##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938527_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2136129 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23260486609189 31.0 31.0 34.0 28.0 34.0 2 31.29935036694881 31.0 31.0 34.0 28.0 34.0 3 31.331899899303835 31.0 31.0 34.0 28.0 34.0 4 34.9979565840827 37.0 35.0 37.0 32.0 37.0 5 34.99483036839067 37.0 35.0 37.0 32.0 37.0 6 35.02101418032338 37.0 35.0 37.0 32.0 37.0 7 34.94747789108242 37.0 35.0 37.0 32.0 37.0 8 34.92793272316419 37.0 35.0 37.0 32.0 37.0 9 36.43388063174088 39.0 37.0 39.0 32.0 39.0 10-11 36.283617000658666 39.0 35.0 39.0 32.0 39.0 12-13 36.18760805176092 39.0 35.0 39.0 32.0 39.0 14-15 37.16291548871814 39.0 36.0 41.0 31.5 41.0 16-17 37.01438209022021 39.0 36.0 41.0 31.0 41.0 18-19 37.21840698759298 39.5 36.5 41.0 31.0 41.0 20-21 37.24381720392355 40.0 37.0 41.0 31.0 41.0 22-23 37.15142765254346 39.0 36.0 41.0 31.0 41.0 24-25 37.05329570451972 39.0 36.0 41.0 31.0 41.0 26-27 36.88765566124518 39.0 36.0 41.0 30.5 41.0 28-29 36.698285543616514 39.0 36.0 41.0 30.0 41.0 30-31 36.471123700862634 39.0 35.5 40.0 30.0 41.0 32-33 36.22514557875484 39.0 35.0 40.0 30.0 41.0 34-35 36.00532481886628 38.0 35.0 40.0 29.0 41.0 36-37 35.7687550705037 38.0 35.0 40.0 28.5 41.0 38-39 35.637388706393665 38.0 35.0 40.0 27.0 41.0 40-41 35.55702956141694 38.0 35.0 40.0 27.0 41.0 42-43 35.29452949704817 38.0 34.5 40.0 26.5 41.0 44-45 34.93179695608271 38.0 34.0 40.0 24.5 41.0 46-47 34.511086175039054 38.0 33.5 40.0 24.0 41.0 48-49 34.155297269031976 38.0 33.0 40.0 23.0 41.0 50-51 33.49517913009935 37.5 32.5 39.5 22.0 40.5 52-53 33.56195388948888 38.0 33.0 39.5 21.5 40.5 54-55 33.73384004430444 38.0 33.0 40.0 20.5 41.0 56-57 33.48992289323351 38.0 33.0 40.0 18.5 41.0 58-59 33.18851459813523 38.0 32.5 40.0 16.5 41.0 60-61 32.81315805365687 37.0 31.5 40.0 13.5 41.0 62-63 32.31716787703364 37.0 31.0 39.5 8.5 41.0 64-65 31.884766556701397 36.0 31.0 39.0 8.0 40.0 66-67 31.40093318334239 36.0 30.0 39.0 4.5 40.0 68-69 30.85439924274236 35.0 29.0 38.5 2.0 40.0 70-71 30.278504715773252 35.0 28.5 38.0 2.0 40.0 72-73 29.66257164244294 34.0 28.0 37.5 2.0 39.5 74-75 29.00765075517443 34.0 27.5 37.0 2.0 39.0 76-77 28.263437741821775 34.0 26.0 36.0 2.0 39.0 78-79 27.496723278416237 33.0 25.5 35.5 2.0 38.0 80-81 26.78392222567083 32.5 24.5 35.0 2.0 37.0 82-83 26.043130588087145 32.0 21.5 35.0 2.0 36.5 84-85 25.314758846492886 31.0 19.5 35.0 2.0 36.0 86-87 24.5423827399937 31.0 15.5 34.0 2.0 35.5 88-89 23.713364455049298 30.5 7.5 34.0 2.0 35.0 90-91 22.78353835372302 29.5 2.0 34.0 2.0 35.0 92-93 21.834780811458483 29.0 2.0 34.0 2.0 35.0 94-95 20.80153001059393 29.0 2.0 34.0 2.0 35.0 96-97 19.493593083563773 26.5 2.0 33.5 2.0 35.0 98-99 17.940982964980112 24.5 2.0 33.0 2.0 34.0 100-101 14.23055021489807 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.4921052784727493 1101 2 0.45887509327595666 1101 3 0.38572956088331267 1101 4 0.27022541655023957 1101 5 0.2348172961728281 1101 6 0.3620631592748893 