##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938528_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3477929 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.868242566193846 31.0 31.0 34.0 27.0 34.0 2 31.061097279444176 31.0 31.0 34.0 27.0 34.0 3 31.11077655696824 31.0 31.0 34.0 27.0 34.0 4 34.77749545778536 37.0 35.0 37.0 32.0 37.0 5 34.751514478875215 37.0 35.0 37.0 32.0 37.0 6 34.716918315468774 37.0 35.0 37.0 31.0 37.0 7 34.615166957117296 37.0 35.0 37.0 30.0 37.0 8 34.55898754689932 37.0 35.0 37.0 30.0 37.0 9 36.011484133229864 39.0 35.0 39.0 30.0 39.0 10-11 35.82701947049523 38.0 35.0 39.0 30.0 39.0 12-13 35.62450742956513 38.0 35.0 39.0 30.0 39.0 14-15 36.74850205395222 39.0 36.0 41.0 30.0 41.0 16-17 36.66394081075261 39.0 36.0 41.0 30.0 41.0 18-19 36.63434000521575 39.0 36.0 41.0 30.0 41.0 20-21 36.541287358080055 39.0 36.0 41.0 29.5 41.0 22-23 36.36876874139754 39.0 36.0 41.0 29.0 41.0 24-25 36.20322295250996 39.0 35.0 41.0 28.0 41.0 26-27 35.963696786219614 39.0 35.0 40.0 27.0 41.0 28-29 35.7019167153786 38.5 34.5 40.0 27.0 41.0 30-31 35.39974967861621 38.0 34.0 40.0 26.5 41.0 32-33 35.075272094398706 38.0 34.0 40.0 25.5 41.0 34-35 34.737761897957085 38.0 33.5 40.0 24.5 41.0 36-37 34.468793928800736 38.0 33.0 40.0 24.0 41.0 38-39 34.17539691005768 38.0 33.0 40.0 23.5 41.0 40-41 33.85772107481205 38.0 33.0 40.0 22.0 41.0 42-43 33.39577691206462 37.5 32.0 40.0 19.0 41.0 44-45 32.89304065724171 37.0 31.0 40.0 16.5 41.0 46-47 32.37447788612131 37.0 30.5 40.0 13.5 41.0 48-49 31.840156598941498 36.5 30.0 39.0 9.0 40.5 50-51 31.09396626555631 35.5 29.5 38.5 8.0 40.0 52-53 31.064286246211466 35.5 30.0 39.0 7.0 40.0 54-55 30.96591549166185 36.0 29.0 39.0 2.0 40.0 56-57 30.470604776578245 35.0 28.0 39.0 2.0 40.0 58-59 29.945999616438407 35.0 27.5 39.0 2.0 40.0 60-61 29.329313364361376 34.5 26.5 38.0 2.0 40.0 62-63 28.54050427711434 34.0 25.5 38.0 2.0 40.0 64-65 27.822203242216847 33.5 24.0 37.5 2.0 40.0 66-67 27.05118304600238 33.0 22.0 37.0 2.0 39.0 68-69 26.130407061213727 32.0 19.0 36.0 2.0 39.0 70-71 25.217935156238095 31.0 14.0 35.5 2.0 38.5 72-73 24.25919491168451 31.0 7.5 35.0 2.0 38.0 74-75 23.264633205565726 30.0 2.0 34.5 2.0 37.0 76-77 22.26816778030834 29.0 2.0 34.0 2.0 36.0 78-79 21.23716096562063 28.5 2.0 34.0 2.0 36.0 80-81 20.24039550548617 26.5 2.0 33.5 2.0 35.0 82-83 19.199831278901897 25.5 2.0 33.0 2.0 35.0 84-85 18.192942552881327 23.5 2.0 32.0 2.0 35.0 86-87 17.183943806788463 19.5 2.0 31.5 2.0 34.0 88-89 16.068459563148068 12.0 2.0 31.0 2.0 34.0 90-91 14.912940574692584 2.0 2.0 30.5 2.0 34.0 92-93 13.775369048649354 2.0 2.0 29.5 2.0 34.0 94-95 12.571951296303059 2.0 2.0 29.0 2.0 34.0 96-97 11.246859697250864 2.0 2.0 27.0 2.0 33.5 98-99 9.695504853606845 2.0 2.0 21.5 2.0 32.5 100-101 6.878867711215497 2.0 2.0 2.0 2.0 27.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6654628156099243 1101 2 0.6068525098460995 1101 3 0.5716993733633693 1101 4 0.3529839517301596 1101 5 0.4101238391191231 1101 6 0.5402245183542718 1101 7 0.5579000768769617 1101 8 0.5850889231949665 1101 9 0.6041167760087873 1101 10-11 0.7309220715204745 1101 12-13 0.7449113429239702 1101 14-15 0.8381318863490961 1101 16-17 0.8532626838660988 1101 18-19 0.7042839883104932 1101 20-21 0.8023833223539683 1101 22-23 0.8804976386590155 1101 24-25 0.9171119093049782 1101 26-27 1.0185490192395719 1101 28-29 1.0149522249377014 1101 30-31 1.0526401023674055 1101 32-33 1.1165193784048455 1101 34-35 1.1785136825671998 1101 36-37 1.3290880553160207 1101 38-39 1.4115880453282657 1101 40-41 1.4772123684454428 1101 42-43 1.615660278200707 1101 44-45 1.7082319657636802 1101 46-47 1.8414433965716483 1101 48-49 1.6044331904421334 1101 50-51 1.6223401682689058 1101 52-53 1.783214705810284 