##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3500692 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.795971196551996 31.0 31.0 34.0 30.0 34.0 2 32.02654332343434 31.0 31.0 34.0 30.0 34.0 3 32.101134861335986 33.0 31.0 34.0 30.0 34.0 4 35.770897011219496 37.0 35.0 37.0 35.0 37.0 5 35.63637360841799 37.0 35.0 37.0 33.0 37.0 6 35.67443208371373 37.0 35.0 37.0 33.0 37.0 7 35.60722879933453 37.0 35.0 37.0 33.0 37.0 8 35.585637925301626 37.0 35.0 37.0 33.0 37.0 9 37.16517077195023 39.0 37.0 39.0 33.0 39.0 10-11 37.09310744847019 39.0 37.0 39.0 33.0 39.0 12-13 36.98488513128262 39.0 37.0 39.0 33.0 39.0 14-15 38.04202883315642 40.0 37.0 41.0 33.0 41.0 16-17 37.95271477753541 40.0 37.0 41.0 33.0 41.0 18-19 37.94091253957789 40.0 37.0 41.0 33.0 41.0 20-21 37.923224465334286 40.0 37.0 41.0 32.5 41.0 22-23 37.95783290846496 40.0 37.0 41.0 33.0 41.0 24-25 37.88376855204628 40.0 37.0 41.0 32.5 41.0 26-27 37.70359474641014 40.0 37.0 41.0 32.0 41.0 28-29 37.57093697474671 39.5 37.0 41.0 32.0 41.0 30-31 37.32316710524662 39.0 36.0 41.0 31.0 41.0 32-33 37.10431094766406 39.0 36.0 41.0 31.0 41.0 34-35 36.943566014947905 39.0 36.0 40.5 30.5 41.0 36-37 36.953073420912204 39.0 36.0 41.0 30.5 41.0 38-39 36.802679013177965 39.0 35.0 41.0 30.0 41.0 40-41 36.620010843570356 39.0 35.0 40.5 30.0 41.0 42-43 36.39521443188947 39.0 35.0 40.0 30.0 41.0 44-45 36.12425271917667 38.5 35.0 40.0 29.5 41.0 46-47 35.787692976131574 38.0 35.0 40.0 28.5 41.0 48-49 35.39168084481582 38.0 34.0 40.0 27.0 41.0 50-51 35.01545537282343 38.0 34.0 40.0 26.5 41.0 52-53 34.48263800414318 38.0 33.0 40.0 24.5 41.0 54-55 34.14178382445528 37.0 33.0 40.0 24.0 41.0 56-57 33.764003231361116 37.0 33.0 40.0 23.0 41.0 58-59 33.28762870312498 36.5 32.0 39.0 22.5 41.0 60-61 32.865173085778466 36.0 31.0 39.0 21.5 40.0 62-63 32.36792054256701 35.5 31.0 39.0 19.5 40.0 64-65 31.857591870407337 35.0 30.5 38.0 18.0 40.0 66-67 31.348707484120283 35.0 30.0 38.0 15.5 40.0 68-69 30.768485202354277 34.0 29.0 37.5 12.0 39.5 70-71 30.181344145671773 34.0 29.0 37.0 8.0 39.0 72-73 29.58801088470508 34.0 28.0 36.0 7.0 39.0 74-75 28.961845115194368 33.5 27.0 35.5 4.0 38.5 76-77 27.701900795614122 31.5 26.0 34.5 2.0 36.0 78-79 28.322181157325467 33.0 27.0 35.0 2.0 37.0 80-81 28.027615254355425 33.0 26.0 35.0 2.0 36.5 82-83 27.5294568902377 33.0 26.0 35.0 2.0 36.0 84-85 26.929911857427044 32.0 25.0 35.0 2.0 36.0 86-87 26.281138986234723 32.0 24.5 34.5 2.0 35.0 88-89 25.51348090606086 31.0 21.0 34.0 2.0 35.0 90-91 24.958832425131945 31.0 19.5 34.0 2.0 35.0 92-93 24.178386016250503 31.0 15.0 34.0 2.0 35.0 94-95 23.380165121638807 30.5 4.5 34.0 2.0 35.0 96-97 22.393041290122067 30.0 2.0 34.0 2.0 35.0 98-99 21.207672940092987 29.5 2.0 34.0 2.0 35.0 100-101 18.088479220679794 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3291346453179962 1101 2 0.3403149191979722 1101 3 0.3614361552556318 1101 4 0.246627254244828 1101 5 0.19303116364761053 1101 6 0.17550073670228272 1101 7 0.256306231915282 1101 8 0.28589249731010113 1101 9 0.24750930182591446 1101 10-11 0.3671610872348907 1101 12-13 0.39770143449057116 1101 14-15 0.53505644824466 1101 16-17 0.5585966711171366 1101 18-19 0.6056387745142473 1101 20-21 0.46263378491276086 1101 22-23 0.48767549259370924 1101 24-25 0.527552956666419 1101 26-27 0.502975409534514 1101 28-29 0.4435369354167875 1101 30-31 0.4425296500302309 1101 32-33 0.4547395949627102 1101 34-35 0.29045165922577354 1101 36-37 0.5411808723368807 