1101 7 0.3961210280680092 1101 8 0.3679095576379723 1101 9 0.5509983871802433 1101 10-11 0.5929893212802142 1101 12-13 0.5971441440248029 1101 14-15 0.7238442574001382 1101 16-17 0.7526254313307916 1101 18-19 0.832292197942401 1101 20-21 0.8514379597301911 1101 22-23 0.7682809809809683 1101 24-25 0.6069997330339234 1101 26-27 0.5900454973670719 1101 28-29 0.7142822071378987 1101 30-31 0.7744653093999716 1101 32-33 0.7772797706005008 1101 34-35 0.7005101791929675 1101 36-37 0.7191667457502504 1101 38-39 0.7954341901479367 1101 40-41 0.7704439295557322 1101 42-43 0.850993162786942 1101 44-45 0.9324793772401421 1101 46-47 0.9592345719593709 1101 48-49 0.8929818477639273 1101 50-51 1.0248035800855249 1101 52-53 1.0277053843530979 1101 54-55 0.9412634120420478 1101 56-57 0.9172582645598908 1101 58-59 0.8870065184230995 1101 60-61 1.0339101884407071 1101 62-63 0.7960149407289805 1101 64-65 0.8126199588551799 1101 66-67 0.7525978941399778 1101 68-69 0.8668795230183584 1101 70-71 1.0760981837526913 1101 72-73 1.2000413441295095 1101 74-75 1.1929688974068107 1101 76-77 1.3506808067152143 1101 78-79 1.4319518613893685 1101 80-81 1.4712099623070038 1101 82-83 1.554848510950091 1101 84-85 1.4593681607579505 1101 86-87 1.430658969327208 1101 88-89 1.5504071115394318 1101 90-91 1.5296017763457037 1101 92-93 1.4590756039726074 1101 94-95 1.6631294487227635 1101 96-97 1.5090990268380864 1101 98-99 1.5097846653442843 1101 100-101 1.4044311292812264 1102 1 0.5138421694867645 1102 2 0.5111887088515772 1102 3 0.5004177630641884 1102 4 0.4326914275082885 1102 5 0.40891845099545776 1102 6 0.3705011334314108 1102 7 0.44113893736903975 1102 8 0.5036956461135702 1102 9 0.649019350398035 1102 10-11 0.7852691141375203 1102 12-13 0.719775909909778 1102 14-15 1.0430678908099438 1102 16-17 0.9714451128907555 1102 18-19 0.8784896638138591 1102 20-21 0.9061190046289909 1102 22-23 0.921171578651574 1102 24-25 0.9006655920472681 1102 26-27 0.7827445867719334 1102 28-29 0.7796651056332564 1102 30-31 0.8444003787944894 1102 32-33 0.8738022114161055 1102 34-35 0.7376548099437414 1102 36-37 0.9651755147621159 1102 38-39 1.0334883966339135 1102 40-41 1.1865543062197759 1102 42-43 1.1520239401729455 1102 44-45 1.2177678897487567 1102 46-47 1.280499962261949 1102 48-49 1.275199220966293 1102 50-51 1.1872363263142702 1102 52-53 1.1424676273830627 1102 54-55 1.0914244240624171 1102 56-57 1.1126086065427714 1102 58-59 1.1383687627407326 1102 60-61 1.1921263619667073 1102 62-63 1.3088846352729178 1102 64-65 1.3897005067145471 1102 66-67 1.0533276793786008 1102 68-69 1.1034175016817152 1102 70-71 1.2314543953507062 1102 72-73 1.3137369697102983 1102 74-75 1.4283600477011476 1102 76-77 1.5587667112690475 1102 78-79 1.5776832991338914 1102 80-81 1.6384333660828716 1102 82-83 1.7255837580426494 1102 84-85 1.7664769943677712 1102 86-87 1.8687932794274538 1102 88-89 1.8705970316826175 1102 90-91 1.9389403662216367 1102 92-93 2.195931207147993 1102 94-95 2.3658173461558007 1102 96-97 2.3141650304993533 