##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938528_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3477929 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.81092512239324 31.0 31.0 34.0 30.0 34.0 2 32.06546137083304 33.0 31.0 34.0 30.0 34.0 3 32.12774441341384 33.0 31.0 34.0 30.0 34.0 4 35.78094549946247 37.0 35.0 37.0 35.0 37.0 5 35.667598159709414 37.0 35.0 37.0 33.0 37.0 6 35.70010112339843 37.0 35.0 37.0 33.0 37.0 7 35.6201420443028 37.0 35.0 37.0 33.0 37.0 8 35.58382014123923 37.0 35.0 37.0 33.0 37.0 9 37.138213574802705 39.0 37.0 39.0 33.0 39.0 10-11 37.06398218595032 39.0 37.0 39.0 33.0 39.0 12-13 36.94322454541194 39.0 37.0 39.0 33.0 39.0 14-15 37.962179072660774 40.0 37.0 41.0 33.0 41.0 16-17 38.045098246686464 40.0 37.0 41.0 33.0 41.0 18-19 38.17370437981914 40.0 37.5 41.0 33.0 41.0 20-21 38.114994584420785 40.0 37.0 41.0 33.0 41.0 22-23 37.99021989810603 40.0 37.0 41.0 33.0 41.0 24-25 37.870105743964295 40.0 37.0 41.0 32.5 41.0 26-27 37.64711326769465 40.0 37.0 41.0 32.0 41.0 28-29 37.50713729348702 39.5 36.5 41.0 32.0 41.0 30-31 37.23913728543624 39.0 36.0 41.0 31.0 41.0 32-33 37.13990164836603 39.0 36.0 41.0 31.0 41.0 34-35 37.00874442807775 39.0 36.0 41.0 31.0 41.0 36-37 36.879002992873055 39.0 36.0 41.0 30.0 41.0 38-39 36.65558440094665 39.0 35.0 40.0 30.0 41.0 40-41 36.42797409032789 39.0 35.0 40.0 30.0 41.0 42-43 36.16034226115599 38.5 35.0 40.0 30.0 41.0 44-45 35.87067145419012 38.0 35.0 40.0 29.0 41.0 46-47 35.4721558433194 38.0 34.0 40.0 27.5 41.0 48-49 35.05392462008281 38.0 34.0 40.0 26.5 41.0 50-51 34.64286519362528 38.0 33.0 40.0 25.5 41.0 52-53 34.01755829403073 37.0 33.0 40.0 24.0 41.0 54-55 33.56248618071272 37.0 32.0 40.0 23.0 41.0 56-57 33.14843833787291 36.5 32.0 39.0 22.0 40.5 58-59 32.58743838646505 36.0 31.0 39.0 19.5 40.0 60-61 32.07139349308166 35.0 31.0 39.0 18.0 40.0 62-63 31.527856232832814 35.0 30.0 38.0 14.0 40.0 64-65 30.849908954438114 34.5 29.0 38.0 8.5 40.0 66-67 30.174882092187623 34.0 28.0 37.5 7.5 39.5 68-69 29.42623411806279 34.0 27.5 37.0 4.0 39.0 70-71 28.625342696760054 33.0 26.0 36.0 2.0 39.0 72-73 28.024862784720447 33.0 26.0 35.5 2.0 38.5 74-75 27.30016325807686 32.0 25.0 35.0 2.0 37.5 76-77 26.177653856648597 30.5 23.0 34.0 2.0 36.0 78-79 26.662560966598228 32.0 24.5 35.0 2.0 36.0 80-81 26.28728864217757 32.0 23.5 35.0 2.0 36.0 82-83 25.59121764705375 31.0 21.0 34.0 2.0 36.0 84-85 24.798170549197526 31.0 18.5 34.0 2.0 35.0 86-87 23.88680605613283 30.5 11.5 34.0 2.0 35.0 88-89 22.88990315788505 29.5 4.5 34.0 2.0 35.0 90-91 22.015153127047732 29.0 2.0 34.0 2.0 35.0 92-93 20.928487039269633 28.5 2.0 33.0 2.0 35.0 94-95 19.74673131625171 26.5 2.0 33.0 2.0 34.0 96-97 18.356752826179026 24.5 2.0 32.0 2.0 34.0 98-99 16.589121428298277 11.0 2.0 32.0 2.0 34.0 100-101 12.413801000537964 2.0 2.0 27.0 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3146544488614005 1101 2 0.2779745187632514 1101 3 0.2809323144376492 1101 4 0.1612228433852536 1101 5 0.15338128897577263 1101 6 0.15541691078510667 1101 7 0.23413828672361348 1101 8 0.2514890677979693 1101 9 0.3140253092741858 1101 10-11 0.27734822867623876 1101 12-13 0.2808712079102449 1101 14-15 0.3619840917185613 1101 16-17 0.4011112249213866 1101 18-19 0.3674940241334781 1101 20-21 0.3625705386482352 1101 22-23 0.25757401136903013 1101 24-25 0.31209094251688896 