37 0.2821114206994011 1103 38-39 0.35519402535073397 1103 40-41 0.4242429491330242 1103 42-43 0.4244596527233355 1103 44-45 0.42696364974607803 1103 46-47 0.37959380129094455 1103 48-49 0.4555594157747933 1103 50-51 0.5216110273557888 1103 52-53 0.5440793518130178 1103 54-55 0.5709464672951867 1103 56-57 0.3601669050205629 1103 58-59 0.37175210998915276 1103 60-61 0.35068617995691653 1103 62-63 0.24962355246239554 1103 64-65 0.4507428612403288 1103 66-67 0.39230094835586016 1103 68-69 0.20410778499579152 1103 70-71 0.09130751349289312 1103 72-73 0.05793942138359398 1103 74-75 0.13002545852980774 1103 76-77 0.07568893263105281 1103 78-79 0.19840413333935203 1103 80-81 0.11858143956531464 1103 82-83 0.09193597061929992 1103 84-85 0.08478114988501062 1103 86-87 0.19897458171174875 1103 88-89 0.3218607453765969 1103 90-91 0.3537448383928883 1103 92-93 0.06368173158223911 1103 94-95 0.07831847743210218 1103 96-97 -0.027356991019882315 1103 98-99 -0.24141682080850657 1103 100-101 -0.4392239605495938 1104 1 0.27534301522189963 1104 2 0.2710772810661908 1104 3 0.2667320468179142 1104 4 0.10771670965065283 1104 5 0.11522768183380805 1104 6 0.12506685716558508 1104 7 0.12415825810594328 1104 8 0.11203121073236133 1104 9 0.2688029435056265 1104 10-11 0.26820707131008703 1104 12-13 0.2723736904401832 1104 14-15 0.3905641015671577 1104 16-17 0.4442296961157055 1104 18-19 0.4588275877461925 1104 20-21 0.3681192339866399 1104 22-23 0.38852666464377705 1104 24-25 0.40660387332427916 1104 26-27 0.4968072441994025 1104 28-29 0.4882498857138131 1104 30-31 0.5335464854153713 1104 32-33 0.5554417465860269 1104 34-35 0.47648821747762327 1104 36-37 0.5282426698746718 1104 38-39 0.5339963731505009 1104 40-41 0.5377248423615484 1104 42-43 0.5582103874663318 1104 44-45 0.4749795685593128 1104 46-47 0.40178263469861264 1104 48-49 0.4236899728351631 1104 50-51 0.5844230132436081 1104 52-53 0.705046093192955 1104 54-55 0.6428763461784897 1104 56-57 0.4889325188690705 1104 58-59 0.570276235106526 1104 60-61 0.5387329189518866 1104 62-63 0.5238718627134347 1104 64-65 0.5856286251772858 1104 66-67 0.47298180721637095 1104 68-69 0.24484340401426152 1104 70-71 0.1011057501438124 1104 72-73 0.041098996687424005 1104 74-75 0.2496015709953241 1104 76-77 0.2455891082358015 1104 78-79 0.16565167666839997 1104 80-81 0.29010259193235655 1104 82-83 0.10275932889145878 1104 84-85 0.18298682879266082 1104 86-87 0.45360530719470304 1104 88-89 0.3848140714646462 1104 90-91 0.3273476581000274 1104 92-93 0.4106955082853645 1104 94-95 0.36475897930667145 1104 96-97 0.27645991089675803 1104 98-99 0.12644578696339437 1104 100-101 -0.11395610295739189 1105 1 0.2572645847377686 1105 2 0.27320560250338843 1105 3 0.20262297113595196 1105 4 0.1939562358220357 1105 5 0.10115060283500554 1105 6 0.1573790005828286 1105 7 0.20912814809435787 1105 8 0.19355028219275283 1105 9 0.20128957663850144 1105 10-11 0.2530861720802733 1105 12-13 0.35384355497253495 1105 14-15 0.46638832370184247 1105 16-17 0.34552266904969287 1105 18-19 0.38332247984782697 1105 20-21 0.3721144955056346 1105 22-23 0.32937226377831763 1105 24-25 0.3114086862490453 1105 26-27 0.2981084507678986 1105 28-29 0.29294478692603576 1105 30-31 0.4001536896506721 1105 32-33 0.2560896946449418 1105 34-35 0.27576280504683126 1105 36-37 0.2547810136425497 1105 38-39 0.27204170832153807 1105 40-41 0.16922170980465268 1105 42-43 0.2911628055849107 1105 44-45 