1102 98-99 2.695725649422279 1102 100-101 2.396016494088002 1103 1 0.48991621698187515 1103 2 0.4132855083138587 1103 3 0.4435833288563913 1103 4 0.3950223165128506 1103 5 0.2951252604136201 1103 6 0.4584158565113725 1103 7 0.3357824713529567 1103 8 0.48910674313034264 1103 9 0.5113641372057387 1103 10-11 0.6142697862858171 1103 12-13 0.6250733734343683 1103 14-15 0.8048786229462124 1103 16-17 0.8370538619368588 1103 18-19 0.7735631452893514 1103 20-21 0.7547844364969123 1103 22-23 0.8531852945198324 1103 24-25 0.8140971870602343 1103 26-27 0.7565688326265914 1103 28-29 0.8360861433695845 1103 30-31 0.8802795790896312 1103 32-33 1.0716116649544887 1103 34-35 1.0045058962465703 1103 36-37 1.0537209454171261 1103 38-39 0.9865011876665477 1103 40-41 1.0970712792277126 1103 42-43 1.045923309970739 1103 44-45 1.0668908120271823 1103 46-47 0.6276903318424374 1103 48-49 0.7169119609560894 1103 50-51 0.9803799083087767 1103 52-53 1.1201558415406296 1103 54-55 0.9949005173141501 1103 56-57 1.0150488080861848 1103 58-59 0.9749343881671493 1103 60-61 1.098307346739766 1103 62-63 1.092253765977965 1103 64-65 1.2284345684612177 1103 66-67 1.1790178948198111 1103 68-69 1.3942393924904728 1103 70-71 1.4311323453489386 1103 72-73 1.4385283763195922 1103 74-75 1.565899656440088 1103 76-77 1.550422470265982 1103 78-79 1.5196451888308182 1103 80-81 1.5177054244233226 1103 82-83 1.5506896340338123 1103 84-85 1.7545702410461956 1103 86-87 1.6438630211304641 1103 88-89 1.6539660360440003 1103 90-91 1.5778580053133773 1103 92-93 1.6646179405578074 1103 94-95 1.5766887131437173 1103 96-97 1.7919060563088358 1103 98-99 1.9523931836487805 1103 100-101 1.634692185061505 1104 1 0.5081861912163461 1104 2 0.3936775345599628 1104 3 0.3920237172188408 1104 4 0.46970571927592175 1104 5 0.34806664680380806 1104 6 0.4180175018891603 1104 7 0.4339784083451761 1104 8 0.4135861390321267 1104 9 0.5340344740133318 1104 10-11 0.5639781100036743 1104 12-13 0.6053414491560005 1104 14-15 0.6464784173929488 1104 16-17 0.7940213802380001 1104 18-19 0.8102373933188645 1104 20-21 0.7296664931715569 1104 22-23 0.7578223764247198 1104 24-25 0.721876926264521 1104 26-27 0.7271330811081427 1104 28-29 0.8605637549547893 1104 30-31 0.8527161704264117 1104 32-33 0.7466003048056535 1104 34-35 0.6507919007811154 1104 36-37 0.7981152175230264 1104 38-39 0.8058516250620187 1104 40-41 0.6885506917831137 1104 42-43 0.6737305752319998 1104 44-45 0.6286620674009527 1104 46-47 0.6274517092396721 1104 48-49 0.35707787860631157 1104 50-51 0.5489426294153432 1104 52-53 0.6917419086452057 1104 54-55 0.6373705824298099 1104 56-57 0.7066376587246168 1104 58-59 0.7110280195844396 1104 60-61 0.7535815486149673 1104 62-63 0.9254848777975155 1104 64-65 0.9165131925866064 1104 66-67 0.9616325847412774 1104 68-69 1.2101302779635112 1104 70-71 1.2257283641818049 1104 72-73 1.180081897489913 1104 74-75 1.229953885389314 1104 76-77 1.1452417665791081 1104 78-79 1.3145799899033115 1104 80-81 1.5357690127077355 1104 82-83 