1101 38-39 0.5894936515786782 1101 40-41 0.7530483378208928 1101 42-43 0.6889505712156634 1101 44-45 0.7684642221770019 1101 46-47 0.8164082530590306 1101 48-49 0.8446363492552962 1101 50-51 0.9255271413538608 1101 52-53 0.9755841295988645 1101 54-55 1.129610366061435 1101 56-57 1.1680707980948952 1101 58-59 1.1227151449771853 1101 60-61 1.2128551764748678 1101 62-63 1.2501975639882161 1101 64-65 1.3834219758961304 1101 66-67 1.413381125636409 1101 68-69 1.5484979781591335 1101 70-71 1.6347645793856778 1101 72-73 1.6458989317130346 1101 74-75 1.803781678241247 1101 76-77 1.6433132006687998 1101 78-79 2.042655506454448 1101 80-81 2.2721469607190947 1101 82-83 2.2733640476913877 1101 84-85 2.5214573956749184 1101 86-87 2.5073253542773486 1101 88-89 2.63078570295016 1101 90-91 2.9959381893770676 1101 92-93 3.3243718100253012 1101 94-95 3.515308930178609 1101 96-97 3.7219220628323946 1101 98-99 4.078385149338345 1101 100-101 4.3735773892605 1102 1 0.3321261379174736 1102 2 0.34798020685713027 1102 3 0.315639319585145 1102 4 0.24473375958727672 1102 5 0.2225324057068434 1102 6 0.2127626967197358 1102 7 0.2684517065225833 1102 8 0.30904167153578754 1102 9 0.4030237810384065 1102 10-11 0.3571515753434724 1102 12-13 0.43137537095560674 1102 14-15 0.540552390900018 1102 16-17 0.49780367830206274 1102 18-19 0.5289528361779787 1102 20-21 0.5551161394658095 1102 22-23 0.5137685358531172 1102 24-25 0.45426010580621323 1102 26-27 0.4842757416905741 1102 28-29 0.4167093444475256 1102 30-31 0.3791989622470311 1102 32-33 0.2760611049521202 1102 34-35 0.42673166710930843 1102 36-37 0.4549240626248974 1102 38-39 0.5505148162239664 1102 40-41 0.5011842873486998 1102 42-43 0.4910245133955229 1102 44-45 0.4119432646942158 1102 46-47 0.48294964837822363 1102 48-49 0.5307578988821717 1102 50-51 0.7444653666287095 1102 52-53 0.8191955747872584 1102 54-55 0.7521410841771754 1102 56-57 0.9011854049385519 1102 58-59 0.9833692326726933 1102 60-61 1.028728550542219 1102 62-63 1.0832581455598742 1102 64-65 1.0821475143263335 1102 66-67 1.0179212175352284 1102 68-69 1.2011995531437165 1102 70-71 1.405548281942071 1102 72-73 1.3924644483682798 1102 74-75 1.3651850769519704 1102 76-77 1.2918265120049313 1102 78-79 1.7610846083362723 1102 80-81 1.9224274268878645 1102 82-83 1.994258355979241 1102 84-85 2.1879073445894868 1102 86-87 2.253218417025792 1102 88-89 2.4960613371586753 1102 90-91 2.5584252989599925 1102 92-93 2.690072072431146 1102 94-95 2.8011355433994254 1102 96-97 3.238790650365061 1102 98-99 3.6333273581413046 1102 100-101 4.029438558873299 1103 1 0.22161577811317912 1103 2 0.3008755159241545 1103 3 0.28993941698259107 1103 4 0.24080848664791432 1103 5 0.11827926577161918 1103 6 0.16401937863778215 1103 7 0.23496673234423326 1103 8 0.16853240179015216 1103 9 0.32925264522559416 1103 10-11 0.37924596734556104 1103 12-13 0.435869739457722 1103 14-15 0.5280529925557786 1103 16-17 0.5442465479027376 1103 18-19 0.501492559614519 1103 20-21 0.4992969957705995 1103 22-23 0.42225864180115735 1103 24-25 0.48214207227204753 1103 26-27 0.40828555788119303 1103 28-29 0.4186666721899499 1103 30-31 0.510084040334867 1103 32-33 0.503525380756777 1103 34-35 0.5109693126392187 1103 36-37 0.6050644253737687 1103 38-39 0.6544276667415261 1103 40-41 0.687952567774083 1103 42-43 0.7032821169086247 1103 44-45 0.7189804073814798 1103 46-47 0.6883949161079599 1103 48-49 0.7750906555697483 1103 50-51 0.896385037799071 1103 