1101 54-55 2.1601976650382575 1101 56-57 2.31628526789385 1101 58-59 2.3594291412219555 1101 60-61 2.4443175695121617 1101 62-63 2.303194280776257 1101 64-65 2.409237157016147 1101 66-67 2.669784157946893 1101 68-69 2.794029905520471 1101 70-71 3.2688858497913564 1101 72-73 3.242795840309565 1101 74-75 3.2764352423870164 1101 76-77 3.5176418633139406 1101 78-79 3.7082610770808913 1101 80-81 3.9455932264950206 1101 82-83 4.143166863668995 1101 84-85 4.136518774534988 1101 86-87 4.176989506670196 1101 88-89 4.404030521032656 1101 90-91 4.49612380574473 1101 92-93 4.344800429931832 1101 94-95 4.09375520005023 1101 96-97 3.8947056195104395 1101 98-99 3.6964148683567988 1101 100-101 2.564056024723828 1102 1 0.7634343650434019 1102 2 0.6333296960096426 1102 3 0.6784766265558666 1102 4 0.6506928773775087 1102 5 0.6175119067658841 1102 6 0.6680930238935332 1102 7 0.6543887794243588 1102 8 0.7431257058952028 1102 9 0.7478690440466238 1102 10-11 0.8661549052856756 1102 12-13 0.937924361836032 1102 14-15 0.9452222599717786 1102 16-17 1.0330200056920447 1102 18-19 1.0706541980646307 1102 20-21 1.046203106691685 1102 22-23 1.0710137072384853 1102 24-25 1.1036052566439167 1102 26-27 0.9900680872522258 1102 28-29 1.0793505351183015 1102 30-31 0.9268437118090418 1102 32-33 1.0725796129911842 1102 34-35 1.1519480241839304 1102 36-37 1.3078548163030632 1102 38-39 1.3472770358602375 1102 40-41 1.381265933126123 1102 42-43 1.6862035182483268 1102 44-45 1.7360194123804362 1102 46-47 1.7568085656585524 1102 48-49 1.7139051443735944 1102 50-51 1.9419585268543074 1102 52-53 1.866652678161966 1102 54-55 1.9422764360541613 1102 56-57 2.179333598842934 1102 58-59 2.447395691600139 1102 60-61 2.367260093684571 1102 62-63 2.403460484688228 1102 64-65 2.6294385606504065 1102 66-67 2.7354107699407244 1102 68-69 2.692668342426284 1102 70-71 2.614000233874755 1102 72-73 2.825676175259389 1102 74-75 2.7050975330527507 1102 76-77 2.7826589448253465 1102 78-79 3.258459314834237 1102 80-81 3.6445794791866746 1102 82-83 3.921925536570704 1102 84-85 3.974853318738109 1102 86-87 3.9072228071638406 1102 88-89 4.0146307769273015 1102 90-91 3.8728566053956435 1102 92-93 3.6794413058218023 1102 94-95 3.71615347011843 1102 96-97 3.663656293998649 1102 98-99 3.4150470147378353 1102 100-101 2.567054380115237 1103 1 0.47697630116701717 1103 2 0.4487840219792574 1103 3 0.4022490345955987 1103 4 0.3724788488021389 1103 5 0.37876173431343574 1103 6 0.47346885166527386 1103 7 0.47356376692155777 1103 8 0.47203396334945324 1103 9 0.49356554714213985 1103 10-11 0.6034347859054634 1103 12-13 0.6962031570832394 1103 14-15 0.7166365424833714 1103 16-17 0.6871904717016619 1103 18-19 0.7444366837941914 1103 20-21 0.7314631110892194 1103 22-23 0.869010591238343 1103 24-25 0.896799474744526 1103 26-27 0.966486293056974 1103 28-29 1.0370956439357997 1103 30-31 0.9712339145700071 1103 32-33 1.1041508056722975 1103 34-35 1.0691536047450967 1103 36-37 1.218378672943345 1103 38-39 1.3962776469885938 1103 40-41 1.3753373413728056 1103 42-43 1.3854670654658108 1103 44-45 1.443110414458495 1103 46-47 1.6870739160819213 1103 48-49 1.6688461494695694 1103 50-51 1.9023006617293703 1103 52-53 2.1176328278075722 1103 54-55 2.103315494377579 1103 56-57 2.241876307769065 1103 58-59 2.432255848833652 1103 60-61 2.508329024005164 1103 62-63 2.8191026760414637 1103 64-65 2.976771125492526 1103 66-67 3.035592006683448 1103 68-69 3.2576105654676546 1103 70-71 3.269127676232525 1103 72-73 3.5108673334069067 1103 74-75 3.472165070819834 1103 76-77 3.512057748652065 1103 78-79 3.6824164086656133 1103 80-81 3.7325624268732227 1103 82-83 3.888971625564789 1103 84-85 4.040417852599145 1103 86-87 4.105375787573244 1103 88-89 4.370175850607609 1103 90-91 