##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938527_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2136129 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.807697475199298 31.0 31.0 34.0 30.0 34.0 2 32.03722200297828 31.0 31.0 34.0 30.0 34.0 3 32.09404066889219 33.0 31.0 34.0 30.0 34.0 4 35.770609359266224 37.0 35.0 37.0 35.0 37.0 5 35.67131151723515 37.0 35.0 37.0 33.0 37.0 6 35.668993773316124 37.0 35.0 37.0 33.0 37.0 7 35.614264400698644 37.0 35.0 37.0 33.0 37.0 8 35.59618262754731 37.0 35.0 37.0 33.0 37.0 9 37.17262346983726 39.0 37.0 39.0 33.0 39.0 10-11 37.084317941472634 39.0 37.0 39.0 33.0 39.0 12-13 36.99963438537654 39.0 37.0 39.0 33.0 39.0 14-15 38.04039807520988 40.0 37.0 41.0 33.0 41.0 16-17 37.96717894846238 40.0 37.0 41.0 33.0 41.0 18-19 38.22917248911466 40.0 37.5 41.0 33.0 41.0 20-21 38.231092316990214 40.0 38.0 41.0 33.0 41.0 22-23 38.11960396586535 40.0 37.0 41.0 33.0 41.0 24-25 38.02729704058135 40.0 37.0 41.0 33.0 41.0 26-27 37.862464298738516 40.0 37.0 41.0 32.5 41.0 28-29 37.75254069393749 40.0 37.0 41.0 32.5 41.0 30-31 37.51496468612149 39.0 36.5 41.0 32.0 41.0 32-33 37.323578538562046 39.0 36.0 41.0 31.5 41.0 34-35 37.21279309442454 39.0 36.0 41.0 31.0 41.0 36-37 37.1725305447377 39.0 36.0 41.0 31.0 41.0 38-39 37.02575921210751 39.0 36.0 41.0 31.0 41.0 40-41 36.87290116842195 39.0 35.5 41.0 31.0 41.0 42-43 36.67517996338236 39.0 35.0 41.0 30.0 41.0 44-45 36.345242258309305 39.0 35.0 41.0 30.0 41.0 46-47 35.93348903554046 39.0 35.0 40.0 29.5 41.0 48-49 35.584725220246526 39.0 35.0 40.0 27.5 41.0 50-51 35.25648123310905 38.5 35.0 40.0 26.5 41.0 52-53 34.85662733851748 38.0 34.0 40.0 26.0 41.0 54-55 34.5845435832761 38.0 34.0 40.0 24.0 41.0 56-57 34.31291204791471 38.0 33.0 40.0 24.0 41.0 58-59 33.90948720793547 38.0 33.0 40.0 23.0 41.0 60-61 33.59425413914609 37.5 33.0 40.0 22.0 41.0 62-63 33.16633850296494 37.0 32.0 39.5 20.5 41.0 64-65 32.71529247531399 36.0 31.0 39.0 19.0 40.0 66-67 32.27150981986575 36.0 31.0 39.0 18.0 40.0 68-69 31.82225862763906 35.5 31.0 38.5 14.5 40.0 70-71 31.326768420821026 35.0 30.0 38.0 12.0 40.0 72-73 30.868975843687345 34.5 29.5 37.5 8.0 39.5 74-75 30.29599195554201 34.0 29.0 37.0 7.5 39.0 76-77 28.968643981707096 32.5 27.5 35.5 7.0 38.0 78-79 29.685267135084068 34.0 29.0 36.0 4.0 38.0 80-81 29.315680607304145 34.0 29.0 35.5 2.0 37.5 82-83 28.890200685445492 34.0 28.5 35.0 2.0 37.0 84-85 28.358364827217834 33.0 27.5 35.0 2.0 36.5 86-87 27.786726363435918 33.0 26.0 35.0 2.0 36.0 88-89 27.103652447956094 32.0 26.0 35.0 2.0 35.5 90-91 26.546213033014393 32.0 25.0 34.0 2.0 35.0 92-93 25.806452232051527 32.0 23.5 34.0 2.0 35.0 94-95 25.070137149956768 31.0 20.0 34.0 2.0 35.0 96-97 24.086140631019944 31.0 12.0 34.0 2.0 35.0 98-99 22.826975805300147 31.0 2.0 34.0 2.0 35.0 100-101 18.871003811099424 24.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.34349113673188114 1101 2 0.2921138852166152 1101 3 0.3065589823168082 1101 4 0.1720335911133617 1101 5 0.10598299070125705 1101 6 0.149464255327608 1101 7 0.17754892167656777 1101 8 0.25398476988077334 1101 9 0.2911323041738072 1101 10-11 0.3135994712461141 1101 12-13 0.3279215388565362 1101 14-15 0.34675860553395665 1101 16-17 0.5442505630166252 1101 18-19 0.40603255727816645 1101 20-21 0.40617366260546106 1101 22-23 0.36690492659132445 1101 24-25 0.5932608486506084 1101 26-27 0.4331105600509062 1101 28-29 0.4687553808529472 1101 30-31 0.49015392900722077 1101 32-33 0.46994710911839377 1101 34-35 0.5220397089537627 1101 36-37 0.55472253835012 1101 38-39 