FastQCFastQC Report
Thu 26 May 2016
SRR938527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136129
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96940.45381154415299824No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74350.34805950389700246No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48890.22887194546771286No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51800.047.6554531
GTATCAA88600.038.813211
TCAACGC110350.030.77524
CAACGCA112100.030.2523375
TATCAAC114000.029.9150222
AACGCAG115500.029.3383966
ATCAACG117350.028.9394423
GTACATG91100.026.4706481
ACGCAGA130250.025.9077577
CGCAGAG129750.025.859868
TACATGG94850.024.9727882
ACATGGG94800.024.233373
GCAGAGT135850.024.031749
GAGTACT80300.023.01900512-13
TGTAGGA22800.022.1129172
GTCGGGA3450.022.0584962
AGTACTT85350.021.37864512-13
GTGTAGC14600.021.1755891
GTACTTT88250.020.75469614-15
AGAGTAC128300.020.6495910-11
Produced by FastQC (version 0.11.5)