4.310481363962218 1103 92-93 4.277281797009481 1103 94-95 4.2351724150974785 1103 96-97 4.043406281064717 1103 98-99 3.519611804044059 1103 100-101 2.2203326741725258 1104 1 0.6320795968111739 1104 2 0.5839991905668036 1104 3 0.4889970499728413 1104 4 0.5480122905902292 1104 5 0.5309870818054918 1104 6 0.5659554431879243 1104 7 0.6121833655472528 1104 8 0.6251642891390929 1104 9 0.6817210546597252 1104 10-11 0.7139168950728987 1104 12-13 0.7482715024298869 1104 14-15 0.8501557364031811 1104 16-17 0.8677756689260576 1104 18-19 0.8548976521795524 1104 20-21 0.8431414574012095 1104 22-23 0.8836903173047546 1104 24-25 0.9535191742092053 1104 26-27 0.8129576036148052 1104 28-29 0.9842496744004237 1104 30-31 0.9708731203795935 1104 32-33 1.1118086082382845 1104 34-35 0.9655798192129765 1104 36-37 1.1000509138993664 1104 38-39 1.1804353562040077 1104 40-41 1.159409086564743 1104 42-43 1.2528408785573575 1104 44-45 1.3124327454156557 1104 46-47 1.4190366899664895 1104 48-49 1.4530443572916063 1104 50-51 1.5854380352953967 1104 52-53 1.6629515327385498 1104 54-55 1.9386662441494273 1104 56-57 2.153289935830376 1104 58-59 2.3121601600504214 1104 60-61 2.5507103064151906 1104 62-63 2.475625460583018 1104 64-65 2.436883945950484 1104 66-67 2.9012195402342478 1104 68-69 3.316521774437664 1104 70-71 3.2320242028020303 1104 72-73 3.4363122004122815 1104 74-75 3.3279392169678452 1104 76-77 3.609654678501091 1104 78-79 3.6529841099825298 1104 80-81 3.786319105641077 1104 82-83 3.74097927394315 1104 84-85 3.8590436966703408 1104 86-87 3.9218725256524536 1104 88-89 4.109222569443613 1104 90-91 3.942160418363052 1104 92-93 4.180376527325693 1104 94-95 4.181333338221185 1104 96-97 4.0734012302007585 1104 98-99 3.504742702417774 1104 100-101 2.3899615974113635 1105 1 0.5688591924546813 1105 2 0.4689458322279094 1105 3 0.2702081518431463 1105 4 0.3087097750814678 1105 5 0.3408040779650747 1105 6 0.4251444172855372 1105 7 0.48846650500278344 1105 8 0.4338627782832063 1105 9 0.4643234899982289 1105 10-11 0.56361097925069 1105 12-13 0.6211813260390358 1105 14-15 0.6541704061593876 1105 16-17 0.6798708474066828 1105 18-19 0.741776190168558 1105 20-21 0.6876717512361168 1105 22-23 0.5217599260015788 1105 24-25 0.6716113468752809 1105 26-27 0.5626449526440496 1105 28-29 0.6917927068688883 1105 30-31 0.8021357823774267 1105 32-33 0.7306850795872677 1105 34-35 0.8791806495175294 1105 36-37 1.0285323097413865 1105 38-39 1.1360534962475413 1105 40-41 1.159976728467889 1105 42-43 1.2701794068321064 1105 44-45 1.3999985128789092 1105 46-47 1.417551455334106 1105 48-49 1.3744093805168767 1105 50-51 1.2386646600950115 1105 52-53 1.4718523613817105 1105 54-55 1.5273311764542576 1105 56-57 1.7573674452555146 1105 58-59 1.9074747281360125 1105 60-61 2.0343894208091235 1105 62-63 2.237802193812822 1105 64-65 2.4200567025922197 1105 66-67 2.640205414176375 1105 68-69 2.674725559998354 1105 70-71 2.710864964018782 1105 72-73 2.9504085731398035 1105 74-75 3.065573785561625 1105 76-77 3.1638723800025623 1105 78-79 3.5134577709095822 1105 80-81 3.5202864795064848 1105 82-83 3.720573809856397 1105 84-85 3.514838554958583 1105 86-87 3.583418728445743 1105 88-89 3.6007526518583752 1105 90-91 3.478619235250733 1105 92-93 3.441517863807201 1105 94-95 3.439689808866527 1105 96-97 3.215690690434389 1105 98-99 2.971746786181848 1105 100-101 2.2602729322998902 1106 1 0.34609692751664767 1106 2 0.30190461109050304 1106 3 0.15273672859781584 1106 4 0.2054784912970149 1106 5 0.2770940448380088 1106 6 -0.027487584661201936 1106 7 0.27906286226986055 1106 8 -0.05878242090811625 1106 9 0.250416183897741 1106 10-11 0.26930074417835925 1106 12-13 0.5035118883124525 1106 14-15 0.5514809812090604 1106 16-17 0.147490197475868 1106 