FastQCFastQC Report
Thu 26 May 2016
SRR938528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3477929
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78290.22510522785255246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66910.19238460589621006No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA48220.13864572853557391No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA47210.1357417014550901No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA46810.134591591720245No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45440.13065246587840063No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC45100.1296748726037823No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA42290.12159535171649566No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG38740.11138812781974561No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA35230.10129591489648007No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50300.054.5023651
GTATCAA92000.043.639161
ATCAACG118950.033.501283
TATCAAC119950.033.3436552
TCAACGC120700.033.0549054
CAACGCA122650.032.529375
AACGCAG127650.031.3668336
TAAGGTG24100.029.3652135
GTAAGGT23150.029.1340794
ACGCAGA145300.027.3931717
CGCAGAG147400.026.9384568
AGGTAAG24100.025.4257662
GCAGAGT166300.023.5056089
AAGGTAA24300.023.4629421
GGTAAGG29000.022.9294683
GAGTACT103100.021.4679812-13
GTACATG101150.021.419361
AGAGTAC146950.021.02527210-11
TACATGG100200.020.953472
CAGAGTA156000.020.7037110-11
Produced by FastQC (version 0.11.5)