52-53 1.1057435076396445 1103 54-55 1.046534254181033 1103 56-57 1.173483829025166 1103 58-59 1.307952780714551 1103 60-61 1.2702032654281723 1103 62-63 1.408763996485142 1103 64-65 1.3085541147023356 1103 66-67 1.319859858557681 1103 68-69 1.366597728923466 1103 70-71 1.4335231511350308 1103 72-73 1.435518757356025 1103 74-75 1.479146463393338 1103 76-77 1.3058739352194983 1103 78-79 1.7188731482805544 1103 80-81 2.00972183757667 1103 82-83 2.1622056056338224 1103 84-85 2.332739510946592 1103 86-87 2.477020378848259 1103 88-89 2.8499849820883476 1103 90-91 2.9803470157599214 1103 92-93 3.166642535522044 1103 94-95 3.1747377555106873 1103 96-97 3.5688678463377883 1103 98-99 3.9482943237011128 1103 100-101 4.377505792842353 1104 1 0.2830634806516663 1104 2 0.25530469265030575 1104 3 0.22408517156515018 1104 4 0.18777227450878087 1104 5 0.1853997127892626 1104 6 0.17590172833693885 1104 7 0.1957489292019048 1104 8 0.23547599562164834 1104 9 0.24828354353559945 1104 10-11 0.2554660022826738 1104 12-13 0.2601007767634087 1104 14-15 0.3389425363568961 1104 16-17 0.4011809739339114 1104 18-19 0.4558469623094794 1104 20-21 0.4835647916539543 1104 22-23 0.4075389536811258 1104 24-25 0.44350864078588614 1104 26-27 0.48692943924825016 1104 28-29 0.4775203459641233 1104 30-31 0.5174116853527977 1104 32-33 0.5959025211424205 1104 34-35 0.7222049005748232 1104 36-37 0.7285246684414588 1104 38-39 0.7276289648187131 1104 40-41 0.7543698448432892 1104 42-43 0.8970047025643808 1104 44-45 0.8924324253057421 1104 46-47 0.8397300778483867 1104 48-49 1.0045727454249516 1104 50-51 0.9813545081502397 1104 52-53 1.1644045332637774 1104 54-55 1.242427723198702 1104 56-57 1.4089873287858907 1104 58-59 1.47589924553278 1104 60-61 1.4526292593010695 1104 62-63 1.5076644569587572 1104 64-65 1.461390890217583 1104 66-67 1.5353531974517303 1104 68-69 1.6302435275302827 1104 70-71 1.7306986755278366 1104 72-73 1.8638504949499222 1104 74-75 2.130550770108684 1104 76-77 1.9709271491249822 1104 78-79 2.139608474423035 1104 80-81 2.4551227221618603 1104 82-83 2.480763730428823 1104 84-85 2.5983443891707836 1104 86-87 2.7448120194140273 1104 88-89 2.8379041871513415 1104 90-91 3.0657044289077824 1104 92-93 3.3921822696020634 1104 94-95 3.553718380467 1104 96-97 3.895604401798945 1104 98-99 4.31588946819479 1104 100-101 4.5114277210243365 1105 1 0.2795346665463221 1105 2 0.26257799548169913 1105 3 0.25203222176705253 1105 4 0.17457815176209834 1105 5 0.17555246365989774 1105 6 0.17457850298613664 1105 7 0.23305488299654087 1105 8 0.21362075422075577 1105 9 0.20696962304982947 1105 10-11 0.2891104037556218 1105 12-13 0.327567803047657 1105 14-15 0.46018200992855895 1105 16-17 0.43112285449989685 1105 18-19 0.4065692684451321 1105 20-21 0.3946702359992358 1105 22-23 0.36377291348074436 1105 24-25 0.32894276989568993 1105 26-27 0.47988220306894647 1105 28-29 0.5286258782419679 1105 30-31 0.4883863819554435 1105 32-33 0.5693042367069268 1105 34-35 0.6713415026283727 1105 36-37 0.7337428271573572 1105 38-39 0.6162654783401393 1105 40-41 0.5675082503139706 1105 42-43 0.8074612844573821 1105 44-45 0.7679531160400899 1105 46-47 0.8816653618725994 1105 48-49 0.928684376626137 1105 50-51 1.0391735724916984 1105 52-53 1.0316904184470204 1105 54-55 1.012746602681915 1105 56-57 1.0986994175562188 1105 58-59 1.2132082369981205 1105 60-61 1.2392973222304207 1105 62-63 1.309265589210547 1105 64-65 1.5259169716183614 1105 66-67 