FastQCFastQC Report
Thu 26 May 2016
SRR938527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136129
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93610.4382225979798036No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73720.3451102438101819No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51730.24216702268449142No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52300.045.9052121
GTATCAA87650.036.6662941
TAAATCG600.003948434731.6720225
TCAACGC111600.028.3515684
CAACGCA113850.027.749535
TATCAAC115750.027.5185832
AACGCAG116900.027.1334676
CTCCGAC3200.026.72514794-95
ATCAACG118900.026.5709363
ACGCAGA131950.024.0386687
CGCAGAG131650.024.021318
GTACATG87700.023.7978211
TACATGG90050.022.8600122
CGTTAAC1050.0021857322.6387792
GCAGAGT138800.022.4418019
GAGTACT80450.022.01537712-13
ACATGGG91400.021.8828033
AGTACTT84500.021.80310612-13
GTGTAGC14500.021.3116781
AGAGTAC130850.020.3760210-11
Produced by FastQC (version 0.11.5)