1.4756474646855189 1104 84-85 1.3821826589378965 1104 86-87 1.425176587408309 1104 88-89 1.5873370382827225 1104 90-91 1.8114294457802522 1104 92-93 1.5712931878046064 1104 94-95 1.6780765545726943 1104 96-97 1.9089009579216203 1104 98-99 2.2375975116870563 1104 100-101 2.0323186871586714 1105 1 0.44019270484005446 1105 2 0.26876212563583834 1105 3 0.30616920766082245 1105 4 0.40173928430528605 1105 5 0.3947334258151116 1105 6 0.48476839746807343 1105 7 0.47043217305825635 1105 8 0.411265483199621 1105 9 0.6009765032944827 1105 10-11 0.5777488276109395 1105 12-13 0.5330345939750387 1105 14-15 0.6837521501232828 1105 16-17 0.6770229421240828 1105 18-19 0.6065416391478564 1105 20-21 0.6514260673735706 1105 22-23 0.8002460759394836 1105 24-25 0.8438355278576282 1105 26-27 0.8866893671009208 1105 28-29 0.7728613239647046 1105 30-31 0.8109638779306465 1105 32-33 0.976530600872195 1105 34-35 0.9092558864908753 1105 36-37 0.8478184059524736 1105 38-39 0.7122491051127184 1105 40-41 0.7123787672396915 1105 42-43 0.8426202613736038 1105 44-45 0.9867007251900262 1105 46-47 0.9078288856582972 1105 48-49 0.8875237922032326 1105 50-51 0.9233103582414017 1105 52-53 0.8693206393223036 1105 54-55 0.8634025935554774 1105 56-57 0.8995303675282074 1105 58-59 1.0279416670629402 1105 60-61 1.115753971613806 1105 62-63 1.0883851166481264 1105 64-65 1.2342410430287032 1105 66-67 1.4330017761171128 1105 68-69 1.202098006080032 1105 70-71 1.0996774614187501 1105 72-73 1.0995292417070743 1105 74-75 1.0596353528704228 1105 76-77 1.0394980436138859 1105 78-79 1.1541378812418053 1105 80-81 1.1697331168228295 1105 82-83 1.1865765873419143 1105 84-85 1.1451438299710723 1105 86-87 1.2962848435860082 1105 88-89 1.2293673394047815 1105 90-91 1.4062271057006228 1105 92-93 1.476761829016933 1105 94-95 1.4331646578966435 1105 96-97 1.8498013972187088 1105 98-99 2.247412959015307 1105 100-101 2.0313481625476584 1106 1 0.21359310749705784 1106 2 0.13643286636717278 1106 3 0.02397677170649004 1106 4 -0.06850933939035286 1106 5 0.1054096907043558 1106 6 -0.011834372402304894 1106 7 0.2023114241696078 1106 8 0.2157151142695568 1106 9 0.14622806491404816 1106 10-11 0.08663341089280152 1106 12-13 0.1596682626262904 1106 14-15 0.28612663835415475 1106 16-17 0.13816927484170805 1106 18-19 0.08844155258133668 1106 20-21 0.20009756615384333 1106 22-23 0.2016295475453589 1106 24-25 0.013909899303335749 1106 26-27 0.24102279413214944 1106 28-29 0.32357839263550403 1106 30-31 0.07641316075436322 1106 32-33 0.31109104655848796 1106 34-35 -0.12291039871201548 1106 36-37 0.10023170060708964 1106 38-39 0.11309535634330814 1106 40-41 0.3020614241043731 1106 42-43 0.034763176629247994 1106 44-45 0.23084553965743737 1106 46-47 0.227437343263297 1106 48-49 0.39412335941367616 1106 50-51 0.14274777056105847 1106 52-53 0.032997869744640695 1106 54-55 0.2856305816178164 1106 56-57 -0.6729902937177599 1106 58-59 -0.21194692427894068 1106 60-61 0.36524031919969246 1106 62-63 0.3689438301430741 1106 64-65 