1.4961445826175925 1105 68-69 1.534844399581587 1105 70-71 1.6537158659462214 1105 72-73 1.793193154998196 1105 74-75 1.8414977759446103 1105 76-77 1.6910232594276025 1105 78-79 1.9997768173376862 1105 80-81 2.2703162317786862 1105 82-83 2.2803675542167454 1105 84-85 2.3817482750710752 1105 86-87 2.553011532027753 1105 88-89 2.743897231684791 1105 90-91 2.7987592025073873 1105 92-93 3.1979906957009305 1105 94-95 3.2482008912777083 1105 96-97 3.635538620240247 1105 98-99 4.175212499294542 1105 100-101 4.411888607616493 1106 1 0.15159838066724518 1106 2 0.20626230038887172 1106 3 0.14727104085959297 1106 4 0.11161980274290073 1106 5 0.11088867963047022 1106 6 0.13554857652466268 1106 7 0.12509580361950867 1106 8 0.11668505064865542 1106 9 0.1508698288563508 1106 10-11 0.2794924422575562 1106 12-13 0.13916149133093825 1106 14-15 0.07718109826781472 1106 16-17 0.16649414348051295 1106 18-19 0.2502405892131492 1106 20-21 0.4748408032517304 1106 22-23 0.31912073600499014 1106 24-25 0.29109780447586076 1106 26-27 0.2998694643616844 1106 28-29 0.38587334366279435 1106 30-31 0.34001381316623025 1106 32-33 0.2854665606144877 1106 34-35 0.22455786786903786 1106 36-37 0.29377653841622475 1106 38-39 0.25282770581672054 1106 40-41 0.3210229340690418 1106 42-43 0.5062767192736359 1106 44-45 0.0679135698357527 1106 46-47 -0.11512569522238181 1106 48-49 0.3075119959486017 1106 50-51 0.10025721766209728 1106 52-53 0.4491245150339722 1106 54-55 0.44608559678324156 1106 56-57 0.3841294716422894 1106 58-59 0.8598667410748675 1106 60-61 0.6667841114626043 1106 62-63 0.7525894259128236 1106 64-65 0.4706455892548611 1106 66-67 0.6448574704445242 1106 68-69 0.4262581625315889 1106 70-71 0.6606355412319012 1106 72-73 1.0596673159674808 1106 74-75 1.217814824871457 1106 76-77 1.309259522465677 1106 78-79 1.6153100834543501 1106 80-81 1.6023980603571708 1106 82-83 1.8035530306653733 1106 84-85 1.7738684860316702 1106 86-87 2.1280267230747114 1106 88-89 2.4030699915942044 1106 90-91 2.240130707626424 1106 92-93 2.062629223996261 1106 94-95 2.5955827464974313 1106 96-97 3.0816625578071246 1106 98-99 3.248117728141974 1106 100-101 3.6882394628081094 1107 1 0.16491016061746677 1107 2 0.10606939294478934 1107 3 0.1252725943826647 1107 4 0.1062960704620437 1107 5 0.0830248671542293 1107 6 -0.03734991030654555 1107 7 0.0805309183726095 1107 8 0.12081844836222189 1107 9 0.1093550738861282 1107 10-11 0.1727501288144495 1107 12-13 0.18786747192822162 1107 14-15 0.23247345684706033 1107 16-17 0.32123892016740996 1107 18-19 0.2521517025013651 1107 20-21 0.3929311828385309 1107 22-23 0.2722962444572232 1107 24-25 0.2924352105588568 1107 26-27 0.28251222636708206 1107 28-29 0.33590355105579306 1107 30-31 0.3497808313583093 1107 32-33 0.32800568443681044 1107 34-35 0.29199686409719305 1107 36-37 0.4733000080443901 1107 38-39 0.46362212526328506 1107 40-41 0.46546512365016923 1107 42-43 0.4721970650608185 1107 44-45 0.47349540621198116 1107 46-47 0.492703384583443 1107 48-49 0.5794468786062907 1107 50-51 0.367632650071009 1107 52-53 0.5103720453754903 1107 54-55 0.3561875788080897 1107 56-57 0.4730844124754512 1107 58-59 0.626754189961396 1107 60-61 0.7360908342987003 1107 62-63 0.8362908462434504 1107 64-65 0.81541387872333 1107 66-67 1.0344887536405842 1107 68-69 1.087040335737747 1107 70-71 1.0553115756893234 1107 72-73 1.2137270029768992 1107 74-75 1.1890004708781348 1107 76-77 1.168756540214563 1107 78-79 1.4502810307068756 1107 