1101 26-27 0.31278583319591036 1101 28-29 0.45448314859058314 1101 30-31 0.5473513603050293 1101 32-33 0.46505441228303823 1101 34-35 0.3529191815108419 1101 36-37 0.46864158545437107 1101 38-39 0.6795291845332585 1101 40-41 0.7212543252944172 1101 42-43 0.7581581897477179 1101 44-45 0.7672758453470365 1101 46-47 0.9007131238250707 1101 48-49 0.8283308899332127 1101 50-51 1.1225535537589124 1101 52-53 1.1602071158774976 1101 54-55 1.0835281926389086 1101 56-57 1.290155573627132 1101 58-59 1.227268002347337 1101 60-61 1.1366254145577628 1101 62-63 1.323262458132561 1101 64-65 1.4873653243005158 1101 66-67 1.4863229695058813 1101 68-69 1.5897946888007262 1101 70-71 1.817721849898298 1101 72-73 2.0258928933875957 1101 74-75 1.9150388698122391 1101 76-77 1.99510588524603 1101 78-79 2.00761890251313 1101 80-81 2.145022623326703 1101 82-83 2.3277864977045652 1101 84-85 2.487089718962686 1101 86-87 2.482361441095314 1101 88-89 2.4328515876610908 1101 90-91 2.6332615098028143 1101 92-93 2.946794915153621 1101 94-95 2.6898681339147785 1101 96-97 2.71409352506916 1101 98-99 2.759775218566709 1101 100-101 2.5413542315344397 1102 1 0.24623599475598823 1102 2 0.2687174567307338 1102 3 0.25553075927597035 1102 4 0.2463199041581703 1102 5 0.13504148517417036 1102 6 0.2866426746452504 1102 7 0.30102534668678516 1102 8 0.31404661222901353 1102 9 0.3202401078274377 1102 10-11 0.37056060061645013 1102 12-13 0.40944519505328714 1102 14-15 0.5109347118488472 1102 16-17 0.38113117053346457 1102 18-19 0.3965214407746487 1102 20-21 0.33756767079874095 1102 22-23 0.33119747172747793 1102 24-25 0.3500685800387089 1102 26-27 0.46776746583826423 1102 28-29 0.3899568161089846 1102 30-31 0.3157258875784734 1102 32-33 0.27650417955611317 1102 34-35 0.403871823006412 1102 36-37 0.5824008338003921 1102 38-39 0.5271858328456958 1102 40-41 0.5926739651525068 1102 42-43 0.481864930206207 1102 44-45 0.6438796854912283 1102 46-47 0.5432884957832798 1102 48-49 0.5191838371077964 1102 50-51 0.7819704655985973 1102 52-53 0.7559280126409149 1102 54-55 0.6280206751244108 1102 56-57 0.8432039008273833 1102 58-59 1.0301295913084019 1102 60-61 0.9294935320938578 1102 62-63 1.0831389194237175 1102 64-65 1.1177527576464392 1102 66-67 1.237779949514156 1102 68-69 1.347152784608646 1102 70-71 1.6685558598941554 1102 72-73 1.7057656789579276 1102 74-75 1.5839692163084358 1102 76-77 1.6112904814078348 1102 78-79 1.7065140119487658 1102 80-81 1.9420786446110192 1102 82-83 1.9927586964341621 1102 84-85 2.1089691988060153 1102 86-87 2.0968589732452187 1102 88-89 1.8942485527640507 1102 90-91 2.093635046262367 1102 92-93 2.262152689687415 1102 94-95 2.2386113129315675 1102 96-97 2.0776661757568426 1102 98-99 2.0877542019159385 1102 100-101 1.608639744508368 1103 1 0.15212923375783305 1103 2 0.20711771239147936 1103 3 0.1729473246268256 1103 4 0.1620749979722973 1103 5 0.12888870524073326 1103 6 0.19742413212710375 1103 7 0.18498263452716657 1103 8 0.17088683072626765 1103 9 0.23372718251659563 1103 10-11 0.24727987066835766 1103 12-13 0.30033763107342537 1103 14-15 0.33118227272456124 1103 16-17 0.22509336787941692 1103 18-19 0.2640135618535169 1103 20-21 0.2664420844604223 1103 22-23 0.2921333549857934 1103 24-25 0.2249505578958093 1103 26-27 0.35396865640684183 1103 28-29 0.3191082315124376 1103 30-31 0.23633565248267274 