0.3145865902337164 1106 66-67 -0.11353816738004596 1106 68-69 -0.18533850570674204 1106 70-71 -0.17500508137270288 1106 72-73 0.13878205770178198 1106 74-75 0.421971555540253 1106 76-77 0.5054311476241402 1106 78-79 0.6309898387170279 1106 80-81 0.7939014045491106 1106 82-83 0.38058373908815923 1106 84-85 0.5512967466515484 1106 86-87 0.35880343429511186 1106 88-89 0.39023115791383134 1106 90-91 0.672647087199234 1106 92-93 0.5567164063454619 1106 94-95 0.26359080968120097 1106 96-97 0.5656126796214274 1106 98-99 1.0058766551000957 1106 100-101 0.062622308946235 1107 1 0.1887558446300055 1107 2 0.12157449696831435 1107 3 0.16506527782295422 1107 4 0.11344340688993526 1107 5 0.20883314492142802 1107 6 0.22931618497276673 1107 7 0.2895196161771807 1107 8 0.1850002585278716 1107 9 0.4035599518330244 1107 10-11 0.4031895929382401 1107 12-13 0.3947573907036812 1107 14-15 0.5372514338012166 1107 16-17 0.5042349746894885 1107 18-19 0.5036940995485892 1107 20-21 0.5943145502392113 1107 22-23 0.5191687423604492 1107 24-25 0.46867341448747624 1107 26-27 0.47449794807575785 1107 28-29 0.5396734007886508 1107 30-31 0.6829345225053345 1107 32-33 0.5775182327480621 1107 34-35 0.35998288243397525 1107 36-37 0.5043513331163254 1107 38-39 0.5004141812550955 1107 40-41 0.48318840655311845 1107 42-43 0.7444359105530793 1107 44-45 0.5752170932417755 1107 46-47 0.49516835074690135 1107 48-49 0.6102595419598273 1107 50-51 0.5072219456300289 1107 52-53 0.6572576358865021 1107 54-55 0.6101101445319799 1107 56-57 0.5931781558966804 1107 58-59 0.5126285604874639 1107 60-61 0.39152727244150753 1107 62-63 0.5182309027699432 1107 64-65 0.5767815040168074 1107 66-67 0.5681643254915087 1107 68-69 0.6514017155301026 1107 70-71 0.9863611925785314 1107 72-73 0.9142050211366417 1107 74-75 0.947689412460683 1107 76-77 1.0594128459168068 1107 78-79 1.1187487330583608 1107 80-81 1.2818213755987564 1107 82-83 1.0085433037651619 1107 84-85 1.307743694480699 1107 86-87 1.4125702601901189 1107 88-89 1.3525474844296639 1107 90-91 1.2704683410898525 1107 92-93 1.3794861974174886 1107 94-95 1.516826982482062 1107 96-97 1.522246422636524 1107 98-99 1.474021434197411 1107 100-101 1.1720736736083843 1108 1 0.3726674647100836 1108 2 0.1765992043464486 1108 3 0.2960368661025541 1108 4 0.21353989724241274 1108 5 0.13199274520736992 1108 6 0.31175504164779255 1108 7 0.22634475085533268 1108 8 0.32112848482950795 1108 9 0.26198954866064383 1108 10-11 0.343124802237611 1108 12-13 0.3126844310915047 1108 14-15 0.44178043081576845 1108 16-17 0.5657147555747102 1108 18-19 0.3667570481606717 1108 20-21 0.3550511436760573 1108 22-23 0.3652855854219652 1108 24-25 0.5374143716677438 1108 26-27 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2316 72-73 -2.138990354802182 2316 74-75 -1.9876800522908447 2316 76-77 -2.266199420076113 2316 78-79 -2.46296889021356 2316 80-81 -2.7371136715140203 2316 82-83 -2.96229677414723 2316 84-85 -2.944575806885762 2316 86-87 -3.1679818673779963 2316 88-89 -3.3879313267629136 