0.5193524340467448 1101 40-41 0.5103636200558412 1101 42-43 0.663886551083614 1101 44-45 0.7007116051612741 1101 46-47 0.6376141281083605 1101 48-49 0.5582366480869041 1101 50-51 0.578751825393752 1101 52-53 0.6655894333163559 1101 54-55 0.7454518319428658 1101 56-57 0.6825305416912002 1101 58-59 0.9224474048925586 1101 60-61 0.812251245223095 1101 62-63 0.895763291731619 1101 64-65 0.7642292940810478 1101 66-67 0.48942050465530684 1101 68-69 0.7434172290146854 1101 70-71 0.8463344026303901 1101 72-73 0.6947686679392469 1101 74-75 0.7002277418924976 1101 76-77 0.7808979289158913 1101 78-79 0.8945599929153083 1101 80-81 0.9351138072530567 1101 82-83 0.8757996850903425 1101 84-85 0.8807606680209794 1101 86-87 0.9817959414592252 1101 88-89 0.9923279342839102 1101 90-91 0.9416357368598973 1101 92-93 1.31214936155477 1101 94-95 1.012580539897563 1101 96-97 0.8807089871167797 1101 98-99 1.232889571973498 1101 100-101 1.399614924657815 1102 1 0.31214127547799464 1102 2 0.3213031486496618 1102 3 0.338237198640563 1102 4 0.19913844368622335 1102 5 0.16301644214226485 1102 6 0.2233580957727952 1102 7 0.26523421158405114 1102 8 0.28417036560914966 1102 9 0.3170728912211658 1102 10-11 0.31836491198232153 1102 12-13 0.3256997768560126 1102 14-15 0.5054402170062176 1102 16-17 0.6403215156111628 1102 18-19 0.5054517121823494 1102 20-21 0.4704427291971598 1102 22-23 0.4444530585085076 1102 24-25 0.5116474448962052 1102 26-27 0.5478526832849226 1102 28-29 0.4828502368381251 1102 30-31 0.49081385831430424 1102 32-33 0.4957645630145535 1102 34-35 0.5408766667118456 1102 36-37 0.6131879827347149 1102 38-39 0.7652597992870369 1102 40-41 0.5866954432420002 1102 42-43 0.5029051128025586 1102 44-45 0.7323581663394023 1102 46-47 0.6888326902565325 1102 48-49 0.6207239055494753 1102 50-51 0.6031839642729295 1102 52-53 0.4646624204246592 1102 54-55 0.4255556608205069 1102 56-57 0.5084650659733754 1102 58-59 0.6049929722064249 1102 60-61 0.5586224388259708 1102 62-63 0.6359418804057313 1102 64-65 0.6102138656849903 1102 66-67 0.47969164439077616 1102 68-69 0.6551135907117462 1102 70-71 0.627537255241883 1102 72-73 0.6268893051626918 1102 74-75 0.613223037615942 1102 76-77 0.560219714738249 1102 78-79 0.5805768956900152 1102 80-81 0.5485409006239301 1102 82-83 0.7497333359051126 1102 84-85 0.8556305063543981 1102 86-87 0.7314447839539504 1102 88-89 0.8595968356250161 1102 90-91 0.8342797248502585 1102 92-93 1.103344777889685 1102 94-95 1.1700179431884088 1102 96-97 1.2064109265578047 1102 98-99 1.403434351664412 1102 100-101 1.6969570727867271 1103 1 0.23741683101108535 1103 2 0.2026886838161559 1103 3 0.2319915259110843 1103 4 0.2279804281914366 1103 5 0.17389488437541445 1103 6 0.11707022425925118 1103 7 0.22788373931143013 1103 8 0.18522988358279946 1103 9 0.3402400236748093 1103 10-11 0.34183122623439033 1103 12-13 0.35346455527449194 1103 14-15 0.5494966319794585 1103 16-17 0.4782489994012664 1103 18-19 0.5295292770850892 1103 20-21 0.5976298643798259 1103 22-23 0.3881293053941377 1103 24-25 0.6342768587121341 1103 26-27 0.5356548341594731 1103 28-29 0.48455979296756624 1103 30-31 0.32686829727262534 1103 32-33 0.3464418914019589 1103 34-35 0.36985914537239495 1103 36-37 0.3333536950855702 1103 38-39 0.4697025479187289 1103 40-41 0.41427095885050136 1103 42-43 0.5808381809826599 1103 44-45 0.5797438632257936 1103 46-47 0.5417679062789276 1103 48-49 0.5382563632369752 1103 50-51 0.7095414511458245 1103 52-53 0.6133289893855647 1103 54-55 0.5330839744358116 1103 56-57 0.6851648936406178 1103 58-59 