1103 32-33 0.29538395002191464 1103 34-35 0.48179998470644847 1103 36-37 0.5703978740852804 1103 38-39 0.5558889257803159 1103 40-41 0.626382086153761 1103 42-43 0.5732042336276635 1103 44-45 0.6834669528959907 1103 46-47 0.6383282514612745 1103 48-49 0.5117931719587077 1103 50-51 0.5781486499330128 1103 52-53 0.7823128327299003 1103 54-55 0.8844527471836656 1103 56-57 1.002248034641866 1103 58-59 1.032532608363951 1103 60-61 1.0273695915985712 1103 62-63 1.1076880640595874 1103 64-65 1.2786282556028787 1103 66-67 1.259129023444519 1103 68-69 1.2506044742114888 1103 70-71 1.257689388745451 1103 72-73 1.373452105441448 1103 74-75 1.3859454405052425 1103 76-77 1.3015751842291543 1103 78-79 1.4490732278074923 1103 80-81 1.5030172763301977 1103 82-83 1.7133253751725022 1103 84-85 1.7007117445490287 1103 86-87 1.5857485896678654 1103 88-89 1.5729596755157012 1103 90-91 1.9893969735046788 1103 92-93 2.2712133142670616 1103 94-95 2.0038301065835924 1103 96-97 2.004122213316986 1103 98-99 1.9096536686067473 1103 100-101 1.7126513387018942 1104 1 0.20254796133236752 1104 2 0.2268056387889601 1104 3 0.21898991031257964 1104 4 0.22723776543821117 1104 5 0.1304126245074002 1104 6 0.11979314269520103 1104 7 0.1545111263157537 1104 8 0.1677613061865273 1104 9 0.2288553784361156 1104 10-11 0.25121944267194607 1104 12-13 0.27496614550557297 1104 14-15 0.35456053913458874 1104 16-17 0.3837084530754993 1104 18-19 0.3965893165348575 1104 20-21 0.3967508086498839 1104 22-23 0.3513774675963717 1104 24-25 0.3802080793658007 1104 26-27 0.4430059892450231 1104 28-29 0.38315193842376516 1104 30-31 0.5084692235097137 1104 32-33 0.5207882420082086 1104 34-35 0.5344752997359379 1104 36-37 0.5036503120886664 1104 38-39 0.5260545701755461 1104 40-41 0.5838537097479914 1104 42-43 0.5073096894522209 1104 44-45 0.5573002937432179 1104 46-47 0.8175479579304294 1104 48-49 0.7940013444756389 1104 50-51 0.9290423319211456 1104 52-53 0.9676688422264448 1104 54-55 1.0716187132753845 1104 56-57 0.9992171342050185 1104 58-59 1.1665438985604126 1104 60-61 1.0122661584119754 1104 62-63 1.0982658976694069 1104 64-65 1.3007702956781664 1104 66-67 1.1782414620948636 1104 68-69 1.40195247924758 1104 70-71 1.656529472992741 1104 72-73 1.7050543143729975 1104 74-75 1.7791563457866424 1104 76-77 1.7655269669447087 1104 78-79 1.9059606781043676 1104 80-81 1.9628785724231506 1104 82-83 2.0780127806916724 1104 84-85 2.3489842752101673 1104 86-87 2.2962062213755736 1104 88-89 2.2753975642800377 1104 90-91 2.4453786813793137 1104 92-93 2.527807901055194 1104 94-95 2.4611694092587157 1104 96-97 2.4285732088567897 1104 98-99 2.393843802178285 1104 100-101 2.1138961232327507 1105 1 0.08232702565653582 1105 2 0.15439293755297712 1105 3 0.09057033613033383 1105 4 0.1465882299924388 1105 5 0.005020283780226009 1105 6 0.022115653206753905 1105 7 0.06936565525115412 1105 8 0.09587006995856484 1105 9 0.16268535364230274 1105 10-11 0.22246255726525277 1105 12-13 0.21959467315161163 1105 14-15 0.2361025102674219 1105 16-17 0.1441750938114339 1105 18-19 0.21040993908736283 1105 20-21 0.20727731646802994 1105 22-23 0.3316684032103012 1105 24-25 0.2285568848094357 1105 26-27 0.27286265844286817 1105 28-29 0.30840347626911324 1105 30-31 0.29168942752200877 1105 32-33 0.33124251189693155 1105 34-35 0.32807080870304617 1105 36-37 