2316 90-91 -3.233311567036683 2316 92-93 -3.368653902862185 2316 94-95 -3.89349085819045 2316 96-97 -4.123271659263768 2316 98-99 -3.9442785155347853 2316 100-101 -3.5934837069361905 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7702.0 3 1843.0 4 1809.0 5 1904.0 6 2260.0 7 2613.0 8 3405.0 9 4395.0 10 6027.0 11 7971.0 12 10870.0 13 13937.0 14 16730.0 15 20447.0 16 25321.0 17 30164.0 18 27018.0 19 22480.0 20 23514.0 21 25446.0 22 28216.0 23 31787.0 24 36006.0 25 40829.0 26 46479.0 27 53270.0 28 61464.0 29 71075.0 30 83013.0 31 96949.0 32 115544.0 33 137491.0 34 167230.0 35 207803.0 36 260211.0 37 288866.0 38 147327.0 39 6713.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 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28.814037529379092 31.205855261216975 20.261799988480163 44-45 20.021638890826598 28.929235396206593 30.916436521316555 20.13268919165025 46-47 20.472837622688303 28.69439068623515 30.145874042155125 20.686897648921423 48-49 20.16580502518209 28.790709487732 30.32115798997398 20.72232749711193 50-51 19.985411578646502 29.2950576104531 29.87946059358863 20.840070217311766 52-53 19.978688225642625 29.44789067509212 30.00158948756466 20.571831611700595 54-55 20.308803139110765 28.966780381868652 29.96088518541321 20.763531293607375 56-57 20.447499000386735 29.156143805722003 29.40871587854067 20.987641315350594 58-59 20.330243132381696 29.016666202344442 29.61702312956767 21.036067535706184 60-61 20.370653054936554 28.85928557146931 29.804847235612886 20.96521413798125 62-63 20.266301104855327 28.661253936004677 29.98189748023131 21.09054747890868 64-65 20.232595811580584 28.71788821486776 29.618289303476647 21.431226670075006 66-67 20.19905763996116 28.905845460070022 29.38259686485505 21.512500035113774 68-69 20.401852724073706 28.36600992002367 29.80199748862531 21.430139867277312 70-71 20.376393393799095 28.254159430778632 29.79866104950249 21.57078612591979 72-73 20.377930212923772 28.042361861307118 29.782122007742256 21.79758591802685 74-75 20.180578461699834 28.32941392425098 29.597110969381784 21.892896644667402 76-77 19.92806835051906 28.25815324082236 29.930533655391585 21.883244753266997 78-79 19.828999568355698 27.8877097477643 29.816228140399563 22.46706254348044 80-81 19.967167971895446 28.07683808796156 29.524808710014494 22.4311852301285 82-83 20.0106507241322 28.065734864852843 29.60540822704067 22.318206183974286 84-85 20.020776973607674 27.583796675918304 29.709340064236333 22.68608628623769 86-87 19.694005158594717 27.943262012881974 29.57174487195514 22.790987956568173 88-89 20.23428102717996 27.422405268665457 29.56883009111189 22.774483613042694 90-91 20.119405724946773 27.352167044385183 29.71248826629659 22.815938964371455 92-93 20.06598879707649 27.32964991098162 29.7578475252328 22.84651376670909 94-95 19.999419241013573 27.270147067846235 29.686057615974992 23.0443760751652 96-97 20.326174183270776 26.884197299647184 29.425738892522364 23.36388962455968 98-99 20.212146270700252 