0.7669152657987794 1103 60-61 0.7886678467391235 1103 62-63 0.8430048703032895 1103 64-65 0.664027043834615 1103 66-67 0.6974017412711007 1103 68-69 0.8749036833469184 1103 70-71 0.8451616911174185 1103 72-73 0.6688993317959344 1103 74-75 0.8134419911871724 1103 76-77 0.8535108661333339 1103 78-79 1.1162755508880444 1103 80-81 0.9529135285216235 1103 82-83 1.1004742151559483 1103 84-85 1.0982799582755796 1103 86-87 1.1727524661541509 1103 88-89 1.2463168530099011 1103 90-91 1.3274119359149275 1103 92-93 1.4408298061654001 1103 94-95 1.2697782692561397 1103 96-97 1.463169301879347 1103 98-99 1.604219324854288 1103 100-101 1.7257646918150975 1104 1 0.25427172325026604 1104 2 0.2587573823832301 1104 3 0.2508819664945321 1104 4 0.18555794485100563 1104 5 0.15819670509204542 1104 6 0.12678761230527869 1104 7 0.2440108682984814 1104 8 0.2129717239031237 1104 9 0.2976264995068334 1104 10-11 0.3005075250384195 1104 12-13 0.3398455806141669 1104 14-15 0.4702465683388297 1104 16-17 0.38580224935547136 1104 18-19 0.3438701862300704 1104 20-21 0.3739254566420769 1104 22-23 0.2734336741148269 1104 24-25 0.3193476009164513 1104 26-27 0.19717592834997788 1104 28-29 0.31313110258455623 1104 30-31 0.32843123963444754 1104 32-33 0.1834021059005977 1104 34-35 0.3772955820684203 1104 36-37 0.3576970071101968 1104 38-39 0.3223599900652374 1104 40-41 0.44002566528283893 1104 42-43 0.4782006042273039 1104 44-45 0.40729026140436275 1104 46-47 0.39188162672172666 1104 48-49 0.5709570572171643 1104 50-51 0.7641154871985378 1104 52-53 0.572714256640495 1104 54-55 0.4937383665905273 1104 56-57 0.3902316389758127 1104 58-59 0.610734869014351 1104 60-61 0.7332042693985201 1104 62-63 0.5874279293832032 1104 64-65 0.462416162834252 1104 66-67 0.4241643486572144 1104 68-69 0.5312485941191945 1104 70-71 0.6539703862110287 1104 72-73 0.6014754804794684 1104 74-75 0.783619756699423 1104 76-77 0.7551820570337959 1104 78-79 1.0563049088433587 1104 80-81 0.8644182069996731 1104 82-83 1.109255550728797 1104 84-85 1.2206175795198888 1104 86-87 1.172289138617863 1104 88-89 1.2462678437814176 1104 90-91 1.1529559740374502 1104 92-93 1.420110560051043 1104 94-95 1.5014015928907192 1104 96-97 1.3822602996453845 1104 98-99 1.684025500492897 1104 100-101 2.0606541474557964 1105 1 0.1347563957464324 1105 2 0.20992649091967763 1105 3 0.18207427715464775 1105 4 0.15396500730782492 1105 5 0.02136448677921976 1105 6 0.13489904281021836 1105 7 0.21078464670578967 1105 8 0.24109764686603796 1105 9 0.22442983046614984 1105 10-11 0.22915789049847746 1105 12-13 0.2731323796169036 1105 14-15 0.32552953047662214 1105 16-17 0.2871892325738443 1105 18-19 0.17987015252668215 1105 20-21 0.22226884629850474 1105 22-23 0.2210761219388715 1105 24-25 0.24466931308417372 1105 26-27 0.1728950744479647 1105 28-29 0.19041550509881944 1105 30-31 0.21826882476876364 1105 32-33 0.28161312105559233 1105 34-35 0.319672983767596 1105 36-37 0.2588411928761829 1105 38-39 0.15873645871266007 1105 40-41 0.20424095722072622 1105 42-43 0.19526019625663338 1105 44-45 0.23568634365127394 1105 46-47 0.18288379704269886 1105 48-49 0.31512836623025464 1105 50-51 0.1585107907197454 1105 52-53 0.4167538120040817 1105 54-55 0.21788276610696755 1105 56-57 0.16959882233911827 1105 58-59 0.17410234329112484 1105 60-61 0.2544307535228896 1105 62-63 0.1831559802717706 1105 64-65 0.24043410190738967 1105 66-67 0.19666845736661287 1105 68-69 0.40081159418216217 1105 70-71 0.4035173860327532 1105 72-73 0.23974541448290765 1105 74-75 0.5730265592853634 1105 76-77 0.3132068786940785 