80-81 1.6794411414646042 1107 82-83 1.7855704179941299 1107 84-85 1.969898488154346 1107 86-87 2.2156821628547014 1107 88-89 2.370040598284614 1107 90-91 2.3395098815533615 1107 92-93 2.643483685010061 1107 94-95 2.8175684802105145 1107 96-97 3.166854520541232 1107 98-99 3.4263055411500467 1107 100-101 3.6129001253180526 1108 1 0.1953454757738804 1108 2 0.2008802189233947 1108 3 0.17497205833949891 1108 4 0.1681227594879644 1108 5 0.1058230703963261 1108 6 0.06269151222329583 1108 7 0.11778157589959903 1108 8 0.17009789677447174 1108 9 0.23104043636666916 1108 10-11 0.1921279668701672 1108 12-13 0.26268012406137586 1108 14-15 0.3957292231527276 1108 16-17 0.39881853048384386 1108 18-19 0.3911775158639159 1108 20-21 0.4185859817276949 1108 22-23 0.3392706849746929 1108 24-25 0.3193443771865532 1108 26-27 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0.6653261382606281 2316 72-73 0.7708579987473136 2316 74-75 0.9066263645661827 2316 76-77 0.7314189439790653 2316 78-79 0.9183451341536966 2316 80-81 1.1138787722741768 2316 82-83 1.2440405854525949 2316 84-85 1.3877586637788113 2316 86-87 1.6024301119160569 2316 88-89 1.8027150252396602 2316 90-91 2.0028763526951145 2316 92-93 1.7303300998560367 2316 94-95 2.4585826259680097 2316 96-97 2.4539003841268787 2316 98-99 2.7201258507175865 2316 100-101 3.1022171059276964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 24.0 4 53.0 5 103.0 6 247.0 7 697.0 8 1514.0 9 2788.0 10 4562.0 11 7025.0 12 9858.0 13 13715.0 14 18023.0 15 23916.0 16 29765.0 17 36364.0 18 35821.0 19 33359.0 20 34146.0 21 36178.0 22 40179.0 23 44648.0 24 51761.0 25 59693.0 26 70139.0 27 84120.0 28 99236.0 29 117379.0 30 138357.0 31 163353.0 32 194835.0 33 235271.0 34 292524.0 35 369190.0 36 464541.0 37 506488.0 38 265962.0 39 14854.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.17818710648841 18.982909374177844 15.139982363481952 23.698921155851803 2 16.094788566477114 20.059706542856276 39.1791464925121 24.666358398154504 3 19.59192581341891 23.199731938330963 29.678374559050834 27.529967689199292 4 13.681209315186827 16.65699238893339 35.712996173328015 33.94880212255177 5 14.179060518751765 36.84599864347226 34.15172980163355 14.823211036142428 6 33.627922707853195 35.680631143785284 16.497223977430746 14.194222170930775 7 29.50796585360837 31.908291274982204 19.938686408287275 18.64505646312215 8 26.72448761559143 35.37860514435431 18.99558715819615 18.9013200818581 9 26.060447477241645 16.743089651988807 19.484861850171338 37.71160102059821 10-11 25.349488044078143 26.23467017378278 27.03156975820781 21.38427202393127 12-13 26.28795963769449 23.22423680803681 27.30057371513975 23.18722983912895 14-15 22.60525918875468 24.94366828044284 25.286414800273775 27.164657730528706 16-17 22.688157084370747 28.038456396621008 26.36768673165191 22.90569978735633 18-19 22.811418428127926 26.61979402929478 28.298062211699857 22.27072533087744 20-21 22.97048697800321 25.870613581543306 28.73277626252181 22.42612317793168 22-23 22.82280189174026 25.73753989211276 28.61808465297718 22.821573563169796 24-25 22.931773359050418 25.975560009323967 28.475891375787853 22.616775255837762 26-27 22.35059868516815 26.353189323587106 28.34110720080633 22.95510479043841 28-29 22.55808280191459 26.729586607447896 28.21119367256531 22.5011369180722 30-31 22.587891194083912 26.29558670114366 28.08009102200365 23.03643108276878 32-33 22.30898315598443 26.178174837199464 28.175287112116376 