0.273259177427569 1105 46-47 0.07777194364090434 1105 48-49 -0.050653242564749235 1105 50-51 -0.1506815989802135 1105 52-53 -0.08008868872413899 1105 54-55 -0.042124529558670076 1105 56-57 0.09187134174678135 1105 58-59 0.18590411185437716 1105 60-61 0.09653071891501241 1105 62-63 0.2724315247738929 1105 64-65 0.2850574837738691 1105 66-67 0.20622471672945508 1105 68-69 0.1280387021704854 1105 70-71 0.10623204021183241 1105 72-73 0.06983036169491541 1105 74-75 -0.17146065067798943 1105 76-77 -0.06069685758292209 1105 78-79 -0.07901978548136768 1105 80-81 -0.04017028971548342 1105 82-83 0.07937763391741015 1105 84-85 -0.0042001012626151635 1105 86-87 0.17067801627725387 1105 88-89 0.12934107961277874 1105 90-91 0.06237833813246496 1105 92-93 0.07201299211589784 1105 94-95 0.04888521407779933 1105 96-97 -0.03885674421115226 1105 98-99 -0.16675564257094067 1105 100-101 -0.3674044245992114 1106 1 0.0996356266167382 1106 2 0.08623018526262172 1106 3 0.1173046513807634 1106 4 0.07467363759993617 1106 5 -0.005869060114854108 1106 6 -0.015450882801651744 1106 7 0.0570691670345127 1106 8 0.06350281751232956 1106 9 -0.046301879797709944 1106 10-11 0.017020537168278338 1106 12-13 0.00829390676573638 1106 14-15 0.036624688730206856 1106 16-17 0.043646538308315996 1106 18-19 0.15699873788452834 1106 20-21 0.11137974543149198 1106 22-23 0.17145473129019706 1106 24-25 0.07693680015923832 1106 26-27 0.02518110911181992 1106 28-29 0.1885656482837348 1106 30-31 0.11800644614371691 1106 32-33 0.19592695522559467 1106 34-35 0.07779956961790901 1106 36-37 -0.35355857705317106 1106 38-39 -0.08188021600486195 1106 40-41 0.14200043271102203 1106 42-43 0.21323517633801714 1106 44-45 0.11888208064070938 1106 46-47 -0.16293228325164222 1106 48-49 -0.048759470821849504 1106 50-51 2.8803843267866114E-4 1106 52-53 0.0846947892268517 1106 54-55 -0.33144604263413413 1106 56-57 -0.00852893273523847 1106 58-59 0.11983879718714974 1106 60-61 -0.28169648475255116 1106 62-63 -0.13111315200668372 1106 64-65 -0.5452364356121038 1106 66-67 -0.23564405231655172 1106 68-69 -1.0713766504894195 1106 70-71 -0.8247139431516501 1106 72-73 -0.477150096056814 1106 74-75 -0.29829264547475987 1106 76-77 -0.19705762912573377 1106 78-79 -0.28227217399979665 1106 80-81 -1.0254507544937148 1106 82-83 -0.4072687495317133 1106 84-85 -0.15914097046670506 1106 86-87 -0.0939127325517859 1106 88-89 0.059802210829019486 1106 90-91 -0.14903738056642268 1106 92-93 -0.30323467629370526 1106 94-95 -0.08758827260635371 1106 96-97 -0.0068201897269339895 1106 98-99 -0.34820203788564186 1106 100-101 -0.7717440086854523 1107 1 0.14588319102523428 1107 2 0.18866408557999392 1107 3 0.17990882552949472 1107 4 0.1667163649151604 1107 5 0.13923986170337344 1107 6 0.18483787279398456 1107 7 0.14700304359929106 1107 8 0.1351409601578979 1107 9 0.18614455049069534 1107 10-11 0.22493794349087892 1107 12-13 0.22211902150979057 1107 14-15 0.3707494586625302 1107 16-17 0.2999301268585768 1107 18-19 0.2625506133707489 1107 20-21 0.2605519958591387 1107 22-23 0.2043231977577875 1107 24-25 0.15600882221989565 1107 26-27 0.17179062512505539 1107 28-29 0.2301044214562964 1107 30-31 0.24720412895417354 1107 32-33 0.22838942326884393 1107 34-35 0.26297023299189703 1107 36-37 0.1484955857133201 1107 38-39 0.2848609768164607 1107 40-41 0.21631547426287767 1107 42-43 0.2813097830614879 1107 44-45 0.2268000190215318 1107 46-47 0.29473097026155415 1107 48-49 