1105 78-79 0.54782030198011 1105 80-81 0.4892688699364989 1105 82-83 0.7148943940639043 1105 84-85 0.6111387669790354 1105 86-87 0.6324625957786374 1105 88-89 0.5999895850404897 1105 90-91 0.8299850290209818 1105 92-93 0.9019417716968086 1105 94-95 0.8954798184604194 1105 96-97 1.0669783840865676 1105 98-99 1.269799742765283 1105 100-101 1.522380843113293 1106 1 -0.09448109138804384 1106 2 0.03281961204522332 1106 3 0.10103858686736089 1106 4 0.026058130671785307 1106 5 -0.04232109466498457 1106 6 0.00768586059307097 1106 7 0.033878795007019846 1106 8 0.12150886151356133 1106 9 -0.2668455040159543 1106 10-11 0.012893651252888105 1106 12-13 -0.021753071482486064 1106 14-15 0.048708222112558985 1106 16-17 0.10487792369752924 1106 18-19 0.043098171152038844 1106 20-21 0.20906885094753136 1106 22-23 0.15883378217195343 1106 24-25 0.23368578224630454 1106 26-27 0.1898242960939882 1106 28-29 0.24667362743613097 1106 30-31 0.027855195212346473 1106 32-33 0.2444551631989853 1106 34-35 0.21018882724879262 1106 36-37 0.30441484996514845 1106 38-39 0.12765494542141198 1106 40-41 0.25461634426260815 1106 42-43 0.3000382936786181 1106 44-45 0.30604682368867486 1106 46-47 -0.3720034017447702 1106 48-49 -0.02584073541932952 1106 50-51 0.41205459419695245 1106 52-53 0.14736189983960912 1106 54-55 0.29606877780098273 1106 56-57 0.19207329468582657 1106 58-59 0.22742641588647672 1106 60-61 0.44013757066866077 1106 62-63 0.2407503737228467 1106 64-65 0.315901942079158 1106 66-67 0.09166694858663504 1106 68-69 -0.15614151396916043 1106 70-71 -0.1449520094092236 1106 72-73 0.22686546989236334 1106 74-75 0.2722401857424295 1106 76-77 0.24640397701530858 1106 78-79 0.5714578908765233 1106 80-81 -0.1299095746125012 1106 82-83 0.30408083234877736 1106 84-85 0.4500898485009337 1106 86-87 0.7450395360244926 1106 88-89 0.656621028187466 1106 90-91 0.7704274997353657 1106 92-93 0.8683196537140851 1106 94-95 0.938525461960463 1106 96-97 0.9602691128595815 1106 98-99 0.8973313614989529 1106 100-101 0.7111338461450565 1107 1 0.11573694460996364 1107 2 0.20159572493837885 1107 3 0.17238367152418732 1107 4 0.10987202158778331 1107 5 0.04263367867825707 1107 6 0.06194895172234993 1107 7 0.05050311136209018 1107 8 0.23236481093402261 1107 9 0.34976110114293135 1107 10-11 0.18628108925594944 1107 12-13 0.2420645065072975 1107 14-15 0.18270852543748362 1107 16-17 0.24990665369923448 1107 18-19 0.27977096156134706 1107 20-21 0.245592399700179 1107 22-23 0.30812385494304095 1107 24-25 0.296436384809752 1107 26-27 0.3243422794756725 1107 28-29 0.26312517686577763 1107 30-31 0.20666720431374586 1107 32-33 0.35896932294858885 1107 34-35 0.450705112755756 1107 36-37 0.4492647092997686 1107 38-39 0.40902181526335823 1107 40-41 0.43705097577558405 1107 42-43 0.4673425772017694 1107 44-45 0.498732947980308 1107 46-47 0.465141735507693 1107 48-49 0.47407443291668727 1107 50-51 0.5487933050163605 1107 52-53 0.5618035802431507 1107 54-55 0.3284826462469397 1107 56-57 0.38636542721109635 1107 58-59 0.314314109729402 1107 60-61 0.22151685490045736 1107 62-63 0.2839693132815242 1107 64-65 0.3354953666734346 1107 66-67 0.4179427702588896 1107 68-69 0.5561498285870989 1107 70-71 0.7638137085014876 1107 72-73 0.7557379267270186 1107 74-75 0.4371686654079703 1107 76-77 0.728628122548372 1107 78-79 1.0122881156121508 1107 80-81 1.051654187452474 1107 82-83 1.1197559030745907 1107 84-85 1.1719585824669743 1107 86-87 1.1893691703524354 1107 88-89 1.151226561984501 1107 90-91 1.2924659384106612 1107 92-93 1.5739871951124549 1107 