23.33755489469973 34-35 23.101052677591756 26.36490889941187 27.945146656469067 22.588891766527308 36-37 23.204528947599584 25.814629951929568 27.971736213226762 23.00910488724409 38-39 22.606139950532423 26.183689984136947 28.027460719129728 23.1827093462009 40-41 23.104408597251005 26.003654678712945 28.125170960430886 22.766765763605164 42-43 22.858214536493783 26.05894448643828 27.857166119032804 23.225674858035134 44-45 22.79007957360398 26.331791262372363 28.077308560786303 22.800820603237362 46-47 23.29232902437206 26.573983007345127 27.324035354183135 22.809652614099683 48-49 22.933222917068967 26.655858327439258 27.269565560180673 23.141353195311098 50-51 23.15299375094981 26.788889168198743 27.269679823303505 22.788437257547937 52-53 23.482257222286336 26.40400812182277 27.316413440542615 22.79732121534828 54-55 22.990425892937736 26.70007815597602 27.4353327856321 22.874163165454146 56-57 23.00719400621363 26.842007237426202 27.214147945606182 22.93665081075399 58-59 22.93962165194767 26.407550278630627 27.213233840623513 23.439594228798192 60-61 22.572437106720614 26.702591944678367 27.499234437077014 23.225736511524005 62-63 22.731234281679168 26.745469181521823 27.59668659796406 22.92660993883495 64-65 22.487239665757514 26.782904637140316 27.396726133004563 23.333129564097614 66-67 22.411846933451482 26.77729068284178 27.38087229461993 23.42999008908681 68-69 23.029786161912266 26.431405158874576 27.438779142145282 23.10002953706788 70-71 22.799920701392754 26.56469063830808 27.054879435265946 23.58050922503322 72-73 22.765687864811852 26.640436610881025 27.104116429568272 23.489759094738854 74-75 22.756143642456976 26.975195189979583 26.9317323546316 23.33692881293184 76-77 22.85632383540169 26.560620014557124 27.221046581647286 23.36200956839391 78-79 22.876619822595075 26.821954059368835 27.192737892965162 23.108688225070928 80-81 23.088562540527725 26.754925457552652 26.990464736951857 23.166047264967762 82-83 22.938331371556767 26.880509596279666 27.047107061581503 23.134051970582064 84-85 22.835475575468685 27.00136379036594 26.973753748709484 23.18940688545589 86-87 23.176536559252206 26.99775984204058 26.652494996637767 23.173208602069447 88-89 22.921306525098405 27.12743385912008 26.47136329247044 23.479896323311074 90-91 22.788689682122982 27.04179952249032 26.71013368328619 23.459377112100512 92-93 22.924599042758775 26.698146219954094 26.927772707341564 23.449482029945568 94-95 22.661091007149444 26.53339682554192 27.019729242104134 23.7857829252045 96-97 22.9047713994833 26.257822739047022 26.979879978015774 23.8575258834539 98-99 22.642537532579272 26.564590658075605 26.68609520631921 24.106776603025917 100-101 22.84800949305075 26.19083054481633 27.0236720669724 23.93748789516052 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 342.0 1 256.5 2 241.0 3 335.0 4 307.0 5 302.5 6 537.5 7 1228.5 8 1977.0 9 2290.0 10 2048.5 11 1672.0 12 1681.0 13 1842.5 14 2021.5 15 2314.0 16 2540.5 17 2755.0 18 3193.0 19 3605.0 20 4161.5 21 5081.5 22 6341.0 23 7842.5 24 9863.5 25 12888.0 26 16541.5 27 21091.0 28 25791.0 29 30348.5 30 35949.5 31 44357.5 32 55950.0 33 60097.5 34 59314.5 35 63342.0 36 69878.0 37 77318.5 38 84402.0 39 89751.0 40 94792.5 41 102156.5 42 107600.0 43 112118.0 44 118105.0 45 126029.5 46 130062.0 47 132300.5 48 133645.0 49 132213.0 50 132137.0 51 130872.0 52 129694.0 53 130341.0 54 132212.0 55 124976.0 56 113810.0 57 104885.0 58 96540.5 59 85161.0 60 68471.0 61 57253.5 62 49023.5 63 36760.0 64 25861.5 65 19653.0 66 14985.5 67 11625.0 68 9023.0 69 6992.5 70 5500.5 71 3873.0 72 2844.0 73 2277.0 74 1681.5 75 1218.0 76 853.5 77 555.0 78 380.5 79 235.5 80 137.5 81 83.0 82 47.0 83 35.0 84 22.5 85 13.0 86 5.