0.2888173308317903 1107 50-51 0.25804463015894896 1107 52-53 0.44470008700653807 1107 54-55 0.29173866023604944 1107 56-57 0.19425897631776934 1107 58-59 0.17327002163172978 1107 60-61 0.11802372863319732 1107 62-63 0.166882850715929 1107 64-65 0.1588582084238439 1107 66-67 0.10564225923165793 1107 68-69 0.1800785477383684 1107 70-71 0.05206236171162715 1107 72-73 0.23156429547190527 1107 74-75 0.1988394467187682 1107 76-77 0.07303511242204408 1107 78-79 0.22695094119346848 1107 80-81 0.3172626892112973 1107 82-83 0.2514062126826566 1107 84-85 0.14826349372976821 1107 86-87 0.15806745512034936 1107 88-89 0.010163307340199879 1107 90-91 0.016917468213133446 1107 92-93 0.10548670800016424 1107 94-95 -0.22587103950670695 1107 96-97 -0.23296689869983211 1107 98-99 -0.435534947852652 1107 100-101 -0.7125071549889235 1108 1 0.18233168099085617 1108 2 0.17355246204272845 1108 3 0.1960960295969656 1108 4 0.17197941258438476 1108 5 0.10494782362266619 1108 6 0.1239456266809924 1108 7 0.13208306548272475 1108 8 0.1434069916316929 1108 9 0.22146131839949135 1108 10-11 0.1415122595507725 1108 12-13 0.20127655323728533 1108 14-15 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-0.2923634034361271 2316 70-71 -0.38575896483150274 2316 72-73 -0.32494307113212884 2316 74-75 -0.04334357613020501 2316 76-77 -0.21057647827869985 2316 78-79 -0.1293553375617904 2316 80-81 -0.19469112854273618 2316 82-83 0.0049557422794457295 2316 84-85 -0.010486921626448265 2316 86-87 0.009816815677442747 2316 88-89 0.17738838444712357 2316 90-91 0.006379134366525818 2316 92-93 0.017441670331066916 2316 94-95 -0.005762791149265922 2316 96-97 0.10743845319853662 2316 98-99 0.30905304560752 2316 100-101 0.32700428237428625 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6.0 3 16.0 4 54.0 5 107.0 6 242.0 7 666.0 8 1591.0 9 2742.0 10 4487.0 11 6877.0 12 9707.0 13 13345.0 14 17640.0 15 22348.0 16 28037.0 17 34263.0 18 33238.0 19 29983.0 20 30399.0 21 31852.0 22 34761.0 23 39347.0 24 45525.0 25 53764.0 26 63731.0 27 76879.0 28 92223.0 29 110045.0 30 130884.0 31 154883.0 32 187867.0 33 231029.0 34 293397.0 35 383056.0 36 501468.0 37 555922.0 38 269209.0 39 11630.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.10263247962791 19.01145210420533 15.18854624016506 23.697369176001708 2 16.078406173336646 20.045837367307247 39.19720349220765 24.67855296714846 3 19.57164755551369 23.205935161952393 29.69176664697033 27.530650635563585 4 13.6561220819703 16.701663041430457 35.69718715924207 33.94502771735717 5 14.19065036480897 36.843577422157566 34.14535959618874 14.820412616844724 6 33.615559399638045 35.74051872277504 16.48608994011224 14.157831937474667 7 29.506454062262716 31.874589663224118 19.95621171379474 18.662744560718426 8 26.73263454764474 35.34819394728279 19.011766317844724 18.90740518722775 9 26.027026564132427 16.720360125827096 19.507909865780626 37.744703444259855 10-11 25.355201785785646 26.25607298428303 27.034428325940134 21.354296903991184 12-13 26.277567495047414 23.185597821432854 27.329042994730564 23.20779168878917 14-15 22.60982753010088 24.959808404838977 25.278486649425385 27.151877415634758 16-17 22.713332305707322 28.059513818715352 26.3499865837715 22.877167291805826 18-19 22.746730151118115 26.627202973264595 28.299507310417273 22.326559565200014 20-21 22.98291286302316 25.870470595623456 28.69778375323274 22.448832788120644 22-23 22.835277259207242 25.750966253903552 