18-19 0.5211113159841005 1106 20-21 0.5520818634254496 1106 22-23 0.40335743204766317 1106 24-25 0.6004526683474722 1106 26-27 0.6545078850029071 1106 28-29 0.7122252738286434 1106 30-31 0.4501819252132009 1106 32-33 0.6365762954464174 1106 34-35 0.6145363484277198 1106 36-37 0.6395652524622193 1106 38-39 0.6798371411683135 1106 40-41 0.8129166235979497 1106 42-43 0.5009560557461796 1106 44-45 0.9388731766888228 1106 46-47 1.2372192247478182 1106 48-49 1.1218618227566033 1106 50-51 0.9779707219932092 1106 52-53 1.208073352080806 1106 54-55 1.0451186733885613 1106 56-57 1.618986936609847 1106 58-59 1.6095744390914781 1106 60-61 1.8725582887706587 1106 62-63 1.9165272261282134 1106 64-65 1.8683103400468823 1106 66-67 2.024280473265776 1106 68-69 2.321888630803901 1106 70-71 2.1649468529533493 1106 72-73 2.3332112287264835 1106 74-75 2.656239103056137 1106 76-77 2.6264558953109542 1106 78-79 2.7231157189325934 1106 80-81 2.6179373588071932 1106 82-83 2.9289891741536103 1106 84-85 2.865150490555518 1106 86-87 2.6593413555330088 1106 88-89 2.52042957450843 1106 90-91 2.5417029733303984 1106 92-93 2.7005930424946403 1106 94-95 2.6829215168253473 1106 96-97 2.6304249053275015 1106 98-99 2.4239025886160963 1106 100-101 1.7659300788565977 1107 1 0.3065349918864726 1107 2 0.11618149868339245 1107 3 0.10547422223542213 1107 4 0.20207022903886696 1107 5 0.13663032038098777 1107 6 0.13712290025800655 1107 7 0.21587325899263732 1107 8 0.21361870490333956 1107 9 0.3795521650105371 1107 10-11 0.33028636202256934 1107 12-13 0.26931964091584604 1107 14-15 0.45567142195911714 1107 16-17 0.40409311283128346 1107 18-19 0.5069707018214231 1107 20-21 0.6036760805834476 1107 22-23 0.49357706644808985 1107 24-25 0.4035721447453682 1107 26-27 0.4461747412273809 1107 28-29 0.5882183414875186 1107 30-31 0.599855923050626 1107 32-33 0.6749427060391184 1107 34-35 0.5877226059774898 1107 36-37 0.6091293494369623 1107 38-39 0.6631378435705955 1107 40-41 0.598127205102962 1107 42-43 0.8709891174553803 1107 44-45 0.8200146087388305 1107 46-47 0.7554613429919499 1107 48-49 0.782833260391552 1107 50-51 1.0499385610627172 1107 52-53 1.1086784514506114 1107 54-55 1.2650285430840817 1107 56-57 1.509496097109846 1107 58-59 1.4286643472470573 1107 60-61 1.4547535362955024 1107 62-63 1.615448458420012 1107 64-65 1.7961095183658244 1107 66-67 1.9436896944422664 1107 68-69 1.8572830962350686 1107 70-71 2.13122108740518 1107 72-73 2.1969031769044847 1107 74-75 2.2531708188329276 1107 76-77 2.376987653158011 1107 78-79 2.731916470337179 1107 80-81 2.773611690214949 1107 82-83 2.888488059900677 1107 84-85 2.865010886048413 1107 86-87 2.525183737456544 1107 88-89 2.9120703372972763 1107 90-91 2.9719415871582697 1107 92-93 2.979651117275621 1107 94-95 2.841307822891631 1107 96-97 2.5893566598126387 1107 98-99 2.402939049951433 1107 100-101 1.5536972618735971 1108 1 0.302190246346111 1108 2 0.27525025641377354 1108 3 0.20735851608189648 1108 4 0.2302898440431207 1108 5 0.1634546612109915 1108 6 0.2074070053779309 1108 7 0.14859674648906918 1108 8 0.22424761033452967 1108 9 0.3418541976956533 1108 10-11 0.2687642902957066 1108 12-13 0.22790868711408052 1108 14-15 0.32715248251606965 1108 16-17 0.4567188823369719 1108 18-19 0.39846987173635284 1108 20-21 0.3729555839278902 1108 22-23 0.41824974111373336 1108 24-25 0.554785028900973 1108 26-27 0.49925171517580225 1108 28-29 0.41620132725626036 1108 30-31 0.40969579245115995 1108 32-33 0.521180243655877 1108 34-35 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-0.43346414278881884 2316 1 -1.2255751667661023 2316 2 -1.0999807641358004 2316 3 -0.9592547179932183 2316 4 -0.8243874252584291 2316 5 -0.8422623388132777 2316 6 -0.6655619636445209 2316 7 -0.8238107063232789 2316 