0.2041858174789013 1105 38-39 0.16686759137587615 1105 40-41 0.3564605575453683 1105 42-43 0.35783108930764485 1105 44-45 0.3594622275631778 1105 46-47 0.36735845349077323 1105 48-49 0.27037624320409037 1105 50-51 0.417341986254101 1105 52-53 0.4106135827350954 1105 54-55 0.4294149286919833 1105 56-57 0.569431424317095 1105 58-59 0.6439133922303526 1105 60-61 0.7072395468383377 1105 62-63 0.8670783810351281 1105 64-65 0.9304319631869227 1105 66-67 0.9323661501361364 1105 68-69 0.9895512302073577 1105 70-71 1.1010978973784447 1105 72-73 1.308931723919219 1105 74-75 1.1267912417721675 1105 76-77 1.1502795760614468 1105 78-79 1.2188633414443437 1105 80-81 1.4875380931542175 1105 82-83 1.672405358248497 1105 84-85 1.7896883368549368 1105 86-87 1.9565670543122913 1105 88-89 1.9862592251432396 1105 90-91 2.0832504298173617 1105 92-93 2.2347830065779526 1105 94-95 2.156007035984569 1105 96-97 2.3175679435505785 1105 98-99 2.32447059122757 1105 100-101 2.152101395901207 1106 1 0.04524708983762693 1106 2 0.09393613046333371 1106 3 0.05728357161440556 1106 4 0.07121683025211212 1106 5 0.055970960593484165 1106 6 -0.04445779609308431 1106 7 0.01642375087408965 1106 8 -0.052477978185542895 1106 9 -0.1585891755161768 1106 10-11 0.026837485307098063 1106 12-13 0.07201396600510179 1106 14-15 0.1007363106496939 1106 16-17 -0.02996654311252911 1106 18-19 0.05400744162523097 1106 20-21 -0.004840005696863159 1106 22-23 0.13921849963821842 1106 24-25 0.038349843068445466 1106 26-27 -0.1229324898953692 1106 28-29 0.0661587311676044 1106 30-31 0.18909165453310806 1106 32-33 0.020620818981946343 1106 34-35 -0.048658977554524085 1106 36-37 -0.05202913431670453 1106 38-39 0.12668906977982175 1106 40-41 0.1690889859027962 1106 42-43 0.1831862237151114 1106 44-45 0.19613249227030138 1106 46-47 -0.21972530149207614 1106 48-49 0.026286259874460427 1106 50-51 0.2519896488460347 1106 52-53 0.16555353973720344 1106 54-55 0.12723936866221663 1106 56-57 0.22539045226253762 1106 58-59 0.5363194112965104 1106 60-61 0.5387940454307483 1106 62-63 0.5772694572356656 1106 64-65 0.8346110135657625 1106 66-67 0.7633323061245072 1106 68-69 0.7382584950385507 1106 70-71 0.59812843761393 1106 72-73 1.0113178323043819 1106 74-75 0.8956585495055656 1106 76-77 0.8801389080410331 1106 78-79 1.0608392939470228 1106 80-81 1.054750993285392 1106 82-83 1.2347573955452233 1106 84-85 1.4037796804128924 1106 86-87 1.4588998140002936 1106 88-89 1.4314667803584626 1106 90-91 1.5243826022311673 1106 92-93 1.5816649901065922 1106 94-95 1.4438394427786285 1106 96-97 1.6103570909377751 1106 98-99 1.878400813906353 1106 100-101 1.6277201004599338 1107 1 -7.289422930796263E-4 1107 2 0.08470503785238037 1107 3 0.07465305566529423 1107 4 0.08747919388711267 1107 5 0.06071535480360524 1107 6 -3.1794231226456304E-5 1107 7 -0.045018656310112704 1107 8 0.035898498890666986 1107 9 0.05526426434889942 1107 10-11 0.11786852994129049 1107 12-13 0.11698643411485676 1107 14-15 0.2666167686326659 1107 16-17 0.19959793631918643 1107 18-19 0.1473330738994747 1107 20-21 0.1608908622591727 1107 22-23 0.19539040294537813 1107 24-25 0.23152115086850245 1107 26-27 0.3021576053248438 1107 28-29 0.29601109429292194 1107 30-31 0.22866338291247246 1107 32-33 0.3174671142268579 1107 34-35 0.26190780749131193 1107 36-37 0.319957542301033 1107 38-39 0.2981296502693098 