94-95 1.506471279769773 1107 96-97 1.7326309981171057 1107 98-99 1.8261258352746914 1107 100-101 2.1270828403829185 1108 1 0.13524997584884346 1108 2 0.10460346130697218 1108 3 0.12187071993774623 1108 4 0.08407108612541236 1108 5 0.0017584698426844625 1108 6 0.08540971886602478 1108 7 0.17186397822254662 1108 8 0.09307186239252019 1108 9 0.1707504246921232 1108 10-11 0.17357894600053925 1108 12-13 0.2050870239476552 1108 14-15 0.32194420488785624 1108 16-17 0.3337736079212661 1108 18-19 0.3084655993393852 1108 20-21 0.15910024244006138 1108 22-23 0.2153244348934109 1108 24-25 0.20594456632453984 1108 26-27 0.1919818149104202 1108 28-29 0.2981202212745373 1108 30-31 0.313997143848475 1108 32-33 0.47437974975456143 1108 34-35 0.43900326290361846 1108 36-37 0.4295258281240564 1108 38-39 0.5025232858085928 1108 40-41 0.4365961503001117 1108 42-43 0.35938963464931817 1108 44-45 0.36691603565142117 1108 46-47 0.3872954969678162 1108 48-49 0.49674119637618475 1108 50-51 0.4417753207068813 1108 52-53 0.48705551887245946 1108 54-55 0.3337755000121305 1108 56-57 0.36779179055322686 1108 58-59 0.5850907411171065 1108 60-61 0.40689508800783614 1108 62-63 0.570714845939527 1108 64-65 0.3909571541748562 1108 66-67 0.41564192402741185 1108 68-69 0.5600254739986674 1108 70-71 0.5671459025025527 1108 72-73 0.6085993143530608 1108 74-75 0.5430689950077188 1108 76-77 0.38024179009928005 1108 78-79 0.5661604841831185 1108 80-81 0.6667474160882314 1108 82-83 0.6599510414530485 1108 84-85 0.9116637071431981 1108 86-87 1.226448386209519 1108 88-89 1.1384287349835347 1108 90-91 1.114688765588074 1108 92-93 1.226934587201054 1108 94-95 1.4711393761472031 1108 96-97 1.4736130823692086 1108 98-99 1.5419848363525155 1108 100-101 1.6145455474162311 1109 1 0.17115025356292435 1109 2 0.23230225618072353 1109 3 0.23296781795298216 1109 4 0.14144452239130345 1109 5 0.19284359303053833 1109 6 0.24362457784332037 1109 7 0.1521042391209093 1109 8 0.3000898755903094 1109 9 0.32157337559532806 1109 10-11 0.25326664505019636 1109 12-13 0.2607645571332071 1109 14-15 0.41903234726091654 1109 16-17 0.36533810637983066 1109 18-19 0.4187063061089873 1109 20-21 0.48269320022242823 1109 22-23 0.47270647010449807 1109 24-25 0.4182614406398102 1109 26-27 0.3841599595765217 1109 28-29 0.3827363898393443 1109 30-31 0.49149083795533244 1109 32-33 0.5270696272036659 1109 34-35 0.5551304253790903 1109 36-37 0.6159926807827247 1109 38-39 0.5627213163316611 1109 40-41 0.505745364292828 1109 42-43 0.5779082593276499 1109 44-45 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-1.1616707115691973 2316 70-71 -1.2623014878574566 2316 72-73 -1.1584930164314358 2316 74-75 -0.9772860406695685 2316 76-77 -1.1822055533834437 2316 78-79 -1.4509991752488212 2316 80-81 -1.4297104076844356 2316 82-83 -1.3747755582802093 2316 84-85 -1.4460247648136182 2316 86-87 -1.5885779891561924 2316 88-89 -1.8296133663320617 2316 90-91 -1.5959547675987444 2316 92-93 -1.774137891513444 2316 94-95 -1.9982575705790673 2316 96-97 -2.1569448400295848 2316 98-99 -2.173231713718394 2316 100-101 -2.299801566919303 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 13.0 4 21.0 5 64.0 6 134.0 7 353.0 8 797.0 9 1558.0 10 2682.0 11 4272.0 12 6135.0 13 8534.0 14 11292.0 15 15291.0 16 20021.0 17 25565.0 18 23295.0 19 16987.0 20 15664.0 21 16424.0 22 18196.0 23 20861.0 24 23934.0 25 27962.0 26 33560.0 27 40392.0 28 48878.0 29 58753.0 30 70485.0 31 84532.0 32 102632.0 33 126840.0 34 160449.0 35 210267.0 36 295019.0 37 395583.0 38 238047.0 39 10633.