8 -0.7778902678716193 2316 9 -0.8402600179160586 2316 10-11 -1.0504662861313605 2316 12-13 -1.0587876174045832 2316 14-15 -1.2486082891424815 2316 16-17 -1.2959640944846313 2316 18-19 -1.2587295667043463 2316 20-21 -1.1390635532707662 2316 22-23 -1.2246751158571243 2316 24-25 -1.1419426055017468 2316 26-27 -1.2913243772154601 2316 28-29 -1.2935861948834315 2316 30-31 -1.172850236063752 2316 32-33 -1.2826266992199393 2316 34-35 -1.1748388450287592 2316 36-37 -1.3732217122568642 2316 38-39 -1.5485019490040273 2316 40-41 -1.4167100855194619 2316 42-43 -1.1971014454306257 2316 44-45 -1.273596076514231 2316 46-47 -0.9793331909234055 2316 48-49 -0.919196228214858 2316 50-51 -0.9394881135476254 2316 52-53 -0.9451097354720339 2316 54-55 -0.8415105652709052 2316 56-57 -0.8380609922396793 2316 58-59 -0.6409193382533154 2316 60-61 -0.7372497940396272 2316 62-63 -0.6213800931358229 2316 64-65 -0.5667186205446768 2316 66-67 -0.5079804069242293 2316 68-69 -0.3044787942843925 2316 70-71 -0.35077308494593495 2316 72-73 -0.5342140876639618 2316 74-75 -0.2712027140171145 2316 76-77 -0.20666743486168926 2316 78-79 -0.16796000591095606 2316 80-81 -0.18666310181595946 2316 82-83 -0.15889384116027472 2316 84-85 -0.008775837872242676 2316 86-87 0.0906936261028406 2316 88-89 -0.28581384531016774 2316 90-91 -0.2992205068249483 2316 92-93 -0.2344807015370538 2316 94-95 -0.19861050717401518 2316 96-97 -0.11230970382445449 2316 98-99 -0.12327136722073995 2316 100-101 0.09600288882282282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 14413.0 3 5911.0 4 6484.0 5 7447.0 6 9006.0 7 11503.0 8 14601.0 9 18876.0 10 23344.0 11 28885.0 12 34627.0 13 40147.0 14 45176.0 15 51250.0 16 57674.0 17 65002.0 18 66131.0 19 68679.0 20 73958.0 21 81119.0 22 88926.0 23 98847.0 24 107821.0 25 116993.0 26 128249.0 27 138842.0 28 151218.0 29 164457.0 30 178398.0 31 194985.0 32 214373.0 33 233384.0 34 252818.0 35 262078.0 36 238093.0 37 150343.0 38 33450.0 39 421.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.309753962295524 18.151266471138864 14.54546136796144 23.99351819860417 2 15.58990597973488 19.259773764635842 40.16129264202985 24.989027613599433 3 19.499990508224194 22.96742249965053 29.173174285686343 28.359412706438935 4 13.134635421011481 15.933535191634022 36.062428409436755 34.86940097791775 5 14.277308949061235 36.66824621232719 33.9858381791435 15.068606659468081 6 33.66623523194339 35.147967533937724 16.813267207499198 14.372530026619684 7 29.535815461772707 31.814297421081957 20.00317550443492 18.64671161271042 8 26.819663285097384 34.827363932081745 19.225643741239622 19.127329041581252 9 26.360897346474164 16.681963761560908 19.528345166889817 37.4287937250751 10-11 25.395753376074364 26.054731106308843 26.99503278637127 21.554482731245525 12-13 26.37003641906734 23.155135160398487 27.201412182591472 23.2734162379427 14-15 22.777688060750396 24.818869470545067 25.257856563228763 27.145585905475773 16-17 22.82409349991099 27.9134385559154 26.248951364297696 23.013516579875915 18-19 22.930476349482383 26.486275988447932 28.208243222119595 22.37500443995009 20-21 23.14480578986153 25.77229888819794 28.548064859242878 22.534830462697652 22-23 22.920548422743874 25.656478177507974 28.50474036840322 22.918233031344933 24-25 22.91044737479483 25.850402167290774 28.46503609754189 22.7741143603725 26-27 22.344141544536413 26.281576682613206 28.245914993200284 23.128366779650097 28-29 22.588257356283705 26.700450542761008 28.07966160900815 22.631630491947135 30-31 22.612773866652553 26.186530184439693 28.0230748078921 23.177621141015656 32-33 22.315052788646387 26.144286090648983 28.030098214378164 23.510562906326467 34-35 23.15427135309836 26.31085228030176 27.844202400950408 22.69067396564947 36-37 