1107 40-41 0.4268843805193612 1107 42-43 0.416768429102369 1107 44-45 0.4534410253249561 1107 46-47 0.6581874919944894 1107 48-49 0.5117325767559535 1107 50-51 0.3859250794675262 1107 52-53 0.3869861819312064 1107 54-55 0.5889580084817396 1107 56-57 0.597901349394796 1107 58-59 0.8398101639726079 1107 60-61 0.7981164920542909 1107 62-63 0.8106923184009247 1107 64-65 1.0962035274022632 1107 66-67 1.005045963208186 1107 68-69 1.2010612384579566 1107 70-71 0.9842510247904102 1107 72-73 1.3281639286099782 1107 74-75 1.2534903469322956 1107 76-77 1.1913843228946597 1107 78-79 1.3818096027170306 1107 80-81 1.610316709996269 1107 82-83 1.7855805300901153 1107 84-85 1.765231363720936 1107 86-87 1.9471839847768955 1107 88-89 2.02671206135215 1107 90-91 2.1658172353604677 1107 92-93 2.128560885240592 1107 94-95 1.8179140183419875 1107 96-97 1.8747102347456845 1107 98-99 2.2432373956693716 1107 100-101 1.8836210222043785 1108 1 0.06520575593308564 1108 2 -0.004296586192275242 1108 3 0.030983967474085716 1108 4 0.04971159760576427 1108 5 -0.058165544557915894 1108 6 0.057708536046050085 1108 7 0.06737320211176012 1108 8 0.06684318479484119 1108 9 0.030813062236838107 1108 10-11 0.048826020000589665 1108 12-13 0.06638514865925771 1108 14-15 0.1938644768855582 1108 16-17 0.1760136002729027 1108 18-19 -0.035379670634029026 1108 20-21 -0.034812819292312724 1108 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-0.48402368253836414 2316 54-55 -0.4729369670837187 2316 56-57 -0.1809562736003869 2316 58-59 -0.2821717471674532 2316 60-61 -0.13282518310340308 2316 62-63 -0.16407538262856036 2316 64-65 -0.26465440875994517 2316 66-67 -0.09399711972061553 2316 68-69 0.15400818465281318 2316 70-71 -0.17483465147933686 2316 72-73 0.12078722609246739 2316 74-75 0.2213526567119537 2316 76-77 0.3312978905460682 2316 78-79 0.21710814300424985 2316 80-81 -0.35225775195484843 2316 82-83 0.1579898733779892 2316 84-85 0.40048600665546275 2316 86-87 0.492622922932096 2316 88-89 0.4406360186514533 2316 90-91 0.6505185448347639 2316 92-93 0.8364019413792434 2316 94-95 1.1336890669816988 2316 96-97 1.0849693283131217 2316 98-99 1.0631667573242645 2316 100-101 0.4796668837429827 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 15.0 4 43.0 5 120.0 6 258.0 7 670.0 8 1697.0 9 2975.0 10 4908.0 11 7579.0 12 11263.0 13 15577.0 14 21023.0 15 27728.0 16 34652.0 17 42874.0 18 43840.0 19 42987.0 20 45439.0 21 49043.0 22 53657.0 23 59398.0 24 66911.0 25 76278.0 26 87189.0 27 101513.0 28 118862.0 29 138663.0 30 160209.0 31 187324.0 32 218568.0 33 259757.0 34 313623.0 35 376657.0 36 428062.0 37 363707.0 38 112552.0 39 2300.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.182280535704066 19.013398928810442 15.162741953064494 23.641578582421 2 16.11615889016022 20.023683360713747 39.188945541210515 24.67121220791552 3 19.593785618085562 23.170862148879717 29.683789033971387 27.551563199063335 4 13.685011971204702 16.67512476534167 35.66162506480149 33.97823819865213 5 14.155624140649664 36.80900469763807 34.212825029227254 14.822546132485009 6 33.61166947341363 35.665592943386706 16.520895049898947 14.20184253330071 7 29.477139987619065 31.88506723397746 19.988044609306286 18.649748169097183 8 26.70253668276054 35.38112347351642 18.979001290423493 18.93733855329955 9 26.03654656707071 16.7396940526792 