FastQCFastQC Report
Thu 26 May 2016
SRR938529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500692
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83920.23972403170573134No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64130.18319235168360998No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA50220.14345735071808657No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48410.13828694440984812No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45370.12960294707446413No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42830.12234723877450515No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA42820.12231867299379666No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA38020.1086070982537167No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36800.1051220730072797No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47500.052.6848871
GTATCAA89200.045.4763031
ATCAACG116150.034.533283
TCAACGC116850.034.3264054
TATCAAC116850.034.2847562
CAACGCA119650.033.562835
AACGCAG125250.032.1325956
ACGCAGA141750.027.9509247
CGCAGAG143550.027.5673488
TAAGGTG22450.026.2494355
GTAAGGT21700.024.5286064
TGGTATC22350.023.6019342
GCAGAGT163300.023.592589
AGGTAAG21050.023.2534472
TACATGG100200.022.1488572
GTACATG103000.022.0287631
GAGTACT98900.021.78118112-13
GTGGTAT27150.021.5951211
GGTAAGG24950.021.143023
AGAGTAC143750.021.04992910-11
Produced by FastQC (version 0.11.5)