23.205389870522534 25.803481648346843 27.881244635785666 23.109883845344957 38-39 22.61851287144999 26.207760656999252 27.83139404138626 23.342332430164497 40-41 23.18153924371541 26.015090263794928 27.966219157929 22.83715133456066 42-43 22.845380966273165 26.02798283284018 27.75223306364844 23.37440313723821 44-45 22.778340917750413 26.34829368588292 27.968885917426906 22.904479478939763 46-47 23.356929212219548 26.528475311465456 27.211001050692197 22.9035944256228 48-49 22.996670289657622 26.594301747936168 27.136571325783194 23.27245663662302 50-51 23.17224928882764 26.784365063446568 27.136583195124704 22.90680245260109 52-53 23.447924548973305 26.377766694123395 27.225594365758965 22.948714391144335 54-55 22.923660453114692 26.647751543036147 27.43807187660714 22.99051612724202 56-57 22.98334798173409 26.838563600777714 27.155647680968613 23.022440736519577 58-59 22.93412026941232 26.321096833853204 27.198751391687598 23.54603150504688 60-61 22.532303583015246 26.578516688537146 27.533882944378156 23.355296784069456 62-63 22.75140772683622 26.56970726269578 27.670525709032358 23.00835930143564 64-65 22.558607271006682 26.64848203311334 27.3842638221115 23.40864687376848 66-67 22.42464916018699 26.649463252322303 27.353831361108377 23.572056226382326 68-69 23.033358852981664 26.264505763486774 27.500346851558316 23.201788531973243 70-71 22.819564996403273 26.345149937504768 27.057694749236187 23.77759031685577 72-73 22.778496196883836 26.422491396943187 27.110031421610024 23.688980984562953 74-75 22.78272457081664 26.725723366936442 27.01816000003451 23.47339206221241 76-77 22.948131619770855 26.305393293642865 27.063326954678967 23.683148131907313 78-79 22.967577863506808 26.55702571640867 26.957989132810216 23.5174072872743 80-81 23.226235659167514 26.435370082729783 26.66760411459122 23.670790143511486 82-83 23.02484712283229 26.483516009506925 26.810852158109427 23.680784709551354 84-85 22.938687120396082 26.540902187059146 26.775601715877272 23.744808976667496 86-87 23.265212996398198 26.506376586275305 26.3852238192062 23.8431865981203 88-89 23.084146766164146 26.566793420604995 26.240308327541072 24.108751485689783 90-91 22.943897373686475 26.54940188432394 26.29948838122947 24.207212360760114 92-93 23.18636370956054 26.023380293678528 26.54737078811943 24.2428852086415 94-95 22.851755471870018 25.82332387784838 26.6516554192554 24.673265231026203 96-97 23.17958509083202 25.520347585716614 26.521600589892625 24.77846673355874 98-99 22.912225641326476 25.740592646654125 26.10734837106569 25.23983334095371 100-101 23.824160183304183 24.62949866714955 26.37890896413359 25.167432185412675 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 269.0 1 230.5 2 258.0 3 339.0 4 311.0 5 290.0 6 473.5 7 1024.5 8 1669.0 9 1944.0 10 1739.0 11 1470.0 12 1476.0 13 1647.5 14 1855.0 15 2070.5 16 2373.5 17 2694.0 18 3019.0 19 3431.5 20 4169.0 21 5229.0 22 6411.5 23 8034.5 24 10085.5 25 12846.0 26 16481.5 27 20766.5 28 25175.5 29 29835.0 30 35427.0 31 43310.0 32 53049.5 33 57632.5 34 58505.5 35 62204.0 36 68556.0 37 75573.5 38 82003.0 39 87213.5 40 92400.5 41 99182.0 42 104739.0 43 109032.0 44 113677.0 45 120059.0 46 124913.0 47 128101.5 48 129951.5 49 129394.0 50 128851.5 51 128282.5 52 127753.0 53 128184.5 54 128363.5 55 122964.0 56 114603.5 57 106524.5 58 98962.5 59 88167.5 60 73540.0 61 61617.5 62 52233.5 63 41684.0 64 31520.5 65 24193.5 66 18458.5 67 14140.0 68 11026.5 69 8687.0 70 6816.5 71 5080.5 72 3675.0 73 2804.0 74 2150.0 75 1645.0 76 1220.5 77 841.0 78 552.0 79 349.0 80 233.0 81 143.5 82 85.5 83 61.0 84 32.5 85 18.0 86 16.0 87 10.0 88 7.5 89 5.5 90 4.5 91 5.0 92 3.0 93 1.0 94 0.5 95 1.5 96 1.