19.473455247232614 37.75030413301748 10-11 25.34583080908207 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22.604874254415634 36-37 23.20484443432336 25.81818333425343 27.98845161201281 22.9885206194104 38-39 22.59380584579935 26.19110027250989 27.987971779058363 23.227122102632404 40-41 23.115875639539162 26.003837810107477 28.113741925242934 22.766544625110424 42-43 22.870568601253655 26.046181823984938 27.86387821908597 23.219371355675438 44-45 22.795002329710123 26.342171088563344 28.040494545488002 22.822332036238528 46-47 23.360162050531528 26.558129805753573 27.30650653298552 22.77520161072938 48-49 22.93596275254613 26.65533137680499 27.2317088704226 23.176997000226287 50-51 23.144132614553087 26.768660314802283 27.255012968924895 22.83219410171973 52-53 23.4790158165966 26.412557588150882 27.270913236009132 22.83751335924339 54-55 22.987171388490104 26.679081142829542 27.40546169861432 22.928285770066037 56-57 22.994100799642545 26.830205561988187 27.195121004482843 22.980572633886432 58-59 22.930629118650785 26.43413652205091 27.179465135717262 23.455769223581044 60-61 22.576582213150413 26.70293154345589 27.457547293231116 23.26293895016258 62-63 22.727289717530176 26.704699837173212 27.607075935132663 22.960934510163952 64-65 22.500331735015102 26.779159939118895 27.37612482609969 23.344383499766312 66-67 22.410106290755405 26.80087167217811 27.308489315287645 23.48053272177884 68-69 23.06338261028082 26.402328535602294 27.400288795460703 23.134000058656188 70-71 22.827970730278093 26.548633762885004 26.997924195393896 23.625471311443004 72-73 22.789524493429628 26.62523388603398 27.017321403797812 23.567920216738585 74-75 22.78407638568815 26.944512093260098 26.871307608637206 23.400103912414544 76-77 22.83183469242759 26.5372151070364 27.23915583095572 23.39179436958029 78-79 22.894630683950133 26.777214255955194 27.158576267658134 23.169578792436532 80-81 23.120847379068664 26.713162794816682 26.933869170229137 23.23212065588552 82-83 22.973820216173955 26.836560693994798 26.99349476917565 23.196124320655592 84-85 22.84176550930918 26.953144993170874 26.961684997484003 23.243404500035943 86-87 23.141830304410487 26.93049982115151 26.622920670630084 23.30474920380792 88-89 22.900194339792446 27.11182430693669 26.354764573974915 23.633216779295953 90-91 22.76207314562533 27.01151044715695 26.655129958330985 23.571286448886735 92-93 22.93344635120572 26.638783110345393 26.82406648119161 23.60370405725728 94-95 22.678189812754866 26.451528963729114 26.91513839779581 23.955142825720202 96-97 22.90407925292311 26.202508188423078 26.87719140862154 24.01622115003227 98-99 22.60179836908689 26.530817621636327 26.508591751010442 24.358792258266345 100-101 23.041897796891973 26.08181165363568 26.77049765988509 24.10579288958726 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 289.0 1 220.0 2 237.0 3 315.0 4 271.0 5 271.5 6 500.5 7 1206.5 8 1915.5 9 2127.5 10 1876.0 11 1569.0 12 1604.0 13 1707.0 14 1887.5 15 2140.5 16 2438.5 17 2772.0 18 3103.5 19 3542.5 20 4131.5 21 5055.5 22 6276.5 23 7821.0 24 9837.5 25 12728.0 26 16580.0 27 21078.0 28 25518.5 29 30216.0 30 35625.0 31 43673.5 32 55237.5 33 59827.5 34 59299.5 35 62812.0 36 69097.5 37 76520.5 38 83751.0 39 88952.5 40 93829.0 41 101146.0 42 106543.5 43 111127.0 44 116660.0 45 124139.5 46 128483.0 47 130994.5 48 132566.0 49 131294.0 50 131209.0 51 130117.0 52 128916.0 53 129565.0 54 131023.0 55 124231.5 56 113943.5 57 104979.0 58 96182.0 59 84724.5 60 68647.0 61 57340.5 62 49212.0 63 37451.0 64 26409.0 65 19948.5 66 15075.0 67 11680.0 68 9183.5 69 7161.0 70 5692.5 71 4043.5 72 2834.0 73 2204.5 74 1677.5 75 1266.5 76 916.0 77 619.5 78 409.5 79 254.0 80 151.0 81 80.5 82 48.5 83 26.0 84 14.0 85 11.0 86 4.5 87 2.0 88 1.