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 39.70220746232184 22.061562142166302 15.538373816427745 22.697856579084114 2 14.858781645384344 22.185527396140657 41.228050043395235 21.727640915079764 3 17.56504405866874 26.56473462042789 31.97798447565667 23.892236845246707 4 11.831225390625184 18.96376011351385 39.590670404896336 29.61434409096463 5 11.903656823683857 39.211599698195776 36.03937692696422 12.845366551156143 6 28.82611490223671 39.395045898445275 18.59943851705585 13.179400682262166 7 26.06055158653808 34.45077521067314 22.36625222540399 17.12242097738479 8 24.11689556201896 36.9630298544704 21.20260527337066 17.717469310139975 9 25.499724033520447 16.839057940789157 22.43207222035748 35.22914580533292 10-11 23.07891985924071 27.800591630936143 29.721847322891083 19.39864118693206 12-13 24.339049748400026 25.948456296412807 29.14074945848308 20.571744496704085 14-15 21.1548085345033 28.05464932127226 27.68229353189812 23.108248612326314 16-17 19.803930380609035 30.569923445634608 29.1075585790933 20.518587594663057 18-19 19.57143037709801 28.879459058886425 31.86677864492266 19.682331919092903 20-21 20.86397872038627 28.254894718436947 31.454678064854697 19.426448496322084 22-23 20.245523561545205 28.845448940583644 30.96613079079026 19.94289670708089 24-25 19.887386559655653 28.883399864090453 31.599928744634486 19.629284831619405 26-27 19.910603038588487 29.083482183924325 31.360141342326088 19.645773435161104 28-29 19.584752959329176 29.020245060450556 31.388316981301667 20.006684998918605 30-31 20.294112139469778 28.574474858269078 31.524865525328988 19.60654747693215 32-33 19.647796003969813 28.894490946201522 31.534183472838606 19.923529576990056 34-35 20.027000751217365 29.06520608848914 31.426472581502633 19.481320578790857 36-37 19.906348955498192 28.679391502338348 31.058502636862222 20.355756905301234 38-39 19.648260167534396 29.082591656800272 31.216798944214037 20.0523492314513 40-41 20.396108620077843 28.462796321905376 31.441955595836536 19.69913946218025 42-43 19.787996618132688 28.810522738188975 31.1542588401843 20.247221803494035 44-45 19.99569741849792 28.991767977089104 30.895133588367017 20.11740101604596 46-47 20.452556938274796 28.72605072071958 30.122244489916106 20.699147851089517 48-49 20.18864497415652 28.802193125976945 30.292833438429984 20.71632846143655 50-51 20.00229856323413 29.284936171567384 29.884856494283785 20.827908770914703 52-53 20.07219601437928 29.45252838194697 29.84382029362459 20.63145531004916 54-55 20.323829457839743 29.05340988116859 29.815899912620864 20.806860748370802 56-57 20.50330293722898 29.219302766827283 29.214808656218793 21.062585639724944 58-59 20.26125014983967 29.205344031885883 29.452837026881234 21.08056879139321 60-61 20.336880403758386 29.084947585094344 29.573869368376158 21.004302642771105 62-63 20.163957835400755 28.956278690865506 29.735078409019085 21.144685064714654 64-65 20.176964798070223 29.00261355038318 29.289205128589302 21.53121652295729 66-67 20.112384011086245 29.289366005299676 29.014246388074795 21.584003595539283 68-69 20.290968954618275 28.789744917559812 29.38522367091254 21.53406245690938 70-71 20.356285079558724 28.7370153406177 29.25536011092976 21.651339468893813 72-73 20.437960646553016 28.610013070377804 29.16721282619768 21.7848134568715 74-75 20.209164955622096 28.933314915704226 28.90433056210448 21.953189566569193 76-77 19.883758835883476 28.773880069918935 29.470914885126486 21.871446209071102 78-79 19.780362065663326 28.55405533866331 29.23315674449032 22.43242585118304 