28.606482036526398 22.807274450362808 24-25 22.911395909983206 25.973170914814485 28.503405302503733 22.612027872698576 26-27 22.349949906342918 26.377753991398933 28.330914173914273 22.941381928343873 28-29 22.547884517672312 26.73678786944582 28.170568790997997 22.544758821883867 30-31 22.586420493146306 26.283105257448863 28.074871118570915 23.05560313083392 32-33 22.306106953031783 26.202222526704006 28.14948818515537 23.342182335108845 34-35 23.079238756844198 26.350308482274066 27.969107095013797 22.601345665867942 36-37 23.174609155502996 25.83143971588388 27.99169791296025 23.002253215652875 38-39 22.599565326549605 26.21808549281998 27.983841120816354 23.198508059814056 40-41 23.11851216320199 26.024098174003164 28.106086546692016 22.751303116102832 42-43 22.840867975990577 26.06418546432091 27.871115256518493 23.223831303170023 44-45 22.787192925365808 26.358535861293326 28.084633565690993 22.769637647649876 46-47 23.316805681630044 26.54476453091727 27.343058671736287 22.7953711157164 48-49 22.91627417062017 26.674416679511992 27.242108688577936 23.167200461289898 50-51 23.13617186474158 26.809549500174125 27.257294717431392 22.796983917652902 52-53 23.50558914370208 26.41125878477515 27.28061611888491 22.80253595263786 54-55 22.973778409577474 26.68266623278013 27.467073149845 22.876482207797398 56-57 22.959805550322272 26.823079909340546 27.228421280992915 22.988693259344263 58-59 22.900131878671623 26.435036908900955 27.221213626320928 23.443617586106498 60-61 22.541690216429455 26.70009305724448 27.502982969953354 23.25523375637271 62-63 22.740421669207187 26.722558103687465 27.6201466079778 22.916873619127546 64-65 22.47348153984049 26.77114197795171 27.41814673357654 23.33722974863126 66-67 22.36482755362603 26.80692717084956 27.39966764857298 23.428577626951437 68-69 22.992577901208243 26.435571381145223 27.45529998826783 23.11655072937871 70-71 22.787348660574363 26.56965681267909 27.049743978250856 23.593250548495696 72-73 22.728418426476214 26.646870593339845 27.141900879762048 23.482810100421897 74-75 22.733142651617655 26.94997744931806 27.000188398102313 23.316691500961973 76-77 22.859754739924984 26.54386535815621 27.249387706167465 23.346992195751337 78-79 22.85160509474141 26.81483035607241 27.22682275163992 23.106741797546256 80-81 23.071884780877657 26.7518248123092 27.009934006885484 23.166356399927665 82-83 22.955993873019878 26.843246477357518 27.03822568775809 23.162533961864515 84-85 22.7754795368539 27.051263880259484 27.001892498276142 23.171364084610474 86-87 23.14798467030902 27.025352851651657 26.63274753924091 23.193914938798414 88-89 22.895508426472766 27.123738177111857 26.489192571795826 23.491560824619555 90-91 22.75610173846022 27.082983643059006 26.691287716593877 23.4696269018869 92-93 22.85450443989144 26.714223423986617 26.956688668089658 23.474583468032282 94-95 22.606956008421975 26.547492361304588 27.04573454791255 23.799817082360892 96-97 22.925037536894628 26.242985025205385 26.990497313899787 23.841480124000206 98-99 22.542987618812187 26.567002110630856 26.708586219869844 24.181424050687113 100-101 22.81071414298845 26.207275021266152 27.045832120163737 23.936178715581665 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 334.0 1 245.0 2 228.5 3 320.0 4 307.5 5 307.5 6 521.0 7 1230.5 8 1937.5 9 2164.0 10 1907.0 11 1616.0 12 1647.5 13 1733.5 14 1954.5 15 2182.5 16 2392.5 17 2727.5 18 3119.5 19 