26.59950215442765 29.306139439839445 23.882212135032653 100-101 20.465109197361038 26.28019221827526 29.83192027368734 23.42277831067636 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 235.0 1 154.0 2 87.5 3 104.0 4 98.0 5 120.0 6 210.0 7 396.5 8 674.5 9 868.0 10 922.0 11 967.5 12 1099.5 13 1255.0 14 1426.0 15 1678.5 16 1991.5 17 2276.5 18 2640.0 19 3182.5 20 3791.5 21 4541.5 22 5439.0 23 6429.0 24 7866.5 25 10179.5 26 13799.5 27 18204.5 28 21712.5 29 26126.0 30 33094.0 31 40829.5 32 49747.0 33 59647.5 34 70977.0 35 82093.5 36 90790.5 37 96611.0 38 95738.5 39 92366.5 40 90816.5 41 89881.0 42 88033.5 43 87638.5 44 87872.5 45 85439.5 46 81047.0 47 76383.5 48 74192.0 49 71418.5 50 66496.0 51 60492.0 52 54035.5 53 47598.0 54 41490.5 55 35877.0 56 30443.5 57 25221.0 58 20550.0 59 16654.0 60 13318.0 61 10392.5 62 7845.0 63 5557.0 64 3800.5 65 2723.5 66 2063.5 67 1623.5 68 1272.0 69 1053.0 70 915.0 71 728.0 72 580.5 73 549.0 74 456.5 75 333.5 76 254.5 77 195.0 78 156.5 79 135.0 80 117.0 81 88.0 82 54.0 83 32.5 84 18.0 85 8.5 86 6.0 87 5.0 88 1.5 89 2.0 90 3.0 91 2.5 92 2.0 93 1.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007630625303996154 2 0.10631380408205685 3 0.11445937955994231 4 0.11661280755984307 5 0.15808970338401848 6 0.09484445929997673 7 0.02541981312926326 8 0.02701147730310295 9 0.021815161912038084 10-11 0.051401390084587585 12-13 0.05362503856274598 14-15 0.026660374911814784 16-17 0.03056931486815637 18-19 0.03599969852008001 20-21 0.05823618330166389 22-23 0.03056931486815637 24-25 0.0468604658239273 26-27 0.05641045086696543 28-29 0.052735579171482624 30-31 0.06720099769255508 32-33 0.048709605084711644 34-35 0.061957868649318466 36-37 0.053203715693200176 38-39 0.031131078694217437 40-41 0.03574222343313536 42-43 0.03192691078113728 44-45 0.007490184347480887 46-47 0.013084415782005674 48-49 0.00873074613003241 50-51 0.00882437343437592 52-53 0.010322410303872098 54-55 0.011118242390791943 56-57 0.01418453660804193 58-59 0.01870205404261634 60-61 0.024343099129312883 62-63 0.011165056042963698 64-65 0.008988221216977064 66-67 0.010064935216927442 68-69 0.01156297208642362 70-71 0.03045228073772698 72-73 0.026964663650931198 74-75 0.028228632259568592 76-77 0.022962096390246097 78-79 0.005196315391064865 80-81 0.004915433478034332 82-83 0.0058048928692976875 84-85 0.005172908564978988 86-87 0.01292056799940453 88-89 0.03686575108525749 90-91 0.033729236389749874 92-93 0.02879039608562966 94-95 0.0464391429543815 96-97 0.04164074360677656 98-99 0.02467079469451517 100-101 0.07380172264877262 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2136129.