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024727359299169135 2 0.010983547967770475 3 0.001753917345638741 4 0.0 5 3.162801770823959E-4 6 0.0 7 0.0 8 2.8752743371126897E-5 9 2.87527433711269E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0017395409739531771 26-27 0.009143372392018354 28-29 2.0126920359788826E-4 30-31 0.0 32-33 4.1691477888134007E-4 34-35 0.010681644162373643 36-37 0.0011788624782162027 38-39 0.012665583454981397 40-41 0.012219915932728932 42-43 0.005046106461632771 44-45 8.194531860771165E-4 46-47 4.3129115056690344E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.188185842781724E-5 66-67 4.6004389393803036E-4 68-69 0.0010063460179894415 70-71 1.4376371685563449E-5 72-73 1.8689283191232483E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.437637168556345E-4 82-83 0.0010207223896750049 84-85 0.0051467410634317146 86-87 0.003594092921390862 88-89 0.0 90-91 0.0012938734517007103 92-93 3.7378566382464967E-4 94-95 4.3129115056690344E-5 96-97 1.4376371685563449E-5 98-99 0.0 100-101 4.3129115056690344E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3477929.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.549372072152075 #Duplication Level Percentage of deduplicated Percentage of total 1 72.29266135089334 36.543486367123954 2 15.107545294210947 15.273538563479185 3 5.279211257607598 8.005824423249008 4 2.527201074253636 5.109937096143579 5 1.3377086490855499 3.381016611338069 6 0.8139264180610178 2.4686081607552235 7 0.5271338776762102 1.8652400560143734 8 0.37828016192724506 1.5297459722219398 9 0.27577378890612786 1.2546172676846683 >10 1.3183842318272792 11.759410360298933 >50 0.08701873114293043 3.011724245513273 >100 0.04814993580879058 4.579660376602266 >500 0.0034741377672289314 1.1389095881726354 >1k 0.0034171846762071994 3.6602584854816684 >5k 1.1390615587357332E-4 0.4180224259211873 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7829 0.22510522785255246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6691 0.19238460589621006 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4822 0.13864572853557391 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4721 0.1357417014550901 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 4681 0.134591591720245 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4544 0.13065246587840063 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 4510 0.1296748726037823 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 4229 0.12159535171649566 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3874 0.11138812781974561 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 3523 0.10129591489648007 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.8752743371126897E-5 0.0 12-13 0.0 0.0 0.0 2.8752743371126897E-5 2.7315106202570555E-4 14-15 0.0 0.0 0.0 2.8752743371126897E-5 2.87527433711269E-4 16-17 0.0 0.0 0.0 2.8752743371126897E-5 3.4503292045352275E-4 18-19 0.0 0.0 