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.387011925775368 2 0.029299045495178307 3 0.03550963806334172 4 0.04494053788907134 5 0.06457866161155101 6 0.05549279470627492 7 0.03783861027640299 8 0.03847117063056779 9 0.03548088531997059 10-11 0.03829865417034103 12-13 0.029945982221028664 14-15 0.027243224344142735 16-17 0.022772172749932502 18-19 0.028048301158534288 20-21 0.03539462708985721 22-23 0.03435952832849664 24-25 0.04356040620725725 26-27 0.037752352046289614 28-29 0.032274954434089946 30-31 0.035567143550083974 32-33 0.03004661682282761 34-35 0.03155613584981177 36-37 0.03407200089478537 38-39 0.015612739650521906 40-41 0.013082498233862738 42-43 0.01367192947297084 44-45 0.011616108321935267 46-47 0.02005503850136101 48-49 0.017237269650990573 50-51 0.011400462746651815 52-53 0.009646545401013075 54-55 0.00829516646257011 56-57 0.008697704869765887 58-59 0.003939125841844385 60-61 0.007403831418065176 62-63 0.012234292304414494 64-65 0.007691358851776446 66-67 0.0037666093816176235 68-69 0.006224968939848973 70-71 0.013326896552517316 72-73 0.011098558941254982 74-75 0.012176786817672241 76-77 0.005290504780287349 78-79 0.008769586728193704 80-81 0.011572979206878577 82-83 0.013341272924202882 84-85 0.008367048320997928 86-87 0.0025446177883447305 88-89 0.016906613102222617 90-91 0.026625040361663507 92-93 0.011069806197883855 94-95 0.021808955846999753 96-97 0.019724381952593052 98-99 0.011788624782162029 100-101 0.12531883198305657 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3477929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.28562346666451 #Duplication Level Percentage of deduplicated Percentage of total 1 75.64830854913032 41.06615593788147 2 13.354685894170398 14.499348999330202 3 4.680076887653718 7.6218267515462665 4 2.169313665776765 4.710501793657887 5 1.189912860490064 3.2297580751352655 6 0.6894325548378022 2.2455765646551282 7 0.4746111947380256 1.8035195227428567 8 0.3174645057874262 1.3787006900165564 9 0.23593106455533625 1.152689844108627 >10 1.122039635123245 10.740805258995294 >50 0.07250151994148435 2.707067821691434 >100 0.03990459419229111 4.101966081930781 >500 0.0027217500424583973 0.9923712759033901 >1k 0.0029885882655567714 3.349014031481349 >5k 1.0673529519845613E-4 0.4006973509236112 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7758 0.22306378307320246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6073 0.17461541049285365 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4795 0.13786940446455345 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4711 0.13545417402137883 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4340 0.12478690623069075 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4230 0.12162410445986677 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4053 0.11653486888317732 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3687 0.10601136480934488 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3638 0.10460248038415966 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 2.8752743371126897E-5 0.0 0.0 0.0 0.0 7 2.8752743371126897E-5 0.0 0.0 0.0 0.0 8 2.8752743371126897E-5 0.0 0.0 0.0 0.0 9 2.8752743371126897E-5 0.0 0.0 0.0 0.0 10-11 2.8752743371126897E-5 0.0 0.0 2.8752743371126897E-5 0.0 12-13 2.8752743371126897E-5 0.0 0.0 2.8752743371126897E-5 1.437637168556345E-4 14-15 2.8752743371126897E-5 0.0 0.0 2.8752743371126897E-5 1.437637168556345E-4 16-17 2.8752743371126897E-5 0.0 0.0 2.8752743371126897E-5 1.5814008854119795E-4 18-19 2.8752743371126897E-5 0.0 0.0 4.3129115056690344E-5 2.012692035978883E-4 20-21 2.8752743371126897E-5 0.0 0.0 8.625823011338069E-5 4.456675222524669E-4 22-23 5.7505486742253795E-5 1.4376371685563449E-5 0.0 1.2938734517007103E-4 4.6004389393803036E-4 24-25 1.0063460179894415E-4 2.8752743371126897E-5 0.0 