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 2.8565780708499918E-5 2 0.04507680195801288 3 1.428289035424996E-4 4 0.0 5 0.1007800743395877 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0010283681055059972 26-27 0.011326332050920217 28-29 0.0 30-31 0.0 32-33 3.57072258856249E-4 34-35 0.014925620420191209 36-37 0.002842295180495742 38-39 0.011983345007215715 40-41 0.007070030725353731 42-43 0.005084708966112986 44-45 0.002756597838370242 46-47 8.569734212549977E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.5711179389674956E-4 68-69 1.7139468425099954E-4 70-71 0.0 72-73 1.4282890354249959E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.7131561416999836E-5 82-83 0.001099782557277247 84-85 0.004399130229108988 86-87 0.0013140259125909964 88-89 4.2848671062749884E-5 90-91 8.569734212549975E-4 92-93 1.9996046495949943E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.427102984209979E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3500692.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.53294656037867 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29962785681161 36.535132308235255 2 14.999835958032087 15.159718177633636 3 5.387833320743487 8.167892798200745 4 2.514333213217707 5.082266635940623 5 1.3662446928011196 3.452018502485997 6 0.8189810437996851 2.483131519217557 7 0.5267615555973678 1.8633169477344607 8 0.3755855102274851 1.5183554013742446 9 0.2572403845181274 1.1699203143623211 >10 1.3111565510292291 11.713144281028654 >50 0.0885956754587602 3.093654747183942 >100 0.046840781232308094 4.518148253287477 >500 0.0034534215400647845 1.1490871407211019 >1k 0.0033968080558974753 3.6715230746813328 >5k 1.1322693519658249E-4 0.4226898979126572 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7914 0.22606958852706838 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6861 0.19598982144101795 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4847 0.1384583390940991 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4811 0.1374299709885931 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4714 0.13465909025986864 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4669 0.13337363012798611 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4519 0.12908876302171116 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4281 0.12229010721308815 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3717 0.10617900689349419 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3639 0.1039508759982312 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 2.8565780708499918E-5 0.0 3 0.0 0.0 0.0 2.8565780708499918E-5 0.0 4 0.0 0.0 0.0 2.8565780708499918E-5 0.0 5 0.0 0.0 0.0 2.8565780708499918E-5 5.7131561416999836E-5 6 0.0 0.0 0.0 2.8565780708499918E-5 5.7131561416999836E-5 7 0.0 0.0 0.0 2.8565780708499918E-5 5.7131561416999836E-5 8 0.0 0.0 0.0 2.8565780708499918E-5 5.7131561416999836E-5 9 0.0 0.0 0.0 2.8565780708499918E-5 5.7131561416999836E-5 10-11 2.8565780708499918E-5 0.0 0.0 2.8565780708499918E-5 5.7131561416999836E-5 12-13 2.8565780708499918E-5 0.0 0.0 2.8565780708499918E-5 3.8563803956474893E-4 14-15 2.8565780708499918E-5 0.0 0.0 4.284867106274988E-5 4.427696009817487E-4 16-17 