3520.0 20 4104.0 21 5056.5 22 6236.0 23 7792.5 24 9918.5 25 12646.5 26 16294.5 27 20953.0 28 25702.5 29 30366.5 30 35880.5 31 43904.5 32 56014.5 33 60937.5 34 59846.0 35 63468.5 36 69953.0 37 77596.0 38 84362.0 39 89671.5 40 95075.0 41 102629.0 42 108136.5 43 112644.5 44 118184.0 45 125983.5 46 130278.5 47 132816.0 48 134603.0 49 133189.0 50 133324.0 51 131785.0 52 130046.5 53 130839.0 54 132859.5 55 125442.0 56 113968.5 57 104676.5 58 96266.0 59 85035.5 60 68031.5 61 56517.5 62 48732.0 63 36414.5 64 25166.5 65 18931.0 66 14366.0 67 11303.0 68 8910.0 69 6993.5 70 5424.0 71 3699.5 72 2703.0 73 2156.5 74 1595.0 75 1178.5 76 869.0 77 574.5 78 356.0 79 238.5 80 140.5 81 77.5 82 58.0 83 33.5 84 14.0 85 6.5 86 6.5 87 4.5 88 2.0 89 1.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.994485073732166E-4 2 0.0488122356003905 3 8.563550105331666E-4 4 0.0 5 0.09045963427932018 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9981616912440555E-4 26-27 0.00803546451550288 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.00983381003762253 36-37 1.28453251579975E-4 38-39 0.003725144295819275 40-41 0.004981131644601252 42-43 3.5681458772215274E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.427258350888611E-5 68-69 9.419905115864833E-4 70-71 5.709033403554444E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014700761014152695 82-83 7.421743424620778E-4 84-85 0.005095312312672341 86-87 0.0023835214459839806 88-89 1.28453251579975E-4 90-91 0.0034254200421326665 92-93 0.0010704437631664583 94-95 1.1418066807108888E-4 96-97 0.0 98-99 5.709033403554444E-5 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3503220.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.40347379923917 #Duplication Level Percentage of deduplicated Percentage of total 1 72.17433435949783 36.37837170866477 2 15.12761172912491 15.249683628680211 3 5.393112370944444 8.154947942557529 4 2.4675529642835516 4.974929647340038 5 1.371595224428468 3.4566581978820925 6 0.8068467802898673 2.440072833020449 7 0.5564776967559104 1.9633886305808244 8 0.3666412118934013 1.478399257391228 9 0.27912682013299195 1.2662065228714385 >10 1.3105452456084066 11.685687648157515 >50 0.09205860582497725 3.1854901578057015 >100 0.047009447118541325 4.523298765249861 >500 0.003685522938899768 1.2284660579117705 >1k 0.0032886204525301326 3.5957460016899203 >5k 1.1340070525965975E-4 0.41865300019675755 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7870 0.22465046442986739 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6779 0.19350768721347789 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4946 0.1411843960699014 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4881 0.13932896021374622 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4678 0.13353429130913844 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4646 0.13262084596456974 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4574 0.13056559393929013 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4187 0.11951861430341229 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3845 0.1097561671833342 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3614 0.10316223360222881 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.854516701777222E-5 0.0 0.0 0.0 1.8554358561551944E-4 14-15 5.709033403554444E-5 0.0 0.0 0.0 2.4263391965106387E-4 16-17 9.990808456220277E-5 0.0 0.0 0.0 2.5690650315995E-4 18-19 1.1418066807108888E-4 