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.75608240743051 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65146742559688 43.504370018576516 2 13.777380190947927 15.639002509518827 3 4.634205905685734 7.8905811682830205 4 1.9503264063746633 4.427715449663528 5 0.9690071869160766 2.7498525877000626 6 0.5366342692309847 1.8274355284275008 7 0.3403836016889485 1.3523187823317175 8 0.20352580385466362 0.9241061836491062 9 0.14095164794108325 0.719987701140653 >10 0.6379470558016814 6.865183813990053 >50 0.08467557659574461 3.38316392120602 >100 0.06836561532542001 7.632995283827484 >500 0.004219274452381732 1.5868844772521955 >1k 7.445778445379417E-4 0.6921137766214077 >5k 1.6546174323065372E-4 0.8042887978119466 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9694 0.45381154415299824 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7435 0.34805950389700246 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4889 0.22887194546771286 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 4.6813652171755545E-5 0.0 10-11 0.0 0.0 0.0 9.362730434351109E-5 0.0 12-13 0.0 0.0 0.0 1.1703413042938885E-4 0.0 14-15 0.0 0.0 0.0 1.4044095651526663E-4 0.0 16-17 0.0 0.0 0.0 1.638477826011444E-4 0.0 18-19 0.0 0.0 0.0 2.340682608587777E-4 0.0 20-21 0.0 0.0 0.0 4.4472969563167763E-4 0.0 22-23 0.0 0.0 0.0 0.0011001208260362552 0.0 24-25 0.0 0.0 0.0 0.0023172757825018994 0.0 26-27 0.0 0.0 0.0 0.0037450921737404433 0.0 28-29 0.0 0.0 0.0 0.01029900347778622 0.0 30-31 0.0 0.0 0.0 0.03363560908540636 0.0 32-33 0.0 0.0 0.0 0.08304741895269432 0.0 34-35 0.0 0.0 0.0 0.16307535734031042 0.0 36-37 0.0 0.0 0.0 0.2857973465085676 0.0 38-39 0.0 0.0 0.0 0.4600143530657559 0.0 40-41 0.0 0.0 0.0 0.646332688709343 0.0 42-43 0.0 0.0 0.0 0.8364195233527563 0.0 44-45 0.0 0.0 0.0 1.0723369234723183 0.0 46-47 0.0 0.0 0.0 1.3492396760682523 0.0 48-49 0.0 0.0 0.0 1.6286235522292896 0.0 50-51 0.0 0.0 0.0 1.9221685581722827 0.0 52-53 0.0 0.0 0.0 2.211476928593732 0.0 54-55 0.0 0.0 0.0 2.541255701317664 0.0 56-57 0.0 0.0 0.0 2.928100316038966 0.0 58-59 0.0 0.0 0.0 3.3352386489767234 0.0 60-61 0.0 0.0 0.0 3.7570062482181554 0.0 62-63 0.0 0.0 0.0 4.177486472024864 0.0 64-65 0.0 0.0 0.0 4.627248635265005 0.0 66-67 0.0 0.0 0.0 5.1293718684592555 0.0 68-69 0.0 0.0 0.0 5.669975923738688 0.0 70-71 0.0 0.0 0.0 6.259640686494121 0.0 72-73 0.0 0.0 0.0 6.844694304510636 0.0 74-75 0.0 0.0 0.0 7.440327807917967 0.0 76-77 0.0 0.0 0.0 8.090826911670597 0.0 78-79 0.0 0.0 0.0 8.797502398029334 0.0 80-81 0.0 0.0 0.0 9.52875505177824 0.0 82-83 0.0 0.0 0.0 10.260265180614091 0.0 84-85 0.0 0.0 0.0 10.99804833884096 0.0 86-87 0.0 4.6813652171755545E-5 0.0 11.77534221950079 0.0 88-89 0.0 9.362730434351109E-5 0.0 12.594370471071738 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5180 0.0 47.655453 1 GTATCAA 8860 0.0 38.81321 1 TCAACGC 11035 0.0 30.7752 4 CAACGCA 11210 0.0 30.252337 5 TATCAAC 11400 0.0 29.915022 2 AACGCAG 11550 0.0 29.338396 6 ATCAACG 11735 0.0 28.939442 3 GTACATG 9110 0.0 26.470648 1 ACGCAGA 13025 0.0 25.907757 7 CGCAGAG 12975 0.0 25.85986 8 TACATGG 9485 0.0 24.972788 2 ACATGGG 9480 0.0 24.23337 3 GCAGAGT 13585 0.0 24.03174 9 GAGTACT 8030 0.0 23.019005 12-13 TGTAGGA 2280 0.0 22.112917 2 GTCGGGA 345 0.0 22.058496 2 AGTACTT 8535 0.0 21.378645 12-13 GTGTAGC 1460 0.0 21.175589 1 GTACTTT 8825 0.0 20.754696 14-15 AGAGTAC 12830 0.0 20.64959 10-11 >>END_MODULE