4.284867106274988E-5 0.0 0.0 5.7131561416999836E-5 4.8561827204449863E-4 18-19 5.7131561416999836E-5 0.0 0.0 7.14144517712498E-5 4.8561827204449863E-4 20-21 5.7131561416999836E-5 0.0 0.0 1.1426312283399967E-4 5.855985045242483E-4 22-23 5.7131561416999836E-5 0.0 0.0 1.8567757460524948E-4 5.998813948784983E-4 24-25 5.7131561416999836E-5 0.0 0.0 3.142235877934991E-4 6.284471755869983E-4 26-27 5.7131561416999836E-5 0.0 0.0 6.712958466497481E-4 6.570129562954982E-4 28-29 5.7131561416999836E-5 0.0 0.0 0.002456657140930993 6.570129562954982E-4 30-31 7.14144517712498E-5 0.0 0.0 0.008655431554675476 6.570129562954982E-4 32-33 8.569734212549975E-5 0.0 0.0 0.022395572075463936 6.85578737003998E-4 34-35 8.569734212549975E-5 0.0 0.0 0.044148414084986626 7.14144517712498E-4 36-37 8.569734212549975E-5 0.0 0.0 0.08042695558478152 7.14144517712498E-4 38-39 8.569734212549975E-5 0.0 0.0 0.13464480736951437 7.14144517712498E-4 40-41 1.428289035424996E-4 0.0 0.0 0.19290471712449997 7.14144517712498E-4 42-43 1.428289035424996E-4 0.0 0.0 0.2541925996345865 7.14144517712498E-4 44-45 1.428289035424996E-4 0.0 0.0 0.33320554907429734 7.712760791294979E-4 46-47 1.428289035424996E-4 0.0 0.0 0.42824390149147656 8.284076405464977E-4 48-49 1.428289035424996E-4 0.0 0.0 0.5279670419448498 8.855392019634975E-4 50-51 1.428289035424996E-4 0.0 0.0 0.6373454162776959 9.998023247974972E-4 52-53 1.428289035424996E-4 0.0 0.0 0.7411248975916762 0.0010997825572772468 54-55 1.428289035424996E-4 0.0 0.0 0.8572305132813741 0.0011140654476314968 56-57 1.428289035424996E-4 0.0 0.0 0.9970600098494812 0.0011140654476314968 58-59 1.428289035424996E-4 0.0 0.0 1.1485586278370103 0.0011140654476314968 60-61 1.428289035424996E-4 0.0 0.0 1.3130689589372615 0.0011140654476314968 62-63 1.713946842509995E-4 0.0 0.0 1.4772222177786563 0.0012283285704654966 64-65 1.713946842509995E-4 0.0 0.0 1.6511878222934209 0.0012283285704654966 66-67 1.713946842509995E-4 0.0 0.0 1.857818397048355 0.0012283285704654966 68-69 1.713946842509995E-4 0.0 0.0 2.0867017149752107 0.0012283285704654966 70-71 1.713946842509995E-4 0.0 0.0 2.328711009137622 0.0012283285704654966 72-73 1.713946842509995E-4 0.0 0.0 2.582132332693079 0.0012997430222367462 74-75 1.713946842509995E-4 0.0 0.0 2.838667326345763 0.001414006145070746 76-77 1.713946842509995E-4 0.0 0.0 3.1163552806130905 0.001456854816133496 78-79 1.713946842509995E-4 0.0 0.0 3.4349351499646357 0.001456854816133496 80-81 1.713946842509995E-4 0.0 0.0 3.7705687903991554 0.0014711377064877459 82-83 1.713946842509995E-4 0.0 0.0 4.121542255074139 0.0016139666100302454 84-85 1.713946842509995E-4 0.0 0.0 4.489512359270681 0.0016282495003844954 86-87 1.713946842509995E-4 0.0 0.0 4.862809981569359 0.001742512623218495 88-89 1.713946842509995E-4 0.0 0.0 5.269172495038124 0.0018567757460524947 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5245 0.0 51.505684 1 GTATCAA 9015 0.0 41.362106 1 ATCAACG 11635 0.0 31.788717 3 TATCAAC 11775 0.0 31.343906 2 TCAACGC 11840 0.0 31.19817 4 CAACGCA 12220 0.0 30.18911 5 AACGCAG 12775 0.0 28.924288 6 TAAGGTG 2215 0.0 28.76013 5 GTAAGGT 2095 0.0 28.365194 4 ACGCAGA 14635 0.0 25.150866 7 CGCAGAG 14810 0.0 24.789537 8 AGGTAAG 2420 0.0 22.797905 2 GGTAAGG 2600 0.0 22.490107 3 TGGTATC 2640 0.0 22.15917 2 GTACATG 10630 0.0 21.565826 1 GCAGAGT 17055 0.0 21.164394 9 TACATGG 10480 0.0 20.830692 2 GTGGTAT 3105 0.0 20.525583 1 AAGGTAA 2480 0.0 20.520332 1 AGAGTAC 14610 0.0 20.350172 10-11 >>END_MODULE