2.5877469034014207E-4 5.175493806802841E-4 26-27 1.2938734517007103E-4 2.8752743371126897E-5 0.0 5.894312391081014E-4 5.319257523658476E-4 28-29 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.0020270684076644464 5.463021240514111E-4 30-31 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.008453306551111308 5.606784957369745E-4 32-33 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.02126265372294834 6.038076107936648E-4 34-35 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.04127456310925266 6.469367258503552E-4 36-37 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.07668356657079543 6.613130975359186E-4 38-39 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.13193196295841575 7.331949559637359E-4 40-41 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.1890780404085305 7.475713276492993E-4 42-43 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.2508245567980255 8.194531860771165E-4 44-45 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.3306709251396449 8.625823011338069E-4 46-47 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.4249942997686267 9.488405312471877E-4 48-49 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.5235155749297931 0.0010063460179894415 50-51 1.437637168556345E-4 2.8752743371126897E-5 0.0 0.6340411204484048 0.0010638515047316952 52-53 1.437637168556345E-4 5.7505486742253795E-5 0.0 0.738758611806049 0.001221991593272893 54-55 1.437637168556345E-4 5.7505486742253795E-5 0.0 0.8526194755557115 0.0012363679649584566 56-57 1.437637168556345E-4 5.7505486742253795E-5 0.0 0.9934647889591766 0.0012651207083295836 58-59 1.437637168556345E-4 5.7505486742253795E-5 0.0 1.1455236722773812 0.001279497080015147 60-61 1.437637168556345E-4 5.7505486742253795E-5 0.0 1.3133103062195923 0.0013226261950718373 62-63 1.437637168556345E-4 5.7505486742253795E-5 0.0 1.475303262372521 0.001380131681814091 64-65 1.437637168556345E-4 5.7505486742253795E-5 0.0 1.6449444482621698 0.001380131681814091 66-67 1.437637168556345E-4 5.7505486742253795E-5 0.0 1.845926124426347 0.001380131681814091 68-69 2.3002194696901518E-4 7.188185842781724E-5 0.0 2.068544239977297 0.001380131681814091 70-71 2.3002194696901518E-4 8.625823011338069E-5 0.0 2.3042736065054807 0.001380131681814091 72-73 2.3002194696901518E-4 8.625823011338069E-5 0.0 2.549750153036477 0.0014663899119274717 74-75 2.3002194696901518E-4 8.625823011338069E-5 0.0 2.7994533528430283 0.0015526481420408524 76-77 2.3002194696901518E-4 8.625823011338069E-5 0.0 3.0667819843360804 0.0015526481420408524 78-79 2.5877469034014207E-4 8.625823011338069E-5 0.0 3.3695483720340467 0.0015526481420408524 80-81 2.5877469034014207E-4 8.625823011338069E-5 0.0 3.6871511753115147 0.0015526481420408524 82-83 2.5877469034014207E-4 1.0063460179894415E-4 0.0 4.013149779653351 0.001595777257097543 84-85 2.5877469034014207E-4 1.1501097348450759E-4 0.0 4.335669302047282 0.001638906372154233 86-87 2.5877469034014207E-4 1.1501097348450759E-4 0.0 4.671141935329905 0.001638906372154233 88-89 2.5877469034014207E-4 1.2938734517007103E-4 0.0 5.020976563926405 0.00166765911552536 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4740 0.0 49.726696 1 GTATCAA 8520 0.0 43.401333 1 TATCAAC 11295 0.0 32.44094 2 ATCAACG 11240 0.0 32.34599 3 TCAACGC 11445 0.0 31.89119 4 CAACGCA 11675 0.0 31.22177 5 AACGCAG 12230 0.0 29.79763 6 ACGCAGA 13770 0.0 26.390438 7 CGCAGAG 13985 0.0 25.916788 8 TAAGGTG 1920 0.0 24.752428 5 TGGTATC 2490 0.0 22.52205 2 GCAGAGT 15625 0.0 22.343706 9 GTACATG 10010 0.0 22.212265 1 TACATGG 9720 0.0 22.198051 2 GTAAGGT 2000 0.0 21.86166 4 GAGTACT 9190 0.0 21.578876 12-13 AGAGTAC 13170 0.0 21.296564 10-11 ACATGGG 10035 0.0 20.980299 3 GTGGTAT 2885 0.0 20.673079 1 TAAGGTA 1240 0.0 20.313253 4 >>END_MODULE