0.0 0.0 0.0 2.5690650315995E-4 20-21 1.427258350888611E-4 0.0 0.0 7.136291754443056E-5 3.1399683719549444E-4 22-23 1.7127100210663333E-4 0.0 0.0 1.141806680710889E-4 3.4254200421326666E-4 24-25 1.7127100210663333E-4 0.0 0.0 1.9981616912440555E-4 3.4254200421326666E-4 26-27 1.7127100210663333E-4 0.0 0.0 5.423581733376722E-4 3.85359754739925E-4 28-29 1.7127100210663333E-4 0.0 0.0 0.002040979441770714 3.996323382488111E-4 30-31 1.7127100210663333E-4 0.0 0.0 0.008663458189893869 3.996323382488111E-4 32-33 1.7127100210663333E-4 0.0 0.0 0.02289322394825332 4.281775052665833E-4 34-35 1.8554358561551944E-4 0.0 0.0 0.044773094467375735 4.281775052665833E-4 36-37 1.9981616912440555E-4 0.0 0.0 0.08139654375117748 4.4245008877546945E-4 38-39 1.9981616912440555E-4 0.0 0.0 0.13534690941476699 4.995404228110139E-4 40-41 1.9981616912440555E-4 0.0 0.0 0.19273696770399804 5.138130063199E-4 42-43 1.9981616912440555E-4 0.0 0.0 0.25569333356169466 5.138130063199E-4 44-45 1.9981616912440555E-4 0.0 0.0 0.336233522302339 5.28085589828786E-4 46-47 1.9981616912440555E-4 0.0 0.0 0.43470007593014426 5.709033403554443E-4 48-49 1.9981616912440555E-4 0.0 0.0 0.5352361541667381 7.279017589531916E-4 50-51 1.9981616912440555E-4 0.0 0.0 0.6478040203013228 7.992646764976222E-4 52-53 1.9981616912440555E-4 0.0 0.0 0.7548483966179687 8.563550105331666E-4 54-55 1.9981616912440555E-4 0.0 0.0 0.8749093690947186 8.563550105331666E-4 56-57 1.9981616912440555E-4 0.0 0.0 1.0173069347628754 8.563550105331666E-4 58-59 1.9981616912440555E-4 0.0 0.0 1.1675686939444283 8.563550105331666E-4 60-61 1.9981616912440555E-4 0.0 0.0 1.3352001872562957 8.563550105331666E-4 62-63 1.9981616912440555E-4 0.0 0.0 1.5002768881200725 9.13445344568711E-4 64-65 1.9981616912440555E-4 0.0 0.0 1.677542375300438 9.13445344568711E-4 66-67 1.9981616912440555E-4 0.0 0.0 1.8823111309024267 9.419905115864833E-4 68-69 1.9981616912440555E-4 0.0 0.0 2.1084173988502006 9.419905115864833E-4 70-71 2.1408875263329166E-4 0.0 0.0 2.346869451533161 9.562630950953695E-4 72-73 2.2836133614217776E-4 0.0 0.0 2.5998367216446585 0.0010847163466753444 74-75 2.2836133614217776E-4 0.0 0.0 2.857185674893384 0.0011988970147464332 76-77 2.2836133614217776E-4 0.0 0.0 3.135229874229994 0.0011988970147464332 78-79 2.2836133614217776E-4 0.0 0.0 3.4552925594167654 0.0012131695982553195 80-81 2.2836133614217776E-4 0.0 0.0 3.795536677685101 0.0012274421817642055 82-83 2.2836133614217776E-4 0.0 1.427258350888611E-5 4.152037268570059 0.001313077682817522 84-85 2.2836133614217776E-4 0.0 2.854516701777222E-5 4.516958683725258 0.001313077682817522 86-87 2.2836133614217776E-4 0.0 2.854516701777222E-5 4.889330387472097 0.0013558954333441806 88-89 2.2836133614217776E-4 0.0 2.854516701777222E-5 5.292759232934272 0.001398713183870839 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5085 0.0 54.808693 1 GTATCAA 8790 0.0 41.013153 1 TATCAAC 11595 0.0 30.926958 2 ATCAACG 11585 0.0 30.896704 3 TCAACGC 11825 0.0 30.189226 4 CAACGCA 12095 0.0 29.554605 5 AACGCAG 12620 0.0 28.45221 6 ACGCAGA 14475 0.0 24.641941 7 CGCAGAG 14625 0.0 24.389202 8 TAAGGTG 2320 0.0 24.177267 5 TGGTATC 2400 0.0 23.779715 2 GGACCGA 980 0.0 23.263159 6 AGGTAAG 2220 0.0 22.494324 2 GTAAGGT 2360 0.0 22.35755 4 GTGGTAT 2885 0.0 21.59575 1 AGGACCG 1305 0.0 21.490904 5 TACATGG 9895 0.0 20.859818 2 TAGTCCG 160 4.964808E-5 20.79655 5 GCAGAGT 16850 0.0 20.689487 9 GTACATG 10215 0.0 20.532557 1 >>END_MODULE