FastQCFastQC Report
Thu 26 May 2016
SRR938529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3500692
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79140.22606958852706838No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68610.19598982144101795No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA48470.1384583390940991No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48110.1374299709885931No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47140.13465909025986864No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46690.13337363012798611No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45190.12908876302171116No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42810.12229010721308815No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG37170.10617900689349419No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36390.1039508759982312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52450.051.5056841
GTATCAA90150.041.3621061
ATCAACG116350.031.7887173
TATCAAC117750.031.3439062
TCAACGC118400.031.198174
CAACGCA122200.030.189115
AACGCAG127750.028.9242886
TAAGGTG22150.028.760135
GTAAGGT20950.028.3651944
ACGCAGA146350.025.1508667
CGCAGAG148100.024.7895378
AGGTAAG24200.022.7979052
GGTAAGG26000.022.4901073
TGGTATC26400.022.159172
GTACATG106300.021.5658261
GCAGAGT170550.021.1643949
TACATGG104800.020.8306922
GTGGTAT31050.020.5255831
AAGGTAA24800.020.5203321
AGAGTAC146100.020.35017210-11
Produced by FastQC (version 0.11.5)