0.0 4.3129115056690344E-5 3.7378566382464967E-4 20-21 0.0 0.0 0.0 8.625823011338069E-5 4.025384071957766E-4 22-23 0.0 0.0 0.0 1.2938734517007103E-4 4.3129115056690344E-4 24-25 1.4376371685563449E-5 0.0 0.0 2.5877469034014207E-4 4.3129115056690344E-4 26-27 2.8752743371126897E-5 0.0 0.0 6.038076107936648E-4 4.6004389393803036E-4 28-29 2.8752743371126897E-5 0.0 0.0 0.002012692035978883 4.6004389393803036E-4 30-31 5.7505486742253795E-5 0.0 0.0 0.00846768292279687 4.6004389393803036E-4 32-33 5.7505486742253795E-5 0.0 0.0 0.021406417439803975 4.6004389393803036E-4 34-35 8.625823011338069E-5 0.0 0.0 0.04144707956947942 5.175493806802841E-4 36-37 1.1501097348450759E-4 0.0 0.0 0.0772873741815891 5.175493806802841E-4 38-39 1.437637168556345E-4 0.0 0.0 0.1331827072950598 5.175493806802841E-4 40-41 1.437637168556345E-4 0.0 0.0 0.19067381766562802 5.175493806802841E-4 42-43 1.437637168556345E-4 0.0 0.0 0.2531535290110868 5.175493806802841E-4 44-45 1.437637168556345E-4 0.0 0.0 0.3336612104502421 5.319257523658476E-4 46-47 1.437637168556345E-4 0.0 0.0 0.428804038265301 5.463021240514111E-4 48-49 1.437637168556345E-4 0.0 0.0 0.5277134754619774 6.469367258503552E-4 50-51 1.437637168556345E-4 0.0 0.0 0.6385265484143006 6.900658409070455E-4 52-53 1.437637168556345E-4 0.0 0.0 0.7434596853472282 8.050768143915532E-4 54-55 1.437637168556345E-4 0.0 0.0 0.8577805929908286 8.3382955776268E-4 56-57 1.437637168556345E-4 0.0 0.0 0.9991578321466597 8.625823011338069E-4 58-59 1.437637168556345E-4 0.0 0.0 1.1520361686509415 9.200877878760607E-4 60-61 1.437637168556345E-4 0.0 0.0 1.3207860194960852 9.488405312471876E-4 62-63 1.437637168556345E-4 0.0 0.0 1.4837853216670036 0.0010063460179894415 64-65 1.437637168556345E-4 0.0 0.0 1.654418477202956 0.0010494751330461316 66-67 1.437637168556345E-4 0.0 0.0 1.8574272217747976 0.0010782278764172588 68-69 1.437637168556345E-4 0.0 0.0 2.0828056006893756 0.0010926042481028222 70-71 1.5814008854119795E-4 0.0 0.0 2.32313540615694 0.0011069806197883856 72-73 1.7251646022676138E-4 0.0 0.0 2.5751532018048673 0.0011788624782162029 74-75 1.7251646022676138E-4 0.0 0.0 2.833209073560731 0.0012938734517007103 76-77 1.7251646022676138E-4 0.0 0.0 3.1119237914287496 0.0013657553101285276 78-79 1.7251646022676138E-4 0.0 0.0 3.4295122183345317 0.001380131681814091 80-81 1.7251646022676138E-4 0.0 0.0 3.764711700555129 0.0013945080534996544 82-83 1.7251646022676138E-4 0.0 0.0 4.1134968540185834 0.001408884425185218 84-85 1.7251646022676138E-4 0.0 0.0 4.461376296065849 0.001437637168556345 86-87 1.7251646022676138E-4 0.0 0.0 4.82627736218882 0.0014663899119274717 88-89 1.7251646022676138E-4 0.0 0.0 5.214885065221285 0.0014951426552985987 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5030 0.0 54.502365 1 GTATCAA 9200 0.0 43.63916 1 ATCAACG 11895 0.0 33.50128 3 TATCAAC 11995 0.0 33.343655 2 TCAACGC 12070 0.0 33.054905 4 CAACGCA 12265 0.0 32.52937 5 AACGCAG 12765 0.0 31.366833 6 TAAGGTG 2410 0.0 29.365213 5 GTAAGGT 2315 0.0 29.134079 4 ACGCAGA 14530 0.0 27.393171 7 CGCAGAG 14740 0.0 26.938456 8 AGGTAAG 2410 0.0 25.425766 2 GCAGAGT 16630 0.0 23.505608 9 AAGGTAA 2430 0.0 23.462942 1 GGTAAGG 2900 0.0 22.929468 3 GAGTACT 10310 0.0 21.46798 12-13 GTACATG 10115 0.0 21.41936 1 AGAGTAC 14695 0.0 21.025272 10-11 TACATGG 10020 0.0 20.95347 2 CAGAGTA 15600 0.0 20.70371 10-11 >>END_MODULE