FastQCFastQC Report
Thu 26 May 2016
SRR938528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477929
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77580.22306378307320246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60730.17461541049285365No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA47950.13786940446455345No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA47110.13545417402137883No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA43400.12478690623069075No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42300.12162410445986677No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40530.11653486888317732No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA36870.10601136480934488No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC36380.10460248038415966No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.049.7266961
GTATCAA85200.043.4013331
TATCAAC112950.032.440942
ATCAACG112400.032.345993
TCAACGC114450.031.891194
CAACGCA116750.031.221775
AACGCAG122300.029.797636
ACGCAGA137700.026.3904387
CGCAGAG139850.025.9167888
TAAGGTG19200.024.7524285
TGGTATC24900.022.522052
GCAGAGT156250.022.3437069
GTACATG100100.022.2122651
TACATGG97200.022.1980512
GTAAGGT20000.021.861664
GAGTACT91900.021.57887612-13
AGAGTAC131700.021.29656410-11
ACATGGG100350.020.9802993
GTGGTAT28850.020.6730791
TAAGGTA12400.020.3132534
Produced by FastQC (version 0.11.5)