80-81 19.81637998428041 28.814570849731325 29.04195941891357 22.3270897470747 82-83 19.88056767234809 28.83675123275957 29.034259699164284 22.24842139572806 84-85 19.912083682737133 28.390153297552512 29.0163879161768 22.681375103533554 86-87 19.501516458182927 28.854420950813193 28.91097795868529 22.733084632318583 88-89 19.933059423759104 28.39410555592258 28.81089697186078 22.861938048457535 90-91 19.753621736129517 28.375783979793763 29.08952517772706 22.781069106349662 92-93 19.667980584360915 28.53182106370206 28.97097206496728 22.82922628696974 94-95 19.574778239127593 28.489370977855057 28.913623420233282 23.02222736278407 96-97 19.817311972507582 28.245552865561628 28.678306516781525 23.258828645149265 98-99 19.653328237216733 28.056370169169526 28.548838642802977 23.741462950810764 100-101 19.4284146440245 28.382688471044993 29.04380556227962 23.14509132265089 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 252.0 1 157.5 2 81.5 3 106.5 4 107.0 5 113.5 6 178.0 7 380.0 8 684.5 9 865.5 10 908.5 11 927.5 12 992.0 13 1135.0 14 1356.5 15 1594.0 16 1870.0 17 2183.5 18 2602.0 19 3136.5 20 3734.5 21 4454.0 22 5301.5 23 6423.5 24 7837.0 25 10073.5 26 13868.0 27 18518.0 28 21930.5 29 26153.0 30 32902.5 31 40403.0 32 49880.5 33 60305.0 34 71399.5 35 82441.0 36 91583.5 37 97267.5 38 96111.0 39 92798.5 40 91326.5 41 90620.5 42 89146.5 43 89094.0 44 89587.5 45 87139.0 46 82234.5 47 77701.5 48 75878.0 49 72672.0 50 67519.0 51 60977.5 52 53440.0 53 46334.0 54 39930.0 55 34346.5 56 28892.0 57 23710.0 58 19343.5 59 15575.5 60 12210.5 61 9485.0 62 7089.5 63 4772.0 64 3128.0 65 2268.0 66 1703.0 67 1355.0 68 1091.5 69 912.5 70 773.5 71 611.0 72 507.0 73 454.0 74 359.5 75 258.0 76 191.5 77 158.0 78 120.0 79 89.5 80 82.5 81 63.0 82 39.0 83 26.5 84 13.0 85 3.0 86 4.0 87 2.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.14950173889311E-4 2 0.051588644693274614 3 0.0 4 9.362730434351109E-5 5 0.045409242606602875 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006998640999677454 26-27 0.0018023256086125883 28-29 1.4044095651526663E-4 30-31 0.0018257324346984663 32-33 4.2132286954579996E-4 34-35 0.01228858369508583 36-37 0.02534959265100563 38-39 0.004213228695458 40-41 0.0010767139999503777 42-43 0.0014512232173244219 44-45 0.009456357738694621 46-47 0.0 48-49 0.0 50-51 5.617638260610665E-4 52-53 0.0 54-55 0.0016618846520973219 56-57 0.0 58-59 0.024015403564110593 60-61 0.0 62-63 0.010018121564755686 64-65 0.01577620078188162 66-67 0.006975234173591576 68-69 0.01725083082529192 70-71 1.1703413042938887E-4 72-73 4.6813652171755545E-5 74-75 0.02312594417284724 76-77 0.050792812606354765 78-79 0.030897010433358657 80-81 0.02703488412918883 82-83 0.03639761456353994 84-85 0.01629115095577093 86-87 0.01081395365167553 88-89 0.08651162921340425 90-91 0.025794322346637307 92-93 0.024858049303202193 94-95 0.028462700520427372 96-97 0.03976819751990634 98-99 0.054771973040953985 100-101 0.01638477826011444 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2136129.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 55.08471933072756 #Duplication Level Percentage of deduplicated Percentage of total 1 75.35604605456075 41.50966646788859 2 14.435298883891729 15.903287749486813 3 4.847039999156813 8.00993513815089 4 2.1437059451400913 4.723417612626159 5 1.0619878936371068 2.9249652526815293 6 0.5836779432657974 1.9291041410599663 7 0.3527536102177281 1.3601933528221095 8 0.21937537969966525 0.9667384975062279 9 0.1572132929278531 0.7794045104391222 >10 0.6755008371019117 7.058881090732328 >50 0.08783241394277075 3.4256970738825596 >100 0.07370435459613646 7.997069333833514 >500 0.004758694844184239 1.7673271430187365 >1k 8.497669364614636E-4 0.6189108879381707 >5k 2.549300809384391E-4 1.0254017479332604 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9361 0.4382225979798036 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7372 0.3451102438101819 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5173 0.24216702268449142 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 4.6813652171755545E-5 0.0 0.0 9.362730434351109E-5 0.0 12-13 4.6813652171755545E-5 0.0 0.0 9.362730434351109E-5 0.0 14-15 4.6813652171755545E-5 0.0 0.0 9.362730434351109E-5 0.0 16-17 4.6813652171755545E-5 0.0 0.0 1.1703413042938885E-4 0.0 18-19 4.6813652171755545E-5 0.0 0.0 1.8725460868702218E-4 0.0 20-21 4.6813652171755545E-5 0.0 0.0 3.7450921737404436E-4 0.0 22-23 4.6813652171755545E-5 0.0 0.0 9.596798695209886E-4 0.0 24-25 4.6813652171755545E-5 0.0 0.0 0.0021768348259866327 0.0 26-27 4.6813652171755545E-5 0.0 0.0 0.003651464869396932 0.0 28-29 4.6813652171755545E-5 0.0 0.0 0.010228782999528587 0.0 30-31 4.6813652171755545E-5 0.0 0.0 0.03384627052017926 0.0 32-33 4.6813652171755545E-5 0.0 0.0 0.08379643738744241 0.0 34-35 4.6813652171755545E-5 0.0 0.0 0.16412866451417493 0.0 36-37 4.6813652171755545E-5 0.0 0.0 0.28715494242154854 0.0 38-39 4.6813652171755545E-5 0.0 0.0 0.4630104268047482 0.0 40-41 4.6813652171755545E-5 0.0 0.0 0.6515992245786655 0.0 42-43 4.6813652171755545E-5 0.0 0.0 0.8435351984828632 0.0 44-45 4.6813652171755545E-5 0.0 0.0 1.0812783310371237 0.0 46-47 4.6813652171755545E-5 0.0 0.0 1.360240884328615 0.0 48-49 4.6813652171755545E-5 0.0 0.0 1.640795101793946 0.0 50-51 4.6813652171755545E-5 0.0 0.0 1.9342698872586817 0.0 52-53 4.6813652171755545E-5 0.0 0.0 2.2245613443757373 0.0 54-55 4.6813652171755545E-5 0.0 0.0 2.5560488154039387 0.0 56-57 4.6813652171755545E-5 0.0 0.0 2.944368060168651 0.0 58-59 4.6813652171755545E-5 0.0 0.0 3.3517638681933537 0.0 60-61 4.6813652171755545E-5 0.0 0.0 3.7755444544781707 0.0 62-63 4.6813652171755545E-5 0.0 0.0 4.198107885806522 0.0 64-65 4.6813652171755545E-5 0.0 0.0 4.647589167133633 0.0 66-67 4.6813652171755545E-5 0.0 0.0 5.152568033110359 0.0 68-69 4.6813652171755545E-5 0.0 0.0 5.6969639942157055 0.0 70-71 4.6813652171755545E-5 0.0 0.0 6.2922698020578345 0.0 72-73 4.6813652171755545E-5 0.0 0.0 6.884719977117487 0.0 74-75 4.6813652171755545E-5 0.0 0.0 7.49119084100258 0.0 76-77 4.6813652171755545E-5 0.0 0.0 8.15451688545027 0.0 78-79 4.6813652171755545E-5 0.0 0.0 8.877132420373488 0.0 80-81 4.6813652171755545E-5 0.0 0.0 9.62692328038241 0.0 82-83 4.6813652171755545E-5 0.0 0.0 10.379101636652093 0.0 84-85 4.6813652171755545E-5 0.0 0.0 11.13830204074754 0.0 86-87 4.6813652171755545E-5 0.0 0.0 11.94216266901484 0.0 88-89 4.6813652171755545E-5 0.0 0.0 12.79002813032359 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5230 0.0 45.905212 1 GTATCAA 8765 0.0 36.666294 1 TAAATCG 60 0.0039484347 31.672022 5 TCAACGC 11160 0.0 28.351568 4 CAACGCA 11385 0.0 27.74953 5 TATCAAC 11575 0.0 27.518583 2 AACGCAG 11690 0.0 27.133467 6 CTCCGAC 320 0.0 26.725147 94-95 ATCAACG 11890 0.0 26.570936 3 ACGCAGA 13195 0.0 24.038668 7 CGCAGAG 13165 0.0 24.02131 8 GTACATG 8770 0.0 23.797821 1 TACATGG 9005 0.0 22.860012 2 CGTTAAC 105 0.00218573 22.638779 2 GCAGAGT 13880 0.0 22.441801 9 GAGTACT 8045 0.0 22.015377 12-13 ACATGGG 9140 0.0 21.882803 3 AGTACTT 8450 0.0 21.803106 12-13 GTGTAGC 1450 0.0 21.311678 1 AGAGTAC 13085 0.0 20.37602 10-11 >>END_MODULE